FastQCFastQC Report
Sat 31 Dec 2022
SRR5844139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158226
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC6550.41396483510927407No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5160.32611580903264953TruSeq Adapter, Index 2 (95% over 22bp)
GAAGCGGGTACTTAAGGTAGGCTCGGGAGAAAAAAATACAAAACTAATCATA2560.1617938897526323No Hit
CCTACCGGGTTGGAAAGAAAATCCTAGAAAGCCTATCACCATAGATCAGCTA2340.14788972735201547No Hit
TGGTTTGGGATGTTTTTTGGCCATTTGTTGTGTAGCTTCTTTGAATAAATGC2200.13904162400616837No Hit
AAAAGAGGGATGCAATGAAAGTGCCGGTGAGAATTTAGACAGCAAAGCTTAC2170.13714560186062974No Hit
TTGCTAGGGGAGAATACAGACAGTAGGATCTGCATCCTTACTAGCATATAGA2160.13651359447878353No Hit
TACACGAGGGATTGCTAAGTATTCTTAAACATCGACTTCAGAAATCAAAATT2090.13208954280585997No Hit
TATGTCGGGACTGTGCTCGTTTTGTTGAATTGGTACATCAACAGAAAACTCC2080.13145753542401376No Hit
TCTCAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC2080.13145753542401376No Hit
TAGACTGGGCCAATGAGTGGCAGAACCAAGACCGAAAGTCTGAGTTTTCAAG2070.13082552804216754No Hit
GTTGCTGGGCAACCCTGGGTGTGACTGACTTCTTAGATTTTATCAATGAGAT2040.12892950589662885No Hit
TTTAAAGGGTATAATTTTGGATGTAGACATTTAAACTAGATTCCCAGTCCTC2040.12892950589662885No Hit
ATCAACGGGGATCTACGCCGGGGGAAGGGTGGTAGGGAGGAAGTAATATAAT2000.12640147636924398No Hit
GACTCAGGGGGTAGAAACAATACTTTTAAAGTGCTAAAAGAAAAGAGCTGTG1990.12576946898739777No Hit
ACAGATGGGTACAGGCGCGAGCCACTGTACCTGGCTAGTTGTGGTAGGATAT1990.12576946898739777No Hit
GAAAGGGGGGTTTTATAAAGTAGTGCTTGCCGGGCATGCTGGCTCACTCCTG1990.12576946898739777No Hit
TTGGCAGGGGGTGCTTGGGAACTGCTGGTTATGAGCATTCCTGGTTTTCTTC1980.12513746160555156No Hit
CACCACGGGAGATAATCTTGTGCTTCCAGATTGTTGGAATGAAAAACAAGCA1980.12513746160555156No Hit
TAAAGTGGGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAA1980.12513746160555156No Hit
TCATGAGGGACTTGCTTACATAAACAGAAATTTAACAGCCCATATTCTTGAA1970.12450545422370535No Hit
TGCTTGGGATTAAGATGATTGTCAGTACATTAAACTTTTCAATCCCATTATG1950.12324143946001288No Hit
TGCTAGCGGGATAGCAAAAGCTCAACAAATACTTGATGAATGAATAATTCCA1930.12197742469632045No Hit
GTGGCTAGGGGAGTCTTGGCGGAAGCCTGAGACGCAATAGATGGAGACTCTC1930.12197742469632045No Hit
TGCTTGGGGATGTTTCTCTGGGGGCTGAAAGAGTGACTCATGTCTGGGAATG1900.1200814025507818No Hit
ACTGTAGGGGATGCTCCATCCAGCTACTACTTTGGGGTTTATCTGGGAGTAG1900.1200814025507818No Hit
ATTAGAGGGGTTCCACATTAAATGCATTTTCATTATAAAAACTTGAAAAAAT1900.1200814025507818No Hit
GACATGGGGCAACCAGGAAGACTACAGGCTTTTATCTGACTCTGGCCACACT1890.11944939516893559No Hit
GACCATGGGACACACGTGTGCTCCCTGCCCTGCTCCTGGCCCCTTGGCCGGC1890.11944939516893559No Hit
TGACTGGGAACATCCTCCACTGACTTCCTCCCACTGTCTGCCCTGAACCCAC1880.11881738778708935No Hit
GCATTGGGAATGTTTACTTTCCTTGTCCTGTATTATTATATCTCTCAGGATT1870.11818538040524312No Hit
TGTTAAGGGTATGAAGGAAGACCATGAATCATAAGTGCAAGAACTCCAGACA1860.11755337302339691No Hit
GCCTGGGCGACGGAGTGAGACTCCATCTCAAAAAAAAGAAAGGAAAAGAAAG1860.11755337302339691No Hit
GCCATGGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1860.11755337302339691No Hit
AGTAAGGGCTCTAGGAAATTATTGGCCTGCTTTCTGTCACTATCAATTAGCC1850.11692136564155069No Hit
TCTCTAGGGGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAA1850.11692136564155069No Hit
GCTCTTGGGAGGTTGTCCCTAATTTGTCAGAGGCTGTAGTACCATATTTTCT1840.11628935825970448No Hit
CACTGTGGTATAGATTCCATAATATGCTTGAATATTATGATATAGCCATTTA1840.11628935825970448No Hit
CGAAGTGGGACCGAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAC1840.11628935825970448No Hit
TCATACGGGGTGCTCGGTGCGCTGAGCTCCGCGGCTCCGCGAGCCGGTTCCG1840.11628935825970448No Hit
CTTGCAGGGCCCGGACGGAGGTAGAGGCCAGGGCAGCGCGTCCGGGAGCGGA1840.11628935825970448No Hit
GCTAGCGGGATTTTTTCAACTCTGAAAATAACAGGAAACATCCAATTTTGCC1830.11565735087785825No Hit
GCGCTTAGGGACTCTTAACTCCTTGAGGGCACACAATTTTACTTATTGGTCC1830.11565735087785825No Hit
GGAGCTGGGGAGCAGAAATGGCGGCTGCGGCGGCTCGAGTGGTGTTGTCATC1830.11565735087785825No Hit
TATGTGGGGAAACCCTTACTAGTGATTGTCCCTGAGGAGAGAACTTGTGGCT1820.11502534349601204No Hit
TAACATGGATGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAAC1820.11502534349601204No Hit
GCGCTGGGGGTGATGTTCCTCCTTCTATGTCAGGAAGCTGTGCTGTGTGTGT1820.11502534349601204No Hit
AACTCTGGGATTGAAACTTCCCCCAAATCTGATGGACCTAGAAGTCTGCTTT1810.11439333611416583No Hit
TTTGATGGGCCGATCTTAAGCCGGGAAAAGTAAAAAACTTCCCAACTCACTT1810.11439333611416583No Hit
GTCGATGGGGCTCAGTATTAGTAGACAAGGTCTTTGTTCAGACGATTAGGTG1800.11376132873231959No Hit
TGGCCTTGGGCATCAAATAAAGCAAAACCTTGCAATATCAGCTAGATTTACA1790.11312932135047336No Hit
ACTGCAGGGCGCACGGGGAGGCCCGAGGAGCGGGCCCCCGAGTCCAAGTCCG1790.11312932135047336No Hit
CACACAGGGATCAAAAATAGAAAATTCATTTTATATTTCTAGCTGAAAATAA1770.11186530658678093No Hit
AATTAGGGGATCGCTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAA1770.11186530658678093No Hit
CAAACCGGGAACTGAATAATGGCTTTTTGCATGGAAAAAATAGTTTTTACTA1760.11123329920493472No Hit
GTAAATGGGGGCTCCATGGTGTCCAAACAGCCTATTGAAATCTATGGCCCTG1750.11060129182308849No Hit
ACGGCCGGGAGATAGGAATTGTTCGCCAGTTCCCATTTTCTTCTGCTTTGCA1750.11060129182308849No Hit
GGCGCAGGGGGGCAACAAAGCTAATGTGGGTTCTCCAAGATTAACACAACTG1740.10996928444124228No Hit
AGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAA1740.10996928444124228No Hit
ACTCTGGGGATGATGATATGGCTGCAGCCATGAAGGCAGTCACAGAACAGGG1730.10933727705939605No Hit
TAGCTAGGGGGGGAATGAAGTGAGGCCAGTGGCCGGGTCTGCTGAGTCCGCA1730.10933727705939605No Hit
TAGCAGGGATAAGGAGAAAGTCACCAACAGCTTGATCTATTATTTTTCCCTC1720.10870526967754984No Hit
TTTACAGGGGAATAGTATTTTTATAGCTTGTTAAAGACATGGCTAGTTGCAT1710.1080732622957036No Hit
CTTGATGGGGGACTCATGGACTTACACCAATGGTTAGCCAGGAGCTCTCAGG1710.1080732622957036No Hit
AGTCCCGGGGTTCTTGTTGTCATCATGGTACCAAGGCTTCACAAACTAATAA1700.10744125491385739No Hit
TGTATCGGGGAATGTTTTTATTTATAATGAGCATACCAGTGTGTAGCCTCTT1700.10744125491385739No Hit
CAACAAGGGATGAATGAAGCAGGTGTTGTAGAAGGTCAGTCAGTTAGACCAT1700.10744125491385739No Hit
TAGCATGGGATATAATTTGTATGAATTTATAATTCACGAAAATCTCAAAAAT1700.10744125491385739No Hit
GTAAAAGGGGAATGGATGTTGCGGCAGCAAGGAAATTAGACAAGGAGCACAG1690.10680924753201117No Hit
CACCCGGGGAAGTAGTTTTTACTTGAGTTATGTCAAATTTCCACTCTTAGGA1670.10554523276831873No Hit
TATCTAGGGGGTAAAGGTTCTTAGATTAACTCTGCATTTAACTTTATTTGCA1660.10491322538647252No Hit
GCGCTTGGGACCTGTGGTCTAAACCCCAAGTCATTATGTCTTTCTCAAGCTG1660.10491322538647252No Hit
CTTTTATCGGGGCAGAGCCGGCGCCGGAGGAGACGCACGCAGCTGACTTTGT1650.1042812180046263No Hit
GAAATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1650.1042812180046263No Hit
TATGGCTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG1650.1042812180046263No Hit
TGGGTTGGGCTTCCGATCTTGATAAGGGCAAGAATTGTTGTGTAACTCAAAG1650.1042812180046263No Hit
CCTTCAGGGGGAGTTAGAGAATAATACATTTATAGAAAAGACAATCACATTT1650.1042812180046263No Hit
TTGTAAGGCAAAGAAGACTACAACACCAAAAGGTAAAGGCCGAGGGGCAAAG1640.10364921062278008No Hit
GTGTGTGGGAACTTACCACAACCTGTTTCCTGATTCTATGACAGCAACACAG1630.10301720324093384No Hit
TCGCTGGGCTGCATAATGAATTAACTAGAAACAACTTTGCAAGGAGAGCCAA1630.10301720324093384No Hit
GTCCAAGGGCAACCATGCCCAGCTATTTTTTGTTGTTGTTGGTTTTTTGTAG1630.10301720324093384No Hit
TAGACTGGGGTCATTGAGAGATGTAAAAACTAAAACCCAAAGCTGGCAATAC1630.10301720324093384No Hit
AACAACGGGATAGTTTAGTTTTGAAACAAAGATGATGATAGTCCTTTCCCAA1630.10301720324093384No Hit
TACTGGGGGAAGTTAAAGGTGTTTTCCTTCCTCTACCATATACAATTTTTAA1630.10301720324093384No Hit
TAACGTAGGGATTTGTGGAATGAATGAGCCACACTTTTTTTTTTTTTTTTTT1620.10238519585908763No Hit
GCTATTGGGCATTTGGAGCAATGTGATCAGTTTGCATTTAAAAGGAAAAAAA1620.10238519585908763No Hit
CACACTGGGTTGTTATTTTTATTTTTACAGATGAAGAAACAGCTCAGATTTG1620.10238519585908763No Hit
TATCAAGGGATTTTAAGGCATTACAATCTTAACACAAAGAGTTTCATATCTT1620.10238519585908763No Hit
GTAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1620.10238519585908763No Hit
GCTAGGGGGGACAATCTCTTCAATAAATGGTGCTGGAAAACTGGATATCCAT1620.10238519585908763No Hit
TATAGCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1620.10238519585908763No Hit
GTGCATGGGGTGGAACACTTGCAGGAAAGGTGAACACTGCCAGGACCAATTA1610.10175318847724141No Hit
TGTTACGGGCATCCCAGCCCCCGGTTGGAGCCTGCTCTTTGAACTCCGCTGC1610.10175318847724141No Hit
GGTCATGGGATTGCGAGAATGCTGACACAACTACCATCCAAGAGATTTCATA1610.10175318847724141No Hit
TTTACAAGGGCTAAGCATTCCTATTTTTTCCTAATTTCCTTATTTGTATTGC1600.1011211810953952No Hit
TGCTGGGGGGGGCGATATATACAGCCTGGATGTGCTGGACAAAGGGATGATT1600.1011211810953952No Hit
CAGGCTGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1600.1011211810953952No Hit
AGCCTAGGGAAAGGAGGGCCAGGTTAATGCATGACTTTAAGGATCAACCTTC1600.1011211810953952No Hit
CGCTTGGGTAGGGGATGATCCAGGTCTTGGGATTTTCAAAGGTCCCCATGTG1600.1011211810953952No Hit
AGACATGGGTGATACTGCCCTGTTGTTGTTATGATTAATAGCAATTATGTTT1600.1011211810953952No Hit
GTTTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.1011211810953952No Hit
TGCTTTGGGACTTTTCAAACATGATGCACTTTTATCTTTGTGAATAATTTAC1600.1011211810953952No Hit
GGCTAAGGGCTGAGTGACCTACAAAGAGACTTAGACTCCCACACATTAATAA1600.1011211810953952No Hit
AAGGCTGGGCTTGCTTTAGTGTGTTGGTATGTTCATAGGAATGGATCGCTCT1590.10048917371354897No Hit
TAGCTTGGGGATCTTCAAAGTTATAACTTTGAAGATCTCCCCAATAACATGG1590.10048917371354897No Hit
CGAAATGGGAAATCAACAGAATATACATTCTTCTCAGCACCACATAGCACTT1590.10048917371354897No Hit
TGCCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1590.10048917371354897No Hit
AAAAGCGGGACTTCACACTGAGACACTTGGACGTCACATGTGAAGCCTACGA1590.10048917371354897No Hit
GTGCACGGGAAGTAGCGGCGGCGCTTCAAGATGCGCTGCCTGACCACGCCTA1590.10048917371354897No Hit
GTAACAGGGCACTATAATATGACAAGTAATTGGCAGAAAATATGAATGCTTT1590.10048917371354897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)