FastQCFastQC Report
Sat 31 Dec 2022
SRR5844263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23406
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT3971.6961462872767668No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC3841.6406049730838246No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA1160.49559941895240534No Hit
AGAGCCGGGACCCCGTTTCTACCAAAAATACAAAAATTAGCCGGGCGTGGTG860.3674271554302316No Hit
AGGCACGGGATGGCCGCGGGCCGCCTCTTTCTAAGTCGGCTTCGAGCACCCT810.34606511150986924No Hit
ATTTTCGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC770.32897547637357943No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT760.32470306758950696No Hit
CGTTACGGGGGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGG750.3204306588054345No Hit
TGACAAGGGACCTGACATTATCTCAGTTTTCTCCTATATTTTAGGAATTTTA750.3204306588054345No Hit
AACCATGGGACCTCTGTAAACTATTCCTAAAATGTGTATGATATATTTGAAA710.30334102366914467No Hit
TTTGCAGGGAGGCTTTGTTGGTCATACAGTCTCTGTCACAACTAATTAACTC710.30334102366914467No Hit
GAAACGGGGACAGTCCCTATTTCCTTTGCTGTGTTCCCCAATGAAGATGGCT700.2990686148850722No Hit
ATGCGAGGGACAAGGAATATAGGCAGATGTACAGAACTGAAATTCGAATGGC680.2905237973169273No Hit
GGAACTGGGGATGGACCAGGAGCCAGTGGGCGGTGTGGAACGAGGAGAAGCC660.2819789797487824No Hit
TGCTGGGGGCAAATTTAGTTTTCACAGCATGGTTTTGTTGTTTTCCCCTTGT660.2819789797487824No Hit
GTTAAAGGATTTAAGGTAAATTATAAAACCTAAAATTAGTATCAGTATTTTA650.2777065709647099No Hit
TTTGCCGGGATTTCATCTTGGGAAGTGCCCCAATCCTCCGGTGCAGGAGAAT640.27343416218063743No Hit
TATTGGGGGAGCTAGGAATACCACTGATTTAGAAACAAAACTGTTTGTCTCT640.27343416218063743No Hit
AAGTCTGGGCACAGCATGCATAAATTGCACATTTTGTTTTACTTTCTTTATA630.269161753396565No Hit
TTGCCAGGGATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGT630.269161753396565No Hit
TAAAATGGGATTAATACTAGGTTTTACATTTACCTATGTAGTTACCTTATCA620.2648893446124925No Hit
GCCCGGGGGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA620.2648893446124925No Hit
GCATATGGGAATTGAAATGAGATGAGTTGAAATGATGAGATGTAATGGTGAA610.26061693582842005No Hit
TTTTGCGGGCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTA600.25634452704434757No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG600.25634452704434757No Hit
TCATCCGGGAGGGATGGAGATGGGTCAGGCCTGGGCTTTGCCTGGGGAAGCC600.25634452704434757No Hit
CAATAGGGGATGGGAATAAAGTTATTTCAATAGTTGACTCTTTTGAAAAGTT590.25207211826027515No Hit
CAGTATGGGATTTAAGCACTTAGGCAGAGTAGACACAATCAAAGGTACAAAG590.25207211826027515No Hit
ACTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC590.25207211826027515No Hit
ATACAGGGAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAA590.25207211826027515No Hit
AACACAGGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAGTGCCACAGA590.25207211826027515No Hit
TTAGCTGGGATACATGGTACACAGAGAGGGGTGTCACTTCAGAAAATCTTCC580.24779970947620267No Hit
TATGTTGGGACCCTTAAGACTGACATTTTCTCGTTAGGGAAAGAGACTCGAT580.24779970947620267No Hit
ACTTACGGGAGGATCAAAATTAATTTCAAACCTTCCAACCAACCCTATAAGC580.24779970947620267No Hit
AGCTAGGGAGACCTAGTTCTATTATCATTTGTTAGTTCACTGTGCTCAAGTC580.24779970947620267No Hit
GAAGCCGGGGGGAGAGGGCTACCATGGGGGTGAGGGGAGGCCGCCCGCCGCG560.23925489190805777No Hit
TTTTAGGGGATACTAAAATGCTGCGTTAATTTTAGGCCATTGAAAGCTGTAC550.2349824831239853No Hit
TGTGTCTGGGCTGTGGTGACAGTTTGTGACATTCTGTCTTCATGAGGTCTCA550.2349824831239853No Hit
GTCTCTGGGGGTAACAACTGAAAGGGAAAAGTGCTTCAGCCATCTTTTGAAA540.23071007433991286No Hit
CAGCCAGGGAGAATAACTTATTTCAGAAATGAGGAAACTGAGACTTATGATC540.23071007433991286No Hit
GCTTATGGGCTGATGCTGAACTGACACCAGGCGAATGTCAGGGCTCCCAAAC540.23071007433991286No Hit
AGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTC530.22643766555584038No Hit
ATAATAGGGATCTCATCCTTTATTTTTAATCACTTTTTTTCTATGATGGATA520.2221652567717679No Hit
TGTGGCGGGATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT520.2221652567717679No Hit
TCTTGCGGGCATACAGACAAGGGGAAAAAGACGGCCTGGTTTTAAACCCCAG520.2221652567717679No Hit
CGCGCAGGGAAACCTTTGAGTCTAATAAAGCTTTCTGCAACTGAGGAACTTC510.21789284798769548No Hit
GTCGCCTGGGAGAGGCCATGGGCACGGCAGGCGGGCCTGGCCACCCTGTACA510.21789284798769548No Hit
TGTGCTGGGAATCTACTGTTTGTGAATGTCTGTATTATCAGGAAACATGTCT500.213620439203623No Hit
TGCTATGGGAATAATGGAGTAGCTGTTGCCCTTCTCCGGTTGTGTGTACAGT490.20934803041955055No Hit
ATTTGGGATTGGATGCTAAAGCTGATATGAGCCTCCAAGTGCTCAGAGGCTG490.20934803041955055No Hit
TGACAAGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC490.20934803041955055No Hit
ATCTTGGGGGAGGCGAGAGCTGCGAAAGGCGAGAGCTGCGAAGGGCCAGGTG490.20934803041955055No Hit
AGCGATGGGATTGCCGAGACTGGTCTCAAACTCCTGGAGTCAAGTGATCCTC480.20507562163547807No Hit
ATTCGGGGAGAACATTTGTTAAAACACAGATCAGAAACAGAGTTTCTGACTC480.20507562163547807No Hit
ATAGCAGGAGTAATGGTGCAGCATTGCCTGATGGAAATATAGCTGAGAGCTT470.2008032128514056No Hit
AAAAGTGGGCACTAGACTTGGCTTATACAAAATGCTAAAGGGAGTCCTTCAA470.2008032128514056No Hit
ATACTTGGGAAGACAGCCGTCTGTGACATCCCACCTCGTGGCCTCAAGATGG460.19653080406733317No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGA450.1922583952832607No Hit
AGAGTTGGGATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC450.1922583952832607No Hit
GTTAGGGGGGGCAGATCACTTGAGCTTGGGGGTTTGAGACCAGCCTGGGCAA450.1922583952832607No Hit
GAACGTGGGAACTCACAGATGTTTCAGTTCATATTAGAATAAATTGCCCAAT450.1922583952832607No Hit
TAAGCAGGGAGCTCATCTGTCAATTCTGTCAACTACTCAGGATCCTCTCAAG450.1922583952832607No Hit
AGTTCTGGGAGTGTGGGTCCACAGGGCATAGGAACTTATGTTTTTGTTCCTA440.18798598649918824No Hit
GTCTTAGGGCAAAACTGTAAGCAGGATTGTCTGTAAGAATCACATTTCTATC440.18798598649918824No Hit
AATTCAGGGAAATTAAAAATATGTGGGGGAGAGATCAGTGAAAAGTGGTTTT440.18798598649918824No Hit
ACTTCTGGGACTGCAACTAACTGTTGCCCCCCAACCCCGAACCCCCAGTTGA440.18798598649918824No Hit
ATCGCTGGGATTGTAAATTTTGAGATTTTATTTAGAAATGAATTGTCTAATT420.17944116893104334No Hit
GGCTTAGGGAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTC420.17944116893104334No Hit
CTAAACAAGGGATTTGGAAATTAGCTAGTGTAGTTTAAGATATCAGTATAAT420.17944116893104334No Hit
CAATCAGGGAGAGTAGGGAGCTGATAACAGTCCCAAGCCCTCCTCTTTCTCT420.17944116893104334No Hit
TCTATCGGGAGGTGGGGATAAAATCTCATACATGGATTTTTTCGTGTCCGCT410.17516876014697086No Hit
TTCTTAGGGAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC410.17516876014697086No Hit
GGTCTTGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT400.17089635136289838No Hit
TCTTCCATAGGGCTCCTGCTTCAGCCTGTCGAGTAGCTGGGATTATAGGCAT400.17089635136289838No Hit
CTTTATGGGATGGATGGTAGGAGGCACCCGGGTCTAAGGTGAGACTCTTTGT400.17089635136289838No Hit
ATTTGCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA390.16662394257882593No Hit
TTATGTGGGCATAGCTTATTTGTCAAAGCAGCACACCTGTGAGGATTCCTGT390.16662394257882593No Hit
TCAGCAGAGTTATTGAATCTTAATTTTTTTAATGTACAAGTTTTGTATAAAT380.16235153379475348No Hit
AGCGCGGGAAATTTGAATATTTTAATCCGTTTATATACTATTTAATATTGCT380.16235153379475348No Hit
ATGCACGGGAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC380.16235153379475348No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGT370.15807912501068103No Hit
AAGATAGGGAAGCCTGTGGGAGTAGAAAAATCTCATTCCCAGAGTCAGAGGA370.15807912501068103No Hit
TTCAAAGGGAGTTCACTGCCATGTTCCGCCGGAAGGCCTTCCTCCACTGGTA360.15380671622660858No Hit
TTCGCAGGGCACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGG360.15380671622660858No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGAACTCGTATGC360.15380671622660858No Hit
GGTGCAGGGGAGCGCCTGTTAAAGCAACAGTGTAAGATTCTTTATATTGCAT360.15380671622660858No Hit
TGCCTCGGGAAGCTATTGCATGATTAGCCCCCTATCTGGCAAGGAAACCCCA350.1495343074425361No Hit
CGTATGGGGGAATATTTAGGATTTGGTGAAGTTCACTGTATTGCTATATTTT350.1495343074425361No Hit
TCAACTGGGATGTTAAACACCAGTAGAGAAACAACTATGGTTGTGATTAAAT350.1495343074425361No Hit
TACTGTGGGAACAGTGGACTGCATATATGATGGTGGTCTCATAAGATTATAA350.1495343074425361No Hit
TCAGCCGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG350.1495343074425361No Hit
TTATTGGGACTCTGGCAAGACTGAAGATGAGGTACTTTTGTTTGTTTGGGTG350.1495343074425361No Hit
GAATCAGGGATAACTAAGCAGCACAATAACCGACTTAGCGAATTCAGGTAGA340.14526189865846365No Hit
GCCCTTGGGACTGAAAGAGGGCAGATATTGGGTCCTTGTAACTTCAACTGAC340.14526189865846365No Hit
TAGAGGGGCCTAGGAAGTTACTATGGGAAAAGTGGAAATAGTTGAGGCCCAG340.14526189865846365No Hit
TTCTTAGGGAAAGTTTAATTTCTTACACTGTGGTTGTCAAGAATACTGATTT340.14526189865846365No Hit
AATAGTGGGGGGGTGGGGAACATGTCTGAGTCGGAGCTCGGCAGGAAGTGGG330.1409894898743912No Hit
AGACAAGGGATGTGGCCTCTCCTTGAAAATATTGCATGTGCTTATGAAAAAA330.1409894898743912No Hit
GTAGCCGGGATTATAATTTAGTTCATACTGAAAAAGAAATTAACAGAACATT320.13671708109031872No Hit
GTCAACGGGATTGCTTGGATTTCTAGTGAGAGCTGTTGAATTTGGTGATGTC320.13671708109031872No Hit
GCTACTGGGACTGCATGACACATGGCCTGGTTACCTGTCATGGAATGCTGCA320.13671708109031872No Hit
TCTTTAGGGCATTTAAAATTTATATAAAACAACCTTCCATAAAAATTTGACA320.13671708109031872No Hit
CACGGAGGGAATGGGTGGACTCACACTTGCTCAGTTCAGGCAGCTCTGCTGG320.13671708109031872No Hit
GTAGGCGGGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGA320.13671708109031872No Hit
CCAGATGGGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACA320.13671708109031872No Hit
CGAAGGGACCTTACAAATTTCAAAGAATACAAACCATATAAAGTGTGCTCTC320.13671708109031872No Hit
ATTCCCGGGTTCAGGCAGTTCTCCTGGCTCAGCCTCCCAAGTGGCTGGGATC310.13244467230624626No Hit
GGATGTGGGACCTATGGAAAAAATCAATAATCTTTGGGATCTAATTTCTACA310.13244467230624626No Hit
CTTCACGGGAAGTGACACTACAGAAAAACACAAAAAGGTGATGGGTTGTGTT310.13244467230624626No Hit
AGTTATGGGATCTTGCCTTTTTTCAGGCTAGCTGCAATAATTTTTTTCTTCT300.12817226352217378No Hit
TAAGGTGGGATTATTCATTTTGATGCACAAATTGTTTCAAATTTAACAAGAG300.12817226352217378No Hit
AAGCGTGGGCTGATATAAATTTGGTGGCTGATGATTCAATTTAATGTAACAA300.12817226352217378No Hit
GTGATGGGCGATCTCAAAATGGGAGAGGTAACAGATGCTCAGGACAGCTGCA300.12817226352217378No Hit
ATAAAAGGGTAAGTGGACCAGCATATTAGCATAGAGAAATCTCGAAATAAAT300.12817226352217378No Hit
GTTAGTGGGAATCCCAGTACTCTGGGAGGCCGAGGTGGGAGGATCACCTGAG300.12817226352217378No Hit
GATATGGGGATGCACATGTTGCAACAAGAGCTTCTGGGAAGGTAAGCGGCAT290.12389985473810133No Hit
TGCAAGGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT290.12389985473810133No Hit
CCCGCGGTACTACAAGAACATCGGTCTGGGCTTCAAGACACCCAAGGAGGCT290.12389985473810133No Hit
TCTAGAGGGATAAAATGAAAAGCTATACTACTTTTTATAAAAGGGCAAACTG290.12389985473810133No Hit
CGGTTAGGGCACTTAATTTTTTACACAAACGCCCAACTATTTTCCTGAGAGT280.11962744595402888No Hit
GTTACAGGGACCTGTCTATTATGAAGGATATTATGCCAAATACAGATGCAGA280.11962744595402888No Hit
GGCTTCGGGGAAGAAAACTTCCAGTTAAAAAAAATTGGATAATGTTTTTGAT280.11962744595402888No Hit
AGAAATGGGAAGAAGTGACTGTTGTTAAGATAGACATTGAAGGACAGCAAAA280.11962744595402888No Hit
TCTTGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG280.11962744595402888No Hit
AACATAGGGATAGGATGATGTTTTTAAAGAAGTGTTAAAACAATTTAAATAA280.11962744595402888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCCTGTCTCTTATAC280.11962744595402888No Hit
GCACTGGGATAGATGAGATAGGCTTTCATGTGCTTCTATGTTTTGCACATGA280.11962744595402888No Hit
TTACATGGGAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACC270.11535503716995643No Hit
TCCTAGGGGATTATACCAGGATAGGGAAATTTTTCAAGGATGATTTAGGAAA270.11535503716995643No Hit
TCTAGCGGGAGATCATGGATATAAAGAGATACCTGATTTTTATTAAAAAGAT270.11535503716995643No Hit
TATAAAGGGATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTC270.11535503716995643No Hit
ACCATAGGGAGTCAGTGTCCGGCCAACTCTGCAGCTCGGTCCAGCCCTGCCC270.11535503716995643No Hit
CATTATGGGAGGAAGCTCTCAGGGAAATGTCTGTGAATCATTTAAAGATGCT260.11108262838588395No Hit
GTGATCGGGGATCTTACCAAGGGAAGGACATCAAGAAGATCTTGGACAGCGT260.11108262838588395No Hit
AGTGAGGGGAAATTGATAACTTGCCTCTTTATCTTTGGCAACTCTCCCATGT260.11108262838588395No Hit
ACCCAAGGGATGACTAATCATCAGGGTTTTAGTCTAATTAAAACCACAGTGA260.11108262838588395No Hit
GAAGCCGGGATTGTTTAAAGGACAACTACAAATCATGCTGGATAAGAGTACT260.11108262838588395No Hit
GTCAATGGGTGCCAAATTTGTACTTTGGAATCCTCCTCTGCATGTTCATAAG250.1068102196018115No Hit
CTTAATCGGGGTCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATA250.1068102196018115No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGAT250.1068102196018115No Hit
TTGCTCAGGGAAGTATTATGTATATGCGAGATTCCAGGATTATTCTGTTAGT250.1068102196018115No Hit
AGATCAGGGAAAATTGTCAACTTGAAACTAAAACATGCCAAGGTTTTGATAT240.10253781081773904No Hit
CAAGGAGGGATTCTCACCCTATTAAATGGAAATTTAGAGCACCCTTAAATTC240.10253781081773904No Hit
CTCTCATTGGGAAAGTAACCTAAAATGAATTCAGACCAATAAAATCAAGGGA240.10253781081773904No Hit
AATGTAGGGAGAGAGGAAACGTCACTGAGATGAAGAGGCGGGTAAATTGGTT240.10253781081773904No Hit
GCTGTAGGGAGATTACTAAGTCATTTGTATGAATATGTGTGGCAGTGAAGAG240.10253781081773904No Hit
GGCTGCTCGGGACAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCA240.10253781081773904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)