FastQCFastQC Report
Sat 31 Dec 2022
SRR5844217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287300
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC6950.2419074138531152No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT5820.2025757048381483No Hit
TCTTCCGGGGAATGCTTTAAACCACATTTATACTATTTTTATTCTGTTAACA4530.15767490428123912No Hit
GAATAGGGGGAGCTGCAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTG4380.15245388096066828No Hit
TCAGAGGGACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGAT4180.14549251653324052No Hit
TAGCAAGGGGTATCCTGACTAAGGCCAAGGATGATTCAGAATTAGAAGGACA4130.14375217542638355No Hit
GTTCGGGGATGAACTATGAGGGTTCTTTATCCTAGTTTCTATTTATGAAGAT4070.14166376609815523No Hit
TTTTCAGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGC3990.1388792203271841No Hit
TAGTCAGGGAAGCTAAATAAGTGCTTTTAATTTTATTACCACAAAATTGCAG3960.13783501566306997No Hit
TGCTTCGGGCTACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGC3910.13609467455621302No Hit
CGATAGGGTGCTCATTCAGCATGATGTTGACTGTGGGTTTGTCATTGATGAC3860.13435433344935607No Hit
AAGTTGGGGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAGGAAGCAGC3850.13400626522798467No Hit
TCACCTGGGAGCACTCAGGTTCTCCTGTGACAGCTGGATTTACCCCCAAAAT3840.1336581970066133No Hit
CAGAATGGGCTGTCCTCTTCTGTGAGTCAGTCAGCGAGTGCTTGGGATCCGC3820.1329620605638705No Hit
TGCTATGGGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAA3810.13261399234249913No Hit
CCTGCAGCTAGTCCTAGTGAACCTCACCCCGAGGCCCTGCATGGGCCAGCCC3810.13261399234249913No Hit
AAAACAGGGAGCCAACCAATTCCTCAGTAATTCTGCAGTGAACCAAAAGAGT3790.13191785589975635No Hit
GTGCGAGGTAGGCTGAAGTGATTCTCCTGCCTCAGCCTCGCAAGTAGCTGGG3770.13122171945701358No Hit
ACTCGCGGGAATCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTA3770.13122171945701358No Hit
AAGCATGGGTATTTATAGGGTACATGAGATGTTTTGATACAGGCATACAATG3750.1305255830142708No Hit
GTGGTTGGGGCCACAAGCCTGAAAAGACAGTTCTCCTGTGGCAGTGGCGATG3740.1301775147928994No Hit
GTTACTGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTGC3730.129829446571528No Hit
CAATGTGGGAGTAGCAGATCTGGTAAGTGAGCAGGGGCAGCCTGGGGTGCTG3700.12878524190741386No Hit
TATGCCGGGGAGTAGAAAACTAAATGCTGTTCATATCACTGGGAAAGCTCAT3680.12808910546467106No Hit
GAAGTCGGGTGGGCCTTCTTTATTAATGGTATATGTCTATATCAATGAGTTA3660.12739296902192832No Hit
TGCTCAGGATTCTGATGGATTCAGCAAATTATTATCATCATGACTTTTTATG3650.12704490080055691No Hit
CACACTGGGGTGACAGTGTCTCTTCTCAGGCCTGCAGTGTGTACTATTTGCT3640.12669683257918551No Hit
TGACATGGGTACTTAAACACCTATCAGCTATTTTTATCTTTAACCTTTTCTG3630.12634876435781414No Hit
ATTCTAGGGATGCCAAAACCACACAGTGTTTCACTTAATGATACCGAAACAA3630.12634876435781414No Hit
GCATAGGGACGGGCGAGAAGCCGCACCGCTGCGCTGTGTGTGGCCGTCGCTT3630.12634876435781414No Hit
AGCAATGGGAGGTGTGAGCCACCACGCCCGCCCTAAAAGTGGATTCTTTTTT3630.12634876435781414No Hit
TCAATTGGGCAGCCATCTTGGAGGAAATTGGCCATTTCTGCTTAGATGGTTG3620.12600069613644274No Hit
CTCACGAAGGGAACCATTTTTCAGTGGTCTGGATACGAAGTTTACAGTTTCA3610.12565262791507137No Hit
TATCTGGGGGAGTGGTTAAAGTTTTCAAAGCGGTTGGCAGCAGCGGCGCTTG3600.12530455969369997No Hit
TTTTATGGGGAGGCTTAGACTTTACCATATAGACAAGGAGAAATATTGAAAG3580.12460842325095718No Hit
ATCTACGGGTGCTGACTGCAATGTGATAGAGATAGATGATACCCTCGACGAC3580.12460842325095718No Hit
ACTACCGGGATGTACACACTTGTTTAATTAGGAGAAGCTATTCCAACCTGAT3570.12426035502958581No Hit
GAATTAGGGCGCTCAGCTCTGTGCTGTGTGAAAGCACCACCAGCCAGTGTGT3550.12356421858684301No Hit
GTCCTTGGGTCTATTCATTTAGTACATTTTCTTAACCTTGAAAACAGACATT3550.12356421858684301No Hit
TTGCACGGGCAGACGCTGGTATGGGGGCTCGGACCCCTCACTGGGGACAGGG3550.12356421858684301No Hit
CACAAAGGGATCTGCTTAGATGTGAGAGTTGATGCCATAATAATAAAAGTTA3540.12321615036547164No Hit
CATCCAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC3510.12217194570135746No Hit
AGCTTTGGGGAAGAGTGCAGATACACTTTGGGGGATCCAAAAGGAGCTGCAA3510.12217194570135746No Hit
AGGCATGGGGGCACGTAGCAGTCTTGAAGTTCCTACTTCCTACCACTCTAAT3510.12217194570135746No Hit
GCACAAGGGAAGTACTGGATATTTAAGTGTGAGTAATGTCTTCATTAGAAAA3490.12147580925861469No Hit
TATGAGGGGACCCTTGGAAGATAAAGCTGGGTCTTCAGGAACTCAGTGTCTG3470.12077967281587192No Hit
ACATATGGGAACATGGAGGCATGTGGTATCATGATATTCTTCACTAAATTTA3460.12043160459450052No Hit
TAACCTTGGGCATCTAAAGGTGACAGCTTGTGGGCAGAACCATGCACAGGAA3440.11973546815175774No Hit
GACTCTGGGTTAGTAAAGGAATACTTTCAAGTCATCCCAAATATGGTTCCAT3420.11903933170901496No Hit
CTCTCAGGGACATTAACCTCTTTATGATTTTACAGTTTCTTTGTTTGTGATA3420.11903933170901496No Hit
TTTAATGGGACAGACAAGATCTTCAGGCAGATGGACACCAACAATGACGGCA3400.1183431952662722No Hit
AGATTAGGGGGGGCCTACTCACTTATTCTTTCACCCCCTCTCCTCCTGCTCA3390.11799512704490081No Hit
AGTCTAGGGGGAAGGAATGTGCTTGCCTGTCAGGTGCAGGGAGAATTCAGTG3370.11729899060215802No Hit
AAAATTGGGACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTT3370.11729899060215802No Hit
AGTTTAGGGCAGATGAGAAGATTTTTGTCTTTTGTAGCACTGATAACCAGGA3360.11695092238078664No Hit
TTCTCTGGTATAGCAAACTGGATTAATAAATAGAAACACATAGTGTAGTCTT3360.11695092238078664No Hit
GCCAGTGGGGGGGCTAGTGCAAGGGACTATGAATCCCTTGGAGAGGAAATCA3350.11660285415941525No Hit
ATTGTTGGGGCGGGACAGGGTGGCTGGCACGAGATGTGTGGGACTGGGACGC3350.11660285415941525No Hit
ACAAGGGGTAATACACAGAATCCTGCAACTTTTTGCACAGCTGGTATCTGTC3340.11625478593804386No Hit
CGACTTGGGGCGTTTCCAGGAGGGAGCGGCCTTTGCTCAGCGCGAGACGGCT3340.11625478593804386No Hit
CTGAAAGGGATATCTTACCAATGTATTGTTTTGAAGAGGGTTATTTTGGGGG3330.11590671771667246No Hit
GTAACTGGGATGTCTGCTGCTATCACAAAATACCTGCGACTGGAGAATATAT3330.11590671771667246No Hit
CTCGTGGGGTTTTTGATGTAGGTGTTTATTACTAAATACTTCCCTCTTAAAA3330.11590671771667246No Hit
TTCATCGGGGACTGGACAGTGGGGTTTCTACCATGAATGAAATGAAGAGTTA3330.11590671771667246No Hit
TCAAGTGGGCCATACAGCCCCAGGCAATGGGCACAGCCTTCCTCCCCAGAGG3320.11555864949530108No Hit
CAGGTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC3320.11555864949530108No Hit
GCGCAAGGGAGCCCCTTCACATCTAAAAAAACCATACTGCTTTTAACTGATT3320.11555864949530108No Hit
AAAGCTGGGAGATTTGTTGCCGTCATGTCGGCTGCGATTGCAGCTCTGGCCG3320.11555864949530108No Hit
GCCTTTGGGTAGAGGGGCTACTGGATGTGGGAAAACCACACAGGTTCCCCAG3300.1148625130525583No Hit
TTCAATGGGAAGGGAAGCCATCGGTACCTTCTCAGGTACTTTGTTTCTGGAT3300.1148625130525583No Hit
TTATTTGGGAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGCAAAC3290.1145144448311869No Hit
TTACCTGGGGACATATACACTTAATATAGTTCTAATAAAAATCCTTCCAAAA3280.11416637660981552No Hit
CGATTTGGGTTCCAGGGTTGGTCTTCCTGAGAAGGGGATGGATGAGGTAACA3280.11416637660981552No Hit
TTGTTAGGGCTGTTGCACAGTTGCCCTTGGAGTTGCTGCATCATCTTGATTT3270.11381830838844413No Hit
TACTTAGGGGGTTTGTCCAATATGGCGGCGCCCAGTGGCGGTGTGAACTGTG3230.11242603550295857No Hit
AAATGCAGAGAAAAAGATGTTACCTTGAAAAATGAAGTAAGAAATCCTAATA3210.11172989906021581No Hit
ACGCCGGGACTGCAATCCGGAAAGAAATAATATCACTGCAGAAAGAATGTGA3210.11172989906021581No Hit
ATCACGGGAGCATGACTTATTACCATTGATAATAACCTTGATTACCTGTCTG3210.11172989906021581No Hit
ACTTTTGGGAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA3190.11103376261747303No Hit
TCTGGCGGGGGGATGAATCCTTCTAATGGTGATTCTCATTTAGAGTAAAAGA3180.11068569439610164No Hit
ATGTAAGGGAATGTTGCTATTTAGCAATGTGCTTGGTTTTAAAGAAATTCTC3170.11033762617473025No Hit
TCATTAGGGAAAGAAAAACCTAAAGAGGATGCATATGAATATAAAAAGCGTT3160.10998955795335885No Hit
GCTCTAGGGCGGATCAGATGGCTATATCTCACTATTTTGCTTTCTGCCTTCA3160.10998955795335885No Hit
CTTCTTGGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTG3150.10964148973198747No Hit
ATCCTGGGGCATTGAAGAATAACCAAGATGTCATCAGGGCCACTCAGCCCTT3150.10964148973198747No Hit
TCTAGTCGGGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCAT3150.10964148973198747No Hit
CAGCATGGGACACAGAGATCTCATTCCTTGTGAAAGAATCCTCGTTTTGGAA3150.10964148973198747No Hit
CTTATGGGGCTGCAGAATGGGCTGTTGGAAGGTATAATAGCTTATCGAACCA3140.10929342151061608No Hit
ATGTGCGGGCGATCTTAGTCGGGGAACATCCTTATTTTACTTTTCAGTACTG3140.10929342151061608No Hit
CGCGGGTTACTCAGATGTACACAATTGCTCTTACAATTACAAAGCCGATGCT3130.10894535328924469No Hit
GGGTTTGGGCGATCTGGTTTCGGGACAGGACGAAAAGGAGGTTGTGCTAAAG3120.1085972850678733No Hit
GCCTGAGGGCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTG3120.1085972850678733No Hit
AACCATGGGATGCTGAAAGATTGAGTACCGTCCCCTTAGGATCAAGAAATAA3110.1082492168465019No Hit
CAAAGCGGGGGGCGCGGGGGATTACATTGGATCTGCAGATTCATGCAATCCC3100.10790114862513052No Hit
ATTCCAGGGGTATTCTGGCGCCGCTGACTCCCAGGGCTGGGCTATCCTCTGA3100.10790114862513052No Hit
TATGTAGGGGACTATGTGGACAGAGGCTATTTCAGTATAGAGGTAAAAATTA3090.10755308040375913No Hit
ATTTCAGGGTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCCGGGTGGTG3090.10755308040375913No Hit
ACGTTTGGGTCCAGCCACTAGATTGTCCCTCTCTGGGAAGCCCTAAACCAAC3080.10720501218238776No Hit
CTAAGAGGGGGTTATTTGAAACCAATCCATGGCAACGCTGGTGCTTGATACA3070.10685694396101635No Hit
TGTCCAGGGACAGAGGCTTAGAAACTGGCGCAAAGTCATTCGTTCATTCAAC3070.10685694396101635No Hit
GATTGGCAAGCTTACCAGTCTTCAGCTGCAACACCTGTTTATGATCCTGGCC3070.10685694396101635No Hit
ACAAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3070.10685694396101635No Hit
AAAGCTGGGCGATCTCTAGTTGGGAAATCAAAGGAACTTGTTTCTTCAAGCT3060.10650887573964496No Hit
CTTATGGGGATACTAACTTAGGAGTTTCAAATAACAGAAAATCTGTCTGAAT3050.10616080751827359No Hit
GGTAATGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT3050.10616080751827359No Hit
GTGACTGGGAAAAACAGTTTTGTTTTGTTTTATTTTCTGAACGAAGATAATG3040.1058127392969022No Hit
CACCTTGGGGACAAAATTTTAAGTGTTTGTGCACTTACCCCATCTGTTGGCC3040.1058127392969022No Hit
TCTGCCAGGGGGGGGAGTTGATTTGCACTGCTCCGGGTGTCGGCTCCGGCGG3040.1058127392969022No Hit
CTTCAGGGAGCCAGAGAGCTGTCATTTCAGTACCGGGATTCAGAATTGATCC3030.10546467107553081No Hit
GTAGATGGGCATCCATTTGGGCTGAGGGTAGACTGGCTTCACCCTTAGACTT3000.10442046641141664No Hit
ACTAACGGGATGATTAAAGCATCAAGGAAGCAAATATAAAGTTTAATGAAAA2980.10372432996867385No Hit
TGCTAAGGGTCTCACATAGGGAGCTTTCTCCATGTATACTATTTAGAGGTTT2980.10372432996867385No Hit
TGCATCTGGGTGTTGCTTTGACAATTATTCTATTTTTCTCTGTTTTATTCAT2970.10337626174730247No Hit
GTTTCAGGGCTGCAGACACCCTTGCTCCTGAGTCATTATTTTCTTTCATGTT2960.10302819352593108No Hit
AAAACAGGGTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTTCATTGTGGACA2940.10233205708318832No Hit
GTCTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2940.10233205708318832No Hit
GCTTTGGGATTCAACTGATTTACAACACACATGATGACTGAGGCATTCGGGA2930.10198398886181691No Hit
CCATAGGGGAACATAGTATTTGTAATAAGCAAAACTTAAAAGTATGCAACTC2930.10198398886181691No Hit
TGTTGTGGGAGGAGAATTTACAATAATTAGTGAAAATTATTCAATTCTCATA2930.10198398886181691No Hit
GTATTGGGAGATTAGAGTAAGTAGGACTAGAATAATTTTAGTAAATATTCTT2920.10163592064044552No Hit
GAAACTGGGGATTTTATGAAAACTAAAAACTTCAAAGATGTTATCAAGAAAG2920.10163592064044552No Hit
GCTTACGGGAATATCACAGCATAACCCCACCCTTTACATTTTGTGCAGTGAT2910.10128785241907415No Hit
CCTATAGGGCAGAGAAAGCTAAGCAGGTGGCATTTACGCTGAATCTTGAATT2910.10128785241907415No Hit
AATTGCGGGTAGCTAGTTCCTAATGAATATTTTGTTGGAAGCTCTATCCTCT2900.10093978419770276No Hit
GTCATTGGGTACATTTCAAAGGTCTGACTAAATCAGTCTTTGGGTGGCTTGA2900.10093978419770276No Hit
GACCTCGGGCTGTGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTA2890.10059171597633135No Hit
ACTGCGGGACTTCACCTACAGAGACACACATAGACTGAAAATGAAGAGATGA2890.10059171597633135No Hit
ATCCGGGACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATGG2880.10024364775495997No Hit
TCACGAGGTTATGGAAACTCTGAGAAGCTGAATGATACATGCAGGGGATATT2880.10024364775495997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)