FastQCFastQC Report
Sat 31 Dec 2022
SRR5844171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102057
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT6640.6506168121735892TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5750.5634106430720088No Hit
AACAAGGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT1980.1940092301361004No Hit
AGTTTTGGGAAGGCTGTTTTTTAAAATGTTTATTTGAAAGACAATTTTCTTT1980.1940092301361004No Hit
CATTGCGGGAAGGCCCAGTGGGCTGTCCCTGACGACCTCAGGCTTCACTCCA1890.18519062876627765No Hit
AGCATGGGGTCAACACTCCACTTTCAGTATTAGACAGATAATCTAGGCAAAA1820.17833171658974886No Hit
AAAACAGGGATGTGAGAACTAAATTATAAAGATTGAAGCATATAAGAAAAAA1760.17245264900986704No Hit
TAAGCTGGGAATTATGCTGAATGAAAGAAACCAGACAAAAAAGAATACATAC1680.16461389223669126No Hit
TACCCAGGGACTCATAAAAATAGTTGCTTCAATACAGCTCAAACTCAGCCAG1660.1626542030433973No Hit
GTCCCAGGGGGGATGCAAAAAGCACAGGCAATCAAACAAAAATAAACAAGTG1630.1597146692534564No Hit
CCACTAGGGACTACACAGGAATGCTCCAAAATGGGAAGAAGTTTGATTCATC1620.1587348246568094No Hit
GCACAAGGGGGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCAGCCAT1600.15677513546351549No Hit
TTTGCTGGGAGGCAGCGCTCCAGTCGGGAATGGCCAGGATGGCGCCCTCTTG1570.15383560167357457No Hit
AACCTAGGGAACCTTGAGAATTACATTTTCTAAAAAGGAAGAACCACCTAAT1560.1528557570769276No Hit
ACGAGACGGGATACTTTTCCATGGAGGATTAAGTAAACTGAAACCTAAGACA1560.1528557570769276No Hit
AATAGTGGGACAACCAAATACAACTGGGCCAATATAAACATGTTTGAATTGT1560.1528557570769276No Hit
CTTACAGGGCAAAACGTGCAGACCACTAACGAATGACAAGAGTGAAGCTGTA1560.1528557570769276No Hit
TTGCTGGGATTGCAGGCATGAGCCACAGCGCCTGGTCCATAATACTTTTTAA1560.1528557570769276No Hit
GTACTGGGGAGTAGTTGGGTATATGAAACAGGACGTTTGTGTGAACCAGAGG1550.15187591248028062No Hit
CACTTAGGGGGATGCAAATACTATAAAAAGAGACAAATATGTGCCTCATAAT1530.1499162232869867No Hit
TTGCTTGGGGATGGTGAAAAGGACAATTTTGGTACATCACAGACTCCATTAG1530.1499162232869867No Hit
ATGAAAGGGAGGGACTGATTGGTCCCATTTGCCCTCAGGTCAGTTGTTTAAT1520.1489363786903397No Hit
TCCAAAGGGAGACTCTTTAAGCATAAGGATGAAAATTTGGTTTTATCCATTT1470.14403715570710485No Hit
CGAGAAGGGATGTTGAATGAATGTATGAATTCTAAATTGTAAGATTTGAAAT1450.14207746651381092No Hit
TGCAATCGGGGTTCCAGAAGAAGGCATTGCTATCATAGAAGATGACAGCTCC1450.14207746651381092No Hit
AGCAAGGGGCAAGCCATGGAGACAGGTAGCATTTGTAAGATGCTGCACAGGA1440.14109762191716393No Hit
TTTCCAGGGGGGATTATCCAAGGGAAGCTCAGGACATGAGGCACAGGCAGCA1440.14109762191716393No Hit
TCCGCAGGAGCATTAGAGCTTCAAGGGCAACTGGATGCATTAAATACATTAG1440.14109762191716393No Hit
CAAGTTGGGCAGAGTTATTCACATCATAAATGGGAATTATAAAATGATAGGA1430.14011777732051695No Hit
TAGAGGGGGATTGAAAAACACTATGAATAATTCAAAAACAGAATTCTAAAAA1420.13913793272387No Hit
TAACACGGGTAGTTGAGTCAAGTCCCGAAAGGTGACGTGGTTGGCCTGTAGT1420.13913793272387No Hit
GTCAAAGGGAAGCTTTTGGGTAATATAAAAATAGCTCTGGAAAAATTGCAAG1410.13815808812722302No Hit
TGTTCAGGGCAGCTGACAGCAGAAAAAAGGGTTGCCACCCCAGTTGATTGGA1390.13619839893392907No Hit
TTACATGGGGATCTCATCACGGGAAGCCATTAATTGTTGGTGTGAATTTAAA1380.1352185543372821No Hit
CGTAAAGGGACAGTAATTTTATGATTGGCACTTAACAAAAGAAATTAGTATA1380.1352185543372821No Hit
TCTTTGCGGGAGAATTTGAGTGATCTATGCTGAATAAACAGTGGAATGTGAC1370.13423870974063515No Hit
AGACTGGGGGGCAGAAATAAAAATTTCTTTGAAATAAATAAAAACGGAGGTA1370.13423870974063515No Hit
GTATGGGGGGTTCATCTTCCACCTACAAATTTAATACTTATTCCACCTTAAC1360.13325886514398816No Hit
GGTCGCTGGGGTTTGTCTTGAAAGTATTTTCATTTCATTTGTTTCTAAAATA1350.1322790205473412No Hit
TTGCTGGGATTACAGGCGTGAGCCACTGCGCCTGGCCCGAATCTTTTCTTTA1350.1322790205473412No Hit
TTTCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1350.1322790205473412No Hit
TTTAGAGGGCATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGAGA1340.13129917595069424No Hit
AACTTTGGGGCATGGTTTAACTTCCTCATCAGAACTCTGCCCTTCCTTCTGT1330.13031933135404725No Hit
AGACCTGGGATGTCCTTTGTTCCCGGTCTGCTCTTGTGTTTCGTTCTCCTCC1330.13031933135404725No Hit
TCAATCGGGTATGAGAAATAAATTCGTACTTTTAAATTACCCAGTCTCAGGT1330.13031933135404725No Hit
CATGTCGGGGTAGCATATGTAATTAGTGATACATGTACTTCTCTCTTTGCCT1330.13031933135404725No Hit
GTGCTCGGGATGTACACACAGCTTTATCATCGGGTAAGTGAAAATGAAAATG1320.12933948675740026No Hit
CGTACAGGGTGCTTTCAAGGGACAAGTATGTTTGAGTTTTGGCTTATAATTT1320.12933948675740026No Hit
TTTTTAGGGACTTGGGGGAGGACATGTTGCAGAAGACCAGATCATTTTTATA1320.12933948675740026No Hit
TTCAGGGGGGGAAGGATAAGGATTGGATTGTTACTTATTGCTAGGAAGAGAG1320.12933948675740026No Hit
AAGGAAATAAAAATTCTGTCTCTCTGGAATTAGGAAGAGCATGCAGAACGTT1310.1283596421607533No Hit
TGGCCCGGCTAATTTTTTGTATTTTTAGCACAGACGGGGTTTCACTGTGTTA1310.1283596421607533No Hit
ATAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1310.1283596421607533No Hit
AACCTTGGGAAGTGACAGGGTGATAGAAGTGTGGTAAGTGATTAGGATAATA1310.1283596421607533No Hit
TGATCAGGGTAGTAGAATGGACACAGCTGAGGAAATAAAAAACTCTTGAGCA1310.1283596421607533No Hit
TGGCCGGGAAGGCCAATGTAATTAATAAACAGGAACATGATATTATTAAACA1300.12737979756410633No Hit
TGCAGTGGGGTTCCCCTTCCTGTGTCCAGGTGTTCTCATTGTTCAATCCCCA1300.12737979756410633No Hit
TGCTAAGGGCAGAAACATTTTGTGGCATACAGCATTCCTGGGAAGTGCTGTA1300.12737979756410633No Hit
TAGACAGGGGAGTCTGCTTCAGTGAATTAATATATTTTAAAATAACATTTTT1300.12737979756410633No Hit
ATGTTTGGGGTATCATATAAAATGATACTGCCTGGTATGCAAATCAGCCAGT1290.12639995296745937No Hit
ACTTGCGGGAGAATGTTCAAGATTTGCTGCTCTTGGATGTCACTCCTCTTTC1290.12639995296745937No Hit
GCAACCGGGCGATCTTACGTCGGGACATACGCTGATCTAGGAATGAAATCTT1290.12639995296745937No Hit
ATGAAAGGGGAATGAGGAGAAAAGAACAATCAAAAAGACAGTTGTTATAAAG1290.12639995296745937No Hit
CTCACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1290.12639995296745937No Hit
TCATAAGGGATTCCTAAGCACGTTGAGGTCTGAGAAGCATTTCCCAGACTCC1270.12444026377416541No Hit
ACACCCGGGATGTGATTTCAGTATATACAACTCCACCAGACCCCTCCAACCC1270.12444026377416541No Hit
ACTGTTGGGATGGTTCAAATAGGACTACTAGAGTTCATTGAACACTAAAACT1270.12444026377416541No Hit
TGCACTTGGGGGTGTGCTCCTGAATCAGTTCATGGTAGATTATGCTGAACAA1270.12444026377416541No Hit
TGGCGGGCGATCTTGAAGTGGGACTTGTCACCTCGAATTGAGCATGCACTTG1260.12346041917751846No Hit
CTGATTGGGCTCTCAGTCCTTCCTACAAACTTAGAAAGTCTAATATCTTAAT1260.12346041917751846No Hit
GTGAAAGGGTCGCGCTATGGGACAGAGCCCCCGATCCGCCAGCACCACCTGA1250.12248057458087147No Hit
GCCTATGGGGGAGAAAGACCTTATAAGTGCAGTGAATGTGGAAAATTCTATA1250.12248057458087147No Hit
CATAGTGGGAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGCCTGGC1250.12248057458087147No Hit
TTAACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.12248057458087147No Hit
TTGTCTGGGACCCCATTATCCTAGAAGGTCTTGATTTGGTGGAAGAAGATGA1240.1215007299842245No Hit
CTGAATGGGCTCCAGAGTTTCTCTGTTGGAAAGCAGTATCAAGAAGCCTTTA1240.1215007299842245No Hit
CAGCTAGGGAGTTTTCTGCTGCTTGAATGACTTTTTTCAACATTTCTTGTAG1240.1215007299842245No Hit
AATGAGGGGAATTTAAAGAGTTACATAATCCATTCATATTCTTTTAATTATT1230.12052088538757753No Hit
ACTCCCGGGCTAGACATCTAGTGTTTGCTGTCATTAGTGACCAAGAAAAAGT1230.12052088538757753No Hit
ACTTGTGGGTGTGTTGAGCACTCAGAGTGTAGTCAACAGTAAGTTCTTTTAA1230.12052088538757753No Hit
CTCTCTGGGGTAGAAAAGTTACAGGATAATATGAAGGTCCTGTATATCCCAC1220.11954104079093056No Hit
TCCGCTGGGCAAATAAAATTCCAATTTGTTTCCAAGTAATTAAAATCAATAC1220.11954104079093056No Hit
TGACGAGGGATTTTGTAGGAAATAAAAGAAAGAAAGTTCTTTCCTATATTGA1220.11954104079093056No Hit
TGCTAAGGGGAGATCTCTTCGGGTTAGTGCAAGGTTGCTGTGATGTTAACCT1210.11856119619428357No Hit
GTGACAGGGAATTCAAATAGATTAAAGGAATCATTCATCATTATCAAGTGGG1210.11856119619428357No Hit
TCAGAGGGGGATTTTCTTCTAGTATGCAGTTAAGCTACTTGGAATCTCTTGT1190.11660150700098963No Hit
CTAATGGGGGCAAACACTTTGCTCTGTGGTGTCACAGCTTTGTGACAATAAG1180.11562166240434268No Hit
ATAGCTGGGCAATCAGACCACCACCCTTCAGGGTATTACGCGTGGATAAGTC1180.11562166240434268No Hit
TTGGATGGGATTTCATCGTGGGAATTTGGGAAAATCATCATGATAATGAGCT1170.1146418178076957No Hit
CGAAGAGGGAAATGATCTGAATAGACATTTCTCAAAAGAGACATACATTTAT1170.1146418178076957No Hit
TGAATAGGGATTGGTTAATCATGCACCTTTGTGCCATGTTTAACTCTTTTAT1170.1146418178076957No Hit
GTTTCTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1170.1146418178076957No Hit
CATGGTCGGGATGAAAGACTTTCCTCCTTTACAAGAAAGAAATGGGGTGCTG1170.1146418178076957No Hit
TTGCTGGGGAGTCAAAGCACAGCTGGTTGAAGAATGGGCAAACTCTGTGAAG1160.11366197321104872No Hit
AGCTGTGGGGGATACAACAGTTAATGGTACAGTGTCAGCCGTGAGGCTCTTA1160.11366197321104872No Hit
TACACAGGGAACTTAAGGAATAGAGTATCAGAAATTAACCATTAAATTAATA1160.11366197321104872No Hit
ACTTGCGGGTAAACATTATAGTTCACAAAAAGAATAACAATGGAATTAGAGC1160.11366197321104872No Hit
CATAGTGGGGTGCAATAATTGAGAAACAAAGCCTTAATTGTAAATTAAAAAG1150.11268212861440176No Hit
CCTTCCGGGTGATACTATAGGTACTATAGGTTTGAGTGTTAGGAGTTTTCTT1150.11268212861440176No Hit
CAGCCAGGAAACAACAAAGTCTTCTTCAGTCTGACAATATGCACAGACTCTC1140.11170228401775478No Hit
ATTCCTGGGCGAATTACAGTTCCTATAATCTTAGGAAATTATTTTGTGATCA1140.11170228401775478No Hit
ATTTAAGGGAATCAATCAATCAGTAGACGTCAGAGATAAGCAACTTTTTTTT1140.11170228401775478No Hit
TCCAGTGGGTCTAAAGGGAGGCCTTCCCCAAGAACCCAGTTTTTATTAGAGC1140.11170228401775478No Hit
TACTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.11072243942110781No Hit
GGTAATGGGAGAGGGAGTAAAACTATTTGCAAGCCAGGCAAATAACAATTCT1130.11072243942110781No Hit
TGTTGTGGGCTGGGTCAGATTAGTAATTCTAATTAACTTAAGATACCTATTT1130.11072243942110781No Hit
ATCAAAGGGATTTGGAGCAGTTATTTTATTTTTGTTAGTACTTACTTGTAAA1120.10974259482446085No Hit
ATGCATGGGATGATGGTTAAGACAAAAACTGAGTTATCCATCTCCTTTTACT1120.10974259482446085No Hit
AGGTATGGGGTAATAGCATTAAATGCTTAATTAGAAAAGAGGAATGGATACC1120.10974259482446085No Hit
GGCCAGGGGTTTTATTTTTAAGAGTATACTTTTTAAAGGATAAATATCGAGG1110.10876275022781387No Hit
TACAACGGGGACTAAAGGATTGAACATACTGAATTCTCAGAGATAGGAAACC1110.10876275022781387No Hit
TTACTAGGGAGGCTTCCTGAGTCTTGCTGTGTTCTTTTTTTGTGAATAGTAA1110.10876275022781387No Hit
AACCTGGGGAACATTGAATATTATCAATATTCTCAGAGCTCTTAACAAGTAA1110.10876275022781387No Hit
CACCGTTTCTGGTTGTCTACACCTGCCTTTTTCACCGTTGCAGGTGACGTTG1110.10876275022781387No Hit
CCCCAGGGAGGCCCGAAGCTGTTCCCATGCTTGAGCTTCCATGGGGAGGATG1110.10876275022781387No Hit
CTGTCGGGGCCTTGGACAAGATTCGCTATGAGAGCCTGACAGACCCTTCGAA1110.10876275022781387No Hit
ACGTAGGGGGCGGAGGACCGTGGGCAGCCAGGGTCGGTGAAGGATCCCAAGA1110.10876275022781387No Hit
GCTCAGGGAGGCGCCGAGGAGGGGAGCGGGAAGCGCAGGTGATGCTGTGGAG1100.10778290563116691No Hit
TAAGGACGGGGGTGTATGTCAAAGAATAAATTAGTAAAATTAGTAAATGAAA1100.10778290563116691No Hit
ACTACTGGGATTTAAGAAAACTGAAATTATGTTAAGCACTGTCTCAGACCAC1100.10778290563116691No Hit
CAAACTGGGGATGACACAAAACTATGAGAGTGACAAAATGGTGACAGGTAGC1100.10778290563116691No Hit
CATGCTTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGAGGATCACTTGAG1100.10778290563116691No Hit
ACGTGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1100.10778290563116691No Hit
AAGTTAGGGGCAGTGTTAAGAGAACTTAAAAACCATGCACATTTTCAGTTTC1090.10680306103451993No Hit
TTGTCCGGGGGATTTTTAAAAATAAAAAAATAAAAATAGAAAAGTTGCACAA1090.10680306103451993No Hit
GGGCAAGGGGGGATATGAGAGATATTAAAGATTGGATTGTCTAGCCATTCAT1090.10680306103451993No Hit
ACTCTTGGGCACAGAGGGCAAGTACATCATGTCGGACTTGGGCCCGCAGGAG1090.10680306103451993No Hit
CTTCCCGGGGGGCGGACCACTCATGGCGACAGGCGGCCAGCTTCCCTGGTAT1090.10680306103451993No Hit
ACAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1090.10680306103451993No Hit
GTGCGGGGAGCGGACAGTGGACTTAAAATACTTGCTTGTGATGACTTGGATG1080.10582321643787294No Hit
GCTAACGGGGGATTGCTCCTTGGGAGTTAAGATTGTCAATACTCCTGTGAAG1080.10582321643787294No Hit
CACAACCCTGAAGCACATACAAGCACCCTGGTGGAAGAAGCTTGGAGAAGAA1080.10582321643787294No Hit
ACAGTGGGAACATAGAAACTATAGATTAAGTGAATGAAAACAGCAAGTATGG1080.10582321643787294No Hit
CAACAAGGGATTAAAAAGTAACTTTAGCAATTTTACTAAATAACAAGGTTAA1080.10582321643787294No Hit
TATTAGGGGTCTTGTTTTTAGTCTCGCTTTTCGGTTGCCGTTGTCTTTTTTC1080.10582321643787294No Hit
GCCAAGGGGTCATATGTGAAGATGTCAATAAGCTTGCATTAAGCCACCTGCT1070.104843371841226No Hit
TGCAATGGGATTCCATCGAGATTTTAAGCAGCACCATCAAGCCTTACAATGC1060.10386352724457901No Hit
CATGAAAGCCAGCCTATTTAAAAGTGTTTAATATATTTTCATGAATGATTTA1060.10386352724457901No Hit
GAAGAAGGGGATCTAGTTAGCGGGAGGATCCTCTTATTAAGAAAAACAACAC1060.10386352724457901No Hit
GATACGGGAGTGGAAACTGGTTAAGCCAGTTGTTCATACTTCGTTTACAAAT1050.10288368264793203No Hit
TGCGTAGGGATCATTTATGCATTTTAAAAATCCTGAGCTTGTTACAATTCTT1050.10288368264793203No Hit
CTTGTGGGGCACAGTCTGGTGGTGACATGGCAGGGAATTTTACCTAAGATAA1050.10288368264793203No Hit
TTCATAGGGATGAATTGTGTGGTTAAAATTGATTTGGTATGTTTTAGTATGA1050.10288368264793203No Hit
CGAACCGGGATTTCAAGACCAAAGCAAAGTCTTACTACTACTGTGGAACCAT1050.10288368264793203No Hit
ATTAAAGGGGTGTTTCATTTCTTAGTCTAGGTGGAAGCTACACAGAAATGTG1050.10288368264793203No Hit
TCTACTGGGAACAAATAATATATACACTAATTTATGTATTTGTAATGTATGA1050.10288368264793203No Hit
TAGCGGGGGAATGATCTAGAATCTCAAATAAGGCAGGACATATCCAATGCTG1040.10190383805128507No Hit
GACACTGGGCATCCCCAGTGGAGGCTCTGAAAGGTCTGGTGGATAAGCTTCA1040.10190383805128507No Hit
GCCACTGGGATACTGAAAGAATCAAAAACAAATCATCCAAATTAAATTTCAA1040.10190383805128507No Hit
GCGGACGGGGGAAAGAAAATGATGTCTGTTGTTATAGTTCATTGTTTTGCCT1040.10190383805128507No Hit
GCTAATGGGTTTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC1040.10190383805128507No Hit
CGCTAAGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTC1040.10190383805128507No Hit
TGTCGTGGGAGAGGAGGACATTTGGGCTCACCTGTCAAGGTGGCCCTGGGAC1030.1009239934546381No Hit
GTGGCTGGGAGAAACATAACAAACAACTTGGCTTACTTTCTTCTGCGAAGCA1030.1009239934546381No Hit
GACAATGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1030.1009239934546381No Hit
AGTCATGGGATTAAGCAGTATTAAGAAAATGTAGCAATATTGAAATGATTTT1030.1009239934546381No Hit
CAGATCAACAACCTACTTTAATACTTCAGGATATGAAAGAAGAACAAACTAA1030.1009239934546381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)