FastQCFastQC Report
Sat 31 Dec 2022
SRR5844215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281978
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC6310.2237763229755513No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT5220.18512082502890295No Hit
GAATAGGGGGAGCTGCAGAGCTACGCAGCCTTCGGTGCAGTCGTCACTCGTG4570.1620693812992503No Hit
TCAGAGGGACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCACGAT4140.14681996467809547No Hit
TGCTATGGGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAA4050.14362822631552816No Hit
GTTCGGGGATGAACTATGAGGGTTCTTTATCCTAGTTTCTATTTATGAAGAT3940.13972721276127925No Hit
GTGGTTGGGGCCACAAGCCTGAAAAGACAGTTCTCCTGTGGCAGTGGCGATG3930.13937257516543844No Hit
TGCTTCGGGCTACCGTGCCTGGCCTAAACCTTACGCTTTTGAGGTTGAGTGC3920.13901793756959763No Hit
CAATGTGGGAGTAGCAGATCTGGTAAGTGAGCAGGGGCAGCCTGGGGTGCTG3920.13901793756959763No Hit
TCACCTGGGAGCACTCAGGTTCTCCTGTGACAGCTGGATTTACCCCCAAAAT3910.13866329997375682No Hit
TCTTCCGGGGAATGCTTTAAACCACATTTATACTATTTTTATTCTGTTAACA3870.13724474959039357No Hit
TCAATTGGGCAGCCATCTTGGAGGAAATTGGCCATTTCTGCTTAGATGGTTG3870.13724474959039357No Hit
GCATAGGGACGGGCGAGAAGCCGCACCGCTGCGCTGTGTGTGGCCGTCGCTT3810.13511692401534872No Hit
CTCACGAAGGGAACCATTTTTCAGTGGTCTGGATACGAAGTTTACAGTTTCA3810.13511692401534872No Hit
CACAAAGGGATCTGCTTAGATGTGAGAGTTGATGCCATAATAATAAAAGTTA3800.1347622864195079No Hit
CCTGCAGCTAGTCCTAGTGAACCTCACCCCGAGGCCCTGCATGGGCCAGCCC3770.13369837363198547No Hit
CGATAGGGTGCTCATTCAGCATGATGTTGACTGTGGGTTTGTCATTGATGAC3730.13227982324862222No Hit
TGACATGGGTACTTAAACACCTATCAGCTATTTTTATCTTTAACCTTTTCTG3710.13157054805694063No Hit
TAGTCAGGGAAGCTAAATAAGTGCTTTTAATTTTATTACCACAAAATTGCAG3680.13050663526941816No Hit
CTCTCAGGGACATTAACCTCTTTATGATTTTACAGTTTCTTTGTTTGTGATA3660.12979736007773657No Hit
TTTTCAGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGC3660.12979736007773657No Hit
GTATACGGGTACTGAATAGGAAACTGGGAGTGAGGCCCCAGCAATCTGAATA3620.12837880969437332No Hit
CAGAATGGGCTGTCCTCTTCTGTGAGTCAGTCAGCGAGTGCTTGGGATCCGC3610.1280241720985325No Hit
AGTTTAGGGCAGATGAGAAGATTTTTGTCTTTTGTAGCACTGATAACCAGGA3600.1276695345026917No Hit
CATCCAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC3580.12696025931101007No Hit
TATGTAGGGGACTATGTGGACAGAGGCTATTTCAGTATAGAGGTAAAAATTA3560.12625098411932845No Hit
CGATTTGGGTTCCAGGGTTGGTCTTCCTGAGAAGGGGATGGATGAGGTAACA3560.12625098411932845No Hit
AGCTTTGGGGAAGAGTGCAGATACACTTTGGGGGATCCAAAAGGAGCTGCAA3550.12589634652348766No Hit
CTCGTGGGGTTTTTGATGTAGGTGTTTATTACTAAATACTTCCCTCTTAAAA3520.12483243373596523No Hit
AATTGCGGGTAGCTAGTTCCTAATGAATATTTTGTTGGAAGCTCTATCCTCT3520.12483243373596523No Hit
AAAACAGGGAGCCAACCAATTCCTCAGTAATTCTGCAGTGAACCAAAAGAGT3490.12376852094844279No Hit
GTAACTGGGATGTCTGCTGCTATCACAAAATACCTGCGACTGGAGAATATAT3480.12341388335260198No Hit
ATGTAAGGGAATGTTGCTATTTAGCAATGTGCTTGGTTTTAAAGAAATTCTC3480.12341388335260198No Hit
TAGCAAGGGGTATCCTGACTAAGGCCAAGGATGATTCAGAATTAGAAGGACA3470.12305924575676117No Hit
ACTACCGGGATGTACACACTTGTTTAATTAGGAGAAGCTATTCCAACCTGAT3470.12305924575676117No Hit
GTTACTGGGAGTCGGAGCCAGCGCGAGCCGCCGCCGCCATCACTGCCGCTGC3450.12234997056507954No Hit
ATCACGGGAGCATGACTTATTACCATTGATAATAACCTTGATTACCTGTCTG3450.12234997056507954No Hit
GTCCTTGGGTCTATTCATTTAGTACATTTTCTTAACCTTGAAAACAGACATT3440.12199533296923873No Hit
ATTGTTGGGGCGGGACAGGGTGGCTGGCACGAGATGTGTGGGACTGGGACGC3440.12199533296923873No Hit
GGTAATGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT3430.12164069537339793No Hit
AAGTTGGGGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAGGAAGCAGC3410.12093142018171629No Hit
TGCTCAGGATTCTGATGGATTCAGCAAATTATTATCATCATGACTTTTTATG3400.12057678258587551No Hit
AGCAATGGGAGGTGTGAGCCACCACGCCCGCCCTAAAAGTGGATTCTTTTTT3390.12022214499003468No Hit
GCACAAGGGAAGTACTGGATATTTAAGTGTGAGTAATGTCTTCATTAGAAAA3380.11986750739419387No Hit
CACACTGGGGTGACAGTGTCTCTTCTCAGGCCTGCAGTGTGTACTATTTGCT3370.11951286979835307No Hit
TTGCACGGGCAGACGCTGGTATGGGGGCTCGGACCCCTCACTGGGGACAGGG3370.11951286979835307No Hit
ATTTCAGGGTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCCGGGTGGTG3370.11951286979835307No Hit
TATCTGGGGGAGTGGTTAAAGTTTTCAAAGCGGTTGGCAGCAGCGGCGCTTG3360.11915823220251226No Hit
ATGTGCGGGCGATCTTAGTCGGGGAACATCCTTATTTTACTTTTCAGTACTG3360.11915823220251226No Hit
GTGCGAGGTAGGCTGAAGTGATTCTCCTGCCTCAGCCTCGCAAGTAGCTGGG3350.11880359460667143No Hit
AAGCATGGGTATTTATAGGGTACATGAGATGTTTTGATACAGGCATACAATG3340.11844895701083064No Hit
GAAGTCGGGTGGGCCTTCTTTATTAATGGTATATGTCTATATCAATGAGTTA3340.11844895701083064No Hit
TATGAGGGGACCCTTGGAAGATAAAGCTGGGTCTTCAGGAACTCAGTGTCTG3330.11809431941498982No Hit
TCATTAGGGAAAGAAAAACCTAAAGAGGATGCATATGAATATAAAAAGCGTT3320.11773968181914901No Hit
AGTCTAGGGGGAAGGAATGTGCTTGCCTGTCAGGTGCAGGGAGAATTCAGTG3320.11773968181914901No Hit
TTCTCTGGTATAGCAAACTGGATTAATAAATAGAAACACATAGTGTAGTCTT3310.11738504422330819No Hit
TACTTAGGGGGTTTGTCCAATATGGCGGCGCCCAGTGGCGGTGTGAACTGTG3300.1170304066274674No Hit
ACTTTTGGGAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA3300.1170304066274674No Hit
TCAACCGGGACTAACAGCTCCACCGAGATTCCTGAATTCCCAATTGCACCTG3300.1170304066274674No Hit
CGACTTGGGGCGTTTCCAGGAGGGAGCGGCCTTTGCTCAGCGCGAGACGGCT3280.11632113143578576No Hit
GACCTCGGGCTGTGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTA3270.11596649383994496No Hit
ACTCGCGGGAATCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTA3270.11596649383994496No Hit
AAAGCTGGGAGATTTGTTGCCGTCATGTCGGCTGCGATTGCAGCTCTGGCCG3260.11561185624410415No Hit
GAATTAGGGCGCTCAGCTCTGTGCTGTGTGAAAGCACCACCAGCCAGTGTGT3250.11525721864826333No Hit
CTTCTAGGGATATTGGATCCTTTTATATTAGTGTTTCTTGGTTTCTCTTTGG3250.11525721864826333No Hit
TAACCTTGGGCATCTAAAGGTGACAGCTTGTGGGCAGAACCATGCACAGGAA3200.1134840306690593No Hit
AAAACAGGGTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTTCATTGTGGACA3180.11277475547737767No Hit
CAGCATGGGACACAGAGATCTCATTCCTTGTGAAAGAATCCTCGTTTTGGAA3180.11277475547737767No Hit
CTGAAAGGGATATCTTACCAATGTATTGTTTTGAAGAGGGTTATTTTGGGGG3170.11242011788153686No Hit
GCTTACGGGAATATCACAGCATAACCCCACCCTTTACATTTTGTGCAGTGAT3170.11242011788153686No Hit
TCTGCCAGGGGGGGGAGTTGATTTGCACTGCTCCGGGTGTCGGCTCCGGCGG3160.11206548028569605No Hit
ATTCTAGGGATGCCAAAACCACACAGTGTTTCACTTAATGATACCGAAACAA3160.11206548028569605No Hit
CACAAAGGTAAGATAATTTTATTGTCTATTTAAAGACAGGCCTTGGCAATTG3160.11206548028569605No Hit
ACGCCGGGACTGCAATCCGGAAAGAAATAATATCACTGCAGAAAGAATGTGA3130.11100156749817361No Hit
TGTCCAGGGACAGAGGCTTAGAAACTGGCGCAAAGTCATTCGTTCATTCAAC3120.11064692990233281No Hit
ATCTACGGGTGCTGACTGCAATGTGATAGAGATAGATGATACCCTCGACGAC3120.11064692990233281No Hit
GTTACAGGGGGGTAAGATTTCTGAACAGATAAGCACTTTTCCAAAGTTAAAT3110.110292292306492No Hit
GTGGTCGGGGGTTTTTCATTGATGATAAACGACTACAGACTTAGTAGTGGAG3110.110292292306492No Hit
GCCTTTGGGTAGAGGGGCTACTGGATGTGGGAAAACCACACAGGTTCCCCAG3100.10993765471065117No Hit
TTTAATGGGACAGACAAGATCTTCAGGCAGATGGACACCAACAATGACGGCA3100.10993765471065117No Hit
GACTCTGGGTTAGTAAAGGAATACTTTCAAGTCATCCCAAATATGGTTCCAT3090.10958301711481039No Hit
CTTCAGGGAGCCAGAGAGCTGTCATTTCAGTACCGGGATTCAGAATTGATCC3070.10887374192312875No Hit
TATGCCGGGGAGTAGAAAACTAAATGCTGTTCATATCACTGGGAAAGCTCAT3060.10851910432728795No Hit
CGCGGGTTACTCAGATGTACACAATTGCTCTTACAATTACAAAGCCGATGCT3060.10851910432728795No Hit
GCCTGAGGGCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTG3050.10816446673144714No Hit
GCTACGGGATTTCTCTAATACTTATTGAAGTTCTGAACCTTGAATTTAATCA3040.10780982913560631No Hit
CACACGGGGTACTTTTTTAGTCGTTTTATTGAACTAGGCTACTAGGTTTAGT3030.10745519153976552No Hit
TTGGCTTAGGGCAGGATTTCAACTTTATGCATATGTATTTTTAATTAAAGTA3030.10745519153976552No Hit
CACAAAGGGAACAGCACTGGGATGTGTTGAGGAGGGGGCAGTTTGCTGTGCT3020.1071005539439247No Hit
AGATTAGGGGGGGCCTACTCACTTATTCTTTCACCCCCTCTCCTCCTGCTCA3020.1071005539439247No Hit
CTTCTTGGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTG3000.10639127875224309No Hit
TTATTTGGGAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGCAAAC2990.10603664115640228No Hit
TCTAGTCGGGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCAT2990.10603664115640228No Hit
TTGTTAGGGCTGTTGCACAGTTGCCCTTGGAGTTGCTGCATCATCTTGATTT2990.10603664115640228No Hit
CAGGTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2980.10568200356056145No Hit
TTAGTTGGGCATCTTGAAGGTGTACACAAGGTCACAAAGCATTGACCAAGAA2980.10568200356056145No Hit
GTCTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2980.10568200356056145No Hit
ACTACGGGAATTGAAAGGCATCGAAGTGAAGGACACTGTTATCCAGGTGACC2980.10568200356056145No Hit
TGTTGTGGGAGGAGAATTTACAATAATTAGTGAAAATTATTCAATTCTCATA2980.10568200356056145No Hit
ACATATGGGAACATGGAGGCATGTGGTATCATGATATTCTTCACTAAATTTA2970.10532736596472066No Hit
AAAGCTGGGCGATCTCTAGTTGGGAAATCAAAGGAACTTGTTTCTTCAAGCT2970.10532736596472066No Hit
GTAGATGGGCATCCATTTGGGCTGAGGGTAGACTGGCTTCACCCTTAGACTT2970.10532736596472066No Hit
ACAGATGGGATGTATGAAGTTATGTGGAAAAATAGGAAAGGATAACAGTTCA2960.10497272836887984No Hit
GTATATGGGTGACAGACAAGTTCTATGTCCATTCTGAGGGAACACTAGATAT2950.10461809077303903No Hit
GTGACTGGGAAAAACAGTTTTGTTTTGTTTTATTTTCTGAACGAAGATAATG2950.10461809077303903No Hit
AAATGCAGAGAAAAAGATGTTACCTTGAAAAATGAAGTAAGAAATCCTAATA2950.10461809077303903No Hit
CAACCGGGTATCCTACTTATGGACTCACTCCGAGGCCCTTCTCGGTCAAATG2950.10461809077303903No Hit
TTCAATGGGAAGGGAAGCCATCGGTACCTTCTCAGGTACTTTGTTTCTGGAT2950.10461809077303903No Hit
CAAAGCGGGGGGCGCGGGGGATTACATTGGATCTGCAGATTCATGCAATCCC2950.10461809077303903No Hit
GCCAAAGGGAGGCAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG2940.10426345317719823No Hit
AAAAATGGGGGACACAGTGAGAAGACAGCTGACTATGAACCTATGAGCTGGA2940.10426345317719823No Hit
GCCAGTGGGGGGGCTAGTGCAAGGGACTATGAATCCCTTGGAGAGGAAATCA2940.10426345317719823No Hit
TCACGAGGTTATGGAAACTCTGAGAAGCTGAATGATACATGCAGGGGATATT2940.10426345317719823No Hit
AGCTTAGGGGATAGCATTTCACTCTGCGTTCTCCTAGATCTGAGCAAGCTGT2940.10426345317719823No Hit
CGGCACGGGATCTTTTGGCGGGAAAAGATAAAGACAATCCTGGTACTTCTTC2920.1035541779855166No Hit
TAAGATGGGGGCTGCTGCCGCTGCCGCCCTGAGTCACTGCCTGCGCAGCTCC2920.1035541779855166No Hit
TCAAGTGGGCCATACAGCCCCAGGCAATGGGCACAGCCTTCCTCCCCAGAGG2910.10319954038967578No Hit
TCTGGCGGGGGGATGAATCCTTCTAATGGTGATTCTCATTTAGAGTAAAAGA2910.10319954038967578No Hit
ATCCTGGGGCATTGAAGAATAACCAAGATGTCATCAGGGCCACTCAGCCCTT2900.10284490279383499No Hit
ATCCGGGACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATGG2900.10284490279383499No Hit
CTGACTGGGAAAAACAACATATATGTTGAAGCTCTTATTAGAATACAAATAT2900.10284490279383499No Hit
GTATTGGGAGATTAGAGTAAGTAGGACTAGAATAATTTTAGTAAATATTCTT2900.10284490279383499No Hit
CACCCAGGCTGGAGTGCAATGGCACGATCTCGGCTCACTGCAACCTCCTTCT2900.10284490279383499No Hit
AAAATTGGGACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTT2900.10284490279383499No Hit
CTTCTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2890.10249026519799417No Hit
TTGCGCGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT2890.10249026519799417No Hit
TTCAACGGGGGACCTAGGCTATCTTACCATGAAGGTTGTTTTGCTTATTGTA2880.10213562760215335No Hit
TTTTATGGGGAGGCTTAGACTTTACCATATAGACAAGGAGAAATATTGAAAG2880.10213562760215335No Hit
GCTCTAGGGCGGATCAGATGGCTATATCTCACTATTTTGCTTTCTGCCTTCA2870.10178099000631255No Hit
GGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC2870.10178099000631255No Hit
AATACAGGGAATTCAGACTAAGATGTTGGTGACTGAAAAGCTGGGGTCAGTA2870.10178099000631255No Hit
TGCTAAGGGTCTCACATAGGGAGCTTTCTCCATGTATACTATTTAGAGGTTT2860.10142635241047174No Hit
TTACCTGGGGACATATACACTTAATATAGTTCTAATAAAAATCCTTCCAAAA2850.10107171481463093No Hit
ACAAGGGGTAATACACAGAATCCTGCAACTTTTTGCACAGCTGGTATCTGTC2840.10071707721879013No Hit
CTTATGGGGCTGCAGAATGGGCTGTTGGAAGGTATAATAGCTTATCGAACCA2840.10071707721879013No Hit
ACGTTTGGGTCCAGCCACTAGATTGTCCCTCTCTGGGAAGCCCTAAACCAAC2830.1003624396229493No Hit
TTCATCGGGGACTGGACAGTGGGGTTTCTACCATGAATGAAATGAAGAGTTA2830.1003624396229493No Hit
TTGTGTGGGGGTTGCACACCAGAGGACGCACTCAGGGGAGAGGCCGTATGTG2830.1003624396229493No Hit
AATTTTGGGTATTCTGGTGCTGCTTCATAACAAAAATGAAAAGCCATGCATC2820.10000780202710849No Hit
ATGCAAGGGGGGCGCTCGAGGGACCATGGCCGATCCTCGCGTGAGACAGATC2820.10000780202710849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)