FastQCFastQC Report
Sat 31 Dec 2022
SRR5844195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118846
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC5560.46783232081853826No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT4990.4198710936842637No Hit
ATGGCTGGGCATTTTTTGTGATCTTTATTTCCAGTGATGAGCACTGCACCTA2460.20699055921107987No Hit
CTCGAGGGGACAAGCTGAAGCACAAGCATGGCCTTGTGGAGCGGGCGATGGA1990.16744358245123941No Hit
ATAAGCGGGGTGGCCAGTTTTCGTAATGAAACAGTCTTTAGTAATTTAATCT1890.15902933207680528No Hit
CCAACCGGGATTCATTGTTTGTTTTGGCCAAATAAAAATGCTTGTATTTCTT1850.15566363192703161No Hit
GACACAGGGTACAAAGGTGAACTCGTTTCACCTCATCGACAGTGTAATCTAG1830.15398078185214478No Hit
GCTCAAGGGAGTTTAGATCTAGAAGCTGGTAAAAATGACAATATCAATCACA1760.1480908065900409No Hit
TGTGAATGGGGTGATTAAAAATATGACAGAAATAATCTTACCAGAATATACA1750.14724938155259748No Hit
ACAGACGGGGGGTACAGGCCTGGAGCTAGGGGACGAGGACTGTTCTCCTCCA1690.14220083132793698No Hit
TGCGCACGGGGTAACGAGTACATAGTTTCTGTTTGGGATGAACAAATTCTGG1690.14220083132793698No Hit
GAGCGGGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC1680.1413594062904936No Hit
AACTGGGATAGTCCTCAGGGCTCAGTCTTTTGAGGTAAGTGGAATTAGAGGG1630.1371522811032765No Hit
GGATAAGGGGCTGTTAACAACACATTAAAAATCAGTTTGCTATTTTTGATGT1630.1371522811032765No Hit
AATCTGGGTGCTTGTTCAGATAAAAAACTAAAGCTAAAATGGTGTGTAATTT1620.1363108560658331No Hit
AGATTCGGGTCTCAGGAAGGGCATTCTTGCTGCATCAGTCAGCACTGTGACT1610.13546943102838968No Hit
TACATCGGGGAAGCTCAAAGAGACACCTGGACCCCCATGTTCATTGCAGCGT1610.13546943102838968No Hit
CACTTAGGGATTGACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAG1600.13462800599094626No Hit
ACAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1590.13378658095350285No Hit
CTCCCTGGGTGGCTATTCCTAGAAACACACAACCAACCAAGACTGAATCATA1580.13294515591605943No Hit
CACGTGGGGAGATTGGGGCATATTTCTGATAAAGACCCACCTCTAGTGAGAT1570.13210373087861602No Hit
ATAAGCGGGTAAGAAACATTGGAAGCAGGTTAAATGTTTTGTAAACTTTGAA1570.13210373087861602No Hit
GAAACAGGGAACTAGAGATTTATTGTACAGCATGGTGACTACAGATAAAAAG1550.13042088080372918No Hit
TTTCCAGGGTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGT1540.12957945576628577No Hit
TAAATAGGGTTACTTTGGCTTCCTTATACACTGTAGGGACAAAAGGAAAAAC1530.12873803072884238No Hit
GTCAAGGGGCTAAAGAAGTAGCTGATTGTTTCAAGAAAACATTTGAAGAATG1530.12873803072884238No Hit
CTAAGTGGGCAAGCCTGCAGGACCGAGGGCCACAGCCGGACAGGGGGTAGCC1520.12789660569139893No Hit
CACAAAGGGTTTCGATTATAAATGAATGATCACCTCGAAGTCACTTTAGAAC1520.12789660569139893No Hit
CCAAACGGGCTATTTAGACTGTATAGACTGATTTAGATTACATACTTTAGAG1520.12789660569139893No Hit
TCAACAGGGGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAAC1510.12705518065395555No Hit
TAATACGGGGTGTTGGAATTGGGTGAATGACTGGCTGCTCCTTGATATCCTA1500.12621375561651213No Hit
AGCTTGGGATATTCCACTTGGGAGGGTCAGGCTGTGGCCTTCTGGAGCAGGT1500.12621375561651213No Hit
TGTGCATGGGTATTAGAGTTCATGGAACATAGAACAACCATGAATGAGTGGC1490.1253723305790687No Hit
CGTGAAGGGAGCAACCTTTCTAAAAAAGCAAACCTTTTTCCTGGGAGGAAAA1490.1253723305790687No Hit
GATCTCGGGCAACCAATCCTAAATCTCATAACTTTTATTTGATCATTTAGAG1470.1236894805041819No Hit
CTAGCCAAGGGGATGCGCTCTGCCCTGGCAGCGGCCCTGTGCAGATCCCTGA1470.1236894805041819No Hit
CTCACGTAGGGAGTGAAGCCAGGCGACGAGAACCTTCGGGAGATGAACAAGA1450.12200663042929505No Hit
GTGCATGGGAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCTGTTGTGGAG1450.12200663042929505No Hit
CTTCTGCGGGGAGAGCACATCCGGTGTTAGAAGCGCTGGTAGGCCTTGGAGA1450.12200663042929505No Hit
CCGTGCGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1450.12200663042929505No Hit
TTATTGGGGGGAGCTAGAAAGTAGCTGAGCTGGTGCAGTACCTGCTGGTTAA1440.12116520539185165No Hit
TAACATGGGGAGATGCGCCACGGTTTCGGTAGCGACGGTAGCTCTAGCCGGG1440.12116520539185165No Hit
TACGCAGGGAAATTAATGATTTATTAATATACATGTTTCTATGAATTTCCAT1440.12116520539185165No Hit
CAACTTGGGCAGGATACAGATTCTTTTCAGTCAAACATGTTCATTCACCAAG1430.12032378035440822No Hit
GTACAAGGGATCTGACAGAGGGGGGACAACATGGCGGCCTTCATGCTGGGCT1430.12032378035440822No Hit
TCAAGAGGGGTGTACAATTCAGTATTATTGGATATATTCATAATGTTGTGCA1430.12032378035440822No Hit
CTGTGGGGGGGAGGACCAGCCGAAAATTGCCAAACGACTTTCTGACCTCAGT1430.12032378035440822No Hit
GTGCTGGGGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAG1420.11948235531696481No Hit
GCTTAGGGGGGGGAGAAAGAACGTGAAATGGTTTGTGTATTATTGAATTTTA1410.11864093027952141No Hit
TACAGCGGGGAAATTATATTGCATTACTATTTGCAGTCAAACTTTGATCCTT1410.11864093027952141No Hit
GTATCTGGGTTAGAAAAATATTAAAATTGAGCCTGATGTTGTCTAGGTAAGA1410.11864093027952141No Hit
AGTCGCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1400.11779950524207798No Hit
CTTCCTGGGCAAGTCTAAGCCTTGCTTTTATCTTAGTTGTCTGGGTGGTAGC1400.11779950524207798No Hit
CATCAAGGGCTCTGCATGCCCATGAATGACTGCAAAAATGCCACCAATATTG1400.11779950524207798No Hit
TCTTGCGGGAATTGTAGAGTTATTAAAAGCTGGTGCTAAGGTTAATTGTGAA1380.11611665516719116No Hit
CTTTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1380.11611665516719116No Hit
ACTTATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTTACATC1370.11527523012974773No Hit
GTAACCGGGAAAACTAGAAAGGGACTTTTTTTCCTTTCTTTTCATAATGAAG1360.11443380509230433No Hit
ACATATGGGTGGTTGAGCAAAAACAGGCTTGAGAAGCCAGGAGCGCACAGAG1360.11443380509230433No Hit
AGAAATGGGAAGGCTGGAGATGGTCCCAGATCCAAGATGTCCCAAAAGCCTT1350.11359238005486093No Hit
AATCCCGGGTTCAAGCGATTCTCCTGTCTCAGCCTCGTGAGTAGATGGGACT1350.11359238005486093No Hit
TTTCCTGGGTTACGAAGTTCCAGCCAGGCCTTTACTTTCCCAATCCTATTTC1340.1127509550174175No Hit
ATTAATGGGAAGTTACATCTCTGACAGAGTATTATCACTGATATAAAAGGAA1330.11190952997997408No Hit
CTCTTGCGGGGAATGTTTGTGAACTACTGCTCAATAAATATCCAATAAATGT1330.11190952997997408No Hit
GCATTTGGGATGGTGCAGCTTTTTTTTAATTATGTTTTTAAAATTATACAGT1320.11106810494253067No Hit
GGGCATGGGAATGTAAATCAGTTACTTAGTCATTGACTGTGACAGATGACAG1310.11022667990508725No Hit
TTCAGTGGGGTTTTTTTAAATTGGTAAAATACTGTACATTCTTTTTATTGTA1310.11022667990508725No Hit
CTATTCAGGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTATACCTACT1300.10938525486764385No Hit
CAGACAGGGTAGCAGAAATTTTGAAGCCAGAAGGACAACATATGAAGCTTAG1300.10938525486764385No Hit
TCAAGGTGGGGCATTGGGATGGGTGGAGAAAGACGACAAGAGAGCAGAGAAT1300.10938525486764385No Hit
TTTCTAGGGCTGAGGGATTTTAGCCTCTTCCCTCTGATTTTTCCTTTCGCGA1290.10854382983020042No Hit
TGATCTGGGATGATAATTACCTTAAGCTTTCTTTATCTAAAATGTAGAACAT1280.10770240479275701No Hit
GATTTGGGGTATCTTTTCTAATGATCAAAAGAGTGTGACTTCTCATTTGTGA1270.1068609797553136No Hit
CGTGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.1068609797553136No Hit
TTATATGGGGACGCTCTTCCGATCTTAAAGCAGGGTCTAGGAAGAATTTACT1270.1068609797553136No Hit
TATTCTGGGCTGCAAAGGAATCTGCTCTTTGAGGTTTCTTTCTGCACTAGAG1260.10601955471787018No Hit
TTCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1260.10601955471787018No Hit
TTTGCGGGGAGCCTGCATCTTTTGTATAGTAAGGAATGATCTAGTTAGAGCT1260.10601955471787018No Hit
ACCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATA1260.10601955471787018No Hit
ATTAGGGGGGATCTCTGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAG1260.10601955471787018No Hit
GATTAAGGGATTGACATATTTTACCATTACTTCCACCTTACTATAATTGTTT1260.10601955471787018No Hit
GAATCCGGGGCCTCCCTTCTTCCGAGCCTCTCCTCTGGCCGCCGCGCGGGAG1250.10517812968042677No Hit
GTATCAGGGGCTCTTCCGATCTCGGAGTGGGGGGAACACTTCTCAACACAAG1250.10517812968042677No Hit
TACACGCGGGACTCTTGGCAGGACACAAGCGCCATAACCTAAGAGGATGTGA1240.10433670464298336No Hit
ATTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.10433670464298336No Hit
AATCACTGGGAAGTAATTCAACGCACGCACTTAACAGAAATGTTAAACTATA1240.10433670464298336No Hit
AACTTTGGGGAAGTATGTTTATCCTATCTGCACATAGTATAAAGTAAAATCT1240.10433670464298336No Hit
TAAACAGGGATGGCTTTGGTTGCTGCTATCCTTTTGCTAGTGATTATCATTC1240.10433670464298336No Hit
TAGTAGGGGTGGGGCATCTGGTCATCCATCTTTCTGTCTCCTTCCTGCTGAG1240.10433670464298336No Hit
GTAAGCGGGCGCTCTTCCGATCTGAGTTCGGGAATTCAATGGAAATACACTT1240.10433670464298336No Hit
TGACCTGGGACATGAGCGAACTCATGCAGGGGTCAACCCTTATAAATGCAGT1230.10349527960553993No Hit
TTGTGTAGGGCATGTCAGCATAAATGAGAAAAAGAAGTGGATCATGCAGCTA1230.10349527960553993No Hit
GTAACAGGGGTAAAGGCCACATTTCATTTAAACATAAAAGGTACGGTGGCTC1230.10349527960553993No Hit
CGTTCGGGATGACCCAATACCTGCCCTTGGCAATCAGAATGTAGTGCAAGCC1230.10349527960553993No Hit
CCTTTTGGGCAGCTTGCCCCACAACACTGGGTGTTGGAGAAAGGGAGAGATA1230.10349527960553993No Hit
GGCTGGGGGAGCCAATAGAGTAAAAGTATAATGGTGAGGGTTATGCTGGATG1230.10349527960553993No Hit
GTGCGAGGGGGGCTTTGGAAACAAATCCTGGCATTGTACCATTTATCTGTAA1230.10349527960553993No Hit
TCTGTAGGGGTGTGTCTGTTTTAACAATGATGAATGGTGGGGTGAAGGAAAC1230.10349527960553993No Hit
TCTGTTGGGACTGGAGGAAATGAATGCAGTTATTTGCTGCCAGAGGCCAGAG1220.10265385456809653No Hit
GCAAATGGGTTTCTTAGTGAGCTGGATTATCTTGAACCTGTGCTATTAAATG1220.10265385456809653No Hit
AGCTGTGGGGAGTTTGGAGTTTGTGAAGTTCGTGACCTCAAACCCAATGGGG1220.10265385456809653No Hit
AGTGATTGGGGTGGTCCCAGCTACTTGGGAGGTTGAGGTAGGAGGACCACTT1210.10181242953065311No Hit
ACATGAGGGCAGAATACTCCATCCACGGCCTCTTCTGTCTGATGTTTCTGTG1210.10181242953065311No Hit
TTCAAGGGGGGGAGCGAGGAGGCGAGAATGGACCAATGGTGCCACGGAGCAC1210.10181242953065311No Hit
AGTGCAGGGCAGAGGCAGGGAAGATTGGAGGAAGCAGGGAATAAAGAGAGGT1210.10181242953065311No Hit
TTTTGAGGGGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACTTTT1210.10181242953065311No Hit
TTCATGGGTTTCTACTGCTTTGACTTTTATTTCTCAAAAAGACTGTTTTGTT1210.10181242953065311No Hit
TACAGTCGGGAAATTAATGTTTAAAGGTAGTTTTGTTCTTACAGAAAGTGTT1210.10181242953065311No Hit
ATTTGTGGGTCACATGGGTTCTTTAGAAGGAACATAGTCAAGTGTGATGGAT1200.1009710044932097No Hit
AACTCAGGGCTACCAAAGAGAGGGAGTTGACTGGCTTTTAAAAAGTATTTAA1200.1009710044932097No Hit
TTGAATGGGGCTCTTTTCCGGTTAGCGCGGCGTGAGAAGCCATGAGCAGCAA1200.1009710044932097No Hit
TCAACTGGGGGCGGCCTTTCACGCCGGGATCCGGGTCCCGGTGCTTCTCGGT1200.1009710044932097No Hit
TCAGCACGGGGTCTGCCATTCAGCCTTTGCCAAAAAATTTGGCACTGATCTG1200.1009710044932097No Hit
GATTTGGGACAGGGACTAGGACATCGACCACCCTCTGCCCCTCCTGCAGTCA1200.1009710044932097No Hit
GCGACAGGATACCAGTTATATGAATTGACTTAAGTATCTTGAAAAAGAAACT1190.10012957945576628No Hit
TCTACAGGGAAACATTATCTTCAAGGATGATTTCCTTTCAATGGATGGCTTT1190.10012957945576628No Hit
AGAAAAGGGCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTTGATTC1190.10012957945576628No Hit
CATAACGGGCAGAATATATTCTCTGAAAACTGTGTGAATTATGAGAAGAAAA1190.10012957945576628No Hit
TTCAGCGGGGAATCTTGTCTCAATGCTCATAACGGATCAGAAATACAGATTT1190.10012957945576628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)