FastQCFastQC Report
Sat 31 Dec 2022
SRR5844147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160732
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9070.564293357887664No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT8530.5306970609461712TruSeq Adapter, Index 2 (95% over 22bp)
GACTATGGGGAACAGAGGGACCGGCTGGAGCTGAGGCAGAAGAACATAAAAT2550.15864918000149317No Hit
AGTTGTGGGGTCTACATTGCGTGTGTGGATGGATGAATGAGCAGTGGGAGTG2250.1399845705895528No Hit
ATTACTGGGATGCTCGATTTCCTCTGCCTCTCTCTTTGCCGAGCTTTCCGCG2190.13625164870716472No Hit
AAGTGAGGGGTTTCTGTAGTTGTCTTCAGAAAACTTTATTAATCTTATTGGT2180.13562949506010002No Hit
TCCAGCGGGACATTAAAAAACTACTGGTTAAAATTTAGATTAGAACAATCTT2130.13251872682477664No Hit
TCATAGGGAGAATATTAAAACTGCTTTGCATGGGTTTTCTGGGAAATTAGGA2090.13003011223651792No Hit
TGTCAAGGGCATTATTATTATTTTGTGATAAGAACACATAAGATACACTCTC2090.13003011223651792No Hit
AGCGATGGGCAACTACCATGGATGTTAATTGGAGAAACACTCAAAAACATGG2070.1287858049423886No Hit
GCTCTAGGGAGTGTACTTCATGAAGCAGACCATTGGGAATTCCTGTGGCACA2040.12691934400119453No Hit
AGTATAGGGGTTCTTTGTGTATCATCTTATACGTTGCAGGTAATTTTATCCT2040.12691934400119453No Hit
TTTCTAGGGGAGGAGAAGCTGCAGAAGCTGCACAGTGAGATCAAGTTTGCCC2010.12505288306000048No Hit
AGAAATGGGCGATCTTGTAACGGGAGCAAGGGCTGGGGAAAGGCAGAACACC1970.12256426847174179No Hit
CATCACGGGGATGGGTCCTACTATTTTATCTTTAAGGTCAATTATTACAGCA1970.12256426847174179No Hit
GCACATGAAACCTTCTTCAGAATAGATCTCATATTAGGCCATAAAATCAGAC1960.12194211482467711No Hit
ATACATGGGCTCAAAGGTATTCACATCACAATTTCTTCTAAAATTTTCAAGT1950.12131996117761241No Hit
AGTTGCGGGACAGAAGGACATAAAATGAATGATCAGGCATGGCCGTTGCCTT1910.11883134658935371No Hit
GGCACTTTGGGAGGCCAAGGCAAATGGATCACCTGAGGTCAGGAGTTCAAGA1900.11820919294228903No Hit
TTAGACCGGGATTAGTAAATTCTCAGAGTTTCTGAGACATATCAGAACTCCT1900.11820919294228903No Hit
TCTCTTGGGAGGCATGAGCCACCACGCCCAGCCGTATTTTTAATTTTTACAT1890.11758703929522435No Hit
TCATGCGGGAATGACTATCAATTCACTTCAACAAAATACATACCTGTGGTAT1890.11758703929522435No Hit
TTCACTAGGACTTAAAGGTTATTACACTCAGCTTCAAAATGATATTGAAATC1880.11696488564815967No Hit
CGCTTTGGGATGCCTGCAATCACACAGTCACTCAAACCTAAGCCTACTCTTC1880.11696488564815967No Hit
ATACGAGGGAATTACTTTTGCAGCAGAGTACCATATCTGTTTCTTATCTGCA1880.11696488564815967No Hit
CTACCTGGGACTTACTATAAATTTTTAACTGTACATTATAATTACTTTTTTA1880.11696488564815967No Hit
GACATGGGGCAAATAAACAAACTTTCAGATCAATCAGCTAATAATAATAATG1870.11634273200109499No Hit
CTAATCGGGGTTGGAAGAACCAGTTCTGGAGTTCAGGCCTCCCCGAGGAGCA1860.11572057835403031No Hit
GAAGCTGGGCTCAGATTCTTTGCTTAAGTTTTAATTGGGTTACAAATCTTTT1860.11572057835403031No Hit
TGCACCGGGCCAAGAGTTCGAGGCTTCAGTGAGCTGTGATTCACTACTGCAC1850.11509842470696563No Hit
TCACTTGGGCGATCTCTAGGAGGGGACCATAAAATGCATTTTATAATTTTGT1840.11447627105990095No Hit
TCCCTTGGGGGATGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGA1840.11447627105990095No Hit
TGAACTTCCCAGCCTCCAGAACTGTGAGCAATAAATTTCTGTTGTTTATAAA1840.11447627105990095No Hit
TGGTGAAGGGATCGGAATTTGAGGATTTGGAGATTTCAGGCTCCAGGGATTG1840.11447627105990095No Hit
GCGTAAGGGAGGAGTTTAGTGGCTTTCTACTCCCACCCCACTTCCTGAGGAA1820.1132319637657716No Hit
ACTCTAGGGTATGTGTGTGGAACTCTGACGTGTATTTTAAACTTTGCAATAG1810.11260981011870692No Hit
CTGCACGGGTCAGAGGGAATTGGAGGATATCCACAGTCTGTTCCTGTTCTGA1810.11260981011870692No Hit
GGAGCTGGGAACGCCAGAGTCTCAAGCGCGGTTCGGTCAGTCCGTGAAGGGG1800.11198765647164224No Hit
TTGGCTGGGCAAATGGATATTTATAGGAATATATTCACTACTTTGTATACTT1790.11136550282457756No Hit
TCGCTGGGGGAAGGAGCAGGAGGAAATCCAGGAGAGGGTGGGATCTGGAAGC1780.11074334917751287No Hit
CAACCTGGGTTCTGCCCTTGTCACAAGCTCCTAGGTGATGTTAACAATGCTG1780.11074334917751287No Hit
AGCAAAGGGTAACATTGAATGTAAATGGCCTAAATGTTCCTCTTAAAAGATA1760.10949904188338351No Hit
GTACAAGGGCAGTCTACAAAATACCTAAGTGATACTCTTCAAAAGTGTGTCA1760.10949904188338351No Hit
GTGGTTGGGCACCTTCTGAGTATTCTCATAGGAGACTGCGTCAAGCAATCGA1760.10949904188338351No Hit
GTATGAGGGGAATACAGAATGGGTAGTAGAAGGTAGTCATCAATACCAGCCA1760.10949904188338351No Hit
GAGAAAGGGCTTCGTACGGGTGGATGAAAATAATTCTCCACAATTTTAAGTA1760.10949904188338351No Hit
CAAACTGGGTATAATAGCTTCTTTTAAATCCTTGCTGATTTCAACATCTGAG1760.10949904188338351No Hit
AAGAGAGGGTGAACTAAAGTCTTGCTAAGTCTGAAAACCTGCCTTCAATCTA1750.10887688823631883No Hit
TGTACCGGGAAGAAATGGGTGAAATAAAACATTTTTATAATGGGCTTTGTTC1750.10887688823631883No Hit
GTCGCAGGGATGTAACAAATTGCATAAGCAGAAGAAATAAATTCCTAAAAAC1750.10887688823631883No Hit
TTGGTTGGGGAAACTCACATTAGCACGCTACCAGAACAGTTGTTGTGTGTAT1740.10825473458925416No Hit
ATTTTTGGGTGATCATATTCAATTCAGTTCAATTCTGTTTAAGAGATTTTCG1740.10825473458925416No Hit
AACACCGGGATCTAGGCGTGGGAGTAGACAAATGCAGCAGCAAGTCAATTGT1730.10763258094218948No Hit
GCCTATGGGAGAGGACGGGTCTAATAGATCGCTGGAGACACAATTTAACTGA1730.10763258094218948No Hit
TGCACCGGGACCTTCAGAATAGCAATCTGGAACACTGAAAAAGCTTGCTTTA1730.10763258094218948No Hit
CAAGAAGGGCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCA1730.10763258094218948No Hit
TGCATAGGGGAGCGGCCAGCGCTGGGAACTGGCACTGGGTCGCTTTTGGGAT1730.10763258094218948No Hit
GTTGCAGGGATCATTAATTGTTCTTAGTACAGTACACAGATTTAAAACTTAT1720.1070104272951248No Hit
TGTTAAGGGACTGATATGTATTCTTCAGTGATACATATGTACGAAGGAAAGT1720.1070104272951248No Hit
GTTGCAGGGAGAACAAATATGCATAAACGTTAATCAGTTTCTCTATACAAAA1710.10638827364806012No Hit
GTGCTGGGGACAAAAAAATAGGAAGACGAAGCATTTGGAGAAATTAGGTTGG1700.10576612000099544No Hit
TAGACTGGGGGGATTAGTTATCTGATTCATATGTTTCAGTAAAGAGATGGTT1700.10576612000099544No Hit
CCGCTCGGGTGCTCCCCCTTCTTCATATACACACACTGAAATCACTCCACTC1700.10576612000099544No Hit
CTGCGTGGGGAGATTTAATTTAAAAGAAAAATAGAATACATATAAAAATTTT1700.10576612000099544No Hit
TCGCCGGGGTGGTACAAGAACTTCACTTGACATTGAAGAGTACTCGGATACT1690.10514396635393077No Hit
TGCTCGTGGGTGATGGCAACAATATTCACTTTGCCAGAGTTAAAAGGAGCTC1680.10452181270686609No Hit
AAATCGTGGGAGAACCATAACTTACACATGAAAGTCATATACTAGATCCAAT1670.1038996590598014No Hit
ACAGATGGGAAATTAGTTAAAATTAAAAGCAAAAGATTAGAAAAATTAAAAC1660.10327750541273674No Hit
GTGCAGGGATGGACGAAATGTAACCTCAGAGAAACAACTACAGGACCACTCA1660.10327750541273674No Hit
ATCCTGGGGAGGCCAGGCTACCAACGTCTTTGTGAACATGGAGGAGGACTTC1660.10327750541273674No Hit
GACCATGGGAAAATAATGTAATTAAAAATCATATAGTCTTATTCATCTATAA1650.10265535176567206No Hit
CGGGTTGGGTCTGCAATTCAGCTTTCTGGAAGTATTATAGAAAAAGAAGGTT1650.10265535176567206No Hit
AAACTTGGGAGTAAAGAAAAGTTTGATTATGGTCTACTAATTGAAATTAGAA1650.10265535176567206No Hit
TTTACGCAGGGTTGAATGATAGCTAAAGACTGTTAGGATTCTAAAGCCAAAT1650.10265535176567206No Hit
TTAGCAAGGGGTATTTATCCAAATTGCTGTTCCTTCCTTGATCAATTGCTCC1650.10265535176567206No Hit
GTAAGCGGGATTGAGAATGAAACAATGAACACAATAAGGTGATATTCCTACT1640.10203319811860738No Hit
GGGTGTGGGAGCCAAGCCACTCCATGGCAATGAGCTCCGAAGACAAAGTGCA1640.10203319811860738No Hit
GTGTGTGGGACCACTGTGGAAGTCCCTCAGAGGCCTTGGGGCATGACCCAGT1640.10203319811860738No Hit
TGTCAGGGGATTCGGGAACTGACTGCTGTAGTTCAGAAGAGGTTTGGCTTTC1630.1014110444715427No Hit
AAAGTGGGCAGTGTTGTTAGAAGTGTATATAATCTGAATCAATAAGCTCTGA1630.1014110444715427No Hit
GTTTCGGGGGGCCCCTGTGTTCATGAGTGAAAATGAGTTTAAGAAGGAACAG1630.1014110444715427No Hit
TAACCGGGGGTCAGTATTTTGTTTCTAAGGTGGTTAAATGTGTTTTTAAACT1630.1014110444715427No Hit
CTATAAGGGTCGCTATACTGCACTTACGCTATCCAGTTGCAGGTAATTTTTT1620.10078889082447802No Hit
CATCCTGGGGCAACACTGCCTACAAAGCACAGTGGTAACTTCTAACTTTCCT1620.10078889082447802No Hit
TAACAAGGGAAGAAACCCAAATGGGATGACTTCAAAAAGAAAAAGAAAGAAC1620.10078889082447802No Hit
TGGTTCGGGTTAGGCAATGAATTGACTGTATGTCTTAATTAGTTTCTAAGAC1620.10078889082447802No Hit
ATTCTTGGGCAACCATGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGT1620.10078889082447802No Hit
TAGCATGGGAAGAATGGGGTGGAAGGATGCCAGAGGAAGAAAAGGAAATGTG1620.10078889082447802No Hit
TCAAATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1610.10016673717741333No Hit
TAGACGGGGATATAAAATCTGTATATCTTGCTCTCTCTCCCACTTTTAAAAA1610.10016673717741333No Hit
ATGCAGGGAATGAGTTCCGTTCTGGAAAGGCACCCATCCTTATTGCTACAGA1610.10016673717741333No Hit
GGAGTAGGGAATCTGATTCTACCTAATTTTTAAACCATTTCTAAAGAGAAAG1610.10016673717741333No Hit
TCACACGGGCAAATCAATCAGTGTAATACATTGTTTCAACAGAATGAAGAAT1610.10016673717741333No Hit
TCATGCGGGGATCTGCGTACGGGGGCAGCCAGGTGTAGTTTCGGGAGCCACT1610.10016673717741333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)