FastQCFastQC Report
Sat 31 Dec 2022
SRR5844164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247735
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT11540.465820332209821TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC10580.4270692473812744No Hit
AACCACGGGACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAACAGG2970.11988616868831616No Hit
TTCACAGGGAGAGAATTCAATGTGTACAGGCAATTGTTTATTAAAATCCATC2900.11706056875290127No Hit
GTGTCGCGGGGTGGGTTAGCAGAGGGCAAATTGGAGGATTCCTTTGAGGAAA2710.10939108321391809No Hit
TATAATGGGAGGGAGTGGAAGATGATGTTTTTTGAGTACCTATTATTTGCTA2700.10898742608028741No Hit
GTGCTCGGGATCTATAGGAAGATCCTAATTGCCATATTCACAAACACTAAGT2520.10172159767493491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)