FastQCFastQC Report
Sat 31 Dec 2022
SRR5844140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149523
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5910.3952569169960474No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT4860.3250336068698461TruSeq Adapter, Index 2 (95% over 22bp)
AGACCAGGCTATGAAGTGTTCTTGGAAACTAGGATGGAAGAAATAGGCCACA2310.15449128227764292No Hit
TTAGTCGGGGACGCTCTTCCGATCTTCGATGGGGTCCTGTGGCAGTGGCGAT2270.15181610855854952No Hit
GAGTTGGGTGAGAAGCAGCTTCCATCTTCAACCTCGGATGATCGGGTAAAAG2240.14980972826922948No Hit
AGAATGGGGGGTTAATTCCCATTGGTGTCTTTGCTTATAGCATTTTTTCTCT2230.14914093483945615No Hit
CACATAGGGATTCACACACAAACATGACAGAAAAGTCATGAAGACAATAATT2210.14780334797990943No Hit
AAGATTGGGACTTCAAGCAGTATTTCTATCATTTTACTTATTCCAAGTCAAA2130.1424530005417227No Hit
CGATCGGGGGACGCCGGAAGTGGTTCTCCGCCCCTGCCACTGGGCCATGGAG2130.1424530005417227No Hit
GCTGCTGGGCAGAGGAAGTTTGTTTTTTAATTTAAGAGGGAAATATAACCTA2110.14111541368217598No Hit
TCAATAGGGAATTCAAAAAGAGAAAGACGGCACAAGAGCAAGAGAACAGAAC2100.14044662025240265No Hit
TCGCTGGGGGATCCACTTTTAATTCCTGAGGTTCCATGAGGAAAACAGAGGT2100.14044662025240265No Hit
ACTGGAGGGGATACATTCAGATCGGTAATAGCATGTTGAAAAAGAATGTGTG2090.1397778268226293No Hit
TCATCGGGGTTTTATAAAGACTTCATTGATAAGATGGCTTTTATTCATACTA2070.1384402399630826No Hit
CTAATAGGGCTACCCCTTCACTATTTCTTTATTTCTAACTCTTTTCCATTGT2070.1384402399630826No Hit
TGCGTTTGGGGTTCCTTCTTGTAAGCACACACGTCAGGTTGAACTCTGACCT2050.13710265310353592No Hit
AGCTGTGGGCACACTCCAAAATTGAGCTATAGAGAGAAACAATTTTGGAGCT2010.13442747938444252No Hit
AGAGGGGGGTACTTAAGTGACTTTTATCCATTTTTTTCTAACATTATGAACA2000.1337586859546692No Hit
AAATAGGGGTTCATGGAATCTTCAAGGAAAACCAAATATTCTTTTTTAATAA1950.13041471880580247No Hit
CTACTTGAGGGGATTTACACCTGAAACTAAATACAGAATGTTCTATCTCTAC1950.13041471880580247No Hit
TGCACAGAGGACAGAAACTATAAAAGTAAGCAAACGAAAATGGAAATAATGA1940.1297459253760291No Hit
ACTTATGGGCTGCTGTAAATATACTTAATAAGAAGGCTAACTAGGCAACCTA1920.12840833851648242No Hit
TATGTTGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1920.12840833851648242No Hit
TGAAGGGATCCTCCACATGGGAGGTAAATTGTTGAAACCTTGCATGACTGAA1900.1270707516569357No Hit
GCGAGTGGGTCCGATCTTTTGCCGGGAATGATAAGAGCAAAATTTGTGGAGT1890.12640195822716238No Hit
GGAGCCGGGCAAGTCACTAACCTTCCTCTTTGATCTCTTTGTAGGCTGACCA1880.12573316479738902No Hit
ATGCAGGGGTAGGAGGGGTTAATAAGTCTCTAGCTCTCCATCTATTGATAGT1870.1250643713676157No Hit
TGTATAGGGTCTCGTGTCATGTATTACATAGCAGGTTTTTGGCAGAGACTGC1860.12439557793784235No Hit
TTGCTAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTGG1860.12439557793784235No Hit
AAGCCTGGGCAACGGAGCGGGACTCTGTCTCAAATAAATAAATAAATAATAT1850.123726784508069No Hit
TTACCTGGGGTGAGTTAATACTTTAGAAAAAAATCCATCAACTCAGCCCCGT1850.123726784508069No Hit
AGCGATGGGGGAAGAACGAGAGGGGGAGAGATGTAAGAAAAGAAAGGAAAAG1850.123726784508069No Hit
TGAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1840.12305799107829565No Hit
TTATCAGGGGATTGGTTGCTTTTTGCAACCAATGACCTGCATTCTGAGGTGT1840.12305799107829565No Hit
AATTTTGGGGATCCCACATCTCTTTCAAAAGGTGATAATTAGAGTGAAGTTA1830.1223891976485223No Hit
CTGTTAGGGAACTGTTCCCATTTAGAGCCACCAGTTTCCAGGCTTGAAGGTG1830.1223891976485223No Hit
CCAACCTGGGCGACAGAGCGAGACTCTGTCTCCAAAACAATAAATAAATAGA1830.1223891976485223No Hit
GCGCGGGGCAGGGACTAACAATGTTAATCTGATAAGGACAGCAAAATCATCA1810.12105161078897561No Hit
GAGGTTGGGGTGACGTTTTAGTCCCAGACTGAATTCCAAATTTGCTCTAGTT1810.12105161078897561No Hit
TTTGTTGGGGAGTAACAAGTTAGTGACTGGGTTAGGGGGTGGTTAGGACAGT1810.12105161078897561No Hit
TTTAACGGGCACTGAAGTGGCATCCAGATAAAAATCCTGAGAATAAAGAAGA1810.12105161078897561No Hit
GGGTCCGGGTGAGACTGACATATGCCATTATCTCTTTTCCAGTATTAAACAC1800.12038281735920225No Hit
TCTCCGGGTACGTTGCCCTCTGTGCTATCAATCATCTTATCATCAATTATTA1800.12038281735920225No Hit
GCGCGAGGGAACTTTTTACAAAGAAAAATGGAAAAACTTTGTATGGTAGCTT1790.11971402392942893No Hit
AGCTCGGGGATCCTTGCTGGGGACTGAAAACGCTTCTGTGAGACTTGATAAT1790.11971402392942893No Hit
CTCCACGGGGGTTTCTGCGGCGGCTGGAGAGGTGGTCGGAGAAGTAGGAACC1790.11971402392942893No Hit
ATGCTTGGGAAATGTGCATATGTCACAAGAATTCTGTGAATATATTAAATCA1780.11904523049965557No Hit
CTTTAAGGGGGCGTAAGTAAGTGAGTCTAGCAGAAAGCTCTATTTCATCAAA1780.11904523049965557No Hit
TAAGACGGGATATGTACTCTGACCTTAACAGTAATAAAGTAACCTCACAACG1780.11904523049965557No Hit
CTAAAGCCGGGGTGGAATGGTTTATTTTATGGAAGAGAATTCATTGGAGGTG1780.11904523049965557No Hit
ACTTGTGGGGGTCCTCAGCCCGCGCGCCGCCATCGCCGTCATGCTGGGCGCC1780.11904523049965557No Hit
TTGGCTGGGAGAGAATAATATACTTATTTTTAATGAAAACAAGAAAGTTATG1770.11837643706988223No Hit
AAGTTTGGGATTGTTGTTTATAATGACAAATTTTTGGGAAAATCCTACATCT1770.11837643706988223No Hit
TGCCTTGGGGAGAGAGAGAATGAAGTTGACTTCAAAAATTTGGTTGGTTGTT1760.11770764364010888No Hit
GCGTATGGGGAACTATTTCATAATGCTATGCTTTCTATGGCAAAGAAAATAG1760.11770764364010888No Hit
ATCACGGGATGTGATAATGTGGAGACAAAGCAAGATTTGATTACATAAACTT1760.11770764364010888No Hit
GGATCAGGGGTTGTTTATCATATAATAATTTGTTTTGTCATATTTGCTTTCA1750.11703885021033553No Hit
ACACGCGGGCAAATAAATGGAACCAGAAATTGGAAAACTTACAGAAAGCAAA1750.11703885021033553No Hit
TAAGCACGGGTTCATTTTAAATCATTGTTTGCTAGATACTGCTAAATATCAT1750.11703885021033553No Hit
TGTATAGGGAAGGAATCTTCAGAAACTCATAGCATCTCCATGTTGCAGATAA1740.11637005678056218No Hit
CGACTAGGGTGAGAATGAAGGGATTTAAAACACTGATTTAACATTGAAAGGC1740.11637005678056218No Hit
CTGGATGGGTCCTGTTAGTGTTTATACTGAGCTAGTACTGTAACTTGCAAAT1730.11570126335078885No Hit
GACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1720.1150324699210155No Hit
TTAGTTGGGGGATTTGAAAATGTATATGGCCTAGGAATTATTGAAATTATTT1720.1150324699210155No Hit
ACTCCCGGGTATTAGAGCAATTCATATAGAGATATGAAAATCGTTATTAGAT1720.1150324699210155No Hit
AAGCCGGGGATGGATTGAACTGTGTGCCCCTAAAAGATATGTTGAAGCCTGA1720.1150324699210155No Hit
TACAGTGGGAGGCTGTATCTTCTGCCACTGTAATAAAATAGATGCAATTGAG1710.11436367649124216No Hit
ATCGGGGGACTGAGGCCAGCTCCTCTGATGCCCCCAGGGATTCAGTCTGGCT1710.11436367649124216No Hit
TAATAGGGGAGATATTTCCTTTTTCACCATAGGCCTCAAAGCGCTCCAAATG1710.11436367649124216No Hit
GTCGCTGGGATAAAGAGAAGATTTGAATTTTCAGTTTCATTTTCAAAAACTA1700.1136948830614688No Hit
GAAATTGGGTGCCCACTTGCCCAGGTCTAATAAACACTAAAGGGGGGAAATT1700.1136948830614688No Hit
TGACAGGGAGGAGATATTTTGGGTTCAAAAAAAGTAAAAAGATAATATAGCT1700.1136948830614688No Hit
GTCGCCGGGAGATGCTGTAATTTACAGACATTTTCTATTTCCTCTGATAATA1700.1136948830614688No Hit
GCAAAAGGGATATTTAATTTACTTTTTGTTACTCTTTACATACTGTTTATTT1690.11302608963169546No Hit
ACAACAAGGGAACATCATGTGGGACAAAAGAATCTGAAGAACAGCCTTCATC1690.11302608963169546No Hit
ACTCAAGGGGCCTAGGATTCGTTGCTGTGAGGACTAATGACAAATTGCATGT1690.11302608963169546No Hit
TTCTGCGGGGCACAGGTTTATATCAGCGCAAGCCTTCTAATGCAACACCGAC1690.11302608963169546No Hit
CAACTGGGTGGTTATTGCCAATGGCTAAAAATAAAATTCAAACTTCTTCCTA1690.11302608963169546No Hit
ATTAAAGGGAATGTGGCTGATGAAGGCAACTTGGCCTTCATACCTAACCTTT1690.11302608963169546No Hit
CATAATGGGAGTTCTTAGAGGAAGGAACATTAAGAGTAACAATCACAATGCC1690.11302608963169546No Hit
GTTTGCGGGTGGCTTGGCTTCCCTGACTAAAGAATTAAGTCTCATTTTTACT1680.11235729620192213No Hit
ACTAGGGATATGTGCATTAGTCAACCTATTGCGAAACCATCTGACAAACAGA1680.11235729620192213No Hit
ACACTAGGGGAGATGAAAACTGAAGAAAAATGCCAATGTGACGTTTGTGTAT1680.11235729620192213No Hit
TTAGTCGGGGCAAAACGCCATCTCTAAAAAAAATTTTTTTTAAATAAATAAC1680.11235729620192213No Hit
CCAGCCTGGGCAACAAGAGCGAAACTCCGTCTCAAAATTAAACAACAACAAC1670.11168850277214876No Hit
CGCTAAGGGGACCCTACTGTAATAGGATTCTCTGATTTCTATATTTCAGAAT1670.11168850277214876No Hit
AATGAAGGGAACAAAAGAAATCACAAAATTAAATGTACTACAAGTTGAATAT1670.11168850277214876No Hit
TATTGGGGAGGGCTGTAAAATGCATGATTTTGTAACCCAGATTTTGCTGTAT1670.11168850277214876No Hit
CGTTAAGGGCTAGTTGGTGTGCAGTGGTATCTTGTTGTTTTAATTAGCATTT1670.11168850277214876No Hit
CTCGCGGGGAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACA1670.11168850277214876No Hit
AAAAAAGGGGCTTTATTAGGAAAAAAAATCAGCTCCAGAATACTCTGCTAAC1670.11168850277214876No Hit
CTAAAGGGATCAGTGTATGTTTTTTGTATTATCAAAAAAACCTTTTGAGTAG1660.11101970934237543No Hit
TTTGCCCGGGTATGTAGAATTTTCATGGTTATTTTGTATGAATTATTTTTCT1660.11101970934237543No Hit
AACTTGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACA1640.10968212248282873No Hit
GTTTGGGCCCTCTTGACTAGTGTAGCATGTGGTATGTTTAAAATATTTCATT1640.10968212248282873No Hit
ATCAGCGGGTGTCCCTGCACAGCCCATCACCACCACCTTTTTGGAGAGACAT1640.10968212248282873No Hit
GCACAAGGGATACATGTGCTGCCCTTTTGTTCTGGACAAAGATGGAGTCAGT1640.10968212248282873No Hit
ATTTAGGGGATCACTAGGCAATTCTGCTTTCTAAGCAGTTGTATTGAAAACA1640.10968212248282873No Hit
TATCAGGGGGGATTCATTTGCAGTCCCCTAAATATCTTTTTTGATGCCATTT1640.10968212248282873No Hit
GTCTGAGGACCCATAAATCTTCTTGCTGCCTTTGGCTCACCACCATCCCCTC1630.10901332905305539No Hit
TTATGTGGGTGATACTGGATTACATATGAGCAAGAAGATGTCCAGTATGACT1630.10901332905305539No Hit
ACACGGGGGGGGCGCATGAAGGTGGGGGTGATGCCGAAGGGGAAGGGATCGC1630.10901332905305539No Hit
CATAGGGGTATTGTTTAATTTCTTCAAATTTGTGACTATTGCAGTTTTACAT1630.10901332905305539No Hit
AGCTATGGGCGATCTTGTTCGGGGGACCAAATTGCAGACTAATTGTTTCCAT1630.10901332905305539No Hit
TAAGCTGGGTATTTCTATTTCTAGATGCCTGTTACAAAACATAGAAATTATC1630.10901332905305539No Hit
ATTCTTGGGGGAATTTAAATAATTCCTATTTGCAAATTAGAATGACAGTGTT1620.10834453562328203No Hit
CACAACGGGGATGAGCTGGCTGGGAAAATGAAAGACCTGAGTTTGGGAGGTG1620.10834453562328203No Hit
ATACAAGGGTATTTGGAAACTCTAGTGAAAAGCTAATTTCTAGATTCTAAGG1620.10834453562328203No Hit
TCACTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1610.1076757421935087No Hit
GTTTATGGGTTTTCAACTACTTTCTCTACAGCTTTTCATGTAAATTAGTCTT1610.1076757421935087No Hit
TCTTCTGGGATCCGGCAGAGGGAGGAAGGCAGAGCAAGTGGAGCAGGGCTTA1610.1076757421935087No Hit
GAAAATGGGCTACTTAAGTGGAAAGTGACTGAAAGATCTGGAAAAGTGGCTT1610.1076757421935087No Hit
TTAAATGGGGATAGCTGATCATGGTTATTTTATTTTTCTAATTACTAGTGAG1610.1076757421935087No Hit
TCAGCGGGGGACTCTACTTCAGAGGAAGTTGCTCTCTGGGAGAAGATGGTAA1600.10700694876373536No Hit
TGGTATGGGAATATAATTGCAAATAGGTAGGAGTGGCCTAAATTATCTAATG1600.10700694876373536No Hit
GTGCCTGGGGGAGACAGATGCGAGGCGGCGGTCAGGTGGGATGCTATGGAAT1600.10700694876373536No Hit
GTAATAGGGCAATGTTAAATCACAGGAGGGGAAGCTATTGCCATATGCCTCT1590.106338155333962No Hit
AAATCAGGGTCTTGCGCGGGGAACTGAAATTAATGTAGTATTGCTATTAGGC1590.106338155333962No Hit
CCAGCAGGTGTCTAAAGGTTCTGTTTTCCATTTTCTGGTGTTAAACCTGAAT1590.106338155333962No Hit
CACAGGGGCTGGGAAATGAGATGACCACTTTTTAGAAAGATAATTCACTGTA1590.106338155333962No Hit
TCTGATGGGTCCTCTCTTCTGTAGGAGCTTTGGGTTTTCAGAGGTGCAGGGA1590.106338155333962No Hit
AGTGTTGGGATTATTACCAGGATTTTAAACATTTTGAGGGTTTTTCTGTAGA1590.106338155333962No Hit
AACCTTGGGGACTCCGAGGGATCGTTCCTAAATCCCACCACCGGGAGCAGAG1580.10566936190418866No Hit
GGCGAAGGGATAGCGAATGCACCTGTGCGTTGAAGGCTGTTTCGCTGTAACA1580.10566936190418866No Hit
GCTCATGGGAGGGGAGTGAAGTTCTTCAGTGATGTTTACTCTTCTCCTGATA1580.10566936190418866No Hit
TATAGAGGGATCCCTGTTTATTGGGAAGGATATGTTCGATTCTTAAATACGC1580.10566936190418866No Hit
ACTTCAGGGCAGACCCCAGGCTTAAGTGATGATGAGTTGGCCAGCTTGGTTA1580.10566936190418866No Hit
GTGCCAGGGCTGTAAAAAATAGCTTCCTGGGGAAAACTAGAGGATAGAGGTC1580.10566936190418866No Hit
TCAGCAGGGAAAAGCTATGGGGGGTGACAGATATATTCATTATTTTAATTGT1580.10566936190418866No Hit
TTGCCTATGTTTTTAAGATAAAATCTCCCTCCCCTTCGAAAATCAAATTAGG1580.10566936190418866No Hit
GGAACGGGGATGGCAGAATACAGAAAATGCTTTATTGGAGACAGTCATTGTA1580.10566936190418866No Hit
ATTTCCGGGGCAGAAAATATCCATCAATCTGTCTGAGTGGTCCCTGTAAAAT1570.1050005684744153No Hit
GAAGCAGGAACCCAGGCTCCAGAAGGTGTTGCCAGGGGCCCAGATGCCCTGA1560.10433177504464196No Hit
AACACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10433177504464196No Hit
CTACCAGGGCAGATGCAAAATCAGATAATTTTATTTTGTTTCATGGGTTTTC1560.10433177504464196No Hit
GTAATAGGGTTGATCCAGTATTTCAACACAAATTTACTTTGCTCATAAAATG1560.10433177504464196No Hit
CTTGAGGGGGGGTTTTCCTGTAAGTCGTATTAATAATTCTCTAAGGGGACCC1550.10366298161486863No Hit
ACTACTGGGACCAAGGATGCAGCTTGACTTTAAATCCATTCATGTTCAAGAA1550.10366298161486863No Hit
TGTCACGGGGCAGAGTTCAGACTTAGGAGTAAAACAGAAAAGTGAACTAAAC1550.10366298161486863No Hit
AGAAACGGGCCACAGCCCTTCTGAATTTCTCACAAGGTAATCTACCTAACAG1550.10366298161486863No Hit
TCTCGAATTGGGCGATCTTATCGCGGGAAATGAATTTGAGTTATCAAAAAAC1550.10366298161486863No Hit
TTCTGCGGGGTAACTAAATTGAGTTCAAATTCTATCCCAGCTTAGACTGGTT1550.10366298161486863No Hit
AGTTGCGGGCTATCAGGGCGCTGTCAGACTGGCTGCTGGAGCCCAGATGGCA1550.10366298161486863No Hit
AGAACGGGCATCAGAATCACGCTCCAGATCAAAGTCAGCTTCAAAAACCCGA1550.10366298161486863No Hit
ACGTTGGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC1540.10299418818509526No Hit
TGACATTGGGAGAAGAGTATTAATATGAAATAATGAATAAGGACATTAAAAG1540.10299418818509526No Hit
TATCTCGGGGTGGCTTAAAACGCTGATTGCCATTTGCATTGTCTACTGAAAA1540.10299418818509526No Hit
ATACAAGGGGTGCTAGTTACTCTAAGAAAAACACCAAGTCTCAGTGGCTTCA1530.10232539475532193No Hit
AAATAGGGATCTACTTCATAAATTTATCATTATCTTTTTATAACTATTAGAA1530.10232539475532193No Hit
TTACTAGGGAATACATAGTTCAATTCAGATTTCTCTTGTCTGAAAGATTCAC1530.10232539475532193No Hit
TTGCTAGGGAGTCTTCCTCGATCCCGTGGTGCTCCGCGGCGCGGCCTTGCTC1530.10232539475532193No Hit
ATAAATGGGGTCACATGTGTGTACCATACAAATGCTGTCTCAAGTGTACCAT1530.10232539475532193No Hit
GGCTTTGGGTACTTATGTGTTGTATGTCAGTGTAACATGCAGATGTATATTG1530.10232539475532193No Hit
AGCAGCACTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTA1530.10232539475532193No Hit
AAGCTTGGGTATTGCTGATGTTCATTTGGTCACCTGGCTGATGTTTAACAGG1520.10165660132554857No Hit
TATTCAGGGGAAAAAGATGTGAGTCCCTGTTGATTCAGGAAGTCTTTTATAA1520.10165660132554857No Hit
TAGCAAGGGATTCCTTTTAGCATTACTATTTTTTGTTAAGAATTTGGACAAA1520.10165660132554857No Hit
TTTTAGGGGCAAGTGAAAAGAAATATGAAGACAAACGAACATATGTAAGTTA1520.10165660132554857No Hit
CAGCTTGGGTCCAGAAGCTATTGTCTATTCAATTCAAAATCTACCTGAAGAA1510.10098780789577523No Hit
CTAAAAGGGGTCACGGCCATCAGCTTACACAACATCAGAAAACGCACAGTGG1510.10098780789577523No Hit
ACAGCGGGGGCTGAAAAATAGGACCCAACTATATGCTACCTACAAGAGATTC1510.10098780789577523No Hit
GGTAATGGGTGAAGACGCTGTTTGAGCAAGCCAAGCATGCCAGCACCTACCG1500.10031901446600189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)