FastQCFastQC Report
Sat 31 Dec 2022
SRR5844137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154621
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC6100.3945130350987253No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5150.33307248045220245TruSeq Adapter, Index 2 (95% over 22bp)
GAAGCGGGTACTTAAGGTAGGCTCGGGAGAAAAAAATACAAAACTAATCATA2610.16879983960781522No Hit
GACTCAGGGGGTAGAAACAATACTTTTAAAGTGCTAAAAGAAAAGAGCTGTG2210.1429301323882267No Hit
AAAAGAGGGATGCAATGAAAGTGCCGGTGAGAATTTAGACAGCAAAGCTTAC2180.14098990434675754No Hit
TAAAGTGGGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAA2120.13710944826381927No Hit
TCTCAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC2070.1338757348613707No Hit
TTGCTAGGGGAGAATACAGACAGTAGGATCTGCATCCTTACTAGCATATAGA2010.12999527877843242No Hit
GTTGCTGGGCAACCCTGGGTGTGACTGACTTCTTAGATTTTATCAATGAGAT2000.12934853609794272No Hit
CCTACCGGGTTGGAAAGAAAATCCTAGAAAGCCTATCACCATAGATCAGCTA1990.128701793417453No Hit
GCGCTTAGGGACTCTTAACTCCTTGAGGGCACACAATTTTACTTATTGGTCC1990.128701793417453No Hit
CAACAAGGGATGAATGAAGCAGGTGTTGTAGAAGGTCAGTCAGTTAGACCAT1970.12740830805647357No Hit
TATGTCGGGACTGTGCTCGTTTTGTTGAATTGGTACATCAACAGAAAACTCC1930.12482133733451471No Hit
CACCACGGGAGATAATCTTGTGCTTCCAGATTGTTGGAATGAAAAACAAGCA1920.12417459465402501No Hit
ACAGATGGGTACAGGCGCGAGCCACTGTACCTGGCTAGTTGTGGTAGGATAT1910.12352785197353529No Hit
TGTTACGGGCATCCCAGCCCCCGGTTGGAGCCTGCTCTTTGAACTCCGCTGC1900.12288110929304556No Hit
AACTCTGGGATTGAAACTTCCCCCAAATCTGATGGACCTAGAAGTCTGCTTT1900.12288110929304556No Hit
ACGGCCGGGAGATAGGAATTGTTCGCCAGTTCCCATTTTCTTCTGCTTTGCA1900.12288110929304556No Hit
GCTAGCGGGATTTTTTCAACTCTGAAAATAACAGGAAACATCCAATTTTGCC1890.12223436661255586No Hit
GTCGATGGGGCTCAGTATTAGTAGACAAGGTCTTTGTTCAGACGATTAGGTG1890.12223436661255586No Hit
CTTGCAGGGCCCGGACGGAGGTAGAGGCCAGGGCAGCGCGTCCGGGAGCGGA1890.12223436661255586No Hit
TGTTAAGGGTATGAAGGAAGACCATGAATCATAAGTGCAAGAACTCCAGACA1850.119647395890597No Hit
ACTCTGGGCGATCTGCCTAGGGGAGGGGCATCAACTCCAGCTGGATTATTTT1850.119647395890597No Hit
TATGTGGGGAAACCCTTACTAGTGATTGTCCCTGAGGAGAGAACTTGTGGCT1840.1190006532101073No Hit
AGTAAGGGCTCTAGGAAATTATTGGCCTGCTTTCTGTCACTATCAATTAGCC1830.11835391052961758No Hit
TGGCCTTGGGCATCAAATAAAGCAAAACCTTGCAATATCAGCTAGATTTACA1810.11706042516863815No Hit
GACATGGGGCAACCAGGAAGACTACAGGCTTTTATCTGACTCTGGCCACACT1810.11706042516863815No Hit
AATTAGGGGATCGCTTTCCTTCTTGTTTGAATATATTGGTGAAATGGGAAAA1810.11706042516863815No Hit
TCGCTGGGCTGCATAATGAATTAACTAGAAACAACTTTGCAAGGAGAGCCAA1800.11641368248814844No Hit
GCTATTGGGCATTTGGAGCAATGTGATCAGTTTGCATTTAAAAGGAAAAAAA1800.11641368248814844No Hit
AGTTCCGGGTACAAAAAATACAAAAATTAGCCAGGCATGGTGGTGCACCTAT1800.11641368248814844No Hit
TCTCTAGGGGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAA1790.11576693980765872No Hit
ACTGTAGGGGATGCTCCATCCAGCTACTACTTTGGGGTTTATCTGGGAGTAG1780.11512019712716902No Hit
CGCTTGGGTAGGGGATGATCCAGGTCTTGGGATTTTCAAAGGTCCCCATGTG1780.11512019712716902No Hit
TAACATGGATGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAAC1770.11447345444667929No Hit
TGTATCGGGGAATGTTTTTATTTATAATGAGCATACCAGTGTGTAGCCTCTT1770.11447345444667929No Hit
TAGCAGGGATAAGGAGAAAGTCACCAACAGCTTGATCTATTATTTTTCCCTC1760.1138267117661896No Hit
CTATCATCGGGTAGAAACTAATGTGAAATCCACAAAACTACTATTAATTTTT1760.1138267117661896No Hit
TGCTAGCGGGATAGCAAAAGCTCAACAAATACTTGATGAATGAATAATTCCA1750.11317996908569987No Hit
GTGGCTAGGGGAGTCTTGGCGGAAGCCTGAGACGCAATAGATGGAGACTCTC1750.11317996908569987No Hit
GCTCTTGGGAGGTTGTCCCTAATTTGTCAGAGGCTGTAGTACCATATTTTCT1740.11253322640521016No Hit
ACTCTGGGGATGATGATATGGCTGCAGCCATGAAGGCAGTCACAGAACAGGG1740.11253322640521016No Hit
ACTCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1740.11253322640521016No Hit
TCATACGGGGTGCTCGGTGCGCTGAGCTCCGCGGCTCCGCGAGCCGGTTCCG1740.11253322640521016No Hit
GACCATGGGACACACGTGTGCTCCCTGCCCTGCTCCTGGCCCCTTGGCCGGC1740.11253322640521016No Hit
CACTGTGGTATAGATTCCATAATATGCTTGAATATTATGATATAGCCATTTA1730.11188648372472046No Hit
CGTGATGGGGAGAGCGAGCGCGGGAGGTCGCCGCAGCCAGGGACACCGCGCG1720.11123974104423073No Hit
GGTGGGGATGTGTGAATTTGATCCTGTCATTATGATGCTAGCTGGTTATTTT1720.11123974104423073No Hit
TTGTCAGGGACGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATAT1710.11059299836374101No Hit
TAGCTAGGGGGGGAATGAAGTGAGGCCAGTGGCCGGGTCTGCTGAGTCCGCA1710.11059299836374101No Hit
TAGACTGGGCCAATGAGTGGCAGAACCAAGACCGAAAGTCTGAGTTTTCAAG1710.11059299836374101No Hit
TTGAGTGGGAACTCCCCCTGGATGAGGCCCAGAGACGAAAGCTGGATGTCTC1700.10994625568325131No Hit
GTAAAAGGGGAATGGATGTTGCGGCAGCAAGGAAATTAGACAAGGAGCACAG1700.10994625568325131No Hit
AGCTAGGGGATTTGGAGAATAAATAAGAAGCTATGATTCTTTTTTAATAATT1690.10929951300276158No Hit
TCATGAGGGACTTGCTTACATAAACAGAAATTTAACAGCCCATATTCTTGAA1690.10929951300276158No Hit
ATCAACGGGGATCTACGCCGGGGGAAGGGTGGTAGGGAGGAAGTAATATAAT1690.10929951300276158No Hit
ATCTATGGGAACCAAATTTTCAAACCAGGCAACCTGCTTTCAGAGTTTGAAC1680.10865277032227189No Hit
GAAAGGGGGGTTTTATAAAGTAGTGCTTGCCGGGCATGCTGGCTCACTCCTG1680.10865277032227189No Hit
TTGTAAGGCAAAGAAGACTACAACACCAAAAGGTAAAGGCCGAGGGGCAAAG1680.10865277032227189No Hit
TGCTTGGGGATGTTTCTCTGGGGGCTGAAAGAGTGACTCATGTCTGGGAATG1670.10800602764178216No Hit
GCATTGGGAATGTTTACTTTCCTTGTCCTGTATTATTATATCTCTCAGGATT1670.10800602764178216No Hit
TGATTGGGGATGTCATCGGTATTGGAGCCCTGAGAAGAAAGAGATGTTTTCA1670.10800602764178216No Hit
GCTAGGGGGGACAATCTCTTCAATAAATGGTGCTGGAAAACTGGATATCCAT1670.10800602764178216No Hit
TGGGTTGGGCTTCCGATCTTGATAAGGGCAAGAATTGTTGTGTAACTCAAAG1670.10800602764178216No Hit
CGCTTGGGGGGACTCGCTGTTGGCCTTGGGCGGCCTGGTGCTGCTTCGGGAT1670.10800602764178216No Hit
CAGGCTGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1660.10735928496129245No Hit
TTGTATGGGATATTTAAAGAAAATAAAAGTTTGTTTTATATTTTAGTTTTTG1660.10735928496129245No Hit
GCGCTTGGGACCTGTGGTCTAAACCCCAAGTCATTATGTCTTTCTCAAGCTG1660.10735928496129245No Hit
AGATGTGGGTGGGGAATCTGTTTTATGAAAAACTCAGAAATATAAGTACGGA1660.10735928496129245No Hit
TACACGAGGGATTGCTAAGTATTCTTAAACATCGACTTCAGAAATCAAAATT1660.10735928496129245No Hit
AACTCAGGGGGAGGCAGAGGGTGCAGTGAGCTGAGATCGCACCACTTCACTC1660.10735928496129245No Hit
CTGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.10735928496129245No Hit
TCTGCTGGGAGAGGGCAGGTGTTTGTTTGCCAGTGGCAGTCCATTTGGGCCA1650.10671254228080275No Hit
GACTCTGGGGACGCTCTTCCGATCTAATCATGGGAGATTCATGAGTAGGTAA1640.10606579960031302No Hit
TACTGGGGGAAGTTAAAGGTGTTTTCCTTCCTCTACCATATACAATTTTTAA1640.10606579960031302No Hit
TTGCTGGGGACACTCTGCCTTTTTAAAAACACCTTTTGATAGAAAACTTTTT1630.1054190569198233No Hit
CATTCTGGGCAGTAGCCCAGGATTGCTACAAAAAGGTCCTTTGAGAAAATTA1630.1054190569198233No Hit
CACACTGGGTTGTTATTTTTATTTTTACAGATGAAGAAACAGCTCAGATTTG1630.1054190569198233No Hit
TCGTTGCGGGGGGCACAGGGCCAGTGAGGCCGGCCACGCTCGGGCCCTCACC1630.1054190569198233No Hit
GTCCAAGGGCAACCATGCCCAGCTATTTTTTGTTGTTGTTGGTTTTTTGTAG1620.1047723142393336No Hit
GCTCCAGGCAACTGAGCCCTACTCGCAGGTCCTCGGCCTTGGCCCAAACAGG1620.1047723142393336No Hit
AAACCGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.10412557155884389No Hit
TCATTTGGGATACTGGAGCTCATAACCCCTACTCCACAGCTGTGAGTACCTC1610.10412557155884389No Hit
GACATGGGGGGCGAGGGAAAGGTTTCAGGGTCCATCGAATTGAGTGAGAAGA1610.10412557155884389No Hit
GTCAAGGGGGATTCCTTTCCGTGTCAATGATGGCTTTTAATCCCAATTTCCA1610.10412557155884389No Hit
TGGTTTGGGATGTTTTTTGGCCATTTGTTGTGTAGCTTCTTTGAATAAATGC1610.10412557155884389No Hit
ATTAGAGGGGTTCCACATTAAATGCATTTTCATTATAAAAACTTGAAAAAAT1610.10412557155884389No Hit
TGACCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1610.10412557155884389No Hit
TGTAAGGGACAACTGGTGCTCAAGATGACTTGAGAAAAGTAACTCAGAGTGC1610.10412557155884389No Hit
TGCTGGGGGGGGCGATATATACAGCCTGGATGTGCTGGACAAAGGGATGATT1600.10347882887835416No Hit
CACACAGGGATCAAAAATAGAAAATTCATTTTATATTTCTAGCTGAAAATAA1600.10347882887835416No Hit
GCAGCAGGGGGAGTTAAAAGACAATTGAATAAAAACAGTAATCATATAAGTT1600.10347882887835416No Hit
CAACAAGGGCTCTCATGATTTTGATTTGTTTTTTTTCTTTTCAGTGAAACTA1600.10347882887835416No Hit
GCCTGGGCGACGGAGTGAGACTCCATCTCAAAAAAAAGAAAGGAAAAGAAAG1600.10347882887835416No Hit
GATTTAGGGGGGAAACAGCGCGGGGTCCGCTATGGCGGCGGCAGCCGAGGGC1600.10347882887835416No Hit
TCAAAAGGGCTTCGATAATGTCTATTGTTCCCTTGAGAGTCTTTTGATATTT1590.10283208619786446No Hit
TTTCGAGGGGGGAAGATGAAAGCAATTTACCAGAGGAACTCTCCCTTGTCTT1590.10283208619786446No Hit
TATCTAGGGGGTAAAGGTTCTTAGATTAACTCTGCATTTAACTTTATTTGCA1590.10283208619786446No Hit
CGACATGCGGGTGATGTTCTTCTCAAACTTCAAAAGTGTGTGTGTGTTTTAA1590.10283208619786446No Hit
TTTACAGGGGAATAGTATTTTTATAGCTTGTTAAAGACATGGCTAGTTGCAT1590.10283208619786446No Hit
TCACCTGGGGAGAAGCCTGGGTATGACTTTCCGTTGTGTACACTCACACTTC1590.10283208619786446No Hit
CTGTACGGGCAACATCTCCCTTTACAAAGTTAAAAATTTTGACCCTGACTCT1590.10283208619786446No Hit
GTGTGTGGGAACTTACCACAACCTGTTTCCTGATTCTATGACAGCAACACAG1580.10218534351737474No Hit
TACAATGGGACCAAAATTAAGTCCAAAGAACATATGTTTAGACCAACTTCAT1580.10218534351737474No Hit
GTAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1580.10218534351737474No Hit
AAAAGCGGGACTTCACACTGAGACACTTGGACGTCACATGTGAAGCCTACGA1580.10218534351737474No Hit
CAGCTCCACTTTGCAATCTTGTATCAAAGAGAGAGCATGCTGTCAGTTTCTG1570.10153860083688504No Hit
TTTACAAGGGCTAAGCATTCCTATTTTTTCCTAATTTCCTTATTTGTATTGC1570.10153860083688504No Hit
AGCTCTGGAAATACCTCAAAGCTGCTCCCTGCAGGCCACTCTGTATCAGGCT1570.10153860083688504No Hit
TTGGCAGGGGGTGCTTGGGAACTGCTGGTTATGAGCATTCCTGGTTTTCTTC1560.10089185815639531No Hit
GTCAGGGTGATTAAAAACATGAACCAACTGTATGCTATCTACAAGAAACATC1560.10089185815639531No Hit
TCTGCTGGGAGTCTTTTGCAGAGCAAAAGGTTTTAATTTTGTTAGAGTCGAA1550.10024511547590559No Hit
TTTGATGGGCCGATCTTAAGCCGGGAAAAGTAAAAAACTTCCCAACTCACTT1550.10024511547590559No Hit
CAGTGGGGTAAACATTCTCTAAGCACCAAATTTGGTAGGGATTTCCACTTCC1550.10024511547590559No Hit
TAGCATGGGATATAATTTGTATGAATTTATAATTCACGAAAATCTCAAAAAT1550.10024511547590559No Hit
GCGCTGGGGGTGATGTTCCTCCTTCTATGTCAGGAAGCTGTGCTGTGTGTGT1550.10024511547590559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)