FastQCFastQC Report
Sat 31 Dec 2022
SRR5844260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120846
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT5580.46174469986594513No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC5100.42202472568392835No Hit
TGTTCCGGGCAGTTGACGTTTTTCTGTTGAACTGATTTTTCTTTTTTCCAAT1710.14150240802343478No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA1600.13239991394005593No Hit
TTTCACGGGTATCAGTTGATTGTTATCTCTCGGAAAAATGTACCTTTGGTTA1560.12908991609155454No Hit
AAACTTGGGGAACTCCTGACCTCAAGTGATCCACTCACCTCGGCCTCCCAAA1530.1266074177051785No Hit
AGCACGGGGATGATGAGCATGATAAAATGAATTATCTTACACATTAATTGAA1510.1249524187809278No Hit
ATCTTAGGGCTCATTCAGAAATTTTCTACTGTGGTTTGTGGATTTCTCCAGG1500.12412491931880244No Hit
ACAATGGGGAAGAATTTGGAGTTTTTCAGGGCAATTCAAATGACCTCATTTT1500.12412491931880244No Hit
GAAGTTGGGATATTGAAACATTCTATCCAGTAGCTACAGAATACCCATTCTT1490.12329741985667708No Hit
CAGCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1480.12246992039455175No Hit
AGTGACGGGTGGCCCTTCCTTCTGCGCCGTTAACTAGGAAGTATAAAGTACT1480.12246992039455175No Hit
TATTAAGGGTGTTATTTGATTTCAGGTTTCACAACAAACCAAAACTAAAAGT1470.12164242093242639No Hit
CTCTTCGGGGCTATTACTTACAAAATTCATCAATTACTAGTCCATGAAAAAT1460.12081492147030105No Hit
CAATTTGGGCAGATTATCCATTTAAGCCACCAAAGGTTACTTTCCGCACCAG1440.11915992254605033No Hit
GCCGAGGCTGGAATTGCCGGGGAGGCCCAAAGCAAGAAGCCAGTGTCCAGGC1420.11750492362179964No Hit
TTCAAGGGTCAGCTTTTGACTTTGAATCTCATGAAAAAAGAAAATTTAAAAA1420.11750492362179964No Hit
CAATTAGGGATACCCTGACAACCTGGAAGATTGCTTCAGTCCTATTTCCTGT1420.11750492362179964No Hit
CTCATCGGGAGAGATGTAAGGCTGTCACTTTACAGGACAAGGGAGAGAAGAA1400.11584992469754894No Hit
GCTGCAGGGCAACTTCACCAGGTTTCTAAATGTGTTTTAGAACCAAAATACT1400.11584992469754894No Hit
TTAATCGGGCAGCCTGTGTGCTTCCAGGCTCCCCAGAGACAAGACGGCACCG1400.11584992469754894No Hit
ATTAATAGGTGGTAAAAATCTATGTATAAATAAATCTAAAAGAGCCCTTTTT1390.11502242523542361No Hit
CTTAACGGGAGAACAGAAGTACAAATGTGAAAACTTACAAACAACTTAGTTT1380.11419492577329825No Hit
CAAAAAGGGATAGAGGTAATATGGAAGTCATAAAAGTAAATTAAGGGAGAGA1380.11419492577329825No Hit
GTAGAGGGGGTACATTGAAGCTCGGGTGACAAAAGGTGAGACACTCACCTAG1380.11419492577329825No Hit
ACGCAGGGGGACACATGGAAGTCATTCAGTGACTTTGGAATGGGTAGGTTCG1370.11336742631117289No Hit
CAGCTAGGGGATGAAAAAAATTAGCAAACCGAAGTTTCTTCAATGACCCCTC1340.11088492792479686No Hit
ATTGCAGGGATTGGAATTTTGATAGGGATTGCACTAAATCTGTACTTTGGGT1340.11088492792479686No Hit
CTAGACGGGTGTTAACATTTTGAGGGTTTTGAATGGTGATTTGTTCCCAGTT1330.1100574284626715No Hit
ACAGCCGGGCGGTTATTATTATCATTTCTATTTTGTAGATGAGGAAGTTGAA1320.10922992900054614No Hit
GGCTTCCCATTTTTAGAATCAGAGAATGAAAAAAACGCACCTTGGAGAAAAA1320.10922992900054614No Hit
GCTATGGGGTAAATTCTCTGCCCTCTGCAAACAGCCATCTGTCTGAGAACTC1310.1084024295384208No Hit
GATACTGGGACTCAAAGAAGATGGTACACTGAAGAAAGAAAAAGTCAGGAAG1310.1084024295384208No Hit
CATACAGGGATTGTAGAATATACCATAAAGTCAGCAAGTGCTGCATTACTGG1300.10757493007629544No Hit
CGTCACGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1290.10674743061417011No Hit
ATTTACGGGAACATTCTGTGACCAATAATATTTTAGTTAACAGAGATTCTGG1290.10674743061417011No Hit
CGCCTAGGGCAAACTAGTGAATGAAGAACGAACGCTGGAAGTAGAAATAGAG1290.10674743061417011No Hit
AGGCTAGGGAGTTAAAGAGGAATGCAGAACAGGTTCTCAGAAGAGAGATGAG1280.10591993115204475No Hit
TCTGAGTGGGAATCTATTTCTGTAACCATGGAAAAACAGAAATCAACTAGAC1280.10591993115204475No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.10591993115204475No Hit
TTCGCCTGGGATCCTGTTAACAATAAATAGTCCCTTAGCAATTACTGTACTC1280.10591993115204475No Hit
CAAAAAAACAAACAAACAAACAAACAAAATAGATAAAAATTATCTTTTTTCC1280.10591993115204475No Hit
TGCTCGGGGGACCACGGATTTGCATTGCCGAGGACGGGACCCCAGGGCAGCG1270.10509243168991939No Hit
TCTGCAGGGCTGAATAGTTTAGTCCAGATTTTTACAGAGGATACATCTATTT1270.10509243168991939No Hit
GTGCTGGGATGCCAAAGCATCATCTTCTGGAGTATTGTCTTCTGAGAGTCAA1270.10509243168991939No Hit
GCAGTTGGGAGGGCCTGAACAAAAGTGAGACAAGTGAGGTGCACAGGGGTCC1270.10509243168991939No Hit
GGTACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10509243168991939No Hit
TGCGATGGGCTCTTGGTTTTGATTTAAGGTAATAGGGTCTGTCACTTTTTTT1270.10509243168991939No Hit
TTCACTGGGATCTGGGAGTGAACTTAACATTCACTGTGTCTCTGCTCTGCGT1270.10509243168991939No Hit
GAAACCGGGAGGAGCGTGAGTTGTGCTGTCCTGGCTCGAGAAGAAGGCAGCA1260.10426493222779405No Hit
CTCACAGGGAAGTATATTTTGAATCTTTTTTTGATCATAAATTTTGAAATGG1260.10426493222779405No Hit
TTAAAAGGGGTAAAATTTTTATAGTGAAACATTTTAATGACAATTTAAAAAT1260.10426493222779405No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT1260.10426493222779405No Hit
CATTGGGGGGCAACCTGGGCCTTGTACTGTCTGTGTTTTTAAAACCACTAAA1260.10426493222779405No Hit
GAAGTGGGGACATTTTAGTTCCATTTTATAAGGCTTCACCTTCTTCTCAACC1250.10343743276566869No Hit
AAAGCAGGGCTAGGGAAGGGAACCAAAAAAATAAACCCTCGTTCCACAGAAG1250.10343743276566869No Hit
TATTACGGGGGCATTGCAATGTGAGAATGACAGAGTTGACAGGACCACTAAC1250.10343743276566869No Hit
AAGCTGGGGGTCTCCAAGCCCACTTGAAAAGTCACACTGAAAGGATGCAAAT1250.10343743276566869No Hit
TGCCGAGGGTCTTCAGTACAAAGAGTATCCAAAAGCTAAGTTTTTGTATTCC1250.10343743276566869No Hit
ACCTGGGGATGAAGAGACCAATTAAGATTCATTCCTCATTCTGTTTCTTTCC1240.10260993330354336No Hit
CGCGTTGGGGGGGCTGTGCTGGTGGCGTTCAAGATGTCGACCAAGAATTTCC1230.101782433841418No Hit
GGCGGGGGGATGGGGTTTCGCACTTGTTGCCCAGGCTGGAGTGCAATGGTGC1230.101782433841418No Hit
GTAGCTGGGATAACTGTTTAAAAATATATATGCTTATAGTCAAAAGCTGTTG1230.101782433841418No Hit
TCCCACAGCTTCAATCACTGTCATTATTCTAATCCTACTCCTACTTAAATTT1230.101782433841418No Hit
GAATTTGGGGCTACCCAGGCGGTGGTGTCGATCTACGTTCCAATTGGGGCCG1230.101782433841418No Hit
ATACTTGGGATCAACACTGGAAAAGAGACTTCACTATTTTCGAAATTATCCC1230.101782433841418No Hit
AATGGTGGGTCAACATAGGGTGAAACTATTTCTGATAAAGAACTTTTGAAAA1230.101782433841418No Hit
ATTGCTGGGAATGCACATGTGAGCCACCGCGCCTGTCCTTGATTTTAGATCT1220.10095493437929266No Hit
GTCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1220.10095493437929266No Hit
TTGAGAGGGAAGAGCAGAGATATTGTGTTGTGTAAAATTAAGACTTAAAGAT1220.10095493437929266No Hit
GGGCATGGGACCATTCTATAGGAAATGAAAGGCAGAGAAGTAAAGTGATTTG1210.1001274349171673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)