FastQCFastQC Report
Sat 31 Dec 2022
SRR5844141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149873
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5720.38165646914387513No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT4890.32627624722264853TruSeq Adapter, Index 2 (95% over 22bp)
GAGTTGGGTGAGAAGCAGCTTCCATCTTCAACCTCGGATGATCGGGTAAAAG2510.16747512894250466No Hit
CACATAGGGATTCACACACAAACATGACAGAAAAGTCATGAAGACAATAATT2370.15813388669073147No Hit
ATGCTTGGGAAATGTGCATATGTCACAAGAATTCTGTGAATATATTAAATCA2250.15012710761778306No Hit
CCAACCTGGGCGACAGAGCGAGACTCTGTCTCCAAAACAATAAATAAATAGA2250.15012710761778306No Hit
AAGCCTGGGCAACGGAGCGGGACTCTGTCTCAAATAAATAAATAAATAATAT2240.1494598760283707No Hit
TGAAGGGATCCTCCACATGGGAGGTAAATTGTTGAAACCTTGCATGACTGAA2200.1467909496707212No Hit
AGACCAGGCTATGAAGTGTTCTTGGAAACTAGGATGGAAGAAATAGGCCACA2200.1467909496707212No Hit
CTAATAGGGCTACCCCTTCACTATTTCTTTATTTCTAACTCTTTTCCATTGT2160.14412202331307175No Hit
AGCGATGGGGGAAGAACGAGAGGGGGAGAGATGTAAGAAAAGAAAGGAAAAG2150.14345479172365938No Hit
CTACTTGAGGGGATTTACACCTGAAACTAAATACAGAATGTTCTATCTCTAC2150.14345479172365938No Hit
CGCTAAGGGGACCCTACTGTAATAGGATTCTCTGATTTCTATATTTCAGAAT2120.14145309695542224No Hit
AATTTTGGGGATCCCACATCTCTTTCAAAAGGTGATAATTAGAGTGAAGTTA2120.14145309695542224No Hit
GCGAGTGGGTCCGATCTTTTGCCGGGAATGATAAGAGCAAAATTTGTGGAGT2120.14145309695542224No Hit
GGAGCCGGGCAAGTCACTAACCTTCCTCTTTGATCTCTTTGTAGGCTGACCA2110.14078586536600987No Hit
TTAGTCGGGGACGCTCTTCCGATCTTCGATGGGGTCCTGTGGCAGTGGCGAT2110.14078586536600987No Hit
CTTGAGGGGGGGTTTTCCTGTAAGTCGTATTAATAATTCTCTAAGGGGACCC2100.1401186337765975No Hit
CGTTAAGGGCTAGTTGGTGTGCAGTGGTATCTTGTTGTTTTAATTAGCATTT2060.13744970741894805No Hit
CGATCGGGGGACGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCCATGGAG2050.13678247582953568No Hit
AGCTATGGGCGATCTTGTTCGGGGGACCAAATTGCAGACTAATTGTTTCCAT2050.13678247582953568No Hit
TCATCGGGGTTTTATAAAGACTTCATTGATAAGATGGCTTTTATTCATACTA2040.1361152442401233No Hit
AGCTGTGGGCACACTCCAAAATTGAGCTATAGAGAGAAACAATTTTGGAGCT2030.13544801265071094No Hit
TGCACAGAGGACAGAAACTATAAAAGTAAGCAAACGAAAATGGAAATAATGA2020.13478078106129857No Hit
TACAGTGGGAGGCTGTATCTTCTGCCACTGTAATAAAATAGATGCAATTGAG1990.13277908629306145No Hit
ATCAGCGGGTGTCCCTGCACAGCCCATCACCACCACCTTTTTGGAGAGACAT1980.13211185470364908No Hit
TCTGATGGGTCCTCTCTTCTGTAGGAGCTTTGGGTTTTCAGAGGTGCAGGGA1970.1314446231142367No Hit
AAATAGGGGTTCATGGAATCTTCAAGGAAAACCAAATATTCTTTTTTAATAA1960.13077739152482434No Hit
TTGCTAGGGAGTCTTCCTCGATCCCGTGGTGCTCCGCGGCGCGGCCTTGCTC1960.13077739152482434No Hit
CTCCACGGGGGTTTCTGCGGCGGCTGGAGAGGTGGTCGGAGAAGTAGGAACC1960.13077739152482434No Hit
GAAAATGGGCTACTTAAGTGGAAAGTGACTGAAAGATCTGGAAAAGTGGCTT1960.13077739152482434No Hit
TGTCACGGGGCAGAGTTCAGACTTAGGAGTAAAACAGAAAAGTGAACTAAAC1950.13011015993541197No Hit
TCTCCGGGTACGTTGCCCTCTGTGCTATCAATCATCTTATCATCAATTATTA1950.13011015993541197No Hit
TGCGTTTGGGGTTCCTTCTTGTAAGCACACACGTCAGGTTGAACTCTGACCT1940.1294429283459996No Hit
GCTGCTGGGCAGAGGAAGTTTGTTTTTTAATTTAAGAGGGAAATATAACCTA1940.1294429283459996No Hit
GCGCGGGGCAGGGACTAACAATGTTAATCTGATAAGGACAGCAAAATCATCA1940.1294429283459996No Hit
AGAATGGGGGGTTAATTCCCATTGGTGTCTTTGCTTATAGCATTTTTTCTCT1940.1294429283459996No Hit
TGACATTGGGAGAAGAGTATTAATATGAAATAATGAATAAGGACATTAAAAG1920.12810846516717486No Hit
ACTTATGGGCTGCTGTAAATATACTTAATAAGAAGGCTAACTAGGCAACCTA1920.12810846516717486No Hit
ACTACTGGGACCAAGGATGCAGCTTGACTTTAAATCCATTCATGTTCAAGAA1910.1274412335777625No Hit
ACAGCGGGGGCTGAAAAATAGGACCCAACTATATGCTACCTACAAGAGATTC1910.1274412335777625No Hit
AGTTGCGGGCTATCAGGGCGCTGTCAGACTGGCTGCTGGAGCCCAGATGGCA1910.1274412335777625No Hit
TGACAGGGAGGAGATATTTTGGGTTCAAAAAAAGTAAAAAGATAATATAGCT1900.12677400198835015No Hit
TTTAACGGGCACTGAAGTGGCATCCAGATAAAAATCCTGAGAATAAAGAAGA1900.12677400198835015No Hit
CTGCTAGGGCATTGGGGAAATGAAAGACTTTTAAGTAAAACGATTTTGAGTG1880.1254395388095254No Hit
TTTGTTGGGGAGTAACAAGTTAGTGACTGGGTTAGGGGGTGGTTAGGACAGT1880.1254395388095254No Hit
TCGCTGGGGGATCCACTTTTAATTCCTGAGGTTCCATGAGGAAAACAGAGGT1880.1254395388095254No Hit
TGTATAGGGTCTCGTGTCATGTATTACATAGCAGGTTTTTGGCAGAGACTGC1860.12410507563070067No Hit
AAGATTGGGACTTCAAGCAGTATTTCTATCATTTTACTTATTCCAAGTCAAA1840.12277061245187593No Hit
AGCTCGGGGATCCTTGCTGGGGACTGAAAACGCTTCTGTGAGACTTGATAAT1840.12277061245187593No Hit
TAAGCACGGGTTCATTTTAAATCATTGTTTGCTAGATACTGCTAAATATCAT1840.12277061245187593No Hit
GCGTATGGGGAACTATTTCATAATGCTATGCTTTCTATGGCAAAGAAAATAG1840.12277061245187593No Hit
TTATGTGGGTGATACTGGATTACATATGAGCAAGAAGATGTCCAGTATGACT1830.12210338086246356No Hit
TAGCAAGGGATTCCTTTTAGCATTACTATTTTTTGTTAAGAATTTGGACAAA1830.12210338086246356No Hit
TTTGCCCGGGTATGTAGAATTTTCATGGTTATTTTGTATGAATTATTTTTCT1830.12210338086246356No Hit
GAAATTGGGTGCCCACTTGCCCAGGTCTAATAAACACTAAAGGGGGGAAATT1820.12143614927305117No Hit
GTTTATGGGTTTTCAACTACTTTCTCTACAGCTTTTCATGTAAATTAGTCTT1810.1207689176836388No Hit
TATGTTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1810.1207689176836388No Hit
CTTTAAGGGGGCGTAAGTAAGTGAGTCTAGCAGAAAGCTCTATTTCATCAAA1800.12010168609422643No Hit
CATGGGGTGGAGCCCCGGGGAGGGCAAGGCGACGGCCTCGCCCTTGTGCTGA1800.12010168609422643No Hit
TCTTCTGGGATCCGGCAGAGGGAGGAAGGCAGAGCAAGTGGAGCAGGGCTTA1800.12010168609422643No Hit
GCGCGAGGGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTATGGTAGCTT1790.11943445450481407No Hit
GTCGCCGGGAGATGCTGTAATTTACAGACATTTTCTATTTCCTCTGATAATA1780.1187672229154017No Hit
ATACAAGGGGTGCTAGTTACTCTAAGAAAAACACCAAGTCTCAGTGGCTTCA1770.11809999132598933No Hit
TTAGTTGGGGGATTTGAAAATGTATATGGCCTAGGAATTATTGAAATTATTT1760.11743275973657696No Hit
AGAGGGGGGTACTTAAGTGACTTTTATCCATTTTTTTCTAACATTATGAACA1760.11743275973657696No Hit
ATTTAGGGGATCACTAGGCAATTCTGCTTTCTAAGCAGTTGTATTGAAAACA1760.11743275973657696No Hit
GGCGAAGGGATAGCGAATGCACCTGTGCGTTGAAGGCTGTTTCGCTGTAACA1750.1167655281471646No Hit
ACACGCGGGCAAATAAATGGAACCAGAAATTGGAAAACTTACAGAAAGCAAA1750.1167655281471646No Hit
AAAAAAGGGGCTTTATTAGGAAAAAAAATCAGCTCCAGAATACTCTGCTAAC1750.1167655281471646No Hit
TGCGAGGGATGGCTTATGAGAGAAGCATTTCTTAAATCTGGAAAACAAAACA1740.11609829655775224No Hit
TTGCCTATGTTTTTAAGATAAAATCTCCCTCCCCTTCGAAAATCAAATTAGG1740.11609829655775224No Hit
GTTTGCGGGTGGCTTGGCTTCCCTGACTAAAGAATTAAGTCTCATTTTTACT1720.1147638333789275No Hit
CTAAAAGGGGTCACGGCCATCAGCTTACACAACATCAGAAAACGCACAGTGG1720.1147638333789275No Hit
ACTGGAGGGGATACATTCAGATCGGTAATAGCATGTTGAAAAAGAATGTGTG1720.1147638333789275No Hit
AAATATGGGGAGGGTTAGTTGGGGAAAAACTTCATTCTCAGGAAAAGACTTG1710.11409660178951513No Hit
AAAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1710.11409660178951513No Hit
CAGCTTGGGTCCAGAAGCTATTGTCTATTCAATTCAAAATCTACCTGAAGAA1700.11342937020010276No Hit
ATCGGGGGACTGAGGCCAGCTCCTCTGATGCCCCCAGGGATTCAGTCTGGCT1700.11342937020010276No Hit
GAAGCAGGAACCCAGGCTCCAGAAGGTGTTGCCAGGGGCCCAGATGCCCTGA1700.11342937020010276No Hit
GGGTCCGGGTGAGACTGACATATGCCATTATCTCTTTTCCAGTATTAAACAC1700.11342937020010276No Hit
TCTGATGGGATTCTAGTTTTTGTTAATTTCTGTGTTTGCATTTTTTTCGGTG1700.11342937020010276No Hit
ATACCCGGGGATTGGCTCCAGGGAGGCATGATGCCACCGGGCAGCTCTTTTT1700.11342937020010276No Hit
CCGTTAGGGCTGTGTGGAATATGGCATAATTGTAATAAATTATTTAGGAAAG1690.1127621386106904No Hit
CTAAAGGGATCAGTGTATGTTTTTTGTATTATCAAAAAAACCTTTTGAGTAG1690.1127621386106904No Hit
CACCACGGGCGATCTGATTTGGGGGCCATGTTTCTGTCCCTGGTGTTCCTGG1690.1127621386106904No Hit
ACACTAGGGGAGATGAAAACTGAAGAAAAATGCCAATGTGACGTTTGTGTAT1690.1127621386106904No Hit
CTGTTAGGGAACTGTTCCCATTTAGAGCCACCAGTTTCCAGGCTTGAAGGTG1680.11209490702127803No Hit
CTGAACTGGGAAGTCGTGCAGGAACCCATATGTTAAGAAAATGAGCTTTGTA1680.11209490702127803No Hit
AGGAAGGGAACTGTATGTACAATAACCTGCTTGAAGTTGTCCTGTAGCTCAC1660.11076044384245326No Hit
GGTACTGGGGAAACAGATGGACAAAAGGTAACAGATAAGTAGATCTGAGGAA1660.11076044384245326No Hit
AGCTCAGGGAAGACTTCAGAGGCCGACTGGCTCTGGAATCTTCTGCAGGGCA1660.11076044384245326No Hit
CAATATGGGTGTTAGATAAGCATATGTTACTTACCTTGTTATTAAATATTTC1660.11076044384245326No Hit
TGCCTTGGGGAGAGAGAGAATGAAGTTGACTTCAAAAATTTGGTTGGTTGTT1650.1100932122530409No Hit
AATCTCGGGGTAGACTTATTTTATTTTTTAATGCATAGGATCTTTTGCCAGA1650.1100932122530409No Hit
ATGCAGGGGTAGGAGGGGTTAATAAGTCTCTAGCTCTCCATCTATTGATAGT1650.1100932122530409No Hit
AAGCCGGGGATGGATTGAACTGTGTGCCCCTAAAAGATATGTTGAAGCCTGA1650.1100932122530409No Hit
GGAACGGGGATGGCAGAATACAGAAAATGCTTTATTGGAGACAGTCATTGTA1650.1100932122530409No Hit
GAGGCTGGGAATTTGGGATAAATGTTCAAAGAAACTTTGCTTTAAAGAGAAT1640.10942598066362853No Hit
TTGGCTGGGAGAGAATAATATACTTATTTTTAATGAAAACAAGAAAGTTATG1640.10942598066362853No Hit
AAGTTTGGGATTGTTGTTTATAATGACAAATTTTTGGGAAAATCCTACATCT1640.10942598066362853No Hit
ACTGCCGGGCAGGCTTACCTTTTAAAGTTTAGTATATAGAGACAATTTTAAT1630.10875874907421616No Hit
ACATTTGGGAGCAGAGGAAACGGCTGCAAGAGCTGGATGCTGCATCTAAGGT1630.10875874907421616No Hit
TAAGACGGGATATGTACTCTGACCTTAACAGTAATAAAGTAACCTCACAACG1630.10875874907421616No Hit
TCAATAGGGAATTCAAAAAGAGAAAGACGGCACAAGAGCAAGAGAACAGAAC1620.10809151748480379No Hit
GTCGCTGGGATAAAGAGAAGATTTGAATTTTCAGTTTCATTTTCAAAAACTA1620.10809151748480379No Hit
GAGGTTGGGGTGACGTTTTAGTCCCAGACTGAATTCCAAATTTGCTCTAGTT1620.10809151748480379No Hit
TCTCGAATTGGGCGATCTTATCGCGGGAAATGAATTTGAGTTATCAAAAAAC1620.10809151748480379No Hit
GTAGAGGGGGAACCTGGATTGTGGTTTGATTTTTTTCATTTTCAACCTATAT1620.10809151748480379No Hit
TTACCTGGGGTGAGTTAATACTTTAGAAAAAAATCCATCAACTCAGCCCCGT1610.10742428589539142No Hit
ACACGGGGGGGGCGCATGAAGGTGGGGGTGATGCCGAAGGGGAAGGGATCGC1610.10742428589539142No Hit
TATTGGGGAGGGCTGTAAAATGCATGATTTTGTAACCCAGATTTTGCTGTAT1610.10742428589539142No Hit
ATTTCCGGGGCAGAAAATATCCATCAATCTGTCTGAGTGGTCCCTGTAAAAT1610.10742428589539142No Hit
GGTAATGGGTGAAGACGCTGTTTGAGCAAGCCAAGCATGCCAGCACCTACCG1600.10675705430597907No Hit
CATAATGGGAGTTCTTAGAGGAAGGAACATTAAGAGTAACAATCACAATGCC1600.10675705430597907No Hit
CATTTAGGGAATGAATGCACAGTACACATAAAATTAATTGAACTCCATCAAT1590.1060898227165667No Hit
GACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.10542259112715432No Hit
TGTATAGGGAAGGAATCTTCAGAAACTCATAGCATCTCCATGTTGCAGATAA1580.10542259112715432No Hit
TTATCAGGGGATTGGTTGCTTTTTGCAACCAATGACCTGCATTCTGAGGTGT1580.10542259112715432No Hit
TACCCAGGGGAGATTTTAATGAGTTATCACTTTTTCAGCTGATATATTCATT1570.10475535953774195No Hit
ACTAGGGATATGTGCATTAGTCAACCTATTGCGAAACCATCTGACAAACAGA1560.10408812794832958No Hit
CTACGGGTGTTTTGAGAATCCTCTCCATTTTCCCAAATAAAAACCTATCACA1560.10408812794832958No Hit
TTCTATGGGTACTTGAATTTTCATGTCAGGGGCCCTTCATATAACATCACTA1560.10408812794832958No Hit
CATGTTGGGTCTCAGATGAGAGGCTTGAATTTGATGGCCAGATATAACCTCT1560.10408812794832958No Hit
TCCTAGGGGTTTTGAAATAAATGAAATGTTGAAAATTTTGCTAGTTGGTTAG1560.10408812794832958No Hit
TAAACGGGGAAAATAGTCACCAGGCACTTCTACAAGATCCTAATGCTGCTCA1560.10408812794832958No Hit
TTTGCTGGGAACTTCTTTGACTCTGAAATAATTAGATGCAGAGAATCTTTGT1550.10342089635891723No Hit
CTGCTGGGGATGGTGAGTTTTATTTGATGTAAAATATGATTTTTGCTTTTTA1550.10342089635891723No Hit
AGAAACGGGCCACAGCCCTTCTGAATTTCTCACAAGGTAATCTACCTAACAG1550.10342089635891723No Hit
GCAAAAGGGATATTTAATTTACTTTTTGTTACTCTTTACATACTGTTTATTT1540.10275366476950486No Hit
ACTCAAGGGGCCTAGGATTCGTTGCTGTGAGGACTAATGACAAATTGCATGT1540.10275366476950486No Hit
CGCTCAGGGCCAGGAGAGATGTTTTTGCAGCATGTATGCTAAATTTGTGAGT1540.10275366476950486No Hit
AATGTTGGGCAATATTTCCCAAATTTCTATGCGCTACTGAAAAGTATTGATT1540.10275366476950486No Hit
ATTAAAGGGAATGTGGCTGATGAAGGCAACTTGGCCTTCATACCTAACCTTT1540.10275366476950486No Hit
TAAGCTGGGTATTTCTATTTCTAGATGCCTGTTACAAAACATAGAAATTATC1540.10275366476950486No Hit
CGCGCCGCGTGCAGCTCATCCTGGACGACCTGGGCGTGGACGCGGCCGAGGG1540.10275366476950486No Hit
TGATGAGGGTATGGAAATCACTGTGTCAATCTGAAGGTGTGAGCCTTCGGTG1530.10208643318009249No Hit
TCACTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1530.10208643318009249No Hit
AACACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1530.10208643318009249No Hit
GTACGAGGGACAACAAAGGTGAAGAAGTGAAGAGGATAGAATTCAAGCATGG1530.10208643318009249No Hit
ACTTCAGGGCAGACCCCAGGCTTAAGTGATGATGAGTTGGCCAGCTTGGTTA1530.10208643318009249No Hit
GTAATAGGGTTGATCCAGTATTTCAACACAAATTTACTTTGCTCATAAAATG1530.10208643318009249No Hit
CCCCGAGGGCTACACGACAGGCTTTTTTCCTCTAGAAAAATTCTGGTATTCA1530.10208643318009249No Hit
AACTTGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACA1520.10141920159068012No Hit
GTTTGGGCCCTCTTGACTAGTGTAGCATGTGGTATGTTTAAAATATTTCATT1520.10141920159068012No Hit
CGGGCAGGGTGTTAAAAGTAAGCTTTCACAATCAGCATTTAGCATTTGTTGA1520.10141920159068012No Hit
ACTCCCGGGTATTAGAGCAATTCATATAGAGATATGAAAATCGTTATTAGAT1520.10141920159068012No Hit
ACTGCTGGGGATCCTTCCATTTCATTTATTCATCCATCCATTCAACAAGTAT1520.10141920159068012No Hit
GACCGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1510.10075197000126775No Hit
TCAGCGGGGGACTCTACTTCAGAGGAAGTTGCTCTCTGGGAGAAGATGGTAA1510.10075197000126775No Hit
AGTATAGGGGAGAATATTTTGGTGGAAAGCTATTTCAGTGAAGCCAGACTAT1510.10075197000126775No Hit
TAACAGTGGGGATGCATAATAAATGCTGAAAGTAAAAAGCCTGAATTATTTA1510.10075197000126775No Hit
GTGCCTGGGGGAGACAGATGCGAGGCGGCGGTCAGGTGGGATGCTATGGAAT1510.10075197000126775No Hit
CCAGCCTGGGCAACAAGAGCGAAACTCCGTCTCAAAATTAAACAACAACAAC1500.10008473841185536No Hit
AAGCTTGGGTATTGCTGATGTTCATTTGGTCACCTGGCTGATGTTTAACAGG1500.10008473841185536No Hit
GACTCTGGGTACATAAAGTACTCTAGTTTGAACCAGAAGTTTTATTAAACTA1500.10008473841185536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)