FastQCFastQC Report
Sat 31 Dec 2022
SRR5844227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34842
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC4031.156650020090695No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT3390.9729636645427931No Hit
TGTGCTGGGCGATCTTGCTTTGGGGCTAAACCTAGCCCCAAACCCACTCCAC2270.6515125423339647No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATT2270.6515125423339647No Hit
GAAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2230.6400321451122208No Hit
AGACAGGGGATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTAT2220.6371620458067849No Hit
GCATACGGGATGAACCATCTGCTTTTAATGATTTTCAGAGGCCAGCCATTTA2180.625681648585041No Hit
ATTGCTGGGGAATGGGCCATGGCAGACAACCAGTCCAAGAACCTGCCTAAAG2170.622811549279605No Hit
AACAGTGGGGTCCCCATTGTCGCCTTTTCATAAAAGTCCTTAAGTTTTCCAT2170.622811549279605No Hit
AGATTTGGGTCTGTGCGTGGGTCCTGGAGCTCAAGATCAGCTTGGGCAGCAG2150.6170713506687331No Hit
CTCTCGGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG2030.5826301590035016No Hit
TTATATGGGGAATGCCTCAGAGCCAGGGAACAGGATGAACAAGAACAATCAG1950.5596693645600137No Hit
GCTTTAGGGAAGATCACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCT1910.5481889673382698No Hit
AAGCAGGGGATAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGGTATAA1890.542448768727398No Hit
TCCTTGGGGTTCGCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCG1880.539578669421962No Hit
AATACTGGGAGCCAGCCAGCTGCAGGTGGCATCTGCCACGAAGGAAGCGCCA1860.53383847081109No Hit
TACTTAGGGCTAGAGCCGGGATTGGGCCAAAGGATCAGTTGAGAAACAGTTA1850.5309683715056541No Hit
TAGTGTGGGGTAGTAACCAAATATTTTCACTCCAGATTTGTGTTTTCTCTGG1840.5280982722002181No Hit
GGGTAAGGGAGGACTGGTGCTAATAGAGGCAAGAGTGACTCCTGAGAGAAGG1830.5252281728947822No Hit
ATGAGTGGGAGCACCTGTAATCCAGTCTCCTTTCCAGAAAAGCTTCATTCTA1800.5166178749784742No Hit
CGCCTTGGGATGAGCAAAGATACCGTTTTAAATACAAATCAAGGAGAATATG1780.5108776763676023No Hit
TATGTAGGGCATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAATAAT1780.5108776763676023No Hit
CAGACAGGGGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCT1730.4965271798404225No Hit
ACGCCAGGGAAACAGGAAGAAGCCAGTGACCGGAACAGCTCTAGGAATAACA1730.4965271798404225No Hit
ACGCCTGGGCTACGCAGACTATAGAGAGAACTCGTACGTGCCGACCACTGGA1720.4936570805349865No Hit
AAAGTAGGGAACGGGGGAGGCCGCAGTGGGACGTTCTGCGCCATCAGCATCG1700.4879168819241146No Hit
CTAATAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1700.4879168819241146No Hit
ATTATTGGGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACC1690.4850467826186786No Hit
AGGTCGGGAACAAAGCATTTACCAACTACAGGATGTACTTCTTTCCAAAGAA1680.48217668331324265No Hit
CGACGTGGGAATTATATTCTGGAAATTGCAGTATTAAGTGCCTTTTCCTCTC1650.47356638539693474No Hit
ATACATGGGACCCCTGAGCCAGTATACAGTACCATCCTCTCCCCCTACCAAG1640.47069628609149883No Hit
ATCTGAGGGAAAATATGCAGTAGCTAGGCTTACTGTGTTTAAGGAAATAAAA1640.47069628609149883No Hit
TACCTAGGGCCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG1640.47069628609149883No Hit
CTGCCTGCCTTCCTGCCGTCCTTCCTTCTTCCCCGATCCATGCTACTTTTAG1630.46782618678606286No Hit
AGCATGGGGTGCTTCTTAACTGGGACATGACATTTTTCTAGCTCTTATCAAG1620.4649560874806269No Hit
CCTTTAGGGACGATCGGGAGCTACGCAAAATCTTAGCATACTCCTCAATTAC1610.4620859881751908No Hit
TTTTGTGGGACAACAAAACTTTAGAGTTCAAAAATCATCAGATTATCTTTAA1590.45634578956431887No Hit
AGCTACGGGTCTCAAAACCCTCCTCATCATCTACTCCTTCGTCTTCTGGATC1580.4534756902588829No Hit
ATCTGGGGAAATAAATAAAATAAAGCTAATGTTTAACTCTGGAGTGAAATAT1570.45060559095344693No Hit
AACTGGGACGAGGTAATGGAGTTTTTCCCTTAGCTCCTGCTGTCACTACAGA1560.44773549164801096No Hit
CTTATTGGGTGTTGGGGGAAAGCAGAAGTCAAGCCAGCCGCGGGCCCCACAC1550.44486539234257505No Hit
AAAAAAGGGGATGTTGTGATGGGAGTTCAGTACAAGGATAAAGAGACTGGAG1550.44486539234257505No Hit
ATGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTT1530.4391251937317031No Hit
CTAGTGGTGGGAGAGAGTGGGGACGTCCGGCTTCGGAGCGGGAGTGTTCGTT1510.4333849951208312No Hit
GACAATGGATGGATCGGCGTGACCACCCCTTGCCGGAGGTGGCCCATGTCAA1510.4333849951208312No Hit
TCACTAGGGAACTTTATCAAAGTGACTGTACTGCAAATTAATTCATAATGCC1490.42764479650995924No Hit
ACATAAGGGCTGAGGGTGCTTTGCGTATTTGGCCATTGCTCAGAAGAATAGA1480.42477469720452327No Hit
CATAAAGGGGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAGA1470.4219045978990873No Hit
GTAGTAGGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGA1470.4219045978990873No Hit
GGACATGGGATCAGTCAGATGCCTGCCTAGCCAAAGCTGGACTAAGATTATT1470.4219045978990873No Hit
CTCAGCCCCTCAGAGAATGATCACCCTGAATTCATTGTCTCAGTGAAGGAAG1470.4219045978990873No Hit
AGTTATGGGCTCCAGGCAGAGTGGGGCGGAAGGCTGCTGAGGATATGGGTCA1460.41903449859365133No Hit
AACAGCGGGAAGCTTATGTGGTACAGTAAGTGTTCTGTTCTGAGAAATACCT1460.41903449859365133No Hit
TGGGGATGGGAGTGGAAATACAGAGATTTTGGTCAAAGGGTAAACATCTTCA1450.41616439928821536No Hit
GCACCAGGGATGCCAAGAAGCGAAATGTGCTCATTGGCCATTCCTACGGTGT1410.4046840020664715No Hit
GCACAGGACTCAATATAGCAATTTATTCTTGATGTATGCAATTGCACATTGT1400.40181390276103557No Hit
TATACAGGGTTTTCAAACCCTGTATAAATAATCCATGCTGTTGGTCATAAGT1390.3989438034555996No Hit
CGCTTTGGGACTAATCTGTAACATTTTACCAGTTCAGATGCCTGTAATGTGT1380.39607370415016363No Hit
TAAAGAGGGACTCTTTACCCTTTAACACTACACATGCACTTCATAAGAACAA1370.39320360484472766No Hit
AACCAGGGGACGAGGAGACCAACGAAGTGGAGATTCGATACGTGGACTACGG1370.39320360484472766No Hit
ATAGGTGGGATAGATACAACATGACAAGAATACATAATGTAAGAGTATTTCA1350.3874634062338557No Hit
ACACGAGGGTCTGTGTCTACTTTTTCACTTTTATCTTTTGTCATGAACTGCT1350.3874634062338557No Hit
AAGGCTGGGGGTTTATGGTGATTGCATTGCTGGGCGTCTGGACATCTGTAGC1320.37885310831754776No Hit
TTTAGCGGGCCTCATCATAAAAATACAGTTGGTTTTTAGGGTGCCATATTGT1310.3759830090121118No Hit
TACACGGGGTGTCTTGACATTACTTGGATGAACTGTGGCTAGCAAAATGGAA1300.3731129097066758No Hit
ATTGAGGGGGGGGCCGGAAAGCAGGCAGAAGCCCGTCCTGGGTGGTGCTGGC1300.3731129097066758No Hit
GATACTGGGGTTTCACTACTTTATATTCATTTGAGTGTGATCCTAAAACACC1290.37024281040123985No Hit
ATACAAGGGAATGTTGTTGGAATGAGATGTGAATGGCAGATGGATAAAGGAA1270.36450261179036797No Hit
ACTGAGGGGGGCCCGGGGTCGGGGCTGGGGAAGGGGAGCCCCAGGGTCGCCA1260.361632512484932No Hit
AAATATGGGACCTTTTCTATGTTTAGATTTGTTTAAATACACTTATCCTCAT1260.361632512484932No Hit
TTCATTGGGCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAG1240.35589231387406006No Hit
TGTTGCGGGCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACT1240.35589231387406006No Hit
TAGCTGGGAACTATTACATAGATATATAAGTGATCTCAGTTTCTTGTTTGCT1190.3415418173468802No Hit
GTAGATGGGACCCCCTCAGAAACCACTGTCATTAGTTTACAAAGTTAGCACT1180.33867171804144425No Hit
AGTGCAGGCGCCGCAACCCTGTTGGCCAACCACAGCCTGCGTGAGCTGGACC1170.3358016187360083No Hit
GGTCTGGGGGACGATTAAATTCTTCCAGCTTTCATTTTTCACTGAGATAATG1150.3300614201251363No Hit
AGGGATGGGCATTGTAACCAAATTATTTTAAAATGTATAGTATAAAAAGTTA1110.31858102290339246No Hit
TGACCGGGGCATGCTGGGAGCTGGGGGGTGTGGGGGAAGGGGCTGCCGGCTT1110.31858102290339246No Hit
GATTAAGGGGCACAGCCAAGAGCGGTACCGGCATTGCGGCCATGTCTGTCAT1090.3128408242925205No Hit
CTTGTGCGGGGGGGACCGGCAAGATGGCGGCGCGGACAGCGTTCGGTGCTGT1090.3128408242925205No Hit
TCCTCTGGGACCAGCATACAATGATGGAAGAATTAGATGTGGTGATATTCTT1080.30997072498708456No Hit
TAGCTTGGGGATGCTGCTAGTGTTGATAAGGTTTTGGAACTGAAAGAACACA1080.30997072498708456No Hit
GGAAAAGGGAATTACGGTCTTCTTTGAGGTCAAAATTCAGCCATCATGGTAG1080.30997072498708456No Hit
TTTAATGGGGAGAGCGCCGCGGCCCCGAGATGAAGCCGGCGGTGGACGAGAT1070.3071006256816486No Hit
AAAACCGGGTCTCAGGTATGTCTTTATTAGCAGTATAAAAGCAGACTAATAC1060.3042305263762126No Hit
GTGCCCGGCCCCTAAACTTGTATTTTTAAAACTTTTATTAAACCTTGAAAAA1060.3042305263762126No Hit
AAATCTGGGGCTAGGAGGTGGGGACCTGAGTTCTTGTCCTGGCTTTGGGACT1060.3042305263762126No Hit
CCCCAATCACGAGATCTTCCTTCCGCTCTGAGGCAACCCCCTCGGAGCCTGT1030.2956202284599047No Hit
TCCCCCAGAATTATCATTTTAGCACCTTCTCTCAGGGATGACCTATCAGTTT1010.28988002984903277No Hit
TTAACGGGGTAGAGGTCCTCAGCCAGCAGAGCCACGTTCCTTATGAGCACCG990.2841398312381609No Hit
AACACTGGGCCCTGTAGTATGCTGGATATTTCTTGGTAAATGCATGAATAAA980.28126973193272486No Hit
AAGCGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC970.2783996326272889No Hit
TTATCACTGGGAAGGGCAAAGCTTTGAGTGCCCAGAAGGGAAAGCTGTACCA960.2755295333218529No Hit
TATAACGGGATTTCCTAAAATCACAGGACATTAAGGACCAATAGCATCTGTG960.2755295333218529No Hit
CGGTCCGGGAATCTCAGACCTTGAAGGCTGATTCTCTAAAATAACTCATTCA940.269789334710981No Hit
TTTTGAGGGGGTGGCTGGCAGCATCATTATCGGCATCTCCGTATCCAAGGCT910.2611790367946731No Hit
TTCTATGGGAAGTGGAATTTACTGTTTTGTTAATAAAATATTTCTTAATACA910.2611790367946731No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGAC900.2583089374892371No Hit
GTAACTGGGGGTTGTCTTACCAGAGTGGGGATGTCCCTCTGATCTTTGCCCT890.25543883818380114No Hit
AGTACAGGGGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTAC880.2525687388783652No Hit
TCAACTGGAATCCAGTATTACTACACTATGATGTCATATATCTGGTAATTCA870.24969863957292923No Hit
TACCGGGACAGTTTATGCAAATGGAGCTTGGGGAAAGAGAGGTCGTGGCTGG870.24969863957292923No Hit
TAGCCTGGGCTGTCTTCATAATATAAGTGAAAATACTGTCATTTCAATTTTC860.24682854026749326No Hit
GGTTACGGGATCCTAGAGTGAATGCACCAACTAACAGTATAGAATGCTGTCC860.24682854026749326No Hit
TACAAAGGGAAGTAGACCAAATCTTGTTATTGAAGTGTTCTGAGCTGTATCT800.22960794443487742No Hit
AGTTGCGGGACCTGGGTCAGAGAGAGGAGTCCTTTTTGTCATGCCCTTAAGT790.22673784512944145No Hit
GCTCTGGGACCCACTGAGGGGGCGGGCTGACAGAGCAGAGGAAGGAAGGGGG780.22386774582400548No Hit
CTTGTGTCGGGTATTTGCCCATAGGCAACCAGAGCCACTTCCTCTGAACCCA750.2152574479076976No Hit
CATTATGGGGTGGATCTGGAGCCTGGCACCATGGACTCTGTCAGGTCTGGAC750.2152574479076976No Hit
TCTATGGGGGGGAAGTCACTCCGGTCAGAATTCAGGTACCCTCAATGGTGGA750.2152574479076976No Hit
GTTTCCGGGCGATCTTTTATAGGGGATGAAATGTAATGAAATTGAAATGAAA750.2152574479076976No Hit
TTCCAAAGGGGGAGTCAAGGGGCCCCATGCCTGCTTCTCTCCCAGCCCCAGC750.2152574479076976No Hit
TTCGTGGGGGCAGTGGAAGCTCCTATGAGGGAGGAATATGCAGACTTCAAAC740.21238734860226163No Hit
AGCAGAGGTTGCGGTGAGCTGAGGTCGCGCCATTGCACTCCAGCCTGGGCAA710.20377705068595373No Hit
GTGTATGGGAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGTTTGGG700.20090695138051778No Hit
TACAGGGAAGGAAGAAGTCACAAAGCACAATAGCAAATACTTGAAACTGGAG690.19803685207508182No Hit
CACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACCATTC680.19516675276964585No Hit
CTGCTAAGTGGAGTTTATTATTTGAAGACTAAAATGGAATTCCATAGTTCCT670.19229665346420985No Hit
TCATATGGGAGCATTTCTCTGCCATTGCTTTCAATGAGGCCAGAGGCATCCT630.180816256242466No Hit
GTATTGGGGATTATCATCCTCACCACAACCACTATCATCATCATCATCACCA580.16646575971528613No Hit
CATTAAGGGGCACGGGGGGCAGGACTCCGTCTACTGGAAGGGCTGGTTCGGG570.16359566040985019No Hit
ACTTGGGGGTCTATGTCAATGTGTCTGTCCTTCACTCCTCCATTGTCTGCCG530.1521152631881063No Hit
AAATAGGGGTCAGAGGGAAAAAAATGTTGTGAAATAACAGTCCTTCCCAAGG520.14924516388267034No Hit
ACCATGACTGTATGTAGTTTTAATAAAATCATTTAGAGTGAGTGAGTGCCAA510.14637506457723437No Hit
CGAACCGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT500.1435049652717984No Hit
ACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTAC480.13776476666092646No Hit
TCGGTGGGGAGCCAGCCAGTATGACCTATCTTAATCATCAGCTCAACTGTAA480.13776476666092646No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACA480.13776476666092646No Hit
CAGGTAGGGGATCTGAGTTAGGGAGCCATGTGAATTCCACAAGAAGCACCAG460.13202456805005452No Hit
GAACAGGGGGTTCTAATTTATTCCACATCATTCACTGAGATTCTCCACTCAC440.1262843694391826No Hit
ACCTAGGGTAGAGCTCAGTCAGGAAGGGGATGGGGCACCAAGCCAAGCCCCC440.1262843694391826No Hit
TATGAGGGGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT430.12341427013374663No Hit
CTTTGTGGGCATCCATATGCATTTGTGCAACTTCAAACATATTGGGTGCTTC430.12341427013374663No Hit
CGATAAGGGCGACGCTCTTCCGATCTGATTATGGGACCCTTAATTCCATCCA420.12054417082831066No Hit
AACAACGGGAAGAGAGCGTGTGCCGAAGGCCTCGGCCTTTCACATTCGGGAA400.11480397221743871No Hit
TGCCAAGGGGACTACGCCAAGGTTGAGCTGGTTCCTCCCACCCCTGCTGAGA390.11193387291200274No Hit
ACTTGAGGGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACA380.10906377360656679No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACACTGTCTCTTATACAC370.10619367430113082No Hit
GCTCTTGGGAGCAGCTGGAGAAAGAACTCAACACCTTACGAGTTACCCGTTC350.10045347569025889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)