FastQCFastQC Report
Sat 31 Dec 2022
SRR5844229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35555
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC4111.1559555618056532No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT3691.0378287160736888No Hit
TGTGCTGGGCGATCTTGCTTTGGGGCTAAACCTAGCCCCAAACCCACTCCAC2360.6637603712558009No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATT2310.6496976515258052No Hit
ATTATTGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC2240.630009843903811No Hit
AGACAGGGGATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTAT2200.6187596681198144No Hit
AGATTTGGGTCTGTGCGTGGGTCCTGGAGCTCAAGATCAGCTTGGGCAGCAG2190.6159471241738151No Hit
ATTGCTGGGGAATGGGCCATGGCAGACAACCAGTCCAAGAACCTGCCTAAAG2160.6075094923358177No Hit
CTAATAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG2140.6018844044438194No Hit
GAAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2130.5990718604978202No Hit
ATGAGTGGGAGCACCTGTAATCCAGTCTCCTTTCCAGAAAAGCTTCATTCTA2080.5850091407678245No Hit
GCTTTAGGGAAGATCACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCT2020.5681338770918296No Hit
GCATACGGGATGAACCATCTGCTTTTAATGATTTTCAGAGGCCAGCCATTTA1990.5596962452538321No Hit
AAGCAGGGGATAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAA1980.5568837013078329No Hit
AATACTGGGAGCCAGCCAGCTGCAGGTGGCATCTGCCACGAAGGAAGCGCCA1970.5540711573618338No Hit
AACAGTGGGGTCCCCATTGTCGCCTTTTCATAAAAGTCCTTAAGTTTTCCAT1970.5540711573618338No Hit
TTATATGGGGAATGCCTCAGAGCCAGGGAACAGGATGAACAAGAACAATCAG1960.5512586134158347No Hit
TAGTGTGGGGTAGTAACCAAATATTTTCACTCCAGATTTGTGTTTTCTCTGG1920.5400084376318379No Hit
GGGTAAGGGAGGACTGGTGCTAATAGAGGCAAGAGTGACTCCTGAGAGAAGG1880.5287582618478414No Hit
CTCTCGGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG1860.523133173955843No Hit
CTAGTGGTGGGAGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTT1820.5118829981718465No Hit
TATGTAGGGCATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAATAAT1780.5006328223878498No Hit
AGCTACGGGTCTCAAAACCCTCCTCATCATCTACTCCTTCGTCTTCTGGATC1780.5006328223878498No Hit
ACGCCTGGGCTACGCAGACTATAGAGAGAACTCGTACGTGCCGACCACTGGA1760.49500773449585145No Hit
GGACATGGGATCAGTCAGATGCCTGCCTAGCCAAAGCTGGACTAAGATTATT1750.49219519054985233No Hit
AACTGGGACGAGGTAATGGAGTTTTTCCCTTAGCTCCTGCTGTCACTACAGA1740.4893826466038532No Hit
ATGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTT1710.4809450147658557No Hit
CGCCTTGGGATGAGCAAAGATACCGTTTTAAATACAAATCAAGGAGAATATG1680.4725073829278582No Hit
AAAAAAGGGGATGTTGTGATGGGAGTTCAGTACAAGGATAAAGAGACTGGAG1660.46688229503585993No Hit
CGACGTGGGAATTATATTCTGGAAATTGCAGTATTAAGTGCCTTTTCCTCTC1650.4640697510898608No Hit
ATACATGGGACCCCTGAGCCAGTATACAGTACCATCCTCTCCCCCTACCAAG1630.4584446631978625No Hit
AAAGTAGGGAACGGGGGAGGCCGCAGTGGGACGTTCTGCGCCATCAGCATCG1620.4556321192518633No Hit
ATCTGAGGGAAAATATGCAGTAGCTAGGCTTACTGTGTTTAAGGAAATAAAA1590.44719448741386586No Hit
AGGTCGGGAACAAAGCATTTACCAACTACAGGATGTACTTCTTTCCAAAGAA1570.44156939952186747No Hit
TGGGGATGGGAGTGGAAATACAGAGATTTTGGTCAAAGGGTAAACATCTTCA1560.43875685557586835No Hit
GTAGTAGGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGA1560.43875685557586835No Hit
TCACTAGGGAACTTTATCAAAGTGACTGTACTGCAAATTAATTCATAATGCC1560.43875685557586835No Hit
CTGCCTGCCTTCCTGCCGTCCTTCCTTCTTCCCCGATCCATGCTACTTTTAG1540.4331317676838701No Hit
ACGCCAGGGAAACAGGAAGAAGCCAGTGACCGGAACAGCTCTAGGAATAACA1540.4331317676838701No Hit
GCACCAGGGATGCCAAGAAGCGAAATGTGCTCATTGGCCATTCCTACGGTGT1520.42750667979187174No Hit
TCCTTGGGGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCG1510.4246941358458726No Hit
TACTTAGGGCTAGAGCCGGGATTGGGCCAAAGGATCAGTTGAGAAACAGTTA1500.42188159189987345No Hit
CAGACAGGGGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCT1490.41906904795387423No Hit
GATACTGGGGTTTCACTACTTTATATTCATTTGAGTGTGATCCTAAAACACC1490.41906904795387423No Hit
TAAAGAGGGACTCTTTACCCTTTAACACTACACATGCACTTCATAAGAACAA1470.413443960061876No Hit
TACACGGGGTGTCTTGACATTACTTGGATGAACTGTGGCTAGCAAAATGGAA1470.413443960061876No Hit
AGCATGGGGTGCTTCTTAACTGGGACATGACATTTTTCTAGCTCTTATCAAG1450.4078188721698776No Hit
TTTTGTGGGACAACAAAACTTTAGAGTTCAAAAATCATCAGATTATCTTTAA1440.4050063282238785No Hit
CTTATTGGGTGTTGGGGGAAAGCAGAAGTCAAGCCAGCCGCGGGCCCCACAC1430.40219378427787933No Hit
ATACAAGGGAATGTTGTTGGAATGAGATGTGAATGGCAGATGGATAAAGGAA1430.40219378427787933No Hit
CTCAGCCCCTCAGAGAATGATCACCCTGAATTCATTGTCTCAGTGAAGGAAG1420.3993812403318802No Hit
ATCTGGGGAAATAAATAAAATAAAGCTAATGTTTAACTCTGGAGTGAAATAT1410.396568696385881No Hit
AGTTATGGGCTCCAGGCAGAGTGGGGCGGAAGGCTGCTGAGGATATGGGTCA1370.38531852060188443No Hit
AACAGCGGGAAGCTTATGTGGTACAGTAAGTGTTCTGTTCTGAGAAATACCT1360.38250597665588526No Hit
TTCATTGGGCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAG1340.3768808887638869No Hit
CATAAAGGGGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAGA1340.3768808887638869No Hit
ATAGGTGGGATAGATACAACATGACAAGAATACATAATGTAAGAGTATTTCA1340.3768808887638869No Hit
AAGGCTGGGGGTTTATGGTGATTGCATTGCTGGGCGTCTGGACATCTGTAGC1340.3768808887638869No Hit
TATACAGGGTTTTCAAACCCTGTATAAATAATCCATGCTGTTGGTCATAAGT1310.3684432569258895No Hit
AACCAGGGGACGAGGAGACCAACGAAGTGGAGATTCGATACGTGGACTACGG1300.3656307129798903No Hit
GATTAAGGGGCACAGCCAAGAGCGGTACCGGCATTGCGGCCATGTCTGTCAT1300.3656307129798903No Hit
ATTGAGGGGGGGGCCGGAAAGCAGGCAGAAGCCCGTCCTGGGTGGTGCTGGC1290.3628181690338912No Hit
TGTTGCGGGCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACT1290.3628181690338912No Hit
ACATAAGGGCTGAGGGTGCTTTGCGTATTTGGCCATTGCTCAGAAGAATAGA1280.360005625087892No Hit
AAATATGGGACCTTTTCTATGTTTAGATTTGTTTAAATACACTTATCCTCAT1270.3571930811418928No Hit
CGCTTTGGGACTAATCTGTAACATTTTACCAGTTCAGATGCCTGTAATGTGT1270.3571930811418928No Hit
ACTGAGGGGGGCCCGGGGTCGGGGCTGGGGAAGGGGAGCCCCAGGGTCGCCA1260.3543805371958937No Hit
TAGCTGGGAACTATTACATAGATATATAAGTGATCTCAGTTTCTTGTTTGCT1250.3515679932498945No Hit
GACAATGGATGGATCGGCGTGACCACCCCTTGCCGGAGGTGGCCCATGTCAA1250.3515679932498945No Hit
GCACAGGACTCAATATAGCAATTTATTCTTGATGTATGCAATTGCACATTGT1240.34875544930389535No Hit
CCTTTAGGGACGATCGGGAGCTACGCAAAATCTTAGCATACTCCTCAATTAC1240.34875544930389535No Hit
TAGCTTGGGGATGCTGCTAGTGTTGATAAGGTTTTGGAACTGAAAGAACACA1230.3459429053578962No Hit
GGAAAAGGGAATTACGGTCTTCTTTGAGGTCAAAATTCAGCCATCATGGTAG1210.3403178174658979No Hit
AAAACCGGGTCTCAGGTATGTCTTTATTAGCAGTATAAAAGCAGACTAATAC1170.3290676416819013No Hit
AAGCGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1160.3262550977359021No Hit
TACCTAGGGCCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG1140.32063000984390383No Hit
CCCCAATCACGAGATCTTCCTTCCGCTCTGAGGCAACCCCCTCGGAGCCTGT1140.32063000984390383No Hit
GGTCTGGGGGACGATTAAATTCTTCCAGCTTTCATTTTTCACTGAGATAATG1070.3009422022219097No Hit
TTCTATGGGAAGTGGAATTTACTGTTTTGTTAATAAAATATTTCTTAATACA1040.29250457038391225No Hit
CGGTCCGGGAATCTCAGACCTTGAAGGCTGATTCTCTAAAATAACTCATTCA1030.2896920264379131No Hit
TTAACGGGGTAGAGGTCCTCAGCCAGCAGAGCCACGTTCCTTATGAGCACCG1030.2896920264379131No Hit
GCTCTGGGACCCACTGAGGGGGCGGGCTGACAGAGCAGAGGAAGGAAGGGGG1010.2840669385459148No Hit
TAGCCTGGGCTGTCTTCATAATATAAGTGAAAATACTGTCATTTCAATTTTC1000.28125439459991564No Hit
TTTAGCGGGCCTCATCATAAAAATACAGTTGGTTTTTAGGGTGCCATATTGT1000.28125439459991564No Hit
TCCCCCAGAATTATCATTTTAGCACCTTCTCTCAGGGATGACCTATCAGTTT970.27281676276191813No Hit
GTGCCCGGCCCCTAAACTTGTATTTTTAAAACTTTTATTAAACCTTGAAAAA960.27000421881591896No Hit
TATAACGGGATTTCCTAAAATCACAGGACATTAAGGACCAATAGCATCTGTG960.27000421881591896No Hit
TGACCGGGGCATGCTGGGAGCTGGGGGGTGTGGGGGAAGGGGCTGCCGGCTT960.27000421881591896No Hit
CTGCTAAGTGGAGTTTATTATTTGAAGACTAAAATGGAATTCCATAGTTCCT960.27000421881591896No Hit
AGTACAGGGGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTAC950.26719167486991985No Hit
AGGGATGGGCATTGTAACCAAATTATTTTAAAATGTATAGTATAAAAAGTTA950.26719167486991985No Hit
GTAACTGGGGGTTGTCTTACCAGAGTGGGGATGTCCCTCTGATCTTTGCCCT940.2643791309239207No Hit
AAATCTGGGGCTAGGAGGTGGGGACCTGAGTTCTTGTCCTGGCTTTGGGACT930.2615665869779215No Hit
AGTGCAGGCGCCGCAACCCTGTTGGCCAACCACAGCCTGCGTGAGCTGGACC920.25875404303192234No Hit
GTAGATGGGACCCCCTCAGAAACCACTGTCATTAGTTTACAAAGTTAGCACT920.25875404303192234No Hit
TCCTCTGGGACCAGCATACAATGATGGAAGAATTAGATGTGGTGATATTCTT910.25594149908592323No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGAC910.25594149908592323No Hit
TTTAATGGGGAGAGCGCCGCGGCCCCGAGATGAAGCCGGCGGTGGACGAGAT900.25312895513992406No Hit
TTTTGAGGGGGTGGCTGGCAGCATCATTATCGGCATCTCCGTATCCAAGGCT900.25312895513992406No Hit
AACACTGGGCCCTGTAGTATGCTGGATATTTCTTGGTAAATGCATGAATAAA880.24750386724792572No Hit
ACACGAGGGTCTGTGTCTACTTTTTCACTTTTATCTTTTGTCATGAACTGCT880.24750386724792572No Hit
TTCGTGGGGGCAGTGGAAGCTCCTATGAGGGAGGAATATGCAGACTTCAAAC870.2446913233019266No Hit
TCTATGGGGGGGAAGTCACTCCGGTCAGAATTCAGGTACCCTCAATGGTGGA830.23344114751792996No Hit
GGTTACGGGATCCTAGAGTGAATGCACCAACTAACAGTATAGAATGCTGTCC830.23344114751792996No Hit
ACTTGGGGGTCTATGTCAATGTGTCTGTCCTTCACTCCTCCATTGTCTGCCG820.2306286035719308No Hit
TACAAAGGGAAGTAGACCAAATCTTGTTATTGAAGTGTTCTGAGCTGTATCT810.22781605962593166No Hit
TACCGGGACAGTTTATGCAAATGGAGCTTGGGGAAAGAGAGGTCGTGGCTGG810.22781605962593166No Hit
CACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTC790.22219097173393335No Hit
CTTGTGTCGGGTATTTGCCCATAGGCAACCAGAGCCACTTCCTCTGAACCCA770.21656588384193504No Hit
TACAGGGAAGGAAGAAGTCACAAAGCACAATAGCAAATACTTGAAACTGGAG770.21656588384193504No Hit
CGAACCGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT760.21375333989593587No Hit
CATTATGGGGTGGATCTGGAGCCTGGCACCATGGACTCTGTCAGGTCTGGAC760.21375333989593587No Hit
CATTAAGGGGCACGGGGGGCAGGACTCCGTCTACTGGAAGGGCTGGTTCGGG740.20812825200393756No Hit
CTTGTGCGGGGGGGACCGGCAAGATGGCGGCGCGGACAGCGTTCGGTGCTGT730.20531570805793842No Hit
AGCAGAGGTTGCGGTGAGCTGAGGTCGCGCCATTGCACTCCAGCCTGGGCAA720.20250316411193925No Hit
GTTTCCGGGCGATCTTTTATAGGGGATGAAATGTAATGAAATTGAAATGAAA720.20250316411193925No Hit
TCAACTGGAATCCAGTATTACTACACTATGATGTCATATATCTGGTAATTCA710.1996906201659401No Hit
ACCATGACTGTATGTAGTTTTAATAAAATCATTTAGAGTGAGTGAGTGCCAA710.1996906201659401No Hit
GTGTATGGGAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGTTTGGG710.1996906201659401No Hit
TATGAGGGGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT700.19687807621994094No Hit
AGTTGCGGGACCTGGGTCAGAGAGAGGAGTCCTTTTTGTCATGCCCTTAAGT670.18844044438194346No Hit
TCATATGGGAGCATTTCTCTGCCATTGCTTTCAATGAGGCCAGAGGCATCCT660.18562790043594432No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACA600.16875263675994936No Hit
TTCCAAAGGGGGAGTCAAGGGGCCCCATGCCTGCTTCTCTCCCAGCCCCAGC600.16875263675994936No Hit
GTATTGGGGATTATCATCCTCACCACAACCACTATCATCATCATCATCACCA560.15750246097595275No Hit
TTATCACTGGGAAGGGCAAAGCTTTGAGTGCCCAGAAGGGAAAGCTGTACCA540.15187737308395444No Hit
CAGGTAGGGGATCTGAGTTAGGGAGCCATGTGAATTCCACAAGAAGCACCAG510.143439741245957No Hit
CGATAAGGGCGACGCTCTTCCGATCTGATTATGGGACCCTTAATTCCATCCA510.143439741245957No Hit
AAATAGGGGTCAGAGGGAAAAAAATGTTGTGAAATAACAGTCCTTCCCAAGG490.13781465335395868No Hit
TAATATGGGTACGAATATGGGAATGCCCTGGAAGGGTCCACCTTTGTGAGCC460.12937702151596117No Hit
TCGGTGGGGAGCCAGCCAGTATGACCTATCTTAATCATCAGCTCAACTGTAA420.11812684573196455No Hit
TGCCAAGGGGACTACGCCAAGGTTGAGCTGGTTCCTCCCACCCCTGCTGAGA390.10968921389396709No Hit
GAACAGGGGGTTCTAATTTATTCCACATCATTCACTGAGATTCTCCACTCAC380.10687666994796793No Hit
ACCTAGGGTAGAGCTCAGTCAGGAAGGGGATGGGGCACCAAGCCAAGCCCCC380.10687666994796793No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACACTGTCTCTTA370.10406412600196878No Hit
GCTCTTGGGAGCAGCTGGAGAAAGAACTCAACACCTTACGAGTTACCCGTTC370.10406412600196878No Hit
ACTTGAGGGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACA360.10125158205596962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)