FastQCFastQC Report
Sat 31 Dec 2022
SRR5844228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35537
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC3911.1002616990742045No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT3751.0552382024368967No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATT2400.6753524495596139No Hit
ATGAGTGGGAGCACCTGTAATCCAGTCTCCTTTCCAGAAAAGCTTCATTCTA2380.6697245124799505No Hit
GAAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2360.664096575400287No Hit
ATTGCTGGGGAATGGGCCATGGCAGACAACCAGTCCAAGAACCTGCCTAAAG2350.6612826068604553No Hit
TGTGCTGGGCGATCTTGCTTTGGGGCTAAACCTAGCCCCAAACCCACTCCAC2350.6612826068604553No Hit
AGACAGGGGATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTAT2320.65284070124096No Hit
ACGCCTGGGCTACGCAGACTATAGAGAGAACTCGTACGTGCCGACCACTGGA2280.6415848270816332No Hit
GCATACGGGATGAACCATCTGCTTTTAATGATTTTCAGAGGCCAGCCATTTA2260.6359568900019698No Hit
GGGTAAGGGAGGACTGGTGCTAATAGAGGCAAGAGTGACTCCTGAGAGAAGG2200.6190730787629795No Hit
CTCTCGGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG2080.5853054562849987No Hit
AGATTTGGGTCTGTGCGTGGGTCCTGGAGCTCAAGATCAGCTTGGGCAGCAG2050.5768635506655035No Hit
CTAATAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1990.5599797394265132No Hit
CAGACAGGGGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCT1980.5571657708866815No Hit
TTATATGGGGAATGCCTCAGAGCCAGGGAACAGGATGAACAAGAACAATCAG1980.5571657708866815No Hit
CGCCTTGGGATGAGCAAAGATACCGTTTTAAATACAAATCAAGGAGAATATG1950.5487238652671863No Hit
AAGCAGGGGATAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAA1910.5374679911078594No Hit
GTAGTAGGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGA1880.5290260854883643No Hit
TAGTGTGGGGTAGTAACCAAATATTTTCACTCCAGATTTGTGTTTTCTCTGG1880.5290260854883643No Hit
AACAGTGGGGTCCCCATTGTCGCCTTTTCATAAAAGTCCTTAAGTTTTCCAT1860.5233981484087008No Hit
AACTGGGACGAGGTAATGGAGTTTTTCCCTTAGCTCCTGCTGTCACTACAGA1850.5205841798688691No Hit
GCTTTAGGGAAGATCACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCT1840.5177702113290373No Hit
ATTATTGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1820.5121422742493739No Hit
ACGCCAGGGAAACAGGAAGAAGCCAGTGACCGGAACAGCTCTAGGAATAACA1800.5065143371697105No Hit
CGACGTGGGAATTATATTCTGGAAATTGCAGTATTAAGTGCCTTTTCCTCTC1780.500886400090047No Hit
TACTTAGGGCTAGAGCCGGGATTGGGCCAAAGGATCAGTTGAGAAACAGTTA1760.49525846301038357No Hit
CTAGTGGTGGGAGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTT1750.4924444944705518No Hit
AATACTGGGAGCCAGCCAGCTGCAGGTGGCATCTGCCACGAAGGAAGCGCCA1740.4896305259307201No Hit
CTCAGCCCCTCAGAGAATGATCACCCTGAATTCATTGTCTCAGTGAAGGAAG1720.4840025888510567No Hit
AAAGTAGGGAACGGGGGAGGCCGCAGTGGGACGTTCTGCGCCATCAGCATCG1650.4643048090722346No Hit
ATCTGAGGGAAAATATGCAGTAGCTAGGCTTACTGTGTTTAAGGAAATAAAA1630.45867687199257107No Hit
TGGGGATGGGAGTGGAAATACAGAGATTTTGGTCAAAGGGTAAACATCTTCA1620.4558629034527394No Hit
ATACATGGGACCCCTGAGCCAGTATACAGTACCATCCTCTCCCCCTACCAAG1620.4558629034527394No Hit
TCCTTGGGGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCG1620.4558629034527394No Hit
AGCATGGGGTGCTTCTTAACTGGGACATGACATTTTTCTAGCTCTTATCAAG1620.4558629034527394No Hit
TTTTGTGGGACAACAAAACTTTAGAGTTCAAAAATCATCAGATTATCTTTAA1620.4558629034527394No Hit
TACCTAGGGCCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG1620.4558629034527394No Hit
AGTTATGGGCTCCAGGCAGAGTGGGGCGGAAGGCTGCTGAGGATATGGGTCA1590.4474209978332443No Hit
TATGTAGGGCATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAATAAT1580.44460702929341245No Hit
ACATAAGGGCTGAGGGTGCTTTGCGTATTTGGCCATTGCTCAGAAGAATAGA1560.4389790922137491No Hit
GGACATGGGATCAGTCAGATGCCTGCCTAGCCAAAGCTGGACTAAGATTATT1540.43335115513408556No Hit
AAAAAAGGGGATGTTGTGATGGGAGTTCAGTACAAGGATAAAGAGACTGGAG1540.43335115513408556No Hit
TCACTAGGGAACTTTATCAAAGTGACTGTACTGCAAATTAATTCATAATGCC1530.4305371865942539No Hit
ATACAAGGGAATGTTGTTGGAATGAGATGTGAATGGCAGATGGATAAAGGAA1520.42772321805442215No Hit
ATCTGGGGAAATAAATAAAATAAAGCTAATGTTTAACTCTGGAGTGAAATAT1500.4220952809747587No Hit
GCACCAGGGATGCCAAGAAGCGAAATGTGCTCATTGGCCATTCCTACGGTGT1490.41928131243492694No Hit
GACAATGGATGGATCGGCGTGACCACCCCTTGCCGGAGGTGGCCCATGTCAA1490.41928131243492694No Hit
AACAGCGGGAAGCTTATGTGGTACAGTAAGTGTTCTGTTCTGAGAAATACCT1490.41928131243492694No Hit
TGTTGCGGGCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACT1490.41928131243492694No Hit
ATTGAGGGGGGGGCCGGAAAGCAGGCAGAAGCCCGTCCTGGGTGGTGCTGGC1480.41646734389509527No Hit
TAAAGAGGGACTCTTTACCCTTTAACACTACACATGCACTTCATAAGAACAA1460.4108394068154318No Hit
GATTAAGGGGCACAGCCAAGAGCGGTACCGGCATTGCGGCCATGTCTGTCAT1460.4108394068154318No Hit
AGCTACGGGTCTCAAAACCCTCCTCATCATCTACTCCTTCGTCTTCTGGATC1430.40239750119593665No Hit
CGCTTTGGGACTAATCTGTAACATTTTACCAGTTCAGATGCCTGTAATGTGT1420.39958353265610486No Hit
AAGGCTGGGGGTTTATGGTGATTGCATTGCTGGGCGTCTGGACATCTGTAGC1410.3967695641162732No Hit
TATACAGGGTTTTCAAACCCTGTATAAATAATCCATGCTGTTGGTCATAAGT1400.39395559557644144No Hit
ATGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTT1400.39395559557644144No Hit
CATAAAGGGGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAGA1390.39114162703660976No Hit
CTGCCTGCCTTCCTGCCGTCCTTCCTTCTTCCCCGATCCATGCTACTTTTAG1360.38269972141711456No Hit
TACACGGGGTGTCTTGACATTACTTGGATGAACTGTGGCTAGCAAAATGGAA1360.38269972141711456No Hit
AAATATGGGACCTTTTCTATGTTTAGATTTGTTTAAATACACTTATCCTCAT1360.38269972141711456No Hit
CTTATTGGGTGTTGGGGGAAAGCAGAAGTCAAGCCAGCCGCGGGCCCCACAC1360.38269972141711456No Hit
CCTTTAGGGACGATCGGGAGCTACGCAAAATCTTAGCATACTCCTCAATTAC1360.38269972141711456No Hit
ACTGAGGGGGGCCCGGGGTCGGGGCTGGGGAAGGGGAGCCCCAGGGTCGCCA1320.3714438472577877No Hit
ATAGGTGGGATAGATACAACATGACAAGAATACATAATGTAAGAGTATTTCA1320.3714438472577877No Hit
GCACAGGACTCAATATAGCAATTTATTCTTGATGTATGCAATTGCACATTGT1300.3658159101781242No Hit
AAGCGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1260.3545600360187973No Hit
TAGCTGGGAACTATTACATAGATATATAAGTGATCTCAGTTTCTTGTTTGCT1210.3404901933196387No Hit
TAGCTTGGGGATGCTGCTAGTGTTGATAAGGTTTTGGAACTGAAAGAACACA1210.3404901933196387No Hit
AACCAGGGGACGAGGAGACCAACGAAGTGGAGATTCGATACGTGGACTACGG1200.33767622477980697No Hit
TTTAGCGGGCCTCATCATAAAAATACAGTTGGTTTTTAGGGTGCCATATTGT1170.3292343191603118No Hit
AGGTCGGGAACAAAGCATTTACCAACTACAGGATGTACTTCTTTCCAAAGAA1160.32642035062048No Hit
TCCCCCAGAATTATCATTTTAGCACCTTCTCTCAGGGATGACCTATCAGTTT1130.3179784450009849No Hit
TATAACGGGATTTCCTAAAATCACAGGACATTAAGGACCAATAGCATCTGTG1120.31516447646115314No Hit
GATACTGGGGTTTCACTACTTTATATTCATTTGAGTGTGATCCTAAAACACC1110.31235050792132146No Hit
GCTCTGGGACCCACTGAGGGGGCGGGCTGACAGAGCAGAGGAAGGAAGGGGG1110.31235050792132146No Hit
CCCCAATCACGAGATCTTCCTTCCGCTCTGAGGCAACCCCCTCGGAGCCTGT1100.30953653938148973No Hit
TTCATTGGGCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAG1090.306722570841658No Hit
AGGGATGGGCATTGTAACCAAATTATTTTAAAATGTATAGTATAAAAAGTTA1070.3010946337619945No Hit
AACACTGGGCCCTGTAGTATGCTGGATATTTCTTGGTAAATGCATGAATAAA1040.2926527281424994No Hit
AGTGCAGGCGCCGCAACCCTGTTGGCCAACCACAGCCTGCGTGAGCTGGACC1040.2926527281424994No Hit
GTAGATGGGACCCCCTCAGAAACCACTGTCATTAGTTTACAAAGTTAGCACT1040.2926527281424994No Hit
CTTGTGCGGGGGGGACCGGCAAGATGGCGGCGCGGACAGCGTTCGGTGCTGT1020.2870247910628359No Hit
TGACCGGGGCATGCTGGGAGCTGGGGGGTGTGGGGGAAGGGGCTGCCGGCTT970.2729549483636773No Hit
GGTCTGGGGGACGATTAAATTCTTCCAGCTTTCATTTTTCACTGAGATAATG950.2673270112840138No Hit
TCCTCTGGGACCAGCATACAATGATGGAAGAATTAGATGTGGTGATATTCTT940.26451304274418214No Hit
GGAAAAGGGAATTACGGTCTTCTTTGAGGTCAAAATTCAGCCATCATGGTAG940.26451304274418214No Hit
TACCGGGACAGTTTATGCAAATGGAGCTTGGGGAAAGAGAGGTCGTGGCTGG940.26451304274418214No Hit
TCTATGGGGGGGAAGTCACTCCGGTCAGAATTCAGGTACCCTCAATGGTGGA900.25325716858485525No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGAC890.2504432000450235No Hit
ACACGAGGGTCTGTGTCTACTTTTTCACTTTTATCTTTTGTCATGAACTGCT880.24762923150519178No Hit
GTTTCCGGGCGATCTTTTATAGGGGATGAAATGTAATGAAATTGAAATGAAA880.24762923150519178No Hit
CTGCTAAGTGGAGTTTATTATTTGAAGACTAAAATGGAATTCCATAGTTCCT870.24481526296536005No Hit
TTCTATGGGAAGTGGAATTTACTGTTTTGTTAATAAAATATTTCTTAATACA860.24200129442552834No Hit
GTGCCCGGCCCCTAAACTTGTATTTTTAAAACTTTTATTAAACCTTGAAAAA860.24200129442552834No Hit
CGGTCCGGGAATCTCAGACCTTGAAGGCTGATTCTCTAAAATAACTCATTCA860.24200129442552834No Hit
TTAACGGGGTAGAGGTCCTCAGCCAGCAGAGCCACGTTCCTTATGAGCACCG860.24200129442552834No Hit
AAATCTGGGGCTAGGAGGTGGGGACCTGAGTTCTTGTCCTGGCTTTGGGACT850.23918732588569658No Hit
TAGCCTGGGCTGTCTTCATAATATAAGTGAAAATACTGTCATTTCAATTTTC830.23355938880603314No Hit
TTCGTGGGGGCAGTGGAAGCTCCTATGAGGGAGGAATATGCAGACTTCAAAC830.23355938880603314No Hit
TTATCACTGGGAAGGGCAAAGCTTTGAGTGCCCAGAAGGGAAAGCTGTACCA830.23355938880603314No Hit
CATTAAGGGGCACGGGGGGCAGGACTCCGTCTACTGGAAGGGCTGGTTCGGG830.23355938880603314No Hit
TCAACTGGAATCCAGTATTACTACACTATGATGTCATATATCTGGTAATTCA820.23074542026620143No Hit
TACAAAGGGAAGTAGACCAAATCTTGTTATTGAAGTGTTCTGAGCTGTATCT820.23074542026620143No Hit
TTTTGAGGGGGTGGCTGGCAGCATCATTATCGGCATCTCCGTATCCAAGGCT810.2279314517263697No Hit
AGCAGAGGTTGCGGTGAGCTGAGGTCGCGCCATTGCACTCCAGCCTGGGCAA790.22230351464670622No Hit
AGTACAGGGGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTAC780.21948954610687454No Hit
TACAGGGAAGGAAGAAGTCACAAAGCACAATAGCAAATACTTGAAACTGGAG760.21386160902721107No Hit
GTAACTGGGGGTTGTCTTACCAGAGTGGGGATGTCCCTCTGATCTTTGCCCT760.21386160902721107No Hit
TTTAATGGGGAGAGCGCCGCGGCCCCGAGATGAAGCCGGCGGTGGACGAGAT750.21104764048737934No Hit
ACTTGGGGGTCTATGTCAATGTGTCTGTCCTTCACTCCTCCATTGTCTGCCG750.21104764048737934No Hit
AAAACCGGGTCTCAGGTATGTCTTTATTAGCAGTATAAAAGCAGACTAATAC740.20823367194754763No Hit
CTTGTGTCGGGTATTTGCCCATAGGCAACCAGAGCCACTTCCTCTGAACCCA720.2026057348678842No Hit
GGTTACGGGATCCTAGAGTGAATGCACCAACTAACAGTATAGAATGCTGTCC690.194163829248389No Hit
AGTTGCGGGACCTGGGTCAGAGAGAGGAGTCCTTTTTGTCATGCCCTTAAGT670.18853589216872554No Hit
TCATATGGGAGCATTTCTCTGCCATTGCTTTCAATGAGGCCAGAGGCATCCT660.18572192362889384No Hit
GTGTATGGGAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGTTTGGG650.1829079550890621No Hit
CATTATGGGGTGGATCTGGAGCCTGGCACCATGGACTCTGTCAGGTCTGGAC610.1716520809297352No Hit
GTATTGGGGATTATCATCCTCACCACAACCACTATCATCATCATCATCACCA610.1716520809297352No Hit
TTCCAAAGGGGGAGTCAAGGGGCCCCATGCCTGCTTCTCTCCCAGCCCCAGC600.16883811238990348No Hit
CACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTC590.16602414385007175No Hit
ACCATGACTGTATGTAGTTTTAATAAAATCATTTAGAGTGAGTGAGTGCCAA580.16321017531024No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACA550.15476826969074486No Hit
CGAACCGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT520.1463263640712497No Hit
TATGAGGGGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT480.1350704899119228No Hit
TAACAGGCGCTCCCCTGGACAAGGCACAGGGCTTCTTAAAGTACGTCTCTTG470.13225652137209107No Hit
CTTTGTGGGCATCCATATGCATTTGTGCAACTTCAAACATATTGGGTGCTTC450.12662858429242763No Hit
TCGGTGGGGAGCCAGCCAGTATGACCTATCTTAATCATCAGCTCAACTGTAA430.12100064721276417No Hit
TGCCAAGGGGACTACGCCAAGGTTGAGCTGGTTCCTCCCACCCCTGCTGAGA430.12100064721276417No Hit
TAATATGGGTACGAATATGGGAATGCCCTGGAAGGGTCCACCTTTGTGAGCC410.11537271013310071No Hit
AAATAGGGGTCAGAGGGAAAAAAATGTTGTGAAATAACAGTCCTTCCCAAGG400.112558741593269No Hit
ACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTAC390.10974477305343727No Hit
AACAACGGGAAGAGAGCGTGTGCCGAAGGCCTCGGCCTTTCACATTCGGGAA390.10974477305343727No Hit
ACGTACGGGGAGCGACTGCGGCGGCGGCGGCGGCGGCGGCGGCGCCTCGGAG390.10974477305343727No Hit
GCTCTTGGGAGCAGCTGGAGAAAGAACTCAACACCTTACGAGTTACCCGTTC370.10411683597377382No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACACTGTCTCTTATACAC360.1013028674339421No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)