FastQCFastQC Report
Sat 31 Dec 2022
SRR5844142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151127
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC6110.4042957247877613No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT4900.32423061398691166TruSeq Adapter, Index 2 (95% over 22bp)
CTTGAGGGGGGGTTTTCCTGTAAGTCGTATTAATAATTCTCTAAGGGGACCC2370.15682174594877157No Hit
GAGTTGGGTGAGAAGCAGCTTCCATCTTCAACCTCGGATGATCGGGTAAAAG2350.1554983556876005No Hit
TATGTTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG2300.15218988003467282No Hit
AGCTATGGGCGATCTTGTTCGGGGGACCAAATTGCAGACTAATTGTTTCCAT2260.1495430995123307No Hit
AGACCAGGCTATGAAGTGTTCTTGGAAACTAGGATGGAAGAAATAGGCCACA2230.1475580141205741No Hit
TGCGTTTGGGGTTCCTTCTTGTAAGCACACACGTCAGGTTGAACTCTGACCT2200.14557292872881747No Hit
CTAATAGGGCTACCCCTTCACTATTTCTTTATTTCTAACTCTTTTCCATTGT2160.14292614820647534No Hit
CGCTAAGGGGACCCTACTGTAATAGGATTCTCTGATTTCTATATTTCAGAAT2090.1382942822923766No Hit
ATCAGCGGGTGTCCCTGCACAGCCCATCACCACCACCTTTTTGGAGAGACAT2090.1382942822923766No Hit
AGCGATGGGGGAAGAACGAGAGGGGGAGAGATGTAAGAAAAGAAAGGAAAAG2080.13763258716179108No Hit
TGAAGGGATCCTCCACATGGGAGGTAAATTGTTGAAACCTTGCATGACTGAA2050.13564750177003446No Hit
ATGCTTGGGAAATGTGCATATGTCACAAGAATTCTGTGAATATATTAAATCA2040.13498580663944892No Hit
AAAAAAGGGGCTTTATTAGGAAAAAAAATCAGCTCCAGAATACTCTGCTAAC2020.1336624163782779No Hit
ACTTCAGGGCAGACCCCAGGCTTAAGTGATGATGAGTTGGCCAGCTTGGTTA2010.13300072124769236No Hit
TCTGATGGGTCCTCTCTTCTGTAGGAGCTTTGGGTTTTCAGAGGTGCAGGGA2010.13300072124769236No Hit
CTCCACGGGGGTTTCTGCGGCGGCTGGAGAGGTGGTCGGAGAAGTAGGAACC2000.1323390261171068No Hit
ACTGGAGGGGATACATTCAGATCGGTAATAGCATGTTGAAAAAGAATGTGTG1990.13167733098652126No Hit
CTACTTGAGGGGATTTACACCTGAAACTAAATACAGAATGTTCTATCTCTAC1990.13167733098652126No Hit
TTGCTAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG1980.13101563585593573No Hit
CCAACCTGGGCGACAGAGCGAGACTCTGTCTCCAAAACAATAAATAAATAGA1980.13101563585593573No Hit
AGAATGGGGGGTTAATTCCCATTGGTGTCTTTGCTTATAGCATTTTTTCTCT1960.12969224559476467No Hit
CGATCGGGGGACGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCCATGGAG1950.12903055046417913No Hit
GGGTCCGGGTGAGACTGACATATGCCATTATCTCTTTTCCAGTATTAAACAC1950.12903055046417913No Hit
TGCACAGAGGACAGAAACTATAAAAGTAAGCAAACGAAAATGGAAATAATGA1950.12903055046417913No Hit
GCGAGTGGGTCCGATCTTTTGCCGGGAATGATAAGAGCAAAATTTGTGGAGT1940.1283688553335936No Hit
TCGCTGGGGGATCCACTTTTAATTCCTGAGGTTCCATGAGGAAAACAGAGGT1940.1283688553335936No Hit
CACATAGGGATTCACACACAAACATGACAGAAAAGTCATGAAGACAATAATT1930.12770716020300807No Hit
TTTGTTGGGGAGTAACAAGTTAGTGACTGGGTTAGGGGGTGGTTAGGACAGT1930.12770716020300807No Hit
TTAGTCGGGGACGCTCTTCCGATCTTCGATGGGGTCCTGTGGCAGTGGCGAT1930.12770716020300807No Hit
AGAACGGGCATCAGAATCACGCTCCAGATCAAAGTCAGCTTCAAAAACCCGA1930.12770716020300807No Hit
TACAGTGGGAGGCTGTATCTTCTGCCACTGTAATAAAATAGATGCAATTGAG1920.12704546507242254No Hit
TTGGCTGGGAGAGAATAATATACTTATTTTTAATGAAAACAAGAAAGTTATG1920.12704546507242254No Hit
TCTTCTGGGATCCGGCAGAGGGAGGAAGGCAGAGCAAGTGGAGCAGGGCTTA1920.12704546507242254No Hit
AGCTGTGGGCACACTCCAAAATTGAGCTATAGAGAGAAACAATTTTGGAGCT1900.12572207481125147No Hit
AGCTCGGGGATCCTTGCTGGGGACTGAAAACGCTTCTGTGAGACTTGATAAT1890.12506037968066594No Hit
TTACCTGGGGTGAGTTAATACTTTAGAAAAAAATCCATCAACTCAGCCCCGT1880.12439868455008041No Hit
GCGCGGGGCAGGGACTAACAATGTTAATCTGATAAGGACAGCAAAATCATCA1880.12439868455008041No Hit
GAAATTGGGTGCCCACTTGCCCAGGTCTAATAAACACTAAAGGGGGGAAATT1870.12373698941949486No Hit
TTTAACGGGCACTGAAGTGGCATCCAGATAAAAATCCTGAGAATAAAGAAGA1870.12373698941949486No Hit
GCGTATGGGGAACTATTTCATAATGCTATGCTTTCTATGGCAAAGAAAATAG1870.12373698941949486No Hit
GGAACGGGGATGGCAGAATACAGAAAATGCTTTATTGGAGACAGTCATTGTA1870.12373698941949486No Hit
ATACAAGGGGTGCTAGTTACTCTAAGAAAAACACCAAGTCTCAGTGGCTTCA1850.1224135991583238No Hit
GCTGCTGGGCAGAGGAAGTTTGTTTTTTAATTTAAGAGGGAAATATAACCTA1830.12109020889715272No Hit
AATTTTGGGGATCCCACATCTCTTTCAAAAGGTGATAATTAGAGTGAAGTTA1810.11976681863598165No Hit
TCATCGGGGTTTTATAAAGACTTCATTGATAAGATGGCTTTTATTCATACTA1800.11910512350539612No Hit
CGTTAAGGGCTAGTTGGTGTGCAGTGGTATCTTGTTGTTTTAATTAGCATTT1800.11910512350539612No Hit
ATTTAGGGGATCACTAGGCAATTCTGCTTTCTAAGCAGTTGTATTGAAAACA1790.11844342837481057No Hit
ACTACTGGGACCAAGGATGCAGCTTGACTTTAAATCCATTCATGTTCAAGAA1780.11778173324422507No Hit
CATGGGGTGGAGCCCCGGGGAGGGCAAGGCGACGGCCTCGCCCTTGTGCTGA1780.11778173324422507No Hit
ACACGGGGGGGGCGCATGAAGGTGGGGGTGATGCCGAAGGGGAAGGGATCGC1780.11778173324422507No Hit
TGCGAGGGATGGCTTATGAGAGAAGCATTTCTTAAATCTGGAAAACAAAACA1780.11778173324422507No Hit
TTTGCCCGGGTATGTAGAATTTTCATGGTTATTTTGTATGAATTATTTTTCT1780.11778173324422507No Hit
ATACCCGGGGATTGGCTCCAGGGAGGCATGATGCCACCGGGCAGCTCTTTTT1780.11778173324422507No Hit
CATGTTGGGTCTCAGATGAGAGGCTTGAATTTGATGGCCAGATATAACCTCT1780.11778173324422507No Hit
CATAATGGGAGTTCTTAGAGGAAGGAACATTAAGAGTAACAATCACAATGCC1780.11778173324422507No Hit
TGTCACGGGGCAGAGTTCAGACTTAGGAGTAAAACAGAAAAGTGAACTAAAC1760.11645834298305399No Hit
AGCAGCACTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTA1760.11645834298305399No Hit
AGTTGCGGGCTATCAGGGCGCTGTCAGACTGGCTGCTGGAGCCCAGATGGCA1760.11645834298305399No Hit
GGAGCCGGGCAAGTCACTAACCTTCCTCTTTGATCTCTTTGTAGGCTGACCA1750.11579664785246846No Hit
GTTTATGGGTTTTCAACTACTTTCTCTACAGCTTTTCATGTAAATTAGTCTT1750.11579664785246846No Hit
ACTTATGGGCTGCTGTAAATATACTTAATAAGAAGGCTAACTAGGCAACCTA1750.11579664785246846No Hit
CTTTAAGGGGGCGTAAGTAAGTGAGTCTAGCAGAAAGCTCTATTTCATCAAA1730.11447325759129738No Hit
CTGTTAGGGAACTGTTCCCATTTAGAGCCACCAGTTTCCAGGCTTGAAGGTG1730.11447325759129738No Hit
GACCGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1730.11447325759129738No Hit
TTAGTTGGGGGATTTGAAAATGTATATGGCCTAGGAATTATTGAAATTATTT1730.11447325759129738No Hit
CACAACGGGGATGAGCTGGCTGGGAAAATGAAAGACCTGAGTTTGGGAGGTG1730.11447325759129738No Hit
AAGCCGGGGATGGATTGAACTGTGTGCCCCTAAAAGATATGTTGAAGCCTGA1730.11447325759129738No Hit
TGTATAGGGTCTCGTGTCATGTATTACATAGCAGGTTTTTGGCAGAGACTGC1720.11381156246071185No Hit
ATCCGTGGGTACTACTAATTTGACACTTCTGAGTAGTGAAATGAAGTGTTTT1720.11381156246071185No Hit
GAAAATGGGCTACTTAAGTGGAAAGTGACTGAAAGATCTGGAAAAGTGGCTT1720.11381156246071185No Hit
TGACATTGGGAGAAGAGTATTAATATGAAATAATGAATAAGGACATTAAAAG1710.11314986733012632No Hit
CACCACGGGCGATCTGATTTGGGGGCCATGTTTCTGTCCCTGGTGTTCCTGG1710.11314986733012632No Hit
GAGGTTGGGGTGACGTTTTAGTCCCAGACTGAATTCCAAATTTGCTCTAGTT1710.11314986733012632No Hit
AAATAGGGGTTCATGGAATCTTCAAGGAAAACCAAATATTCTTTTTTAATAA1700.11248817219954078No Hit
TTGCTAGGGAGTCTTCCTCGATCCCGTGGTGCTCCGCGGCGCGGCCTTGCTC1700.11248817219954078No Hit
AAGTTTGGGATTGTTGTTTATAATGACAAATTTTTGGGAAAATCCTACATCT1700.11248817219954078No Hit
ACTCCCGGGTATTAGAGCAATTCATATAGAGATATGAAAATCGTTATTAGAT1700.11248817219954078No Hit
GCACAAGGGATACATGTGCTGCCCTTTTGTTCTGGACAAAGATGGAGTCAGT1700.11248817219954078No Hit
TTATCAGGGGATTGGTTGCTTTTTGCAACCAATGACCTGCATTCTGAGGTGT1700.11248817219954078No Hit
GTCGCCGGGAGATGCTGTAATTTACAGACATTTTCTATTTCCTCTGATAATA1700.11248817219954078No Hit
AACTTGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACA1690.11182647706895524No Hit
TCTGATGGGATTCTAGTTTTTGTTAATTTCTGTGTTTGCATTTTTTTCGGTG1690.11182647706895524No Hit
TTGCCTATGTTTTTAAGATAAAATCTCCCTCCCCTTCGAAAATCAAATTAGG1690.11182647706895524No Hit
TATCAGGGGGGATTCATTTGCAGTCCCCTAAATATCTTTTTTGATGCCATTT1690.11182647706895524No Hit
TGATGAGGGTATGGAAATCACTGTGTCAATCTGAAGGTGTGAGCCTTCGGTG1680.1111647819383697No Hit
CTAAAGGGATCAGTGTATGTTTTTTGTATTATCAAAAAAACCTTTTGAGTAG1670.11050308680778417No Hit
TTCTGCGGGGCACAGGTTTATATCAGCGCAAGCCTTCTAATGCAACACCGAC1670.11050308680778417No Hit
CACAATGGGAGGATATTCTGCACATTATGGAAAAAGGTAAATTTTAGAAGTT1670.11050308680778417No Hit
TGTATAGGGAAGGAATCTTCAGAAACTCATAGCATCTCCATGTTGCAGATAA1670.11050308680778417No Hit
TGTTGCCGGGAAGAGAGTGATTATTTGCATTACAAAGGAATTCTTAATAATT1670.11050308680778417No Hit
TAAACGGGGAAAATAGTCACCAGGCACTTCTACAAGATCCTAATGCTGCTCA1670.11050308680778417No Hit
GCTCATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1660.10984139167719865No Hit
GGTACTGGGGAAACAGATGGACAAAAGGTAACAGATAAGTAGATCTGAGGAA1660.10984139167719865No Hit
ATTTCCGGGGCAGAAAATATCCATCAATCTGTCTGAGTGGTCCCTGTAAAAT1660.10984139167719865No Hit
TTCCCTGGGGTGCTGGTATATTTGCCTATAAATCATCATGCACATAGAAAAC1660.10984139167719865No Hit
CAATATGGGTGTTAGATAAGCATATGTTACTTACCTTGTTATTAAATATTTC1660.10984139167719865No Hit
GTAGTGGGGAACCAACCCCTACTCTTTAATGGGCCAGACATGTGCTTTTAGT1650.10917969654661312No Hit
TATGTGGGGCAAATTAGAAGAAAGTGCCAGAGAACACCACATACCTTGTCCG1650.10917969654661312No Hit
GTACGAGGGACAACAAAGGTGAAGAAGTGAAGAGGATAGAATTCAAGCATGG1650.10917969654661312No Hit
TAGCAAGGGATTCCTTTTAGCATTACTATTTTTTGTTAAGAATTTGGACAAA1650.10917969654661312No Hit
CTGCTAGGGCATTGGGGAAATGAAAGACTTTTAAGTAAAACGATTTTGAGTG1650.10917969654661312No Hit
CTGAACTGGGAAGTCGTGCAGGAACCCATATGTTAAGAAAATGAGCTTTGTA1640.10851800141602759No Hit
CTATACGGGGTCTTAGTTCATTTTGTGTTGCTATAACAGAATACCACAGACT1640.10851800141602759No Hit
AAGCCTGGGCAACGGAGCGGGACTCTGTCTCAAATAAATAAATAAATAATAT1630.10785630628544204No Hit
GGCAGGTGGGAAACTGAAGGAATATAAAATGTTTGTCTGTTAAACAATACTT1630.10785630628544204No Hit
ACTCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1630.10785630628544204No Hit
GTCTGAGGACCCATAAATCTTCTTGCTGCCTTTGGCTCACCACCATCCCCTC1620.10719461115485651No Hit
CATTTAGGGAATGAATGCACAGTACACATAAAATTAATTGAACTCCATCAAT1620.10719461115485651No Hit
AGAGGGGGGTACTTAAGTGACTTTTATCCATTTTTTTCTAACATTATGAACA1620.10719461115485651No Hit
TAAAACGGGGACCCTCCCCAGTGCACTGAAGAAGGTAACCCGGTCCAGACCC1610.10653291602427098No Hit
CAGCTTGGGTCCAGAAGCTATTGTCTATTCAATTCAAAATCTACCTGAAGAA1610.10653291602427098No Hit
ATGCGGGGGAACTTATGTCAGTTACTGATATCCAGTTATGTCTGCTCATACT1610.10653291602427098No Hit
CCAGCCTGGGCAACAAGAGCGAAACTCCGTCTCAAAATTAAACAACAACAAC1600.10587122089368545No Hit
TTGCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10587122089368545No Hit
AATGAAGGGAACAAAAGAAATCACAAAATTAAATGTACTACAAGTTGAATAT1600.10587122089368545No Hit
ACATTTGGGAGCAGAGGAAACGGCTGCAAGAGCTGGATGCTGCATCTAAGGT1600.10587122089368545No Hit
AAAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10587122089368545No Hit
AGAAACGGGCCACAGCCCTTCTGAATTTCTCACAAGGTAATCTACCTAACAG1600.10587122089368545No Hit
TGCCACGGGTACCTGCAGTGAACATTTGGAATTTGAAATTAAAAACATAATA1600.10587122089368545No Hit
CTAAAAGGGGTCACGGCCATCAGCTTACACAACATCAGAAAACGCACAGTGG1590.1052095257630999No Hit
GAAGCAGGAACCCAGGCTCCAGAAGGTGTTGCCAGGGGCCCAGATGCCCTGA1590.1052095257630999No Hit
TTTGCTGGGAACTTCTTTGACTCTGAAATAATTAGATGCAGAGAATCTTTGT1590.1052095257630999No Hit
CCGTTAGGGCTGTGTGGAATATGGCATAATTGTAATAAATTATTTAGGAAAG1580.10454783063251437No Hit
TCAGCGGGGGACTCTACTTCAGAGGAAGTTGCTCTCTGGGAGAAGATGGTAA1580.10454783063251437No Hit
ATTCTTGGGGGAATTTAAATAATTCCTATTTGCAAATTAGAATGACAGTGTT1580.10454783063251437No Hit
TGAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.10454783063251437No Hit
CCCCGAGGGCTACACGACAGGCTTTTTTCCTCTAGAAAAATTCTGGTATTCA1580.10454783063251437No Hit
AGACCCGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1580.10454783063251437No Hit
TTAGTCGGGGCAAAACGCCATCTCTAAAAAAAATTTTTTTTAAATAAATAAC1580.10454783063251437No Hit
ATGCAGGGGTAGGAGGGGTTAATAAGTCTCTAGCTCTCCATCTATTGATAGT1570.10388613550192884No Hit
TTATAGGGACTCATTGAAGCACAAAAATATCCGCTACACAGGTTCACATCAG1570.10388613550192884No Hit
TAAGCTGGGTATTTCTATTTCTAGATGCCTGTTACAAAACATAGAAATTATC1570.10388613550192884No Hit
ACTTGTGGGGATCAGAGTTGAGTCATGTCTCAACATTAGATATCTTACTAGG1570.10388613550192884No Hit
TCTCCGGGTACGTTGCCCTCTGTGCTATCAATCATCTTATCATCAATTATTA1570.10388613550192884No Hit
GTGCCTGGGGGAGACAGATGCGAGGCGGCGGTCAGGTGGGATGCTATGGAAT1570.10388613550192884No Hit
GAGGCTGGGAATTTGGGATAAATGTTCAAAGAAACTTTGCTTTAAAGAGAAT1560.1032244403713433No Hit
GCAAAAGGGATATTTAATTTACTTTTTGTTACTCTTTACATACTGTTTATTT1560.1032244403713433No Hit
GTCGCTGGGATAAAGAGAAGATTTGAATTTTCAGTTTCATTTTCAAAAACTA1560.1032244403713433No Hit
ATAGCTGGGGGAGGGCTCTCCCCGAGTTCTTCAACGGCAAGAACAAGTCCAA1560.1032244403713433No Hit
AGGAAGGGAACTGTATGTACAATAACCTGCTTGAAGTTGTCCTGTAGCTCAC1550.10256274524075776No Hit
GTGTCCGGGTCGCCTTTGATTACCGAAAGCGCAAACATGTCTTCAAGCTGGG1550.10256274524075776No Hit
GGTAATGGGTGAAGACGCTGTTTGAGCAAGCCAAGCATGCCAGCACCTACCG1550.10256274524075776No Hit
ACTTGTGGGGGTCCTCAGCCCGCGCGCCGCCATCGCCGTCATGCTGGGCGCC1550.10256274524075776No Hit
GACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.10190105011017225No Hit
TAAGACGGGATATGTACTCTGACCTTAACAGTAATAAAGTAACCTCACAACG1540.10190105011017225No Hit
AATGTTGGGCAATATTTCCCAAATTTCTATGCGCTACTGAAAAGTATTGATT1540.10190105011017225No Hit
TGACAGGGAGGAGATATTTTGGGTTCAAAAAAAGTAAAAAGATAATATAGCT1540.10190105011017225No Hit
TTCTGCGGGGTAACTAAATTGAGTTCAAATTCTATCCCAGCTTAGACTGGTT1540.10190105011017225No Hit
GTTCCGGGTATTACAGTGGAACTGAAGAAGGAGGAGGAATGTGAGAGCTACA1530.10123935497958672No Hit
CTCTCAGGGCAGAGAAAAAGTTCTAACATAAAGGTGAATGACCTTGACCGTT1530.10123935497958672No Hit
TTGGCTGGGTGGACAAATTTCTTAAAAGATAAAAATTCTTAAAACTGACACA1530.10123935497958672No Hit
CGGGCAGGGTGTTAAAAGTAAGCTTTCACAATCAGCATTTAGCATTTGTTGA1520.10057765984900117No Hit
CATAGGGGTATTGTTTAATTTCTTCAAATTTGTGACTATTGCAGTTTTACAT1520.10057765984900117No Hit
CTCGCGGGGAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACA1520.10057765984900117No Hit
ATCACGGGATGTGATAATGTGGAGACAAAGCAAGATTTGATTACATAAACTT1520.10057765984900117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)