FastQCFastQC Report
Sat 31 Dec 2022
SRR5844254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196798
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT9580.48679356497525383No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC7520.38211770444821597No Hit
GCTTGAGGGAGAAATCAAGATGGACATTTCCCTATGTTAAAAAAAGTCAGTT3280.1666683604508176No Hit
ATGTTAGGGTCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAA3110.15803006128111058No Hit
TACGATGGGAGTTCATTCAGTAAGCTTAGAGAGTACTCAGTCCAGATTGAAG2580.13109889328143579No Hit
TTACCAGGGGGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAAA2480.12601754082866695No Hit
TACAGGCGGGACACACACATATATATTTAACAAGTCTGTAGTTATTACCACA2340.11890364739479059No Hit
GACGCTCGGGATTTATGGTCTGACCAACTGTCAGTATGTTAGCTAATAAAAA2310.11737924165895995No Hit
GTTACAGGGCAGGATAAAAAACATTGTATTAAATTTCTGTCCAAGGAGAAGA2300.11687110641368306No Hit
TAAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2230.11331415969674488No Hit
TCAAACCGGGTCACTTATAAGAGTGCGTTTTTATCCATTCTTACTCTTGCTT2190.11128161871563735No Hit
AATAAAGGGTGCCACAGGTAGTTAGCTGGTCCCACTGGAGAATGGAATTAGA2140.10874094248925295No Hit
TAAGCGGGGAGCTTCCAAGATAAATATAAGACTAAAATTTGCTTGTTCCTTC2100.10670840150814541No Hit
ATAACCGGGCATCAGACAACAGGTAAGTTTTTTTTTAACTTATTGACCATGA2100.10670840150814541No Hit
GGATTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2050.10416772528176099No Hit
TCTAGGGTCCTTGGAGTATGACTATGAGACCATCCGCAACATCGACTGCTAC2020.10264331954593034No Hit
TACAGTGGGAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGGC2010.10213518430065346No Hit
TTAAATGGGCTGACGGAGGGAAACCAACAAAGATCAAAAGAGACAAAGAAGG2000.10162704905537656No Hit
ACATGGGGGGGTTGACTCCGGGGGCGCGGCGAGGAGAGACATGAGGCTGAGC1990.1011189138100997No Hit
TGCTTGGGGGACAAGACACAGAAACCTGAAGGCAGAACCCCAGGCCTCGGGG1980.10061077856482281No Hit
CATCGTGGGAGTATCATGAAACCATATGTAGTTATAAAAAGTCACTGGTAAA1980.10061077856482281No Hit
GTTGCTGGGGGGTAGCCAAGGCTGTAGTGAGCCTTGATAGCGCCACTGCATT1970.10010264331954592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)