FastQCFastQC Report
Sat 31 Dec 2022
SRR5844134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142181
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT9690.6815256609532919TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC8580.6034561579957941No Hit
AGCCACGGGGTTTCAAGGCTTTTAATCTTGCAGTGAAAATTATAACTACAGG1680.11815924771945618No Hit
CTCCAACGGGTGTAGGGAAAAGAGCAATGCTCTTCTTGTGATGTTTCATTCT1550.1090159725983078No Hit
TGATAAGGGACTTCATGACATCACAATTCTCCCCCTCTTGCTGTTCCCAGAT1490.10479599946547007No Hit
TCCACAGGGATTAAAGATTAGTTTCAAGTTCACATTCGCTATTCAGTTGTAA1480.10409267060999712No Hit
CATCCAGGGGCCTTGCCCTGATTCTAAAACCAGAGAAGGACACACAACAAAA1470.10338934175452416No Hit
CCATACGGGGTTATTTTCCTTTGCTAAATGGAAAGTCAAAATAAATAATATC1470.10338934175452416No Hit
ATGCTGGGATTACAGGTGTGATCCACTGCACCCGGCCGGCATTATGATTTTG1470.10338934175452416No Hit
AAATGGGGGAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTAT1470.10338934175452416No Hit
GTCGGCGGGCGAATACATTTCTGCACTCACATCCCCTTTCAGAGTGTTTCTT1470.10338934175452416No Hit
CGCTCAGGGATACTTCTTGCATGGAAGAGCAAAACAAATTCAAGTTGTCATG1470.10338934175452416No Hit
CTCTCCGGGCGATCTGGATAAGGGCCTTACATTTGAATAATAATTTTATAGG1460.10268601289905122No Hit
CTAAGTGGGCTTTGGGGACCACGCGAAAACCTCCAAGATATTTTTCACAAAA1440.10127935518810531No Hit
GAGATTGGGATCGAAGACAAAACTTTCGGTTTGAAGAATAAGAAAGGAGCAA1440.10127935518810531No Hit
CGTGCTGGGATTTAAAAAGAGCCTAGTCAGAGTTTTATCATATTCAAGAAAT1430.10057602633263235No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)