FastQCFastQC Report
Sat 31 Dec 2022
SRR5844194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116505
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGCCGTCT5380.4617827561048882No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGACATCTCGTATGC5320.45663276254238017No Hit
ATGGCTGGGCATTTTTTGTGATCTTTATTTCCAGTGATGAGCACTGCACCTA1940.1665164585210935No Hit
TGTGAATGGGGTGATTAAAAATATGACAGAAATAATCTTACCAGAATATACA1820.15621647139607742No Hit
CTCGAGGGGACAAGCTGAAGCACAAGCATGGCCTTGTGGAGCGGGCGATGGA1800.1544998068752414No Hit
GACACAGGGTACAAAGGTGAACTCGTTTCACCTCATCGACAGTGTAATCTAG1740.14934981331273336No Hit
AGAAATGGGAAGGCTGGAGATGGTCCCAGATCCAAGATGTCCCAAAAGCCTT1730.14849148105231535No Hit
CCAACCGGGATTCATTGTTTGTTTTGGCCAAATAAAAATGCTTGTATTTCTT1660.1424831552293893No Hit
GGATAAGGGGCTGTTAACAACACATTAAAAATCAGTTTGCTATTTTTGATGT1640.1407664907085533No Hit
ATAAGCGGGGTGGCCAGTTTTCGTAATGAAACAGTCTTTAGTAATTTAATCT1630.13990815844813528No Hit
ATTAATGGGAAGTTACATCTCTGACAGAGTATTATCACTGATATAAAAGGAA1620.13904982618771727No Hit
GTGCATGGGAGAGTGAGACAAAGCGGAGAACGCTGGTGGGCCTGTTGTGGAG1620.13904982618771727No Hit
CTGTGGGGGGGAGGACCAGCCGAAAATTGCCAAACGACTTTCTGACCTCAGT1620.13904982618771727No Hit
AACTGGGATAGTCCTCAGGGCTCAGTCTTTTGAGGTAAGTGGAATTAGAGGG1610.13819149392729926No Hit
CACTTAGGGATTGACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAG1590.13647482940646324No Hit
TACAGCGGGGAAATTATATTGCATTACTATTTGCAGTCAAACTTTGATCCTT1550.13304150036479123No Hit
GGGCATGGGAATGTAAATCAGTTACTTAGTCATTGACTGTGACAGATGACAG1550.13304150036479123No Hit
TAATACGGGGTGTTGGAATTGGGTGAATGACTGGCTGCTCCTTGATATCCTA1540.13218316810437322No Hit
TGCGCACGGGGTAACGAGTACATAGTTTCTGTTTGGGATGAACAAATTCTGG1530.13132483584395518No Hit
AATCTGGGTGCTTGTTCAGATAAAAAACTAAAGCTAAAATGGTGTGTAATTT1510.12960817132311916No Hit
TTTCCAGGGTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGT1510.12960817132311916No Hit
CTATTCAGGGGATTTCATTTTGCAGGCATGTCTCTGAGCTTCTATACCTACT1500.12874983906270115No Hit
GCTCAAGGGAGTTTAGATCTAGAAGCTGGTAAAAATGACAATATCAATCACA1500.12874983906270115No Hit
GAAACGGGGAAGAGCAAAACTCTGTCTCAAAAACAAACAAACAAACAAACAA1480.12703317454186513No Hit
CACGTGGGGAGATTGGGGCATATTTCTGATAAAGACCCACCTCTAGTGAGAT1470.12617484228144715No Hit
TGATCTGGGATGATAATTACCTTAAGCTTTCTTTATCTAAAATGTAGAACAT1470.12617484228144715No Hit
ACAGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1470.12617484228144715No Hit
TCAAGGTGGGGCATTGGGATGGGTGGAGAAAGACGACAAGAGAGCAGAGAAT1470.12617484228144715No Hit
CAGACAGGGTAGCAGAAATTTTGAAGCCAGAAGGACAACATATGAAGCTTAG1460.12531651002102914No Hit
AGCTTGGGATATTCCACTTGGGAGGGTCAGGCTGTGGCCTTCTGGAGCAGGT1460.12531651002102914No Hit
GTGCGAGGGGGGCTTTGGAAACAAATCCTGGCATTGTACCATTTATCTGTAA1450.12445817776061113No Hit
TACATCGGGGAAGCTCAAAGAGACACCTGGACCCCCATGTTCATTGCAGCGT1430.12274151323977511No Hit
GTAAGCGGGCGCTCTTCCGATCTGAGTTCGGGAATTCAATGGAAATACACTT1430.12274151323977511No Hit
CCGTGCGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1420.1218831809793571No Hit
CTAAGTGGGCAAGCCTGCAGGACCGAGGGCCACAGCCGGACAGGGGGTAGCC1410.1210248487189391No Hit
TCGCTAGGGCAATTGACAAAATAGGGCAGGCAGCTCATCTCATGTCCTGAAG1400.12016651645852108No Hit
TAAATAGGGTTACTTTGGCTTCCTTATACACTGTAGGGACAAAAGGAAAAAC1400.12016651645852108No Hit
TTATTGGGGGGAGCTAGAAAGTAGCTGAGCTGGTGCAGTACCTGCTGGTTAA1400.12016651645852108No Hit
TACGCAGGGAAATTAATGATTTATTAATATACATGTTTCTATGAATTTCCAT1400.12016651645852108No Hit
CTTTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1390.11930818419810309No Hit
TAAACAGGGATGGCTTTGGTTGCTGCTATCCTTTTGCTAGTGATTATCATTC1390.11930818419810309No Hit
TCATCAGGGATTGTGAGTTTATAATCATCTCAAGTTATGTAATAAGTTAGGA1390.11930818419810309No Hit
AGTGATTGGGGTGGTCCCAGCTACTTGGGAGGTTGAGGTAGGAGGACCACTT1390.11930818419810309No Hit
CGTTCGGGATGACCCAATACCTGCCCTTGGCAATCAGAATGTAGTGCAAGCC1390.11930818419810309No Hit
TAACATGGGGAGATGCGCCACGGTTTCGGTAGCGACGGTAGCTCTAGCCGGG1390.11930818419810309No Hit
GAGCGGGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC1390.11930818419810309No Hit
TTGTGTAGGGCATGTCAGCATAAATGAGAAAAAGAAGTGGATCATGCAGCTA1380.11844985193768508No Hit
TTGAATGGGGCTCTTTTCCGGTTAGCGCGGCGTGAGAAGCCATGAGCAGCAA1380.11844985193768508No Hit
ATAAGCGGGTAAGAAACATTGGAAGCAGGTTAAATGTTTTGTAAACTTTGAA1370.11759151967726707No Hit
AATCTCGGGCTTTAAACAGTTGTTCAATACCACCATTGAAACTTCTGTAAAG1370.11759151967726707No Hit
TCAACAGGGGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAAC1360.11673318741684906No Hit
TACATGGGATCATTAAAAGACTGCACAAAACCACACCTTGCACAAAGGCCAT1360.11673318741684906No Hit
ATAACAGGGCATAGGTTATTGCATCAACCTCTTGAGCTCAAGCGATCCTCCT1360.11673318741684906No Hit
GATTCTGGGACCACTTGTATTGCAGATGTTTTTTTCACCTTGTAGTGTATAT1360.11673318741684906No Hit
GAAACAGGGAACTAGAGATTTATTGTACAGCATGGTGACTACAGATAAAAAG1350.11587485515643105No Hit
GGTCATGGGATAAGACATGCCTATGAAATGGACACAAAAATTTGGAACACAA1350.11587485515643105No Hit
TAAGCCGGGATATTTATTCTACTGTAAATGACAAAAGAAAAAGAAAAATTGA1340.11501652289601305No Hit
GAGCAGGGTGCCCTCATAGTGTAATGGCACCAGCAGATCTGCCTGTGCACAG1340.11501652289601305No Hit
CGTGAAGGGAGCAACCTTTCTAAAAAAGCAAACCTTTTTCCTGGGAGGAAAA1340.11501652289601305No Hit
ACATGAGGGCAGAATACTCCATCCACGGCCTCTTCTGTCTGATGTTTCTGTG1330.11415819063559504No Hit
ATTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1330.11415819063559504No Hit
CTCCCTGGGTGGCTATTCCTAGAAACACACAACCAACCAAGACTGAATCATA1320.11329985837517703No Hit
TGTGCATGGGTATTAGAGTTCATGGAACATAGAACAACCATGAATGAGTGGC1320.11329985837517703No Hit
GGGTCTGCCCGTTGGGGGTAACAATGATGTGATCATTTCAATTGATATTGAA1310.11244152611475902No Hit
TGACAGCGGGACTCGCGGTCACAGTACTGGAGAGAGACACACAGATCTAAGT1310.11244152611475902No Hit
TAGTAGGGGTGGGGCATCTGGTCATCCATCTTTCTGTCTCCTTCCTGCTGAG1290.110724861593923No Hit
CTAGCCAAGGGGATGCGCTCTGCCCTGGCAGCGGCCCTGTGCAGATCCCTGA1290.110724861593923No Hit
TCTGTTGGGACTGGAGGAAATGAATGCAGTTATTTGCTGCCAGAGGCCAGAG1280.109866529333505No Hit
GCAAATGGGTTTCTTAGTGAGCTGGATTATCTTGAACCTGTGCTATTAAATG1280.109866529333505No Hit
CCAAACGGGCTATTTAGACTGTATAGACTGATTTAGATTACATACTTTAGAG1280.109866529333505No Hit
AGAGGTGGGATCTAGTCGCGGGGATCTGAAAACGGGTAATACTGGCCTCATA1270.109008197073087No Hit
CGTGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.109008197073087No Hit
CGGGGAAGAAGTAATGAATGATTACAATATGCTTAAAGCTCAAAATGATCGA1270.109008197073087No Hit
AGAAAAGGGCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTTGATTC1270.109008197073087No Hit
TTCAGCGGGGAATCTTGTCTCAATGCTCATAACGGATCAGAAATACAGATTT1270.109008197073087No Hit
GAACAAGGGTAAGAAATGCCAAGATTCTTAAATTTGCCAGCGTTAAAGTAGA1270.109008197073087No Hit
GTATTAGGGTAAAAAGTTAACATTATCCTATTTCCATAGATACCATGGACGG1260.10814986481266899No Hit
AGATTCGGGTCTCAGGAAGGGCATTCTTGCTGCATCAGTCAGCACTGTGACT1260.10814986481266899No Hit
ACCACTGGGCTCCACTGTGCACCTGAGGCAGATCATGCATCTTTGAGTGCAG1260.10814986481266899No Hit
AGTCGCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1260.10814986481266899No Hit
GCGACAGGATACCAGTTATATGAATTGACTTAAGTATCTTGAAAAAGAAACT1250.10729153255225098No Hit
TTCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.10643320029183297No Hit
GTCAAGGGGCTAAAGAAGTAGCTGATTGTTTCAAGAAAACATTTGAAGAATG1240.10643320029183297No Hit
GCATTTGGGATGGTGCAGCTTTTTTTTAATTATGTTTTTAAAATTATACAGT1240.10643320029183297No Hit
TCAAGAGGGGTGTACAATTCAGTATTATTGGATATATTCATAATGTTGTGCA1240.10643320029183297No Hit
GTCATGGGATTACAAAATAATTAAAAATCCAATGGATTTGTCAACCATCAAG1240.10643320029183297No Hit
TTCATGGGTTTCTACTGCTTTGACTTTTATTTCTCAAAAAGACTGTTTTGTT1230.10557486803141497No Hit
CGATTTGGGTAGCTTTTCTCAAGCAACATTGGAACAGGTTTTTGTAGAACTC1230.10557486803141497No Hit
GTATGGGGTGACTACCCCCAGAATGAGCTTTGATGGTCTCTACTGGGCCTTT1230.10557486803141497No Hit
AACTCAGGGCTACCAAAGAGAGGGAGTTGACTGGCTTTTAAAAAGTATTTAA1220.10471653577099696No Hit
TTTTGAGGGGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACTTTT1220.10471653577099696No Hit
TCTAGCGGGTCTGCAAGCGGGGTGTTCCAAAACACATCACATTATTAGGTTG1220.10471653577099696No Hit
GAATCCGGGGCCTCCCTTCTTCCGAGCCTCTCCTCTGGCCGCCGCGCGGGAG1220.10471653577099696No Hit
CTCACGTAGGGAGTGAAGCCAGGCGACGAGAACCTTCGGGAGATGAACAAGA1210.10385820351057895No Hit
CACAAAGGGTTTCGATTATAAATGAATGATCACCTCGAAGTCACTTTAGAAC1210.10385820351057895No Hit
TCATTGGGGGATTTGAATACTAGTAGAAACTGGCCAGGAAAAAGGTACATTT1210.10385820351057895No Hit
GATTTGGGGTATCTTTTCTAATGATCAAAAGAGTGTGACTTCTCATTTGTGA1210.10385820351057895No Hit
TATTCTGGGCTGCAAAGGAATCTGCTCTTTGAGGTTTCTTTCTGCACTAGAG1200.10299987125016094No Hit
CAACTTGGGCAGGATACAGATTCTTTTCAGTCAAACATGTTCATTCACCAAG1200.10299987125016094No Hit
TCTGTCGGGCAGCTACTCAGATCTGCACTCCTGTCTCTTGGGAAGCAGTTTC1200.10299987125016094No Hit
GATTTGGGACAGGGACTAGGACATCGACCACCCTCTGCCCCTCCTGCAGTCA1200.10299987125016094No Hit
ATTAGGGGGGATCTCTGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAG1200.10299987125016094No Hit
GAAATGGGATGCAAAGTTCTGCAGAAGAAGGTAAGATGGAGCTTCATCTTCA1200.10299987125016094No Hit
GTACAAGGGATCTGACAGAGGGGGGACAACATGGCGGCCTTCATGCTGGGCT1200.10299987125016094No Hit
ACAGTAGGGGTCCTGGACGTGGGAACGGGCAGGGATTTCATGACAGGGACCC1200.10299987125016094No Hit
CTCTTGCGGGGAATGTTTGTGAACTACTGCTCAATAAATATCCAATAAATGT1200.10299987125016094No Hit
GTATCAGGGGCTCTTCCGATCTCGGAGTGGGGGGAACACTTCTCAACACAAG1190.10214153898974293No Hit
TAATCTGGGATAGCTGTTTTGTATTATAGTGTATATGATGTTTGTGAAAATG1180.10128320672932493No Hit
TTTGCGGGGAGCCTGCATCTTTTGTATAGTAAGGAATGATCTAGTTAGAGCT1180.10128320672932493No Hit
GTATCTGGGTTAGAAAAATATTAAAATTGAGCCTGATGTTGTCTAGGTAAGA1180.10128320672932493No Hit
TTAAGGGGGTGACATATCTAAACATAAGAACTCAAACCATCAGACTTTTAGA1180.10128320672932493No Hit
GTGCTGGGGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAG1180.10128320672932493No Hit
TTCAGTGGGGTTTTTTTAAATTGGTAAAATACTGTACATTCTTTTTATTGTA1180.10128320672932493No Hit
GCTACAGGGACGCCCATTTTCCTACCCACAGCAGCAAATGACAACGTGTCTG1170.10042487446890692No Hit
TGACCTGGGACATGAGCGAACTCATGCAGGGGTCAACCCTTATAAATGCAGT1170.10042487446890692No Hit
AGCTGTGGGGAGTTTGGAGTTTGTGAAGTTCGTGACCTCAAACCCAATGGGG1170.10042487446890692No Hit
TACAGTCGGGAAATTAATGTTTAAAGGTAGTTTTGTTCTTACAGAAAGTGTT1170.10042487446890692No Hit
TCTGTAGGGGTGTGTCTGTTTTAACAATGATGAATGGTGGGGTGAAGGAAAC1170.10042487446890692No Hit
CATCAAGGGCTCTGCATGCCCATGAATGACTGCAAAAATGCCACCAATATTG1170.10042487446890692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)