FastQCFastQC Report
Sat 31 Dec 2022
SRR5844255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202565
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT10020.4946560363340163No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC7790.38456791647125615No Hit
ATGTTAGGGTCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAA3540.17475871942339496No Hit
GCTTGAGGGAGAAATCAAGATGGACATTTCCCTATGTTAAAAAAAGTCAGTT3310.16340433934786366No Hit
TACAGTGGGAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGGC2660.1313158739170143No Hit
TAAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2610.12884753042233357No Hit
TACGATGGGAGTTCATTCAGTAAGCTTAGAGAGTACTCAGTCCAGATTGAAG2560.12637918692765285No Hit
ATAACCGGGCATCAGACAACAGGTAAGTTTTTTTTTAACTTATTGACCATGA2510.12391084343297214No Hit
GTTACAGGGCAGGATAAAAAACATTGTATTAAATTTCTGTCCAAGGAGAAGA2490.12292350603509984No Hit
TTAAATGGGCTGACGGAGGGAAACCAACAAAGATCAAAAGAGACAAAGAAGG2440.12045516254041912No Hit
TACCATGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG2280.11255646335744081No Hit
TAGCTGGGGATCAACCAGTCAGCATACATAACTTTCTTTTATGTGTGTTTCT2240.11058178856169625No Hit
GACGCTCGGGATTTATGGTCTGACCAACTGTCAGTATGTTAGCTAATAAAAA2230.11008811986276011No Hit
CAGGTGATCCGCCCGCCTCAGCCTCCCAAAGTGCTGGGATTGCAGGCGTGAG2230.11008811986276011No Hit
TTACCAGGGGGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAAA2200.10860711376595167No Hit
CAATTTGGGCATTTTTTGTCATGTAAGTTGAATTTATTCAATTTTTTCAACT2200.10860711376595167No Hit
CAGTTAGGGAACTTGGTTCCATTCTCTCTGTCACTTTCAGATACAACAATCA2190.10811344506701553No Hit
CTACTAAAATTGAAAAATTAGCTGGGCATGGTGGCAGGTGCCTGTAATCCCA2160.10663243897020709No Hit
AATAAAGGGTGCCACAGGTAGTTAGCTGGTCCCACTGGAGAATGGAATTAGA2120.10465776417446253No Hit
TGCTACGGGATAGGGGATCTGTAGCTTCATTCAAGATAACATTTGTTTGTTT2110.10416409547552637No Hit
CGCTGGGGGTACCTGCTTCAAATCACCCTGCACGTTTTCTCTTTGGAAAGAT2110.10416409547552637No Hit
GCCCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2110.10416409547552637No Hit
GGATTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2080.10268308937871795No Hit
TACAGGCGGGACACACACATATATATTTAACAAGTCTGTAGTTATTACCACA2070.1021894206797818No Hit
GCTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2060.10169575198084564No Hit
CCGACTGGGGGCATTTTAATGTATTTTAAAATAAATTTCAAAATTCTGCTAA2060.10169575198084564No Hit
TCAGTGGGGTCCTCAGTAACACCCACATAAACATACACACATGTGCAGAAAA2060.10169575198084564No Hit
GAATTAGGGAATCTTTCTTTGCTGTGACTGAAATGTATCCCTCGTTTATCAT2050.10120208328190951No Hit
TAAATGGGGCTTTGCCTTTTCTGTACACCTGTGAGACCAACAGATTGTCTAA2050.10120208328190951No Hit
TGGACCGGGGTAACCTACAAGCCTCCTAGTTCTGAAAAGTTGGAAGGGCATC2040.10070841458297336No Hit
TTATCTGGGGGGGCTCGGGGCAAGGTTCAGGGCTGTGAGACCCTGCGCCCTG2040.10070841458297336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)