FastQCFastQC Report
Sat 31 Dec 2022
SRR5844146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158535
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC8910.5620210048254329No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT8150.5140820638975621TruSeq Adapter, Index 2 (95% over 22bp)
AAGTGAGGGGTTTCTGTAGTTGTCTTCAGAAAACTTTATTAATCTTATTGGT2170.13687829185984168No Hit
TTGGTTGGGGAAACTCACATTAGCACGCTACCAGAACAGTTGTTGTGTGTAT2140.13498596524426784No Hit
GCACATGAAACCTTCTTCAGAATAGATCTCATATTAGGCCATAAAATCAGAC2100.13246286309016939No Hit
CTAATCGGGGTTGGAAGAACCAGTTCTGGAGTTCAGGCCTCCCCGAGGAGCA2070.13057053647459552No Hit
CTAACAGGGCACTGTTAAATTGAATCAAGTATTTCTCTATATGCTTTTGTAT2000.12615510770492322No Hit
AGTATAGGGGTTCTTTGTGTATCATCTTATACGTTGCAGGTAATTTTATCCT1960.12363200555082475No Hit
TCCCTTGGGGGATGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGA1950.12300123001230012No Hit
ATACATGGGCTCAAAGGTATTCACATCACAATTTCTTCTAAAATTTTCAAGT1940.12237045447377551No Hit
TGCACCGGGACCTTCAGAATAGCAATCTGGAACACTGAAAAAGCTTGCTTTA1930.12173967893525088No Hit
GTCTTTGGGACATGTAATGTGAGCAACCATAACTTTCACTGAACTTGTGTAA1930.12173967893525088No Hit
TCATAGGGAGAATATTAAAACTGCTTTGCATGGGTTTTCTGGGAAATTAGGA1920.12110890339672627No Hit
GTATGAGGGGAATACAGAATGGGTAGTAGAAGGTAGTCATCAATACCAGCCA1910.12047812785820167No Hit
TGTACCGGGAAGAAATGGGTGAAATAAAACATTTTTATAATGGGCTTTGTTC1900.11984735231967704No Hit
CAACCTGGGTTCTGCCCTTGTCACAAGCTCCTAGGTGATGTTAACAATGCTG1900.11984735231967704No Hit
TTGCATGGGAATTTATCAATATTATAAATTGATTAAATTTACGAATTTATAA1890.11921657678115243No Hit
AGCTCGGGGGACTATGGTCATGGCGGAGGGGACGGCAGTGCTGAGGCGGAAC1880.1185858012426278No Hit
TTTACGCAGGGTTGAATGATAGCTAAAGACTGTTAGGATTCTAAAGCCAAAT1880.1185858012426278No Hit
GCTCTAGGGAGTGTACTTCATGAAGCAGACCATTGGGAATTCCTGTGGCACA1840.11606269908852934No Hit
GTGGTTGGGCACCTTCTGAGTATTCTCATAGGAGACTGCGTCAAGCAATCGA1840.11606269908852934No Hit
ATTACTGGGATGCTCGATTTCCTCTGCCTCTCTCTTTGCCGAGCTTTCCGCG1840.11606269908852934No Hit
TAACAAGGGAATCCACAGTCATTTCTCATAAGTTACGGTCTTCTTGCATTTG1820.11480114801148011No Hit
GTTGCAGGGAGAACAAATATGCATAAACGTTAATCAGTTTCTCTATACAAAA1820.11480114801148011No Hit
CATCACGGGGATGGGTCCTACTATTTTATCTTTAAGGTCAATTATTACAGCA1820.11480114801148011No Hit
AGCAAAGGGTAACATTGAATGTAAATGGCCTAAATGTTCCTCTTAAAAGATA1800.11353959693443089No Hit
TTGGCTGGGCAAATGGATATTTATAGGAATATATTCACTACTTTGTATACTT1800.11353959693443089No Hit
GTTACTGGGATATTTGTTTTATTTATTTTTTTTAAGAAGAAAAAAGTTCATC1790.11290882139590626No Hit
CTGCACGGGTCAGAGGGAATTGGAGGATATCCACAGTCTGTTCCTGTTCTGA1790.11290882139590626No Hit
AGCGATGGGCAACTACCATGGATGTTAATTGGAGAAACACTCAAAAACATGG1780.11227804585738166No Hit
CATGCAGGGCAAACTGCTCTGTGCTTTCAAACCAAAGTGTTCCCCCCAACCC1780.11227804585738166No Hit
TTCACTAGGACTTAAAGGTTATTACACTCAGCTTCAAAATGATATTGAAATC1770.11164727031885704No Hit
TGACGTGGGTGCTGCCTTCTTTGTTTTATGGGCACTTTTTGTGTGATTACTT1760.11101649478033242No Hit
GGGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1760.11101649478033242No Hit
AGAGGTGGGATTCTCAGGACCTTCAGACATCTGTATCTCCTGTGGCCAAGGC1760.11101649478033242No Hit
GGAGCTGGGAACGCCAGAGTCTCAAGCGCGGTTCGGTCAGTCCGTGAAGGGG1760.11101649478033242No Hit
AAAGCTGGGCATTTGAATGTTACAAATGTAACATATTACATTTGGATGTTAA1730.10912416816475858No Hit
ATACGAGGGAATTACTTTTGCAGCAGAGTACCATATCTGTTTCTTATCTGCA1730.10912416816475858No Hit
TTGGCAAGGGAGTCACTCCCAAAGTTGTTGAAAAATTGACAGTCTGTAGTTG1730.10912416816475858No Hit
ACTACAGGGCTTTTGAAAGGCTGTAGACAGTGTGGCTCCCCTTCTGATTCAG1730.10912416816475858No Hit
GGCACTTTGGGAGGCCAAGGCAAATGGATCACCTGAGGTCAGGAGTTCAAGA1730.10912416816475858No Hit
CAAGAAGGGCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCA1730.10912416816475858No Hit
TCATGGGGGAAACATAATTTGAAAGATGCTCTTGCAGGAAATTTTTCTTTGT1730.10912416816475858No Hit
TCATGCGGGGATCTGCGTACGGGGGCAGCCAGGTGTAGTTTCGGGAGCCACT1730.10912416816475858No Hit
TAGACGGGGATATAAAATCTGTATATCTTGCTCTCTCTCCCACTTTTAAAAA1720.10849339262623396No Hit
TCACACGGGCAAATCAATCAGTGTAATACATTGTTTCAACAGAATGAAGAAT1720.10849339262623396No Hit
TAGACTGGGGGGATTAGTTATCTGATTCATATGTTTCAGTAAAGAGATGGTT1710.10786261708770933No Hit
GTACAAGGGCAGTCTACAAAATACCTAAGTGATACTCTTCAAAAGTGTGTCA1710.10786261708770933No Hit
TGCACCGGGCCAAGAGTTCGAGGCTTCAGTGAGCTGTGATTCACTACTGCAC1710.10786261708770933No Hit
TCCTATGGGAGTTGGAATCCAATCTTCTTCCCAAGCACCAAAAACATCTAAA1700.10723184154918472No Hit
ATGCAGGGAATGAGTTCCGTTCTGGAAAGGCACCCATCCTTATTGCTACAGA1700.10723184154918472No Hit
GGGTGTGGGAGCCAAGCCACTCCATGGCAATGAGCTCCGAAGACAAAGTGCA1700.10723184154918472No Hit
GTATACGGGGCTGGCCGGGAGCCCCTCCCCGGGGAGTTCTTGGTGAAGTCCT1700.10723184154918472No Hit
TCCAGCGGGACATTAAAAAACTACTGGTTAAAATTTAGATTAGAACAATCTT1700.10723184154918472No Hit
AGAAATGGGCGATCTTGTAACGGGAGCAAGGGCTGGGGAAAGGCAGAACACC1690.10660106601066012No Hit
GGACGCTGGGAGTTATAACAGCAACAAAGCGAGACCCCATCTTTACAAAAAA1690.10660106601066012No Hit
TGTCAAGGGCATTATTATTATTTTGTGATAAGAACACATAAGATACACTCTC1680.10597029047213549No Hit
GGCAATGGGTATAAATTTTTATACCATTTATAAGTACTGTTATATTTTTGAA1680.10597029047213549No Hit
GTTGCAGGGGAGACAGCTGACCATGGAAGCGAATGGGTTGGGCCTCCAGCGT1680.10597029047213549No Hit
ACAGATGGGAAATTAGTTAAAATTAAAAGCAAAAGATTAGAAAAATTAAAAC1670.10533951493361088No Hit
AGTTGCGGGACAGAAGGACATAAAATGAATGATCAGGCATGGCCGTTGCCTT1670.10533951493361088No Hit
TGTTCAGGGGGGGAGCCAGCGGTATGGCGTCGGGCTGCAAGATTGGCCCGTC1660.10470873939508625No Hit
AAAGTGGGCAGTGTTGTTAGAAGTGTATATAATCTGAATCAATAAGCTCTGA1660.10470873939508625No Hit
GCTTTGGGGCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCAT1650.10407796385656164No Hit
TAATATGGGGAGTGGGCAAAATGTTGATTATTTTCTAATGCTTTGTAGCAAA1650.10407796385656164No Hit
GAAGCTGGGCTCAGATTCTTTGCTTAAGTTTTAATTGGGTTACAAATCTTTT1650.10407796385656164No Hit
TCACCTGGGCAATTCATAAACAAATAAATGGCTTCAAAATTGTATGCTTTTT1640.10344718831803704No Hit
GACTATGGGGAACAGAGGGACCGGCTGGAGCTGAGGCAGAAGAACATAAAAT1640.10344718831803704No Hit
ATTAAAGGGAACCAATGACAAGCCTTGATGTTTATAACCTTTACTAAGAGAT1640.10344718831803704No Hit
TCTCTTGGGAGGCATGAGCCACCACGCCCAGCCGTATTTTTAATTTTTACAT1630.10281641277951241No Hit
AGTTGTGGGGTCTACATTGCGTGTGTGGATGGATGAATGAGCAGTGGGAGTG1630.10281641277951241No Hit
TGAACTTCCCAGCCTCCAGAACTGTGAGCAATAAATTTCTGTTGTTTATAAA1630.10281641277951241No Hit
ACACTTGTAAGTGAGAACATGCAGTATTTTCTAGTATTTGGTTTTTTGTTCC1630.10281641277951241No Hit
AAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1630.10281641277951241No Hit
GTTTAGGGGAATTTCCAGGTCACATAAAATTAGGAGAGATGACTAATACTAT1620.1021856372409878No Hit
AGCGTTGGGATCTGGAAAGATTCTAAAATATAATAGAAAATAAATTGAATAA1620.1021856372409878No Hit
GCCTATGGGAGAGGACGGGTCTAATAGATCGCTGGAGACACAATTTAACTGA1610.10155486170246317No Hit
ACTCTAGGGTATGTGTGTGGAACTCTGACGTGTATTTTAAACTTTGCAATAG1610.10155486170246317No Hit
TGGGCCGGGGTGTTCAATGGGCTTCAGGAATGAGTTGCATTCAGTGATGGAA1610.10155486170246317No Hit
CATCCTGGGGCAACACTGCCTACAAAGCACAGTGGTAACTTCTAACTTTCCT1600.10092408616393857No Hit
TTTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10092408616393857No Hit
TGTCAGGGGATTCGGGAACTGACTGCTGTAGTTCAGAAGAGGTTTGGCTTTC1590.10029331062541395No Hit
TTGACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1590.10029331062541395No Hit
TATAAAGGGATGGAATACAGTGTTCAGCTGCCTACTATTTTCCCATGCAGAT1590.10029331062541395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)