FastQCFastQC Report
Thu 19 Jan 2023
SRR5844138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158116
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5870.37124642667408736No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5580.35290546181284627TruSeq Adapter, Index 2 (95% over 22bp)
GAAGCGGGTACTTAAGGTAGGCTCGGGAGAAAAAAATACAAAACTAATCATA2540.16064155430190494No Hit
GTTGCTGGGCAACCCTGGGTGTGACTGACTTCTTAGATTTTATCAATGAGAT2350.1486250600824711No Hit
ACGGCCGGGAGATAGGAATTGTTCGCCAGTTCCCATTTTCTTCTGCTTTGCA2340.14799261301829036No Hit
TAAAGTGGGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAA2310.1460952718257482No Hit
GACCATGGGACACACGTGTGCTCCCTGCCCTGCTCCTGGCCCCTTGGCCGGC2270.14356548356902527No Hit
AAAAGAGGGATGCAATGAAAGTGCCGGTGAGAATTTAGACAGCAAAGCTTAC2220.14040324824812164No Hit
TATGTCGGGACTGTGCTCGTTTTGTTGAATTGGTACATCAACAGAAAACTCC2170.137241012927218No Hit
CACCACGGGAGATAATCTTGTGCTTCCAGATTGTTGGAATGAAAAACAAGCA2150.13597611879885654No Hit
TCATACGGGGTGCTCGGTGCGCTGAGCTCCGCGGCTCCGCGAGCCGGTTCCG2150.13597611879885654No Hit
TGCTAGCGGGATAGCAAAAGCTCAACAAATACTTGATGAATGAATAATTCCA2140.13534367173467582No Hit
TATGTGGGGAAACCCTTACTAGTGATTGTCCCTGAGGAGAGAACTTGTGGCT2140.13534367173467582No Hit
TTGCTAGGGGAGAATACAGACAGTAGGATCTGCATCCTTACTAGCATATAGA2140.13534367173467582No Hit
GCGCTTGGGACCTGTGGTCTAAACCCCAAGTCATTATGTCTTTCTCAAGCTG2050.12965164815704927No Hit
AGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAA2050.12965164815704927No Hit
AGTAAGGGCTCTAGGAAATTATTGGCCTGCTTTCTGTCACTATCAATTAGCC2040.12901920109286855No Hit
TAGACTGGGCCAATGAGTGGCAGAACCAAGACCGAAAGTCTGAGTTTTCAAG2030.1283867540286878No Hit
ATCAACGGGGATCTACGCCGGGGGAAGGGTGGTAGGGAGGAAGTAATATAAT2030.1283867540286878No Hit
TGTTAAGGGTATGAAGGAAGACCATGAATCATAAGTGCAAGAACTCCAGACA2010.12712185990032634No Hit
TGGCCTTGGGCATCAAATAAAGCAAAACCTTGCAATATCAGCTAGATTTACA2000.12648941283614562No Hit
TGCGCGGGGGGAGTTCTGGCCGACAGCAGGCGAGGAGTGGGTAGCAGCGCCT1990.1258569657719649No Hit
CAACAAGGGCTCTCATGATTTTGATTTGTTTTTTTTCTTTTCAGTGAAACTA1980.12522451870778417No Hit
TTTAAAGGGTATAATTTTGGATGTAGACATTTAAACTAGATTCCCAGTCCTC1960.12395962457942271No Hit
ACAGATGGGTACAGGCGCGAGCCACTGTACCTGGCTAGTTGTGGTAGGATAT1950.12332717751524197No Hit
GGAGCTGGGGAGCAGAAATGGCGGCTGCGGCGGCTCGAGTGGTGTTGTCATC1950.12332717751524197No Hit
AATTAGGGGATCGCTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAA1940.12269473045106125No Hit
CTTGCAGGGCCCGGACGGAGGTAGAGGCCAGGGCAGCGCGTCCGGGAGCGGA1930.12206228338688052No Hit
TCTCTAGGGGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAA1920.1214298363226998No Hit
AACTCTGGGATTGAAACTTCCCCCAAATCTGATGGACCTAGAAGTCTGCTTT1920.1214298363226998No Hit
GCGCTTAGGGACTCTTAACTCCTTGAGGGCACACAATTTTACTTATTGGTCC1920.1214298363226998No Hit
TGGTTTGGGATGTTTTTTGGCCATTTGTTGTGTAGCTTCTTTGAATAAATGC1910.12079738925851906No Hit
GACTCAGGGGGTAGAAACAATACTTTTAAAGTGCTAAAAGAAAAGAGCTGTG1900.12016494219433833No Hit
GGCGCAGGGGGGCAACAAAGCTAATGTGGGTTCTCCAAGATTAACACAACTG1900.12016494219433833No Hit
GTGGCTAGGGGAGTCTTGGCGGAAGCCTGAGACGCAATAGATGGAGACTCTC1900.12016494219433833No Hit
ACTCTGGGCGATCTGCCTAGGGGAGGGGCATCAACTCCAGCTGGATTATTTT1900.12016494219433833No Hit
GCTCTTGGGAGGTTGTCCCTAATTTGTCAGAGGCTGTAGTACCATATTTTCT1890.11953249513015761No Hit
TCGCTGGGCTGCATAATGAATTAACTAGAAACAACTTTGCAAGGAGAGCCAA1890.11953249513015761No Hit
ACAAGGGTTCATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTG1890.11953249513015761No Hit
ACTGCAGGGCGCACGGGGAGGCCCGAGGAGCGGGCCCCCGAGTCCAAGTCCG1880.11890004806597688No Hit
CTATCATCGGGTAGAAACTAATGTGAAATCCACAAAACTACTATTAATTTTT1880.11890004806597688No Hit
CAGGCTGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1870.11826760100179615No Hit
CGAAGTGGGACCGAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAC1870.11826760100179615No Hit
TAACATGGATGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAAC1870.11826760100179615No Hit
ACTCTGGGGATGATGATATGGCTGCAGCCATGAAGGCAGTCACAGAACAGGG1860.11763515393761542No Hit
TCTCAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC1850.1170027068734347No Hit
CCTACCGGGTTGGAAAGAAAATCCTAGAAAGCCTATCACCATAGATCAGCTA1840.11637025980925396No Hit
TAGCATGGGATATAATTTGTATGAATTTATAATTCACGAAAATCTCAAAAAT1840.11637025980925396No Hit
CACCCGGGGAAGTAGTTTTTACTTGAGTTATGTCAAATTTCCACTCTTAGGA1820.11510536568089251No Hit
GTCAAGGGGGATTCCTTTCCGTGTCAATGATGGCTTTTAATCCCAATTTCCA1820.11510536568089251No Hit
TTGTAAGGCAAAGAAGACTACAACACCAAAAGGTAAAGGCCGAGGGGCAAAG1820.11510536568089251No Hit
GAAACAGGATAAAACTGATGTGTCAGTTACGGAAAAATTAGAAATGCAGCAC1820.11510536568089251No Hit
TTTGATGGGCCGATCTTAAGCCGGGAAAAGTAAAAAACTTCCCAACTCACTT1810.11447291861671179No Hit
CCATCCGGGATGGGCACTATTTTGAATTCTAGAGATGGTTTTTGTTAAATCT1810.11447291861671179No Hit
GCTTGCGGGTGTAGAGAGCAAATAAGAATACATATTTATATTTGTTTGTGTT1800.11384047155253106No Hit
TTAGTGGGGGCCTTCTAATTTATACTCAAACCCTATGAATTTCCTTAAAAAT1790.11320802448835032No Hit
GAAATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1770.11194313035998886No Hit
TCATGAGGGACTTGCTTACATAAACAGAAATTTAACAGCCCATATTCTTGAA1770.11194313035998886No Hit
TTTACAGGGGAATAGTATTTTTATAGCTTGTTAAAGACATGGCTAGTTGCAT1760.11131068329580814No Hit
CAGTGGGGTAAACATTCTCTAAGCACCAAATTTGGTAGGGATTTCCACTTCC1760.11131068329580814No Hit
TGTAAGGGACAACTGGTGCTCAAGATGACTTGAGAAAAGTAACTCAGAGTGC1760.11131068329580814No Hit
CAAACCGGGAACTGAATAATGGCTTTTTGCATGGAAAAAATAGTTTTTACTA1750.11067823623162741No Hit
TGCTTGGGGATGTTTCTCTGGGGGCTGAAAGAGTGACTCATGTCTGGGAATG1740.11004578916744669No Hit
CATTCTGGGCAGTAGCCCAGGATTGCTACAAAAAGGTCCTTTGAGAAAATTA1740.11004578916744669No Hit
GACATGGGGCAACCAGGAAGACTACAGGCTTTTATCTGACTCTGGCCACACT1740.11004578916744669No Hit
AGTCCCGGGGTTCTTGTTGTCATCATGGTACCAAGGCTTCACAAACTAATAA1730.10941334210326596No Hit
GCCTGGGCGACGGAGTGAGACTCCATCTCAAAAAAAAGAAAGGAAAAGAAAG1730.10941334210326596No Hit
AAACCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1720.10878089503908524No Hit
TATGGCTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG1720.10878089503908524No Hit
GCCATGGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1720.10878089503908524No Hit
TGACTGGGAACATCCTCCACTGACTTCCTCCCACTGTCTGCCCTGAACCCAC1720.10878089503908524No Hit
TCATTTGGGATACTGGAGCTCATAACCCCTACTCCACAGCTGTGAGTACCTC1710.1081484479749045No Hit
TAGCTTGGGGATCTTCAAAGTTATAACTTTGAAGATCTCCCCAATAACATGG1710.1081484479749045No Hit
TGCTTGGGATTAAGATGATTGTCAGTACATTAAACTTTTCAATCCCATTATG1710.1081484479749045No Hit
TGTATCGGGGAATGTTTTTATTTATAATGAGCATACCAGTGTGTAGCCTCTT1700.10751600091072376No Hit
GTCGATGGGGCTCAGTATTAGTAGACAAGGTCTTTGTTCAGACGATTAGGTG1700.10751600091072376No Hit
TCGTTGCGGGGGGCACAGGGCCAGTGAGGCCGGCCACGCTCGGGCCCTCACC1700.10751600091072376No Hit
GGTGGGGATGTGTGAATTTGATCCTGTCATTATGATGCTAGCTGGTTATTTT1700.10751600091072376No Hit
ACACTTGGGCAAAGATGAGATAAAACATTGTCCCTGCCCCCGGGGGCTCACA1700.10751600091072376No Hit
TGGACTCGGGATTTTTAAAATTAGTTGAGCATGGTGGCATATACCTGTAGCC1700.10751600091072376No Hit
TCTGCTGGGAGAGGGCAGGTGTTTGTTTGCCAGTGGCAGTCCATTTGGGCCA1690.10688355384654304No Hit
TCAGAGCGGGCACAGCTTGTAAGTATCAGCATTTAAACTTGCATTTCCACCT1690.10688355384654304No Hit
TTTCTAGGGCTCCCATGGAACCGGATGCTGCAAACCCAGAATGCAGCCTTCC1690.10688355384654304No Hit
CGCTTGGGTAGGGGATGATCCAGGTCTTGGGATTTTCAAAGGTCCCCATGTG1690.10688355384654304No Hit
ATTAGAGGGGTTCCACATTAAATGCATTTTCATTATAAAAACTTGAAAAAAT1690.10688355384654304No Hit
TACACGAGGGATTGCTAAGTATTCTTAAACATCGACTTCAGAAATCAAAATT1690.10688355384654304No Hit
GTCCAAGGGCAACCATGCCCAGCTATTTTTTGTTGTTGTTGGTTTTTTGTAG1680.10625110678236231No Hit
TTAAACGGGACCTCAGGCTACATCCACATGAGCCTGTTCATGTGGTTTCTCC1680.10625110678236231No Hit
TCACGTCGGGCATGAATGTTTATAAAAGAAATATGCACATGCACAGTTGAGC1670.10561865971818159No Hit
TCATGAGGGATACATGAACCTAAAGGAAAGATTTTCTGTAAATATTTTAAGT1670.10561865971818159No Hit
CGCTAAGGGCGATCTAGACTGGGGGGTCCGGGTCATTGTACACTGAAGCCAT1670.10561865971818159No Hit
AAAAGCGGGACTTCACACTGAGACACTTGGACGTCACATGTGAAGCCTACGA1670.10561865971818159No Hit
GATTTAGGGGGGAAACAGCGCGGGGTCCGCTATGGCGGCGGCAGCCGAGGGC1670.10561865971818159No Hit
TTTGCAGGGCTTTCTTTGCTGCGTCTACTGCGAGAATGAAGACTATTCTCAG1650.10435376558982014No Hit
TGTTACGGGCATCCCAGCCCCCGGTTGGAGCCTGCTCTTTGAACTCCGCTGC1650.10435376558982014No Hit
CGCAATGGGATAGCACTGAACAGGGAGTCAGGAGACTATTGTCTCCTAAACC1650.10435376558982014No Hit
TAGACTGGGGTCATTGAGAGATGTAAAAACTAAAACCCAAAGCTGGCAATAC1650.10435376558982014No Hit
TGGGTTGGGCTTCCGATCTTGATAAGGGCAAGAATTGTTGTGTAACTCAAAG1650.10435376558982014No Hit
CTGTACGGGCAACATCTCCCTTTACAAAGTTAAAAATTTTGACCCTGACTCT1650.10435376558982014No Hit
CTGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1650.10435376558982014No Hit
TTTTTGGGGGGCAGAGTGACAGGTGAGAGGGGTGGGACGTTCACTGTAGTCT1640.10372131852563941No Hit
TACAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1640.10372131852563941No Hit
GGCCTAGGGAGATCTGCTTGACTAGTGACTGCTTTGGATAACTTTTTTAAAC1630.10308887146145866No Hit
CAGCTCCACTTTGCAATCTTGTATCAAAGAGAGAGCATGCTGTCAGTTTCTG1620.10245642439727794No Hit
GCTAGCGGGATTTTTTCAACTCTGAAAATAACAGGAAACATCCAATTTTGCC1620.10245642439727794No Hit
CACACAGGGATCAAAAATAGAAAATTCATTTTATATTTCTAGCTGAAAATAA1620.10245642439727794No Hit
TTGTATGGGATATTTAAAGAAAATAAAAGTTTGTTTTATATTTTAGTTTTTG1620.10245642439727794No Hit
CACACTGGGTTGTTATTTTTATTTTTACAGATGAAGAAACAGCTCAGATTTG1620.10245642439727794No Hit
AACACAGAACCCCACTGGAACGACACAGAGCCGGCCTGCAAAGCCATGTGTG1620.10245642439727794No Hit
GTGTGTGGGAACTTACCACAACCTGTTTCCTGATTCTATGACAGCAACACAG1610.10182397733309721No Hit
GACTCTGGGGACGCTCTTCCGATCTAATCATGGGAGATTCATGAGTAGGTAA1610.10182397733309721No Hit
GACATAGGGGACTATCTCCATCAGAGATGGCAAGGCCACAGGTGATCTGAGA1610.10182397733309721No Hit
GCATTGGGAATGTTTACTTTCCTTGTCCTGTATTATTATATCTCTCAGGATT1610.10182397733309721No Hit
AGCTCTGGAAATACCTCAAAGCTGCTCCCTGCAGGCCACTCTGTATCAGGCT1610.10182397733309721No Hit
CAACAAGGGATGAATGAAGCAGGTGTTGTAGAAGGTCAGTCAGTTAGACCAT1610.10182397733309721No Hit
TAACGTAGGGATTTGTGGAATGAATGAGCCACACTTTTTTTTTTTTTTTTTT1600.10119153026891649No Hit
TTGGCAGGGGGTGCTTGGGAACTGCTGGTTATGAGCATTCCTGGTTTTCTTC1600.10119153026891649No Hit
TTGAGTGGGAACTCCCCCTGGATGAGGCCCAGAGACGAAAGCTGGATGTCTC1600.10119153026891649No Hit
CGCCGGGGATTCAAGGGGTTCTCCTGCCTCAGTCTCTGGAGTAGCTGGGAGT1600.10119153026891649No Hit
GTTTATGGGCAATAGAAGGATGTACATTTCCAAATTCACAAGTTGTGTTTGA1590.10055908320473576No Hit
TTTGCGGGATGCCTCCAGCTTTGTTCTTGTTACTTAGGATTGCCTTGGCTAT1590.10055908320473576No Hit
GGCCCAGGACTTGATTACATCACTGTAGAATTCTACCAGACATTTAAAGAAG1590.10055908320473576No Hit
ACTCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1590.10055908320473576No Hit
AACAACGGGATAGTTTAGTTTTGAAACAAAGATGATGATAGTCCTTTCCCAA1590.10055908320473576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC253.250623E-546.359414
CAGGTTG253.250623E-546.359415
GATAACC253.250623E-546.359411
TTTACTC253.250623E-546.359416
CACGGCG253.250623E-546.359415
ATGGAAC359.519317E-846.359415
GACCGTC253.250623E-546.359416
ACCGAGG253.250623E-546.359410
CACTGGT301.7544535E-646.359414
ACCCTTA359.519317E-846.359412
TGACGGT253.250623E-546.359414
GACCCTA253.250623E-546.359416
TGACCGT253.250623E-546.359415
TTGACTC301.7544535E-646.359412
TACCTCA301.7544535E-646.359412
GACGGTG253.250623E-546.359415
CGGTGCG359.519317E-846.359415
CCCTTAC359.519317E-846.359413
TGCTCGG359.519317E-846.359411
GACATTC301.7544535E-646.359416
Produced by FastQC (version 0.11.5)