FastQCFastQC Report
Sat 31 Dec 2022
SRR5844261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123494
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT6070.49152185531280873No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC5300.42917064796670285No Hit
AAACTTGGGGAACTCCTGACCTCAAGTGATCCACTCACCTCGGCCTCCCAAA1720.13927802160428848No Hit
TATTAAGGGTGTTATTTGATTTCAGGTTTCACAACAAACCAAAACTAAAAGT1690.136848753785609No Hit
TTTCACGGGTATCAGTTGATTGTTATCTCTCGGAAAAATGTACCTTTGGTTA1640.13279997408780994No Hit
TGTGCGGGTATCAGTTCAAACGAGACTTACTACACTGGGCTATTTCTTTTTT1560.1263219265713314No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA1550.12551217063177159No Hit
CAATTTGGGCAGATTATCCATTTAAGCCACCAAAGGTTACTTTCCGCACCAG1540.12470241469221177No Hit
GAAGTTGGGATATTGAAACATTCTATCCAGTAGCTACAGAATACCCATTCTT1520.12308290281309213No Hit
TTGACTGGGGGCGAGCCCAGCCGCAGCAGCCGGGCACCAACGCTTGAGTGCA1510.12227314687353233No Hit
GCTGCAGGGCAACTTCACCAGGTTTCTAAATGTGTTTTAGAACCAAAATACT1490.12065363499441269No Hit
AAGTTCGGGCAGATGAATCAAAATTCAATATGTACCGGCTCTACTTTGGAAG1480.11984387905485286No Hit
ACAATGGGGAAGAATTTGGAGTTTTTCAGGGCAATTCAAATGACCTCATTTT1460.11822436717573324No Hit
GATTTTGGGGGAGGCGGTCCTCCTAGCGGCCTGGTAGTGTTTTTGTTGCCTT1450.11741461123617342No Hit
CAGCTAGGGGATGAAAAAAATTAGCAAACCGAAGTTTCTTCAATGACCCCTC1430.11579509935705379No Hit
AGCACGGGGATGATGAGCATGATAAAATGAATTATCTTACACATTAATTGAA1430.11579509935705379No Hit
GCGCAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1420.11498534341749396No Hit
TTCAAGGGTCAGCTTTTGACTTTGAATCTCATGAAAAAAGAAAATTTAAAAA1420.11498534341749396No Hit
AGGCTAGGGAGTTAAAGAGGAATGCAGAACAGGTTCTCAGAAGAGAGATGAG1420.11498534341749396No Hit
GGATTTAGTGAAATGCCGTATGCAGGTGGACCCCCAAAAGTACAAGGGCATA1420.11498534341749396No Hit
ATACTTGGGATCAACACTGGAAAAGAGACTTCACTATTTTCGAAATTATCCC1420.11498534341749396No Hit
CAATTAGGGATACCCTGACAACCTGGAAGATTGCTTCAGTCCTATTTCCTGT1420.11498534341749396No Hit
AACCATGGGGAGTGAGACAAACTTCTATGTGTTTGAACTACTACCTTTTGTG1420.11498534341749396No Hit
ATTAATAGGTGGTAAAAATCTATGTATAAATAAATCTAAAAGAGCCCTTTTT1410.11417558747793416No Hit
TATGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1410.11417558747793416No Hit
GCCGAGGCTGGAATTGCCGGGGAGGCCCAAAGCAAGAAGCCAGTGTCCAGGC1400.11336583153837433No Hit
GCAGTTGGGAGGGCCTGAACAAAAGTGAGACAAGTGAGGTGCACAGGGGTCC1400.11336583153837433No Hit
CTATGTGGGGCACAATTAAGTTTAAAAATAAAGAAGTGAAAGTTTCAGACTA1390.11255607559881452No Hit
GAAAATGGGATCATGGCAATATGCCAGTAACTTCTGAATGAGGGAAACCATC1380.11174631965925472No Hit
AAAAACGGAATGTTAAATCATACCCCTGTGAGAAACAGCTTTACCAAGTAAG1380.11174631965925472No Hit
CGCGTTGGGGGGGCTGTGCTGGTGGCGTTCAAGATGTCGACCAAGAATTTCC1380.11174631965925472No Hit
TAGAAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1380.11174631965925472No Hit
CGCCTAGGGCAAACTAGTGAATGAAGAACGAACGCTGGAAGTAGAAATAGAG1380.11174631965925472No Hit
ACAGCCGGGCGGTTATTATTATCATTTCTATTTTGTAGATGAGGAAGTTGAA1330.10769753996145563No Hit
GATACTGGGACTCAAAGAAGATGGTACACTGAAGAAAGAAAAAGTCAGGAAG1330.10769753996145563No Hit
TTAATCGGGCAGCCTGTGTGCTTCCAGGCTCCCCAGAGACAAGACGGCACCG1330.10769753996145563No Hit
ACTAGCGGGGACATTTTGACATTTGGAGAAGAAAGATGGCACACTAACCCAG1320.1068877840218958No Hit
CGTCACGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1310.10607802808233599No Hit
ATTCAAGGGGCATGCTGTGATGGTTGATATTAGGTGTCAACTTGACTGGTTG1310.10607802808233599No Hit
TGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACT1300.10526827214277616No Hit
CTCCATGGGCAATAGGGATATGAATTACATAAAAAGAATTTTGTGTAACTGG1300.10526827214277616No Hit
CATATGGGGCTGGCTGTTGTTTTATAATCAAATTTTATCTCAGATCAGCCAT1300.10526827214277616No Hit
CAAAAAGGGATAGAGGTAATATGGAAGTCATAAAAGTAAATTAAGGGAGAGA1300.10526827214277616No Hit
TGTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.10445851620321635No Hit
ATCCCAGGGATGAAAGGATGCTTCAGTATACATGACTCAATTAATGTGGTAT1290.10445851620321635No Hit
TGCCGAGGGTCTTCAGTACAAAGAGTATCCAAAAGCTAAGTTTTTGTATTCC1290.10445851620321635No Hit
AAAAAGGGGGTGATTATTGAAGACAGAGTGCTGTGTGAGACAACCTGCTTTA1280.10364876026365655No Hit
CTCACAGGGAAGTATATTTTGAATCTTTTTTTGATCATAAATTTTGAAATGG1280.10364876026365655No Hit
TTCAATGGGGGATGTGTCTGACTAAAAACATGAGGCTCTTACAACAAAAATG1280.10364876026365655No Hit
CTTAACGGGAGAACAGAAGTACAAATGTGAAAACTTACAAACAACTTAGTTT1280.10364876026365655No Hit
GTTTTTGGGAAGGGAGGAGGATGGGGGAAGGGTGGGGGCACCCACGACCTGG1280.10364876026365655No Hit
TTCACTGGGATCTGGGAGTGAACTTAACATTCACTGTGTCTCTGCTCTGCGT1280.10364876026365655No Hit
GTAAAAGGGAACCTCACAGAATGGACAGTGAACAGCCGGTGGGACACAGGCA1270.10283900432409672No Hit
CTCTTCGGGGCTATTACTTACAAAATTCATCAATTACTAGTCCATGAAAAAT1270.10283900432409672No Hit
TTCAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1270.10283900432409672No Hit
TATATAGGGCCCATTTTGTCAATTTACATATTTTCTATTTGAAGCACTTCCC1270.10283900432409672No Hit
AGCATGGGGAAGTCTCTCTTGATGACCATCATTTGCTTTTGATAATTTATTA1270.10283900432409672No Hit
TGTTCCGGGCAGTTGACGTTTTTCTGTTGAACTGATTTTTCTTTTTTCCAAT1270.10283900432409672No Hit
GCAACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.10121949244497708No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.10121949244497708No Hit
TCCGGCGGGAAAGTGTAGAATCTGTTGCTACATTAAGCTGTCGCTGCCCACA1250.10121949244497708No Hit
CAATAGGGACCAAGAGATTTTGAACTATCCAATTATGACTGCTATGGAAAAC1250.10121949244497708No Hit
TCTCCGGGGACACTTGAGAGCCAACACAACATATTTATTACTAAAAAGATGC1250.10121949244497708No Hit
ACGCAGGGGGACACATGGAAGTCATTCAGTGACTTTGGAATGGGTAGGTTCG1240.10040973650541726No Hit
GCAAATGGGGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAAGT1240.10040973650541726No Hit
TCTAGCGGGGGTGTGTTGCATTTGAGAGAAGGCAAGAAGAAAAGACAATAGA1240.10040973650541726No Hit
TATTCAGGTATCTTATTGGATGTATACAAAGTGATTGTGTGTCATTTGAACC1240.10040973650541726No Hit
GCTCAAGGGGGAAATAACAGGTACAGTGCTGTCTTCTAATGTTGAGACAGTA1240.10040973650541726No Hit
AGCTAAGGGATAATGAGTTTGTTCTAACAACAAAGAATTACATCCGGACATG1240.10040973650541726No Hit
TGAGCTGGGCAACAGAATATACATTCTTCTCAGCACCAGATCACACTTATTC1240.10040973650541726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)