FastQCFastQC Report
Sat 31 Dec 2022
SRR5844172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101984
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5760.5647944775651083TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5580.5471446501411986No Hit
TTGCTGGGATTACAGGCGTGAGCCACTGCGCCTGGCCCGAATCTTTTCTTTA1890.18532318795105113No Hit
AAAACAGGGATGTGAGAACTAAATTATAAAGATTGAAGCATATAAGAAAAAA1870.18336209601506118No Hit
AGTTTTGGGAAGGCTGTTTTTTAAAATGTTTATTTGAAAGACAATTTTCTTT1860.1823815500470662No Hit
AACCTAGGGAACCTTGAGAATTACATTTTCTAAAAAGGAAGAACCACCTAAT1760.1725760903671164No Hit
GCACAAGGGGGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCAGCCAT1740.17061499843112646No Hit
TAACACGGGTAGTTGAGTCAAGTCCCGAAAGGTGACGTGGTTGGCCTGTAGT1690.16571226859115157No Hit
TAAGCTGGGAATTATGCTGAATGAAAGAAACCAGACAAAAAAGAATACATAC1670.1637511766551616No Hit
AGCATGGGGTCAACACTCCACTTTCAGTATTAGACAGATAATCTAGGCAAAA1630.15982899278318166No Hit
GTCCCAGGGGGGATGCAAAAAGCACAGGCAATCAAACAAAAATAAACAAGTG1600.15688735487919672No Hit
TCCAAAGGGAGACTCTTTAAGCATAAGGATGAAAATTTGGTTTTATCCATTT1600.15688735487919672No Hit
CATTGCGGGAAGGCCCAGTGGGCTGTCCCTGACGACCTCAGGCTTCACTCCA1590.15590680891120176No Hit
AACAAGGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT1580.15492626294320677No Hit
GTACTGGGGAGTAGTTGGGTATATGAAACAGGACGTTTGTGTGAACCAGAGG1560.15296517100721682No Hit
CTTACAGGGCAAAACGTGCAGACCACTAACGAATGACAAGAGTGAAGCTGTA1560.15296517100721682No Hit
AATAGTGGGACAACCAAATACAACTGGGCCAATATAAACATGTTTGAATTGT1550.15198462503922183No Hit
CACTTAGGGGGATGCAAATACTATAAAAAGAGACAAATATGTGCCTCATAAT1520.14904298713523692No Hit
ATGAAAGGGGAATGAGGAGAAAAGAACAATCAAAAAGACAGTTGTTATAAAG1520.14904298713523692No Hit
CCACTAGGGACTACACAGGAATGCTCCAAAATGGGAAGAAGTTTGATTCATC1520.14904298713523692No Hit
ATGAAAGGGAGGGACTGATTGGTCCCATTTGCCCTCAGGTCAGTTGTTTAAT1470.144140257295262No Hit
TACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1440.14119861939127706No Hit
TGTTGTGGGCTGGGTCAGATTAGTAATTCTAATTAACTTAAGATACCTATTT1440.14119861939127706No Hit
TTGCTTGGGGATGGTGAAAAGGACAATTTTGGTACATCACAGACTCCATTAG1430.14021807342328207No Hit
ACGAGACGGGATACTTTTCCATGGAGGATTAAGTAAACTGAAACCTAAGACA1430.14021807342328207No Hit
ACACCCGGGATGTGATTTCAGTATATACAACTCCACCAGACCCCTCCAACCC1420.1392375274552871No Hit
CGAGAAGGGATGTTGAATGAATGTATGAATTCTAAATTGTAAGATTTGAAAT1410.13825698148729212No Hit
TACCCAGGGACTCATAAAAATAGTTGCTTCAATACAGCTCAAACTCAGCCAG1410.13825698148729212No Hit
CAGCTAGGGAGTTTTCTGCTGCTTGAATGACTTTTTTCAACATTTCTTGTAG1410.13825698148729212No Hit
TCCGCTGGGCAAATAAAATTCCAATTTGTTTCCAAGTAATTAAAATCAATAC1400.13727643551929714No Hit
TTGCTGGGATTGCAGGCATGAGCCACAGCGCCTGGTCCATAATACTTTTTAA1400.13727643551929714No Hit
GCAACCGGGCGATCTTACGTCGGGACATACGCTGATCTAGGAATGAAATCTT1390.13629588955130217No Hit
ACAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1390.13629588955130217No Hit
AACTTTGGGGCATGGTTTAACTTCCTCATCAGAACTCTGCCCTTCCTTCTGT1380.13531534358330718No Hit
TGCAATCGGGGTTCCAGAAGAAGGCATTGCTATCATAGAAGATGACAGCTCC1380.13531534358330718No Hit
TCTTTGCGGGAGAATTTGAGTGATCTATGCTGAATAAACAGTGGAATGTGAC1370.1343347976153122No Hit
GTATGGGGGGTTCATCTTCCACCTACAAATTTAATACTTATTCCACCTTAAC1370.1343347976153122No Hit
CTGGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1360.13335425164731723No Hit
GCTGCGGGATTTTTAAAAGATGTTAAATGTTCTTTTCATTGTGGTGCTTCAG1360.13335425164731723No Hit
TGGCCGGGAAGGCCAATGTAATTAATAAACAGGAACATGATATTATTAAACA1350.13237370567932225No Hit
TTTGCTGGGAGGCAGCGCTCCAGTCGGGAATGGCCAGGATGGCGCCCTCTTG1350.13237370567932225No Hit
TGCACTTGGGGGTGTGCTCCTGAATCAGTTCATGGTAGATTATGCTGAACAA1350.13237370567932225No Hit
CTCTCTGGGGTAGAAAAGTTACAGGATAATATGAAGGTCCTGTATATCCCAC1340.13139315971132726No Hit
GCCTATGGGGGAGAAAGACCTTATAAGTGCAGTGAATGTGGAAAATTCTATA1340.13139315971132726No Hit
TGGCGGGCGATCTTGAAGTGGGACTTGTCACCTCGAATTGAGCATGCACTTG1330.1304126137433323No Hit
CGTACAGGGTGCTTTCAAGGGACAAGTATGTTTGAGTTTTGGCTTATAATTT1330.1304126137433323No Hit
TCATAAGGGATTCCTAAGCACGTTGAGGTCTGAGAAGCATTTCCCAGACTCC1320.1294320677753373No Hit
TTGCTGGGGAGTCAAAGCACAGCTGGTTGAAGAATGGGCAAACTCTGTGAAG1320.1294320677753373No Hit
GGTCGCTGGGGTTTGTCTTGAAAGTATTTTCATTTCATTTGTTTCTAAAATA1320.1294320677753373No Hit
CTGAATGGGCTCCAGAGTTTCTCTGTTGGAAAGCAGTATCAAGAAGCCTTTA1320.1294320677753373No Hit
ACTTGCGGGTAAACATTATAGTTCACAAAAAGAATAACAATGGAATTAGAGC1320.1294320677753373No Hit
TTTCCAGGGGGGATTATCCAAGGGAAGCTCAGGACATGAGGCACAGGCAGCA1320.1294320677753373No Hit
CAAGTTGGGCAGAGTTATTCACATCATAAATGGGAATTATAAAATGATAGGA1320.1294320677753373No Hit
TCAGAGGGGGATTTTCTTCTAGTATGCAGTTAAGCTACTTGGAATCTCTTGT1320.1294320677753373No Hit
ATGTTTGGGGTATCATATAAAATGATACTGCCTGGTATGCAAATCAGCCAGT1310.12845152180734232No Hit
TAGAGGGGGATTGAAAAACACTATGAATAATTCAAAAACAGAATTCTAAAAA1310.12845152180734232No Hit
TGATCAGGGTAGTAGAATGGACACAGCTGAGGAAATAAAAAACTCTTGAGCA1310.12845152180734232No Hit
TACAACGGGGACTAAAGGATTGAACATACTGAATTCTCAGAGATAGGAAACC1300.12747097583934733No Hit
GCGGACGGGGGAAAGAAAATGATGTCTGTTGTTATAGTTCATTGTTTTGCCT1300.12747097583934733No Hit
TTCAGGGGGGGAAGGATAAGGATTGGATTGTTACTTATTGCTAGGAAGAGAG1300.12747097583934733No Hit
TAGACAGGGGAGTCTGCTTCAGTGAATTAATATATTTTAAAATAACATTTTT1290.12649042987135237No Hit
CATGTCGGGGTAGCATATGTAATTAGTGATACATGTACTTCTCTCTTTGCCT1290.12649042987135237No Hit
CATAGTGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC1280.12550988390335738No Hit
GATATAAGGGGATATTTTTAATATTGCAATACTTTTGCCTTTTCCAGAATGT1270.1245293379353624No Hit
GTGAAAGGGTCGCGCTATGGGACAGAGCCCCCGATCCGCCAGCACCACCTGA1270.1245293379353624No Hit
CAGCCAGGAAACAACAAAGTCTTCTTCAGTCTGACAATATGCACAGACTCTC1260.12354879196736741No Hit
ATTCCTGGGCGAATTACAGTTCCTATAATCTTAGGAAATTATTTTGTGATCA1260.12354879196736741No Hit
ACTTGCGGGAGAATGTTCAAGATTTGCTGCTCTTGGATGTCACTCCTCTTTC1260.12354879196736741No Hit
GGGCAAGGGGGGATATGAGAGATATTAAAGATTGGATTGTCTAGCCATTCAT1260.12354879196736741No Hit
CTTTTTGGGGAAAAAGGAAACGAAAAAGTTCTACATCTGGCTCAGATTTTGA1260.12354879196736741No Hit
CGTAAAGGGACAGTAATTTTATGATTGGCACTTAACAAAAGAAATTAGTATA1260.12354879196736741No Hit
ACTACTGGGATTTAAGAAAACTGAAATTATGTTAAGCACTGTCTCAGACCAC1250.12256824599937247No Hit
ACTGTTGGGATGGTTCAAATAGGACTACTAGAGTTCATTGAACACTAAAACT1250.12256824599937247No Hit
TCCGCAGGAGCATTAGAGCTTCAAGGGCAACTGGATGCATTAAATACATTAG1240.12158770003137748No Hit
CTGATTGGGCTCTCAGTCCTTCCTACAAACTTAGAAAGTCTAATATCTTAAT1240.12158770003137748No Hit
TAAGCTGGGCGGTAGAGATGCAACATGGTACAGCCACTGTGGAAGACAGGTC1230.1206071540633825No Hit
CGAAGAGGGAAATGATCTGAATAGACATTTCTCAAAAGAGACATACATTTAT1220.11962660809538753No Hit
TAGCGGGGGAATGATCTAGAATCTCAAATAAGGCAGGACATATCCAATGCTG1220.11962660809538753No Hit
ATGCATGGGATGATGGTTAAGACAAAAACTGAGTTATCCATCTCCTTTTACT1220.11962660809538753No Hit
TGTTCAGGGCAGCTGACAGCAGAAAAAAGGGTTGCCACCCCAGTTGATTGGA1220.11962660809538753No Hit
ACTCCCGGGCTAGACATCTAGTGTTTGCTGTCATTAGTGACCAAGAAAAAGT1210.11864606212739254No Hit
AATGAGGGGAATTTAAAGAGTTACATAATCCATTCATATTCTTTTAATTATT1200.11766551615939756No Hit
AGCTGTGGGGGATACAACAGTTAATGGTACAGTGTCAGCCGTGAGGCTCTTA1200.11766551615939756No Hit
ATAGCTGGGCAATCAGACCACCACCCTTCAGGGTATTACGCGTGGATAAGTC1200.11766551615939756No Hit
TTTCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.11766551615939756No Hit
AAAAGCGGGGGCTGTGCCCCCTATGCCCTGTGGCATTTCTGCGTCTTACTGG1190.11668497019140257No Hit
ACTTGTGGGTGTGTTGAGCACTCAGAGTGTAGTCAACAGTAAGTTCTTTTAA1190.11668497019140257No Hit
CATGGTCGGGATGAAAGACTTTCCTCCTTTACAAGAAAGAAATGGGGTGCTG1190.11668497019140257No Hit
CGAACCGGGATTTCAAGACCAAAGCAAAGTCTTACTACTACTGTGGAACCAT1190.11668497019140257No Hit
TGCAGTGGGGTTCCCCTTCCTGTGTCCAGGTGTTCTCATTGTTCAATCCCCA1180.1157044242234076No Hit
GCCAAGGGGTCATATGTGAAGATGTCAATAAGCTTGCATTAAGCCACCTGCT1180.1157044242234076No Hit
GTGACAGGGAATTCAAATAGATTAAAGGAATCATTCATCATTATCAAGTGGG1180.1157044242234076No Hit
TCACTCGGGCACGTCACTGCACTCTAGCCTGGGCAACAGAGTAAGACAAAAA1180.1157044242234076No Hit
TACCTTGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG1180.1157044242234076No Hit
AAGGAAATAAAAATTCTGTCTCTCTGGAATTAGGAAGAGCATGCAGAACGTT1170.11472387825541262No Hit
CAACAAGGGATTAAAAAGTAACTTTAGCAATTTTACTAAATAACAAGGTTAA1170.11472387825541262No Hit
TTAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.11472387825541262No Hit
ATTTAAGGGAATCAATCAATCAGTAGACGTCAGAGATAAGCAACTTTTTTTT1160.11374333228741763No Hit
AGACATGGGAATGTATAGTATAAAAAGTTACCCGTGGTATTAAGTGACAAAA1160.11374333228741763No Hit
CATAGTGGGGTGCAATAATTGAGAAACAAAGCCTTAATTGTAAATTAAAAAG1160.11374333228741763No Hit
AGGTATGGGGTAATAGCATTAAATGCTTAATTAGAAAAGAGGAATGGATACC1160.11374333228741763No Hit
GAGTCAGGGCGGGCTATTGTGTTAGATCCCATGGTTGGGTAAGAAGCTCTAC1160.11374333228741763No Hit
CAAATGGGGCTGTTGGATAAGGGATAAGGTGAGTGTTTAGAGCTTTAGGTTA1140.11178224035142767No Hit
ATACAAGGGAAGGTCTCAGTCTGAGGATTTTTGCTTGATATAGGACCTGTAT1140.11178224035142767No Hit
TTTAGAGGGCATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGAGA1140.11178224035142767No Hit
TGTCGTGGGAGAGGAGGACATTTGGGCTCACCTGTCAAGGTGGCCCTGGGAC1130.1108016943834327No Hit
TGCTAAGGGGAGATCTCTTCGGGTTAGTGCAAGGTTGCTGTGATGTTAACCT1130.1108016943834327No Hit
AGACTGGGGGGCAGAAATAAAAATTTCTTTGAAATAAATAAAAACGGAGGTA1130.1108016943834327No Hit
CATGCTTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATCACTTGAG1130.1108016943834327No Hit
GTAGTGGGGATCTCAGTGCCCCAGATAGCAATGCAGTCAAGCTCCCCTGGGA1130.1108016943834327No Hit
TTACTAGGGAGGCTTCCTGAGTCTTGCTGTGTTCTTTTTTTGTGAATAGTAA1120.10982114841543772No Hit
TTCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10982114841543772No Hit
GTTTCTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1120.10982114841543772No Hit
TTACATGGGGATCTCATCACGGGAAGCCATTAATTGTTGGTGTGAATTTAAA1120.10982114841543772No Hit
CTAATGGGGGCAAACACTTTGCTCTGTGGTGTCACAGCTTTGTGACAATAAG1120.10982114841543772No Hit
CTCACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1120.10982114841543772No Hit
AGACCTGGGATGTCCTTTGTTCCCGGTCTGCTCTTGTGTTTCGTTCTCCTCC1110.10884060244744273No Hit
TGCTTAGGGAGCTCTGGGTTGTGCCATTCAAGAAAAGGTTTCATGGGTATGT1100.10786005647944776No Hit
TGGCCCGGCTAATTTTTTGTATTTTTAGCACAGACGGGGTTTCACTGTGTTA1100.10786005647944776No Hit
GTCAAAGGGAAGCTTTTGGGTAATATAAAAATAGCTCTGGAAAAATTGCAAG1100.10786005647944776No Hit
AGTCATGGGATTAAGCAGTATTAAGAAAATGTAGCAATATTGAAATGATTTT1100.10786005647944776No Hit
AGCCATGGGGGGATAGGCTGCTTCCTAGGGGCTGCTAGCAGGATCGTTAACT1090.10687951051145278No Hit
TACACAGGGAACTTAAGGAATAGAGTATCAGAAATTAACCATTAAATTAATA1090.10687951051145278No Hit
TACACTGGGATAACCCTGGCACCTAATATAGTGTGGGAATCACAGCAGCAGC1090.10687951051145278No Hit
TTTAATGGGAAATATAAAAGATGGTAAGAACTGATTTCCAGAAGGACTTCAA1080.10589896454345779No Hit
GGCCAGGGGTTTTATTTTTAAGAGTATACTTTTTAAAGGATAAATATCGAGG1080.10589896454345779No Hit
TGCTAAGGGCAGAAACATTTTGTGGCATACAGCATTCCTGGGAAGTGCTGTA1080.10589896454345779No Hit
GTGGCTGGGAGAAACATAACAAACAACTTGGCTTACTTTCTTCTGCGAAGCA1080.10589896454345779No Hit
ATTTTGGGGGAGCACATCTCAGCTGAAAACATGTCTTTAGAAACTCTGAGAA1080.10589896454345779No Hit
TGCAATGGGATTCCATCGAGATTTTAAGCAGCACCATCAAGCCTTACAATGC1070.10491841857546282No Hit
CTTGTGGGGCACAGTCTGGTGGTGACATGGCAGGGAATTTTACCTAAGATAA1070.10491841857546282No Hit
TGAATGGGGGTCAGTGGTTCCAACTCTTTGTCTTACATTAAAATGAAGGGGC1060.10393787260746784No Hit
TGACGAGGGATTTTGTAGGAAATAAAAGAAAGAAAGTTCTTTCCTATATTGA1060.10393787260746784No Hit
CGTAGAGGGAAGAATATTCTCCATTTTGTAGAACATGCTCTGAAGTTACACA1060.10393787260746784No Hit
ATAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1060.10393787260746784No Hit
CCTTCCGGGTGATACTATAGGTACTATAGGTTTGAGTGTTAGGAGTTTTCTT1050.10295732663947285No Hit
TCCAGTGGGTCTAAAGGGAGGCCTTCCCCAAGAACCCAGTTTTTATTAGAGC1050.10295732663947285No Hit
TCGCTCGGGTGTTAAATTTCTTGTACAAAATTGTTTATTTGCCTTTTCTTTG1050.10295732663947285No Hit
CGACATGGGGTTCATCAATGGGCAAATGGATAAAGAAACTGTGATATAGAGA1040.10197678067147788No Hit
AGCAAGGGGCAAGCCATGGAGACAGGTAGCATTTGTAAGATGCTGCACAGGA1040.10197678067147788No Hit
TATTAGGGGTCTTGTTTTTAGTCTCGCTTTTCGGTTGCCGTTGTCTTTTTTC1040.10197678067147788No Hit
ACAAGCGGGCAACATTTTATGGTCCAACTCAAAGTAAACAAGTATGGAGATA1040.10197678067147788No Hit
TCAATCGGGTATGAGAAATAAATTCGTACTTTTAAATTACCCAGTCTCAGGT1030.10099623470348289No Hit
CTACGTGGGGAGTCCAGGTTTTTGCTTTTGGAAGGTCTCTGATAATTTAGGG1030.10099623470348289No Hit
GTGGCAGGGGCTGGCTATTCTATCCATGAAGATGACCCTACAGTAAAATACC1030.10099623470348289No Hit
CTAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.10099623470348289No Hit
GGTATAGGATACTATTGTGTGACTGTGGAATAACATCTCTAGTGATTTCTTT1030.10099623470348289No Hit
CGATTCGGGTGAGGTGAGACTTCCTTTGTCTTTCCCTGACCCCACTATCCTG1030.10099623470348289No Hit
GTCATTGGGCTTTGGGTCAGAAAAAATAAAACAGTGCACATAATCTAATAGC1030.10099623470348289No Hit
TATCCTGGGGACAAATCTCCCATAAGGAAGAATTACAAATAGTTCTTGTAGT1030.10099623470348289No Hit
CAGCACGGGCAGGAGTCCAAGCACAAAGCGGTCTTTTACATTCCAACCTGTT1020.10001568873548791No Hit
CATTCTGGGGTAGAATCATGGAACACAAGATCAGGAAGGGCTCTTAGAGACC1020.10001568873548791No Hit
GTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAAAAATGAAACTAAATGAA1020.10001568873548791No Hit
TGAATAGGGATTGGTTAATCATGCACCTTTGTGCCATGTTTAACTCTTTTAT1020.10001568873548791No Hit
CCCCAGGGAGGCCCGAAGCTGTTCCCATGCTTGAGCTTCCATGGGGAGGATG1020.10001568873548791No Hit
TCACTCGGGATCGTTGGCTGTGGATGTGGAAAACCTTTCCTAGCTTAGAGCA1020.10001568873548791No Hit
TAACTAGGGAAGCTTCAGTTGTGTGATTCCAGATGATGTATGTGAGGCCTAA1020.10001568873548791No Hit
GCAAGACGGGCAGTGCTTTTATTTTACAGAGGATATATGCTAATGCCTAAAA1020.10001568873548791No Hit
AACGGAGGGGAGCGTCAGGGGAGCTGGGTCAGGCGGTCGCCGGGACACCCCG1020.10001568873548791No Hit
TATTCTGGGGAGACCAAGGGCTAAAGCTGGGAGACTGAAAAAATGCAGACCG1020.10001568873548791No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)