FastQCFastQC Report
Sat 31 Dec 2022
SRR5844196.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844196.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119090
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC5540.4651943907968763No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT5380.4517591737341506No Hit
ATGGCTGGGCATTTTTTGTGATCTTTATTTCCAGTGATGAGCACTGCACCTA2180.18305483247963725No Hit
CCAACCGGGATTCATTGTTTGTTTTGGCCAAATAAAAATGCTTGTATTTCTT2070.17381812074901334No Hit
TACAGCGGGGAAATTATATTGCATTACTATTTGCAGTCAAACTTTGATCCTT1900.15954320261986735No Hit
ATAAGCGGGGTGGCCAGTTTTCGTAATGAAACAGTCTTTAGTAATTTAATCT1730.1452682844907213No Hit
CTCGAGGGGACAAGCTGAAGCACAAGCATGGCCTTGTGGAGCGGGCGATGGA1720.14442858342430095No Hit
TACATCGGGGAAGCTCAAAGAGACACCTGGACCCCCATGTTCATTGCAGCGT1720.14442858342430095No Hit
CACGTGGGGAGATTGGGGCATATTTCTGATAAAGACCCACCTCTAGTGAGAT1690.14190948022503988No Hit
TCAAGGTGGGGCATTGGGATGGGTGGAGAAAGACGACAAGAGAGCAGAGAAT1680.14106977915861954No Hit
CACTTAGGGATTGACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAG1660.13939037702577883No Hit
AGAAATGGGAAGGCTGGAGATGGTCCCAGATCCAAGATGTCCCAAAAGCCTT1650.13855067595935847No Hit
GAAACAGGGAACTAGAGATTTATTGTACAGCATGGTGACTACAGATAAAAAG1650.13855067595935847No Hit
TGCGCACGGGGTAACGAGTACATAGTTTCTGTTTGGGATGAACAAATTCTGG1650.13855067595935847No Hit
TTTCCAGGGTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGT1630.13687127382651776No Hit
TCAACAGGGGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAAC1630.13687127382651776No Hit
AGTCGCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1630.13687127382651776No Hit
AGCTTGGGATATTCCACTTGGGAGGGTCAGGCTGTGGCCTTCTGGAGCAGGT1620.1360315727600974No Hit
CCGTGCGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1620.1360315727600974No Hit
CACAAAGGGTTTCGATTATAAATGAATGATCACCTCGAAGTCACTTTAGAAC1620.1360315727600974No Hit
GACACAGGGTACAAAGGTGAACTCGTTTCACCTCATCGACAGTGTAATCTAG1590.13351246956083634No Hit
CTTTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.132672768494416No Hit
TCTGTTGGGACTGGAGGAAATGAATGCAGTTATTTGCTGCCAGAGGCCAGAG1560.13099336636157527No Hit
TGTGAATGGGGTGATTAAAAATATGACAGAAATAATCTTACCAGAATATACA1560.13099336636157527No Hit
GTGCATGGGAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCTGTTGTGGAG1540.12931396422873456No Hit
TTGTGTAGGGCATGTCAGCATAAATGAGAAAAAGAAGTGGATCATGCAGCTA1530.12847426316231422No Hit
GAGCAGGGTGCCCTCATAGTGTAATGGCACCAGCAGATCTGCCTGTGCACAG1510.12679486102947352No Hit
GCTCAAGGGAGTTTAGATCTAGAAGCTGGTAAAAATGACAATATCAATCACA1500.12595515996305315No Hit
TCAACTGGGGGCGGCCTTTCACGCCGGGATCCGGGTCCCGGTGCTTCTCGGT1500.12595515996305315No Hit
ATAAGCGGGTAAGAAACATTGGAAGCAGGTTAAATGTTTTGTAAACTTTGAA1490.12511545889663278No Hit
AATCTGGGTGCTTGTTCAGATAAAAAACTAAAGCTAAAATGGTGTGTAATTT1470.12343605676379209No Hit
CTCACGTAGGGAGTGAAGCCAGGCGACGAGAACCTTCGGGAGATGAACAAGA1470.12343605676379209No Hit
ACCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATA1470.12343605676379209No Hit
CTCCCTGGGTGGCTATTCCTAGAAACACACAACCAACCAAGACTGAATCATA1460.12259635569737175No Hit
TGTGCATGGGTATTAGAGTTCATGGAACATAGAACAACCATGAATGAGTGGC1460.12259635569737175No Hit
AATCTCGGGCTTTAAACAGTTGTTCAATACCACCATTGAAACTTCTGTAAAG1450.1217566546309514No Hit
CATCAAGGGCTCTGCATGCCCATGAATGACTGCAAAAATGCCACCAATATTG1450.1217566546309514No Hit
CTAGCCAAGGGGATGCGCTCTGCCCTGGCAGCGGCCCTGTGCAGATCCCTGA1440.12091695356453101No Hit
CAACTTGGGCAGGATACAGATTCTTTTCAGTCAAACATGTTCATTCACCAAG1430.12007725249811067No Hit
TTTGCGGGGAGCCTGCATCTTTTGTATAGTAAGGAATGATCTAGTTAGAGCT1430.12007725249811067No Hit
GTACAAGGGATCTGACAGAGGGGGGACAACATGGCGGCCTTCATGCTGGGCT1430.12007725249811067No Hit
CTAAGTGGGCAAGCCTGCAGGACCGAGGGCCACAGCCGGACAGGGGGTAGCC1430.12007725249811067No Hit
CCAAACGGGCTATTTAGACTGTATAGACTGATTTAGATTACATACTTTAGAG1430.12007725249811067No Hit
ACAGACGGGGGGTACAGGCCTGGAGCTAGGGGACGAGGACTGTTCTCCTCCA1420.11923755143169032No Hit
CTATTCAGGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTATACCTACT1420.11923755143169032No Hit
AACTGGGATAGTCCTCAGGGCTCAGTCTTTTGAGGTAAGTGGAATTAGAGGG1410.11839785036526997No Hit
GATTTGGGACAGGGACTAGGACATCGACCACCCTCTGCCCCTCCTGCAGTCA1410.11839785036526997No Hit
GAGCGGGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC1410.11839785036526997No Hit
TAAATAGGGTTACTTTGGCTTCCTTATACACTGTAGGGACAAAAGGAAAAAC1400.11755814929884961No Hit
TTAACTGGGGGCGAGCGGGGCCGCGCGCGCACGGGACCCGGAGCCGAGGGCC1400.11755814929884961No Hit
TCAAGAGGGGTGTACAATTCAGTATTATTGGATATATTCATAATGTTGTGCA1400.11755814929884961No Hit
GGATAAGGGGCTGTTAACAACACATTAAAAATCAGTTTGCTATTTTTGATGT1400.11755814929884961No Hit
GCATTTGGGATGGTGCAGCTTTTTTTTAATTATGTTTTTAAAATTATACAGT1400.11755814929884961No Hit
TTCATGGGTTTCTACTGCTTTGACTTTTATTTCTCAAAAAGACTGTTTTGTT1390.11671844823242926No Hit
CTGTGGGGGGGAGGACCAGCCGAAAATTGCCAAACGACTTTCTGACCTCAGT1390.11671844823242926No Hit
GTGCTGGGGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAG1390.11671844823242926No Hit
TCTGTAGGGGTGTGTCTGTTTTAACAATGATGAATGGTGGGGTGAAGGAAAC1390.11671844823242926No Hit
GAAATGGGATGCAAAGTTCTGCAGAAGAAGGTAAGATGGAGCTTCATCTTCA1380.11587874716600889No Hit
TATTCTGGGCTGCAAAGGAATCTGCTCTTTGAGGTTTCTTTCTGCACTAGAG1370.11503904609958854No Hit
TTCAAGGGGGGGAGCGAGGAGGCGAGAATGGACCAATGGTGCCACGGAGCAC1370.11503904609958854No Hit
TAATACGGGGTGTTGGAATTGGGTGAATGACTGGCTGCTCCTTGATATCCTA1360.11419934503316818No Hit
ATTAATGGGAAGTTACATCTCTGACAGAGTATTATCACTGATATAAAAGGAA1360.11419934503316818No Hit
ACAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1360.11419934503316818No Hit
TGATCTGGGATGATAATTACCTTAAGCTTTCTTTATCTAAAATGTAGAACAT1350.11335964396674783No Hit
GCTTAGGGGGGGGAGAAAGAACGTGAAATGGTTTGTGTATTATTGAATTTTA1350.11335964396674783No Hit
ACAGTAGGGGTCCTGGACGTGGGAACGGGCAGGGATTTCATGACAGGGACCC1350.11335964396674783No Hit
TACATGGGATCATTAAAAGACTGCACAAAACCACACCTTGCACAAAGGCCAT1350.11335964396674783No Hit
AGTGATTGGGGTGGTCCCAGCTACTTGGGAGGTTGAGGTAGGAGGACCACTT1340.11251994290032749No Hit
TTCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1330.11168024183390714No Hit
GCGACAGGATACCAGTTATATGAATTGACTTAAGTATCTTGAAAAAGAAACT1330.11168024183390714No Hit
TTAAGGGGGTGACATATCTAAACATAAGAACTCAAACCATCAGACTTTTAGA1330.11168024183390714No Hit
GTGCGAGGGGGGCTTTGGAAACAAATCCTGGCATTGTACCATTTATCTGTAA1330.11168024183390714No Hit
AATCCCGGGTTCAAGCGATTCTCCTGTCTCAGCCTCGTGAGTAGATGGGACT1320.11084054076748678No Hit
CTTCCTGGGCAAGTCTAAGCCTTGCTTTTATCTTAGTTGTCTGGGTGGTAGC1310.11000083970106642No Hit
TCGCTAGGGCAATTGACAAAATAGGGCAGGCAGCTCATCTCATGTCCTGAAG1300.10916113863464606No Hit
GGGCATGGGAATGTAAATCAGTTACTTAGTCATTGACTGTGACAGATGACAG1300.10916113863464606No Hit
CGTGAAGGGAGCAACCTTTCTAAAAAAGCAAACCTTTTTCCTGGGAGGAAAA1300.10916113863464606No Hit
CGATTTGGGTAGCTTTTCTCAAGCAACATTGGAACAGGTTTTTGTAGAACTC1300.10916113863464606No Hit
TAACATGGGGAGATGCGCCACGGTTTCGGTAGCGACGGTAGCTCTAGCCGGG1300.10916113863464606No Hit
ACATATGGGTGGTTGAGCAAAAACAGGCTTGAGAAGCCAGGAGCGCACAGAG1290.10832143756822571No Hit
TGGGCGAGGGCTTTGGATAATATTATCATTTTAGCTATATTAATTCTTCCAA1280.10748173650180536No Hit
TTGAATGGGGCTCTTTTCCGGTTAGCGCGGCGTGAGAAGCCATGAGCAGCAA1280.10748173650180536No Hit
GTAACCGGGAAAACTAGAAAGGGACTTTTTTTCCTTTCTTTTCATAATGAAG1280.10748173650180536No Hit
AGTTAGGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTG1280.10748173650180536No Hit
CGTTCGGGATGACCCAATACCTGCCCTTGGCAATCAGAATGTAGTGCAAGCC1270.106642035435385No Hit
CGTGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.106642035435385No Hit
ACTTATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTTACATC1270.106642035435385No Hit
TTATTGGGGGGAGCTAGAAAGTAGCTGAGCTGGTGCAGTACCTGCTGGTTAA1260.10580233436896466No Hit
GAAACTGGGGAAGCAGAATACGCTGCCATCAACTCCATGCTGGACCAGATCA1260.10580233436896466No Hit
AGAAAAGGGCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTTGATTC1260.10580233436896466No Hit
GTCAAGGGGCTAAAGAAGTAGCTGATTGTTTCAAGAAAACATTTGAAGAATG1260.10580233436896466No Hit
GATTAAGGGATTGACATATTTTACCATTACTTCCACCTTACTATAATTGTTT1260.10580233436896466No Hit
TAAACAGGGATGGCTTTGGTTGCTGCTATCCTTTTGCTAGTGATTATCATTC1250.10496263330254428No Hit
TTTTGAGGGGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACTTTT1250.10496263330254428No Hit
AGAGGTGGGATCTAGTCGCGGGGATCTGAAAACGGGTAATACTGGCCTCATA1250.10496263330254428No Hit
CAGACAGGGTAGCAGAAATTTTGAAGCCAGAAGGACAACATATGAAGCTTAG1250.10496263330254428No Hit
TCATCAGGGATTGTGAGTTTATAATCATCTCAAGTTATGTAATAAGTTAGGA1250.10496263330254428No Hit
ATTAGGGGGGATCTCTGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAG1250.10496263330254428No Hit
AGTTTTGGGTCATTTTACTGGTTTTGATAATGCATTATAGTTGTGTAAGACA1250.10496263330254428No Hit
ATATAAGGGAAGGAGGATCGGGTCATAAGCAATCCCAAACTACCAACAAGAG1240.10412293223612393No Hit
GATTCTGGGACCACTTGTATTGCAGATGTTTTTTTCACCTTGTAGTGTATAT1240.10412293223612393No Hit
AACTCAGGGCTACCAAAGAGAGGGAGTTGACTGGCTTTTAAAAAGTATTTAA1220.10244353010328323No Hit
GTAACAGGGGTAAAGGCCACATTTCATTTAAACATAAAAGGTACGGTGGCTC1220.10244353010328323No Hit
TTTCCTGGGTTACGAAGTTCCAGCCAGGCCTTTACTTTCCCAATCCTATTTC1220.10244353010328323No Hit
AGATTCGGGTCTCAGGAAGGGCATTCTTGCTGCATCAGTCAGCACTGTGACT1220.10244353010328323No Hit
GTATCAGGGGCTCTTCCGATCTCGGAGTGGGGGGAACACTTCTCAACACAAG1220.10244353010328323No Hit
TTCAGCGGGGAATCTTGTCTCAATGCTCATAACGGATCAGAAATACAGATTT1210.10160382903686288No Hit
TACGCAGGGAAATTAATGATTTATTAATATACATGTTTCTATGAATTTCCAT1210.10160382903686288No Hit
GCAAATGGGTTTCTTAGTGAGCTGGATTATCTTGAACCTGTGCTATTAAATG1200.10076412797044253No Hit
GTATGGGGTGACTACCCCCAGAATGAGCTTTGATGGTCTCTACTGGGCCTTT1200.10076412797044253No Hit
GAAACGGGGAAGAGCAAAACTCTGTCTCAAAAACAAACAAACAAACAAACAA1200.10076412797044253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)