FastQCFastQC Report
Sat 31 Dec 2022
SRR5844256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202703
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT10330.5096125859015407No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC7710.38035944213948486No Hit
ATGTTAGGGTCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAA3510.17315974603237247No Hit
GCTTGAGGGAGAAATCAAGATGGACATTTCCCTATGTTAAAAAAAGTCAGTT3180.1568797699096708No Hit
TACAGTGGGAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGGC2780.13714646551851722No Hit
TTACCAGGGGGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAAA2580.12727981332294047No Hit
GTTACAGGGCAGGATAAAAAACATTGTATTAAATTTCTGTCCAAGGAGAAGA2560.1262931481033828No Hit
TTAAATGGGCTGACGGAGGGAAACCAACAAAGATCAAAAGAGACAAAGAAGG2500.12333315244470976No Hit
GTTGCTGGGGGGTAGCCAAGGCTGTAGTGAGCCTTGATAGCGCCACTGCATT2410.1188931589567002No Hit
TAAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2400.11839982634692135No Hit
TACGATGGGAGTTCATTCAGTAAGCTTAGAGAGTACTCAGTCCAGATTGAAG2330.11494649807846948No Hit
ATAACCGGGCATCAGACAACAGGTAAGTTTTTTTTTAACTTATTGACCATGA2280.11247983502957529No Hit
CCGACTGGGGGCATTTTAATGTATTTTAAAATAAATTTCAAAATTCTGCTAA2240.11050650459045992No Hit
TAAATGGGGCTTTGCCTTTTCTGTACACCTGTGAGACCAACAGATTGTCTAA2200.10853317415134459No Hit
CGGGATGGGATCCACAGTCAGCAATTTTATTGAAAATCAAATGAAAAGGAAT2170.10705317632200807No Hit
CAGTCGGGAAGTGAGCATCTGGCGGTAGTAGCATTTTTAGATATATTCTTGG2120.10458651327311386No Hit
ACCTCCGGGAGTCTCTAAGATACAACACTCTGTTACTTTTCAAGGTTCAACA2100.10359984805355618No Hit
TCTGGTGGGGAAGAAAATATTCACAAACTATGGGTATAGCAAATGCATATTA2090.10310651544377734No Hit
GCATGGGGAACTGCCTTCTAAACAAGGTTACTATTTACAGGGAAATTATCTG2080.10261318283399852No Hit
GCTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2060.10162651761444082No Hit
CTTTGCGGGGAGTGGCAATGCAGGAATCTATGGTAATGGCTAATATACCATG2050.101133185004662No Hit
CAGTTAGGGAACTTGGTTCCATTCTCTCTGTCACTTTCAGATACAACAATCA2050.101133185004662No Hit
GACGCTCGGGATTTATGGTCTGACCAACTGTCAGTATGTTAGCTAATAAAAA2040.10063985239488316No Hit
GTGGCGGGGGAGGATGACTGCGTCTCTTTGTCCAAGGCCAGCAGCTTTGCAG2040.10063985239488316No Hit
AATTCCGGGAATAACAATTTTCTGACCACCTTCTCTCTATGGAACACATAGA2030.1001465197851043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)