FastQCFastQC Report
Sat 31 Dec 2022
SRR5844135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146283
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT9800.6699343054216826TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9610.6569457831737112No Hit
AGCCACGGGGTTTCAAGGCTTTTAATCTTGCAGTGAAAATTATAACTACAGG1850.12646719030919518No Hit
CTCCAACGGGTGTAGGGAAAAGAGCAATGCTCTTCTTGTGATGTTTCATTCT1710.11689670023174259No Hit
GTCGGCGGGCGAATACATTTCTGCACTCACATCCCCTTTCAGAGTGTTTCTT1640.11211145519301627No Hit
TAGTTTGGGTTCTGTAAGAATCTTAAATGTTCCTTTTCAAATTAGATGTGTT1640.11211145519301627No Hit
CATCCAGGGGCCTTGCCCTGATTCTAAAACCAGAGAAGGACACACAACAAAA1550.10595899728608246No Hit
TATATAGGGAGGATAAAAGTCTAGTTTTACCTTTCATAATCCCCACATCCTA1510.10322457154966742No Hit
GATACAGGGTATTGGTAGATTTAGGTGTTTCTCATTTTTGCTGTTCTAAATA1490.10185735868145991No Hit
GTTACGGGGAAAAATGGAATGGAAGATGCAGGGGACACAGAAGAGTTTCAGA1480.10117375224735616No Hit
TGATAAGGGACTTCATGACATCACAATTCTCCCCCTCTTGCTGTTCCCAGAT1470.1004901458132524No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)