FastQCFastQC Report
Sat 31 Dec 2022
SRR5844216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286381
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC6560.22906547571242505No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT5050.1763385140774004No Hit
GAATAGGGGGAGCTGCAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTG4660.16272029219815562No Hit
TCAGAGGGACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGAT4300.1501496258480835No Hit
TCTTCCGGGGAATGCTTTAAACCACATTTATACTATTTTTATTCTGTTAACA4230.14770532961334723No Hit
TCAATTGGGCAGCCATCTTGGAGGAAATTGGCCATTTCTGCTTAGATGGTTG4180.1459594037313928No Hit
AAGCATGGGTATTTATAGGGTACATGAGATGTTTTGATACAGGCATACAATG4150.1449118482022201No Hit
GTTCGGGGATGAACTATGAGGGTTCTTTATCCTAGTTTCTATTTATGAAGAT4110.14351510749665655No Hit
TAGCAAGGGGTATCCTGACTAAGGCCAAGGATGATTCAGAATTAGAAGGACA4010.14002325573274765No Hit
GTATACGGGTACTGAATAGGAAACTGGGAGTGAGGCCCCAGCAATCTGAATA4000.13967407055635675No Hit
CACAAAGGGATCTGCTTAGATGTGAGAGTTGATGCCATAATAATAAAAGTTA3990.13932488537996585No Hit
TTGCACGGGCAGACGCTGGTATGGGGGCTCGGACCCCTCACTGGGGACAGGG3970.13862651502718407No Hit
CAATGTGGGAGTAGCAGATCTGGTAAGTGAGCAGGGGCAGCCTGGGGTGCTG3930.13722977432162048No Hit
CGATAGGGTGCTCATTCAGCATGATGTTGACTGTGGGTTTGTCATTGATGAC3810.1330395522049298No Hit
TTTTCAGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGC3800.1326903670285389No Hit
TGCTTCGGGCTACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGC3800.1326903670285389No Hit
TGCTCAGGATTCTGATGGATTCAGCAAATTATTATCATCATGACTTTTTATG3750.13094444114658443No Hit
TTTAATGGGACAGACAAGATCTTCAGGCAGATGGACACCAACAATGACGGCA3740.13059525597019356No Hit
ATTCTAGGGATGCCAAAACCACACAGTGTTTCACTTAATGATACCGAAACAA3740.13059525597019356No Hit
TGACATGGGTACTTAAACACCTATCAGCTATTTTTATCTTTAACCTTTTCTG3710.12954770044102087No Hit
TCACCTGGGAGCACTCAGGTTCTCCTGTGACAGCTGGATTTACCCCCAAAAT3680.1285001449118482No Hit
TGCTATGGGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAA3670.12815095973545731No Hit
ACATATGGGAACATGGAGGCATGTGGTATCATGATATTCTTCACTAAATTTA3660.1278017745590664No Hit
GTGCGAGGTAGGCTGAAGTGATTCTCCTGCCTCAGCCTCGCAAGTAGCTGGG3660.1278017745590664No Hit
ATTGTTGGGGCGGGACAGGGTGGCTGGCACGAGATGTGTGGGACTGGGACGC3660.1278017745590664No Hit
GTCCTTGGGTCTATTCATTTAGTACATTTTCTTAACCTTGAAAACAGACATT3650.12745258938267554No Hit
CACACTGGGGTGACAGTGTCTCTTCTCAGGCCTGCAGTGTGTACTATTTGCT3630.12675421902989373No Hit
TTGTTAGGGCTGTTGCACAGTTGCCCTTGGAGTTGCTGCATCATCTTGATTT3630.12675421902989373No Hit
CTCGTGGGGTTTTTGATGTAGGTGTTTATTACTAAATACTTCCCTCTTAAAA3630.12675421902989373No Hit
ACTCGCGGGAATCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTA3630.12675421902989373No Hit
AAAACAGGGAGCCAACCAATTCCTCAGTAATTCTGCAGTGAACCAAAAGAGT3620.12640503385350285No Hit
GTAACTGGGATGTCTGCTGCTATCACAAAATACCTGCGACTGGAGAATATAT3620.12640503385350285No Hit
CTCACGAAGGGAACCATTTTTCAGTGGTCTGGATACGAAGTTTACAGTTTCA3620.12640503385350285No Hit
GTGGTTGGGGCCACAAGCCTGAAAAGACAGTTCTCCTGTGGCAGTGGCGATG3590.12535747832433017No Hit
CGATTTGGGTTCCAGGGTTGGTCTTCCTGAGAAGGGGATGGATGAGGTAACA3570.1246591079715484No Hit
AAAGCTGGGAGATTTGTTGCCGTCATGTCGGCTGCGATTGCAGCTCTGGCCG3560.1243099227951575No Hit
TAGTCAGGGAAGCTAAATAAGTGCTTTTAATTTTATTACCACAAAATTGCAG3560.1243099227951575No Hit
CATCCAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC3550.12396073761876661No Hit
GCCTGAGGGCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTG3530.12326236726598482No Hit
GAAGTCGGGTGGGCCTTCTTTATTAATGGTATATGTCTATATCAATGAGTTA3530.12326236726598482No Hit
CGCGGGTTACTCAGATGTACACAATTGCTCTTACAATTACAAAGCCGATGCT3510.12256399691320305No Hit
AGCAATGGGAGGTGTGAGCCACCACGCCCGCCCTAAAAGTGGATTCTTTTTT3510.12256399691320305No Hit
TTATTTGGGAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGCAAAC3490.12186562656042124No Hit
AGATTAGGGGGGGCCTACTCACTTATTCTTTCACCCCCTCTCCTCCTGCTCA3480.12151644138403038No Hit
AGCTTTGGGGAAGAGTGCAGATACACTTTGGGGGATCCAAAAGGAGCTGCAA3480.12151644138403038No Hit
TAACCTTGGGCATCTAAAGGTGACAGCTTGTGGGCAGAACCATGCACAGGAA3460.12081807103124859No Hit
GACTCTGGGTTAGTAAAGGAATACTTTCAAGTCATCCCAAATATGGTTCCAT3430.11977051550207592No Hit
GCATAGGGACGGGCGAGAAGCCGCACCGCTGCGCTGTGTGTGGCCGTCGCTT3430.11977051550207592No Hit
CCTGCAGCTAGTCCTAGTGAACCTCACCCCGAGGCCCTGCATGGGCCAGCCC3420.11942133032568501No Hit
GCCTTTGGGTAGAGGGGCTACTGGATGTGGGAAAACCACACAGGTTCCCCAG3390.11837377479651234No Hit
AAGTTGGGGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAGGAAGCAGC3390.11837377479651234No Hit
ATTTCAGGGTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCCGGGTGGTG3380.11802458962012145No Hit
GCGCAAGGGAGCCCCTTCACATCTAAAAAAACCATACTGCTTTTAACTGATT3370.11767540444373055No Hit
TATGTAGGGGACTATGTGGACAGAGGCTATTTCAGTATAGAGGTAAAAATTA3370.11767540444373055No Hit
CGTATTGGGGACGCTCTTCCGATCTCTCGTAGGGCGTGTTACTGAAGGTAAT3370.11767540444373055No Hit
AACCATGGGATGCTGAAAGATTGAGTACCGTCCCCTTAGGATCAAGAAATAA3360.11732621926733966No Hit
ATCACGGGAGCATGACTTATTACCATTGATAATAACCTTGATTACCTGTCTG3350.11697703409094878No Hit
GCACAAGGGAAGTACTGGATATTTAAGTGTGAGTAATGTCTTCATTAGAAAA3350.11697703409094878No Hit
GAATTAGGGCGCTCAGCTCTGTGCTGTGTGAAAGCACCACCAGCCAGTGTGT3340.11662784891455788No Hit
TATGAGGGGACCCTTGGAAGATAAAGCTGGGTCTTCAGGAACTCAGTGTCTG3340.11662784891455788No Hit
ACAAGGGGTAATACACAGAATCCTGCAACTTTTTGCACAGCTGGTATCTGTC3340.11662784891455788No Hit
ATTCCAGGGGTATTCTGGCGCCGCTGACTCCCAGGGCTGGGCTATCCTCTGA3330.11627866373816699No Hit
TTCAATGGGAAGGGAAGCCATCGGTACCTTCTCAGGTACTTTGTTTCTGGAT3320.11592947856177609No Hit
TTACCTGGGGACATATACACTTAATATAGTTCTAATAAAAATCCTTCCAAAA3310.1155802933853852No Hit
AAAGCTGGGCGATCTCTAGTTGGGAAATCAAAGGAACTTGTTTCTTCAAGCT3300.11523110820899432No Hit
ACGCCGGGACTGCAATCCGGAAAGAAATAATATCACTGCAGAAAGAATGTGA3300.11523110820899432No Hit
GGTAATGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT3300.11523110820899432No Hit
CTTATGGGGCTGCAGAATGGGCTGTTGGAAGGTATAATAGCTTATCGAACCA3290.11488192303260342No Hit
AAGGTAGGGACTTCTGCAGTGCGACATCCAGAAATGAGCTGATGTCAATGCT3290.11488192303260342No Hit
ACAAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3280.11453273785621253No Hit
GGGTTTGGGCGATCTGGTTTCGGGACAGGACGAAAAGGAGGTTGTGCTAAAG3260.11383436750343075No Hit
CAGGTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC3250.11348518232703986No Hit
GTTACTGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTGC3250.11348518232703986No Hit
GTGTTGGGGTGCTCCGGCTTGATTCCTAATCAGTTACAACACCCAAGTCTTC3240.11313599715064895No Hit
TTCTCTGGTATAGCAAACTGGATTAATAAATAGAAACACATAGTGTAGTCTT3240.11313599715064895No Hit
AAAATTGGGACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTT3240.11313599715064895No Hit
GACCTCGGGCTGTGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTA3220.11243762679786719No Hit
TCAAGTGGGCCATACAGCCCCAGGCAATGGGCACAGCCTTCCTCCCCAGAGG3210.11208844162147628No Hit
CTTCTTGGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTG3210.11208844162147628No Hit
ACTACCGGGATGTACACACTTGTTTAATTAGGAGAAGCTATTCCAACCTGAT3210.11208844162147628No Hit
TTCATCGGGGACTGGACAGTGGGGTTTCTACCATGAATGAAATGAAGAGTTA3210.11208844162147628No Hit
TCATTAGGGAAAGAAAAACCTAAAGAGGATGCATATGAATATAAAAAGCGTT3200.1117392564450854No Hit
TGTTGTGGGAGGAGAATTTACAATAATTAGTGAAAATTATTCAATTCTCATA3200.1117392564450854No Hit
CTCTCAGGGACATTAACCTCTTTATGATTTTACAGTTTCTTTGTTTGTGATA3180.1110408860923036No Hit
TGCATCTGGGTGTTGCTTTGACAATTATTCTATTTTTCTCTGTTTTATTCAT3180.1110408860923036No Hit
CAGAATGGGCTGTCCTCTTCTGTGAGTCAGTCAGCGAGTGCTTGGGATCCGC3180.1110408860923036No Hit
TACTTAGGGGGTTTGTCCAATATGGCGGCGCCCAGTGGCGGTGTGAACTGTG3170.11069170091591272No Hit
ATGTAAGGGAATGTTGCTATTTAGCAATGTGCTTGGTTTTAAAGAAATTCTC3170.11069170091591272No Hit
CTGAAAGGGATATCTTACCAATGTATTGTTTTGAAGAGGGTTATTTTGGGGG3160.11034251573952183No Hit
CTTCAGGGAGCCAGAGAGCTGTCATTTCAGTACCGGGATTCAGAATTGATCC3160.11034251573952183No Hit
CAAAGCGGGGGGCGCGGGGGATTACATTGGATCTGCAGATTCATGCAATCCC3160.11034251573952183No Hit
AGGCATGGGGGCACGTAGCAGTCTTGAAGTTCCTACTTCCTACCACTCTAAT3160.11034251573952183No Hit
GCTCTAGGGCGGATCAGATGGCTATATCTCACTATTTTGCTTTCTGCCTTCA3150.10999333056313093No Hit
AAAACAGGGTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTTCATTGTGGACA3140.10964414538674003No Hit
AGTCTAGGGGGAAGGAATGTGCTTGCCTGTCAGGTGCAGGGAGAATTCAGTG3140.10964414538674003No Hit
GAAACTGGGGATTTTATGAAAACTAAAAACTTCAAAGATGTTATCAAGAAAG3140.10964414538674003No Hit
GTATTGGGAGATTAGAGTAAGTAGGACTAGAATAATTTTAGTAAATATTCTT3130.10929496021034917No Hit
ATCTACGGGTGCTGACTGCAATGTGATAGAGATAGATGATACCCTCGACGAC3130.10929496021034917No Hit
TTGTGTGGGGGTTGCACACCAGAGGACGCACTCAGGGGAGAGGCCGTATGTG3120.10894577503395826No Hit
CAGCATGGGACACAGAGATCTCATTCCTTGTGAAAGAATCCTCGTTTTGGAA3110.10859658985756737No Hit
CACAAAGGTAAGATAATTTTATTGTCTATTTAAAGACAGGCCTTGGCAATTG3100.10824740468117647No Hit
CGGCACGGGATCTTTTGGCGGGAAAAGATAAAGACAATCCTGGTACTTCTTC3090.10789821950478559No Hit
TCAAACGGGCGATGTGGAGCTTCTAAAACTGGAATGACAGGAATGGCCTCCT3090.10789821950478559No Hit
GTGGTCGGGGGTTTTTCATTGATGATAAACGACTACAGACTTAGTAGTGGAG3090.10789821950478559No Hit
CGACTTGGGGCGTTTCCAGGAGGGAGCGGCCTTTGCTCAGCGCGAGACGGCT3090.10789821950478559No Hit
TTAGTTGGGCATCTTGAAGGTGTACACAAGGTCACAAAGCATTGACCAAGAA3080.1075490343283947No Hit
TAGCCAGGGCAGCGCCAGCGGCTGGAGCGGGAGCGCATGGAGCGGGAACGGC3070.1071998491520038No Hit
TGCTAAGGGTCTCACATAGGGAGCTTTCTCCATGTATACTATTTAGAGGTTT3070.1071998491520038No Hit
TTCAACGGGGGACCTAGGCTATCTTACCATGAAGGTTGTTTTGCTTATTGTA3060.10685066397561291No Hit
TATGCTGGATTGCTGACTTTTATATTATGGGAGGGAAAAAATAACATTAATA3040.10615229362283113No Hit
TTTTATGGGGAGGCTTAGACTTTACCATATAGACAAGGAGAAATATTGAAAG3040.10615229362283113No Hit
ACGATAGGGCAACTCATCATCGGTGACTGGTTATCACACCATCGCTGGCCCC3030.10580310844644024No Hit
ACTGCGGGACTTCACCTACAGAGACACACATAGACTGAAAATGAAGAGATGA3030.10580310844644024No Hit
ATGTGCGGGCGATCTTAGTCGGGGAACATCCTTATTTTACTTTTCAGTACTG3030.10580310844644024No Hit
TGCCTAGGGGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTT3030.10580310844644024No Hit
ATAAATGGGGGAAACAATCTTTATGCTGAACAAAGAAGGCACAATTAAATAA3020.10545392327004934No Hit
TTCTCAGGGGAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGTC3000.10475555291726757No Hit
TACGGGGGGCCCTGGCAGATACCTTTTTTGCATGGTCACTCAGTGCTCCTGG3000.10475555291726757No Hit
TAACAGGGGGGGCTGAGAAGGCTCAGGACACCGAGCTGGCCGCCTACCTGGA3000.10475555291726757No Hit
TCTGGCGGGGGGATGAATCCTTCTAATGGTGATTCTCATTTAGAGTAAAAGA2990.10440636774087667No Hit
TCACGAGGTTATGGAAACTCTGAGAAGCTGAATGATACATGCAGGGGATATT2980.10405718256448578No Hit
CTAAGAGGGGGTTATTTGAAACCAATCCATGGCAACGCTGGTGCTTGATACA2970.10370799738809487No Hit
TCTGCCAGGGGGGGGAGTTGATTTGCACTGCTCCGGGTGTCGGCTCCGGCGG2970.10370799738809487No Hit
TGTCCAGGGACAGAGGCTTAGAAACTGGCGCAAAGTCATTCGTTCATTCAAC2970.10370799738809487No Hit
GACAGAGTGAGATCCTGTCTCAAAAAAAATAAAACAGATGTAATGCTTACCT2970.10370799738809487No Hit
GGCTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2960.10335881221170398No Hit
GTGACTGGGAAAAACAGTTTTGTTTTGTTTTATTTTCTGAACGAAGATAATG2940.1026604418589222No Hit
AAATGCAGAGAAAAAGATGTTACCTTGAAAAATGAAGTAAGAAATCCTAATA2940.1026604418589222No Hit
TTGCGCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT2930.10231125668253131No Hit
CTTGTTCGGGCAATAGGTGGAAAATAAAGGGATAGGAAAAATCTACCAAGCA2930.10231125668253131No Hit
GCCGCTGGGAGTATATATGTGGTAAGGAAATGCCGATTCTTCATTATAAACA2920.10196207150614041No Hit
ATGTTTGGGTCTGGAGACAGAGTTAGCAGGATACAAAATTGCCATTGTATGG2910.10161288632974953No Hit
TGCAACGGGTCAGTCTGGAAGATCCCCAGACACATGCTAACAGGATCTACAG2910.10161288632974953No Hit
GCTTACGGGAATATCACAGCATAACCCCACCCTTTACATTTTGTGCAGTGAT2910.10161288632974953No Hit
ACTTTTGGGAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA2900.10126370115335864No Hit
ACTAACGGGATGATTAAAGCATCAAGGAAGCAAATATAAAGTTTAATGAAAA2890.10091451597696774No Hit
ATCCGGGACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATGG2890.10091451597696774No Hit
AGTTTAGGGCAGATGAGAAGATTTTTGTCTTTTGTAGCACTGATAACCAGGA2890.10091451597696774No Hit
CATTCTGGGTGATCCTTCATGTTGCCCACCCACTTCCCTCTGTTCACCTATT2890.10091451597696774No Hit
ATTGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2880.10056533080057685No Hit
ACGAATGGGCGTCATACCATGGACCATCTATTCTGTAACCTGCTTAATATTT2880.10056533080057685No Hit
AAAAGGGGTTAGAACTTCAGTAACAGATTGGTTTGACACTAATGATTAGGCA2880.10056533080057685No Hit
AATTGCGGGTAGCTAGTTCCTAATGAATATTTTGTTGGAAGCTCTATCCTCT2870.10021614562418597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)