FastQCFastQC Report
Sat 31 Dec 2022
SRR5844165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247902
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT11570.4667166864325419TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC10220.4122596832619342No Hit
AACCACGGGACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAACAGG3110.12545279989673339No Hit
GCTTTAGGGTCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGAC3000.12101556260135055No Hit
TTCACAGGGAGAGAATTCAATGTGTACAGGCAATTGTTTATTAAAATCCATC2620.10568692467184614No Hit
TTTCTGGGGATCAGATTAATGATCTTAGTCATGCAAATTTAATTTATAAAGT2580.10407338383716146No Hit
GTGTCGCGGGGTGGGTTAGCAGAGGGCAAATTGGAGGATTCCTTTGAGGAAA2580.10407338383716146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)