FastQCFastQC Report
Sat 31 Dec 2022
SRR5844264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23523
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC4191.7812353866428603No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT4031.7132168515920587No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA1170.49738553755898485No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT1000.42511584406750835No Hit
CAGTATGGGATTTAAGCACTTAGGCAGAGTAGACACAATCAAAGGTACAAAG950.40386005186413293No Hit
GGAACTGGGGATGGACCAGGAGCCAGTGGGCGGTGTGGAACGAGGAGAAGCC900.38260425966075756No Hit
AGGCACGGGATGGCCGCGGGCCGCCTCTTTCTAAGTCGGCTTCGAGCACCCT800.3400926752540067No Hit
AGAGCCGGGACCCCGTTTCTACCAAAAATACAAAAATTAGCCGGGCGTGGTG800.3400926752540067No Hit
TCTTCCATAGGGCTCCTGCTTCAGCCTGTCGAGTAGCTGGGATTATAGGCAT790.33584151681333163No Hit
ATACAGGGAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAA780.33159035837265655No Hit
TATTGGGGGAGCTAGGAATACCACTGATTTAGAAACAAAACTGTTTGTCTCT750.3188368830506313No Hit
AGCTAGGGAGACCTAGTTCTATTATCATTTGTTAGTTCACTGTGCTCAAGTC750.3188368830506313No Hit
CGTTACGGGGGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGG740.3145857246099562No Hit
ATGCGAGGGACAAGGAATATAGGCAGATGTACAGAACTGAAATTCGAATGGC730.3103345661692811No Hit
GAAACGGGGACAGTCCCTATTTCCTTTGCTGTGTTCCCCAATGAAGATGGCT730.3103345661692811No Hit
AACACAGGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAGTGCCACAGA720.30608340772860604No Hit
TGTGCTGGGAATCTACTGTTTGTGAATGTCTGTATTATCAGGAAACATGTCT710.30183224928793095No Hit
GCATATGGGAATTGAAATGAGATGAGTTGAAATGATGAGATGTAATGGTGAA710.30183224928793095No Hit
GTCGCCTGGGAGAGGCCATGGGCACGGCAGGCGGGCCTGGCCACCCTGTACA690.2933299324065808No Hit
ATCGCTGGGATTGTAAATTTTGAGATTTTATTTAGAAATGAATTGTCTAATT680.2890787739659057No Hit
ACTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC660.28057645708455553No Hit
TTTTAGGGGATACTAAAATGCTGCGTTAATTTTAGGCCATTGAAAGCTGTAC650.27632529864388045No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG640.27207414020320536No Hit
TTTGCCGGGATTTCATCTTGGGAAGTGCCCCAATCCTCCGGTGCAGGAGAAT630.2678229817625303No Hit
AAGTCTGGGCACAGCATGCATAAATTGCACATTTTGTTTTACTTTCTTTATA620.2635718233218552No Hit
AGAGTTGGGATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC610.25932066488118016No Hit
ATTTTCGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC610.25932066488118016No Hit
TGTGGCGGGATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT610.25932066488118016No Hit
TTTGCAGGGAGGCTTTGTTGGTCATACAGTCTCTGTCACAACTAATTAACTC600.255069506440505No Hit
AATTCAGGGAAATTAAAAATATGTGGGGGAGAGATCAGTGAAAAGTGGTTTT590.25081834799982994No Hit
CGCGCAGGGAAACCTTTGAGTCTAATAAAGCTTTCTGCAACTGAGGAACTTC570.24231603111847977No Hit
AACCATGGGACCTCTGTAAACTATTCCTAAAATGTGTATGATATATTTGAAA570.24231603111847977No Hit
CAGCCAGGGAGAATAACTTATTTCAGAAATGAGGAAACTGAGACTTATGATC570.24231603111847977No Hit
TTTTGCGGGCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTA560.2380648726778047No Hit
GGCTTAGGGAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTC560.2380648726778047No Hit
CAATAGGGGATGGGAATAAAGTTATTTCAATAGTTGACTCTTTTGAAAAGTT560.2380648726778047No Hit
TCATCCGGGAGGGATGGAGATGGGTCAGGCCTGGGCTTTGCCTGGGGAAGCC560.2380648726778047No Hit
TGACAAGGGACCTGACATTATCTCAGTTTTCTCCTATATTTTAGGAATTTTA560.2380648726778047No Hit
TGCTGGGGGCAAATTTAGTTTTCACAGCATGGTTTTGTTGTTTTCCCCTTGT550.23381371423712963No Hit
AGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTC550.23381371423712963No Hit
GAAGCCGGGGGGAGAGGGCTACCATGGGGGTGAGGGGAGGCCGCCCGCCGCG540.22956255579645451No Hit
TCTTGCGGGCATACAGACAAGGGGAAAAAGACGGCCTGGTTTTAAACCCCAG540.22956255579645451No Hit
TAAAATGGGATTAATACTAGGTTTTACATTTACCTATGTAGTTACCTTATCA530.22531139735577943No Hit
ATTTGGGATTGGATGCTAAAGCTGATATGAGCCTCCAAGTGCTCAGAGGCTG520.22106023891510437No Hit
ACTTCTGGGACTGCAACTAACTGTTGCCCCCCAACCCCGAACCCCCAGTTGA520.22106023891510437No Hit
TATGTTGGGACCCTTAAGACTGACATTTTCTCGTTAGGGAAAGAGACTCGAT510.21680908047442932No Hit
AGCGATGGGATTGCCGAGACTGGTCTCAAACTCCTGGAGTCAAGTGATCCTC510.21680908047442932No Hit
TGTGTCTGGGCTGTGGTGACAGTTTGTGACATTCTGTCTTCATGAGGTCTCA510.21680908047442932No Hit
TTGCCAGGGATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGT500.21255792203375418No Hit
TGACAAGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC500.21255792203375418No Hit
AGTTATGGGATCTTGCCTTTTTTCAGGCTAGCTGCAATAATTTTTTTCTTCT490.20830676359307912No Hit
GTTAAAGGATTTAAGGTAAATTATAAAACCTAAAATTAGTATCAGTATTTTA490.20830676359307912No Hit
GTCTTAGGGCAAAACTGTAAGCAGGATTGTCTGTAAGAATCACATTTCTATC490.20830676359307912No Hit
GCCCGGGGGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA480.20405560515240403No Hit
GTCTCTGGGGGTAACAACTGAAAGGGAAAAGTGCTTCAGCCATCTTTTGAAA470.19980444671172892No Hit
ATACTTGGGAAGACAGCCGTCTGTGACATCCCACCTCGTGGCCTCAAGATGG470.19980444671172892No Hit
ATCTTGGGGGAGGCGAGAGCTGCGAAAGGCGAGAGCTGCGAAGGGCCAGGTG470.19980444671172892No Hit
ATAATAGGGATCTCATCCTTTATTTTTAATCACTTTTTTTCTATGATGGATA460.19555328827105387No Hit
TTCTTAGGGAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC450.19130212983037878No Hit
TAAGCAGGGAGCTCATCTGTCAATTCTGTCAACTACTCAGGATCCTCTCAAG450.19130212983037878No Hit
TTAGCTGGGATACATGGTACACAGAGAGGGGTGTCACTTCAGAAAATCTTCC440.1870509713897037No Hit
GTTAGGGGGGGCAGATCACTTGAGCTTGGGGGTTTGAGACCAGCCTGGGCAA440.1870509713897037No Hit
GAACGTGGGAACTCACAGATGTTTCAGTTCATATTAGAATAAATTGCCCAAT440.1870509713897037No Hit
AAGATAGGGAAGCCTGTGGGAGTAGAAAAATCTCATTCCCAGAGTCAGAGGA430.1827998129490286No Hit
ATAGCAGGAGTAATGGTGCAGCATTGCCTGATGGAAATATAGCTGAGAGCTT430.1827998129490286No Hit
CTGATCGGGGTGCATAACCAGTCATGAGATAAAACAACACAATGCATGTTGC420.17854865450835353No Hit
GGATGTGGGACCTATGGAAAAAATCAATAATCTTTGGGATCTAATTTCTACA420.17854865450835353No Hit
GTGATGGGCGATCTCAAAATGGGAGAGGTAACAGATGCTCAGGACAGCTGCA420.17854865450835353No Hit
TTATTGGGACTCTGGCAAGACTGAAGATGAGGTACTTTTGTTTGTTTGGGTG420.17854865450835353No Hit
GCTTATGGGCTGATGCTGAACTGACACCAGGCGAATGTCAGGGCTCCCAAAC420.17854865450835353No Hit
AAGCGTGGGCTGATATAAATTTGGTGGCTGATGATTCAATTTAATGTAACAA390.16579517918632827No Hit
ATTCGGGGAGAACATTTGTTAAAACACAGATCAGAAACAGAGTTTCTGACTC390.16579517918632827No Hit
AAAAGTGGGCACTAGACTTGGCTTATACAAAATGCTAAAGGGAGTCCTTCAA390.16579517918632827No Hit
TCTATCGGGAGGTGGGGATAAAATCTCATACATGGATTTTTTCGTGTCCGCT380.1615440207456532No Hit
CCCGCGGTACTACAAGAACATCGGTCTGGGCTTCAAGACACCCAAGGAGGCT380.1615440207456532No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGA370.1572928623049781No Hit
GGTCTTGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT370.1572928623049781No Hit
TTATGTGGGCATAGCTTATTTGTCAAAGCAGCACACCTGTGAGGATTCCTGT370.1572928623049781No Hit
TAGAGGGGCCTAGGAAGTTACTATGGGAAAAGTGGAAATAGTTGAGGCCCAG370.1572928623049781No Hit
AGCGCGGGAAATTTGAATATTTTAATCCGTTTATATACTATTTAATATTGCT370.1572928623049781No Hit
GGTGCAGGGGAGCGCCTGTTAAAGCAACAGTGTAAGATTCTTTATATTGCAT370.1572928623049781No Hit
TTTGCGGAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACAT360.15304170386430302No Hit
CTTTATGGGATGGATGGTAGGAGGCACCCGGGTCTAAGGTGAGACTCTTTGT360.15304170386430302No Hit
CGTATGGGGGAATATTTAGGATTTGGTGAAGTTCACTGTATTGCTATATTTT350.14879054542362793No Hit
CACGGAGGGAATGGGTGGACTCACACTTGCTCAGTTCAGGCAGCTCTGCTGG350.14879054542362793No Hit
ACTTACGGGAGGATCAAAATTAATTTCAAACCTTCCAACCAACCCTATAAGC350.14879054542362793No Hit
ACCATAGGGAGTCAGTGTCCGGCCAACTCTGCAGCTCGGTCCAGCCCTGCCC350.14879054542362793No Hit
ATTTGCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA340.14453938698295285No Hit
GTTACAGGGACCTGTCTATTATGAAGGATATTATGCCAAATACAGATGCAGA340.14453938698295285No Hit
TTACATGGGAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACC340.14453938698295285No Hit
TCAGCCGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG340.14453938698295285No Hit
TGCTATGGGAATAATGGAGTAGCTGTTGCCCTTCTCCGGTTGTGTGTACAGT330.14028822854227777No Hit
TCAGCAGAGTTATTGAATCTTAATTTTTTTAATGTACAAGTTTTGTATAAAT330.14028822854227777No Hit
TCAACTGGGATGTTAAACACCAGTAGAGAAACAACTATGGTTGTGATTAAAT330.14028822854227777No Hit
TCTAGCGGGAGATCATGGATATAAAGAGATACCTGATTTTTATTAAAAAGAT330.14028822854227777No Hit
CGAAGGGACCTTACAAATTTCAAAGAATACAAACCATATAAAGTGTGCTCTC330.14028822854227777No Hit
GTAGGCGGGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGA320.13603707010160268No Hit
TTCGCAGGGCACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGG320.13603707010160268No Hit
TATACGGGGTGACTATAAAAGATTGGGGGGAAATAATTAAACTACTCTAAGC320.13603707010160268No Hit
ATGCACGGGAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC320.13603707010160268No Hit
ATTCCCGGGTTCAGGCAGTTCTCCTGGCTCAGCCTCCCAAGTGGCTGGGATC310.1317859116609276No Hit
CTTCACGGGAAGTGACACTACAGAAAAACACAAAAAGGTGATGGGTTGTGTT310.1317859116609276No Hit
TTCTTAGGGAAAGTTTAATTTCTTACACTGTGGTTGTCAAGAATACTGATTT310.1317859116609276No Hit
ACCATGGGCACCTTGTCCTCTCAAAATGCTTACAGGCATGAACCACTGTGAC310.1317859116609276No Hit
GGCTGCTCGGGACAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCA310.1317859116609276No Hit
GTTAGTGGGAATCCCAGTACTCTGGGAGGCCGAGGTGGGAGGATCACCTGAG310.1317859116609276No Hit
GATATGGGGATGCACATGTTGCAACAAGAGCTTCTGGGAAGGTAAGCGGCAT300.1275347532202525No Hit
GCCCTTGGGACTGAAAGAGGGCAGATATTGGGTCCTTGTAACTTCAACTGAC300.1275347532202525No Hit
TCTTTAGGGCATTTAAAATTTATATAAAACAACCTTCCATAAAAATTTGACA300.1275347532202525No Hit
AATAGTGGGGGGGTGGGGAACATGTCTGAGTCGGAGCTCGGCAGGAAGTGGG300.1275347532202525No Hit
TCTTGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG300.1275347532202525No Hit
TTCAAAGGGAGTTCACTGCCATGTTCCGCCGGAAGGCCTTCCTCCACTGGTA300.1275347532202525No Hit
ACCCAAGGGATGACTAATCATCAGGGTTTTAGTCTAATTAAAACCACAGTGA300.1275347532202525No Hit
TCACACGGGGAGGAAAACTAAATTCTCAAATAATTCATAATGGGCGAAGTGC300.1275347532202525No Hit
AGACAAGGGATGTGGCCTCTCCTTGAAAATATTGCATGTGCTTATGAAAAAA300.1275347532202525No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGT290.12328359477957744No Hit
CTTAATCGGGGTCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATA290.12328359477957744No Hit
TGGTACCGGGATTGTATATTTTATGTTTGTAAATTGTTTAGAAAGATAATTG290.12328359477957744No Hit
TTTGGCGGGATCTTCATCATGGACGAGGCCACGGCTTCCATTGACATGGCCA280.11903243633890236No Hit
AGTGAGGGGAAATTGATAACTTGCCTCTTTATCTTTGGCAACTCTCCCATGT280.11903243633890236No Hit
AGTTCTGGGAGTGTGGGTCCACAGGGCATAGGAACTTATGTTTTTGTTCCTA270.11478127789822726No Hit
GCTACTGGGACTGCATGACACATGGCCTGGTTACCTGTCATGGAATGCTGCA270.11478127789822726No Hit
AGAAATGGGAAGAAGTGACTGTTGTTAAGATAGACATTGAAGGACAGCAAAA270.11478127789822726No Hit
AACATAGGGATAGGATGATGTTTTTAAAGAAGTGTTAAAACAATTTAAATAA270.11478127789822726No Hit
GCTGTAGGGAGATTACTAAGTCATTTGTATGAATATGTGTGGCAGTGAAGAG270.11478127789822726No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGAACTCGTATGC270.11478127789822726No Hit
CAATCAGGGAGAGTAGGGAGCTGATAACAGTCCCAAGCCCTCCTCTTTCTCT270.11478127789822726No Hit
GAATCAGGGATAACTAAGCAGCACAATAACCGACTTAGCGAATTCAGGTAGA260.11053011945755219No Hit
TACTGTGGGAACAGTGGACTGCATATATGATGGTGGTCTCATAAGATTATAA260.11053011945755219No Hit
GCACTGGGATAGATGAGATAGGCTTTCATGTGCTTCTATGTTTTGCACATGA260.11053011945755219No Hit
AATCAGGGGAGCTCATGGATTCAGAAGTCTCAGTTCCATAGAGCATTTTGCT260.11053011945755219No Hit
GTAGCCGGGATTATAATTTAGTTCATACTGAAAAAGAAATTAACAGAACATT250.10627896101687709No Hit
CTAAACAAGGGATTTGGAAATTAGCTAGTGTAGTTTAAGATATCAGTATAAT250.10627896101687709No Hit
CCAGATGGGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACA250.10627896101687709No Hit
TCTAGAGGGATAAAATGAAAAGCTATACTACTTTTTATAAAAGGGCAAACTG250.10627896101687709No Hit
AGATCAGGGAAAATTGTCAACTTGAAACTAAAACATGCCAAGGTTTTGATAT240.10202780257620202No Hit
TATTCAGGGCGATCTTATATGGGGACTTTTTATGATACTCAGAAATTAGGAT240.10202780257620202No Hit
TCCTAGGGGATTATACCAGGATAGGGAAATTTTTCAAGGATGATTTAGGAAA240.10202780257620202No Hit
CTGTGAAGGGAGAAGATAAAAACTGCCCATTGTAACTTATTTCAGGTTAAAT240.10202780257620202No Hit
GGCACAGGGAGTGGGAGAACACAGAGACTAACTGGCATAAGGAAAAGATGGA240.10202780257620202No Hit
ATAAAAGGGTAAGTGGACCAGCATATTAGCATAGAGAAATCTCGAAATAAAT240.10202780257620202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)