FastQCFastQC Report
Sat 31 Dec 2022
SRR5844136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145609
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT10300.7073738573851891TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9300.6386967838526465No Hit
GATACAGGGTATTGGTAGATTTAGGTGTTTCTCATTTTTGCTGTTCTAAATA1750.1201848786819496No Hit
CTCCAACGGGTGTAGGGAAAAGAGCAATGCTCTTCTTGTGATGTTTCATTCT1720.11812456647597332No Hit
AGCCACGGGGTTTCAAGGCTTTTAATCTTGCAGTGAAAATTATAACTACAGG1680.11537748353467163No Hit
TATATAGGGAGGATAAAAGTCTAGTTTTACCTTTCATAATCCCCACATCCTA1600.10988331765206821No Hit
CTAAGTGGGCTTTGGGGACCACGCGAAAACCTCCAAGATATTTTTCACAAAA1530.10507592250479023No Hit
GTATGCGGGGTTCATAGAATTACAGAACTAGTGACATGGAAAGGACCTTAGA1490.10232883956348851No Hit
CATCCAGGGGCCTTGCCCTGATTCTAAAACCAGAGAAGGACACACAACAAAA1490.10232883956348851No Hit
CATAAGGGGGGGATTAAATTTCTAGATGAGATTTGGTTGGGGACACAGAGCC1490.10232883956348851No Hit
ATGCTGGGATTACAGGTGTGATCCACTGCACCCGGCCGGCATTATGATTTTG1470.10095529809283767No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)