FastQCFastQC Report
Sat 31 Dec 2022
SRR5844148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160299
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9220.5751751414544071No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT8430.5258922388785957TruSeq Adapter, Index 2 (95% over 22bp)
TCCCTTGGGGGATGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGA2130.13287668669174482No Hit
GACTATGGGGAACAGAGGGACCGGCTGGAGCTGAGGCAGAAGAACATAAAAT2110.131629018272104No Hit
CAAACTGGGTATAATAGCTTCTTTTAAATCCTTGCTGATTTCAACATCTGAG2100.13100518406228362No Hit
CATCACGGGGATGGGTCCTACTATTTTATCTTTAAGGTCAATTATTACAGCA2040.12726217880336121No Hit
AAGAGAGGGTGAACTAAAGTCTTGCTAAGTCTGAAAACCTGCCTTCAATCTA2010.1253906761739No Hit
TCATGGGGGAAACATAATTTGAAAGATGCTCTTGCAGGAAATTTTTCTTTGT1980.12351917354443884No Hit
GGAGCTGGGAACGCCAGAGTCTCAAGCGCGGTTCGGTCAGTCCGTGAAGGGG1970.12289533933461842No Hit
TTTACGCAGGGTTGAATGATAGCTAAAGACTGTTAGGATTCTAAAGCCAAAT1960.12227150512479804No Hit
GCACATGAAACCTTCTTCAGAATAGATCTCATATTAGGCCATAAAATCAGAC1940.12102383670515723No Hit
CTACCTGGGACTTACTATAAATTTTTAACTGTACATTATAATTACTTTTTTA1940.12102383670515723No Hit
TTGGTTGGGGAAACTCACATTAGCACGCTACCAGAACAGTTGTTGTGTGTAT1940.12102383670515723No Hit
TCATAGGGAGAATATTAAAACTGCTTTGCATGGGTTTTCTGGGAAATTAGGA1930.12040000249533683No Hit
AGCGATGGGCAACTACCATGGATGTTAATTGGAGAAACACTCAAAAACATGG1910.11915233407569605No Hit
TCCAGCGGGACATTAAAAAACTACTGGTTAAAATTTAGATTAGAACAATCTT1910.11915233407569605No Hit
AAGTGAGGGGTTTCTGTAGTTGTCTTCAGAAAACTTTATTAATCTTATTGGT1900.11852849986587563No Hit
AGTTGCGGGACAGAAGGACATAAAATGAATGATCAGGCATGGCCGTTGCCTT1900.11852849986587563No Hit
CTAATCGGGGTTGGAAGAACCAGTTCTGGAGTTCAGGCCTCCCCGAGGAGCA1900.11852849986587563No Hit
CAAGAAGGGCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCA1890.11790466565605524No Hit
TGTCAAGGGCATTATTATTATTTTGTGATAAGAACACATAAGATACACTCTC1870.11665699723641446No Hit
TCTCTTGGGAGGCATGAGCCACCACGCCCAGCCGTATTTTTAATTTTTACAT1870.11665699723641446No Hit
TCTAATGGGGAAAATAATAATGATAAATGCTGGAAATTAGGATGGGAACTTT1860.11603316302659404No Hit
GCTCTAGGGAGTGTACTTCATGAAGCAGACCATTGGGAATTCCTGTGGCACA1860.11603316302659404No Hit
TTAGCAAGGGGTATTTATCCAAATTGCTGTTCCTTCCTTGATCAATTGCTCC1860.11603316302659404No Hit
TTTTATGGGGAAAAAGAAAATTGGAAGCTGCAAGCAAATGTAGAATCCTTAA1840.11478549460695327No Hit
TGCACCGGGACCTTCAGAATAGCAATCTGGAACACTGAAAAAGCTTGCTTTA1840.11478549460695327No Hit
GTTACTGGGATATTTGTTTTATTTATTTTTTTTAAGAAGAAAAAAGTTCATC1840.11478549460695327No Hit
TGACGTGGGTGCTGCCTTCTTTGTTTTATGGGCACTTTTTGTGTGATTACTT1830.11416166039713285No Hit
TGTACCGGGAAGAAATGGGTGAAATAAAACATTTTTATAATGGGCTTTGTTC1830.11416166039713285No Hit
AGCTCGGGGGACTATGGTCATGGCGGAGGGGACGGCAGTGCTGAGGCGGAAC1820.11353782618731245No Hit
TTAGACCGGGATTAGTAAATTCTCAGAGTTTCTGAGACATATCAGAACTCCT1820.11353782618731245No Hit
TGTTCAGGGGGGGAGCCAGCGGTATGGCGTCGGGCTGCAAGATTGGCCCGTC1810.11291399197749206No Hit
TAGACGGGGATATAAAATCTGTATATCTTGCTCTCTCTCCCACTTTTAAAAA1800.11229015776767168No Hit
AGTTGTGGGGTCTACATTGCGTGTGTGGATGGATGAATGAGCAGTGGGAGTG1790.11166632355785126No Hit
ATACATGGGCTCAAAGGTATTCACATCACAATTTCTTCTAAAATTTTCAAGT1790.11166632355785126No Hit
CAACCTGGGTTCTGCCCTTGTCACAAGCTCCTAGGTGATGTTAACAATGCTG1790.11166632355785126No Hit
ACTCTAGGGTATGTGTGTGGAACTCTGACGTGTATTTTAAACTTTGCAATAG1780.11104248934803086No Hit
TTTCTAGGGGAGGAGAAGCTGCAGAAGCTGCACAGTGAGATCAAGTTTGCCC1780.11104248934803086No Hit
AGAAATGGGCGATCTTGTAACGGGAGCAAGGGCTGGGGAAAGGCAGAACACC1780.11104248934803086No Hit
TAACAAGGGAATCCACAGTCATTTCTCATAAGTTACGGTCTTCTTGCATTTG1760.10979482092839006No Hit
CTGCACGGGTCAGAGGGAATTGGAGGATATCCACAGTCTGTTCCTGTTCTGA1760.10979482092839006No Hit
GGACGCTGGGAGTTATAACAGCAACAAAGCGAGACCCCATCTTTACAAAAAA1760.10979482092839006No Hit
GGCAATGGGTATAAATTTTTATACCATTTATAAGTACTGTTATATTTTTGAA1750.10917098671856967No Hit
GAGAAAGGGCTTCGTACGGGTGGATGAAAATAATTCTCCACAATTTTAAGTA1750.10917098671856967No Hit
CAGAGGGGAGGAGTGCAGATAAGTACATAAGTTGGTGTTTGGATTGAGTATT1740.10854715250874927No Hit
GTCGCAGGGATGTAACAAATTGCATAAGCAGAAGAAATAAATTCCTAAAAAC1740.10854715250874927No Hit
GACATGGGGCAAATAAACAAACTTTCAGATCAATCAGCTAATAATAATAATG1730.10792331829892889No Hit
TCGTACGGGGAGTTGGCTGCCGGTGAGTTGGGTGCCGGTGGAGTCGTGTTGG1730.10792331829892889No Hit
ATTACTGGGATGCTCGATTTCCTCTGCCTCTCTCTTTGCCGAGCTTTCCGCG1730.10792331829892889No Hit
GTACAAGGGCAGTCTACAAAATACCTAAGTGATACTCTTCAAAAGTGTGTCA1710.10667564987928808No Hit
TTCACTAGGACTTAAAGGTTATTACACTCAGCTTCAAAATGATATTGAAATC1700.10605181566946768No Hit
GTTGCAGGGATCATTAATTGTTCTTAGTACAGTACACAGATTTAAAACTTAT1700.10605181566946768No Hit
ATTTTTGGGTGATCATATTCAATTCAGTTCAATTCTGTTTAAGAGATTTTCG1690.1054279814596473No Hit
GCCTATGGGTTAGTGACATAAGTAGCAGCCGTTTTTCAACTTCAGTTTCATT1680.10480414724982688No Hit
GTATGAGGGGAATACAGAATGGGTAGTAGAAGGTAGTCATCAATACCAGCCA1680.10480414724982688No Hit
GCCAGTGGGCAAGCCTGCTATGTTTACTGTTTTATGAATGTCAACATTCTCC1670.1041803130400065No Hit
TGCACCGGGCCAAGAGTTCGAGGCTTCAGTGAGCTGTGATTCACTACTGCAC1670.1041803130400065No Hit
TGTTAAGGGACTGATATGTATTCTTCAGTGATACATATGTACGAAGGAAAGT1670.1041803130400065No Hit
TTGCCGTGGGACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAGA1660.1035564788301861No Hit
GGGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.1035564788301861No Hit
GTTTCGGGGGGCCCCTGTGTTCATGAGTGAAAATGAGTTTAAGAAGGAACAG1660.1035564788301861No Hit
ATTTATGGGGCCTGTTCCTCTGTCTTCTCAATGAATAATGATTGTCAAGCCA1660.1035564788301861No Hit
ACAGATGGGAAATTAGTTAAAATTAAAAGCAAAAGATTAGAAAAATTAAAAC1650.10293264462036568No Hit
AGTATAGGGGTTCTTTGTGTATCATCTTATACGTTGCAGGTAATTTTATCCT1650.10293264462036568No Hit
GGCACTTTGGGAGGCCAAGGCAAATGGATCACCTGAGGTCAGGAGTTCAAGA1650.10293264462036568No Hit
ATCAATGGGATAGACTAACATGGATCTAACTTTTTGACTATCTTCCTCTTTT1650.10293264462036568No Hit
TTGACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.10230881041054529No Hit
ATACGAGGGAATTACTTTTGCAGCAGAGTACCATATCTGTTTCTTATCTGCA1640.10230881041054529No Hit
ACTTGCGGGAACTAGTACTATTAATTACACGGAGTATTGTTGTTAAAATGCA1640.10230881041054529No Hit
ATTGTTGAGGGGGGCTACTGTGGATAATGCTGCTGTGAACATGTGTATACAA1640.10230881041054529No Hit
TCACTTGGGCGATCTCTAGGAGGGGACCATAAAATGCATTTTATAATTTTGT1630.10168497620072489No Hit
GCCTATGGGAGAGGACGGGTCTAATAGATCGCTGGAGACACAATTTAACTGA1630.10168497620072489No Hit
CAACAGGGGATTATTCAAACAAGCTATATATATGTACAAAGGCAAACAGACA1630.10168497620072489No Hit
CCACCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1630.10168497620072489No Hit
AGCAATGGGGACGGTGGCCGAGAGGATGCCGCAGATGTGTTAGCGGCGAGTC1630.10168497620072489No Hit
GTAAGTGGGGACACTGAAGGTTTGGAGAGAAGATGGCTTTTATCCCTCAGAA1630.10168497620072489No Hit
TAAGCATGGGGGGATCATATTCCTTCATCTACCATGGCTCAGCCGGGAACTC1630.10168497620072489No Hit
CGCTTTGGGATGCCTGCAATCACACAGTCACTCAAACCTAAGCCTACTCTTC1620.1010611419909045No Hit
GAAACTGGGAGCTACTCACTCTGTTTACATTGGAGAAGGTTCAGGCATTTGC1620.1010611419909045No Hit
GTTGCAGGGGAGACAGCTGACCATGGAAGCGAATGGGTTGGGCCTCCAGCGT1620.1010611419909045No Hit
TGTCAGGGGATTCGGGAACTGACTGCTGTAGTTCAGAAGAGGTTTGGCTTTC1610.10043730778108409No Hit
CTGCAGGCATTTTCTTGTTTCTGTGCGATCAGGTTTTTACCCATGCAAAGTG1610.10043730778108409No Hit
TTAGAGGGGTGAGTGCAGATGGGAAGACCATCACTGTCACTTTCTTTAATGG1610.10043730778108409No Hit
AAAGCTGGGCATTTGAATGTTACAAATGTAACATATTACATTTGGATGTTAA1610.10043730778108409No Hit
TGCTCGTGGGTGATGGCAACAATATTCACTTTGCCAGAGTTAAAAGGAGCTC1610.10043730778108409No Hit
AAACTTGGGAGTAAAGAAAAGTTTGATTATGGTCTACTAATTGAAATTAGAA1610.10043730778108409No Hit
ATGCAGGGAATGAGTTCCGTTCTGGAAAGGCACCCATCCTTATTGCTACAGA1610.10043730778108409No Hit
TCGCTGGGGGAAGGAGCAGGAGGAAATCCAGGAGAGGGTGGGATCTGGAAGC1610.10043730778108409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)