FastQCFastQC Report
Sat 31 Dec 2022
SRR5844133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21840
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5642.582417582417582No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5192.3763736263736264TruSeq Adapter, Index 2 (95% over 22bp)
CCAGCGGGACTAAATAACCAGCTGGAAACCAGATTATCAATAAAAGACATAA2621.1996336996336998No Hit
ACTGCAGGGAGAATTGATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG2401.098901098901099No Hit
ACAGACGGGGCTACCGGAGGGAGGAGGGAGGATGCGCTGTGGGGTTGTTTTT2321.0622710622710623No Hit
GAACAAGGGTAGTCCAACTGTTACAACCACTCTAAAAAACCTTGGGGCACTT2301.0531135531135531No Hit
CTAGGATGGGCTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA2110.9661172161172161No Hit
GCCACTGGGATGTTACTGCAATGGCATGAAAAGGCACTTGCTGCTGGAGGAG2050.9386446886446886No Hit
TAGCAAGGGCTGGTTTAGGGTAGGTTGGGCGCTCGATATCCAGGTTTCCACA2020.924908424908425No Hit
TTCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2000.9157509157509158No Hit
CGCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1910.8745421245421245No Hit
TGTGGAGGGAAACAAAACACATCCCAGACATTAGGAGTTCATAAGTATATTT1820.8333333333333334No Hit
TGCTTAGGGGAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAGCGGGTTCT1750.8012820512820512No Hit
CAAGGCGGGTCTGCAGCCAGCAAGCTGGGACTGGCAGGAAATAACCCACAAA1570.7188644688644689No Hit
AGTAAAGGGAATTTTATCAAACCTAACTGTGACAGCTGTGATTTGACACGTT1560.7142857142857143No Hit
TAGCCTGGGGTGATTCTCCCTGTTTTTTCCCTGTGTTTATATCACCAACAGT1500.6868131868131868No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGT1130.5173992673992673No Hit
TCACTTGGGAGTGCTGGTGTGTTGACAGATATGGAAATGAAGTCATGGGATC1130.5173992673992673No Hit
ATAAAGGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATA1040.4761904761904762No Hit
AAGCCTGGGCATAAAATTAGCTGTGGTACATACTGTACTACTGTAATAATTT1030.4716117216117216No Hit
TCTCTTGGGCTAAAAAACTTTGGAAATGGAAAATAATGTATTAGTGCAACAG940.43040293040293043No Hit
CAGTTAGGGGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC930.4258241758241758No Hit
CTCTAGGCGGGGCTTTGGAGGATTTGTATTGAGCTTTTACAGTATTCATTTT810.37087912087912084No Hit
TGTTGCGGGAGCTTCACTGGTGCAGAAAATTTTCCTCTAGATCAGAATCTTC690.3159340659340659No Hit
GCTGATGGGATACCAGAATGTACCAAAAAGTGCACAAGGTAAAGGTAATTTA640.29304029304029305No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTA620.2838827838827839No Hit
GTAAAGGGGATTTAAACTATAATAATAATAAAACTGGTCATTAGAGTACTTT610.2793040293040293No Hit
TTCATAGGGATTTGAAACAAATGAAAAACAGTCTCAGCAAAGAAATGAAAGA610.2793040293040293No Hit
TGTTTCGGGAATTTTAACATTCACAAATGTAAGTATTATAAATTAAGATAAA600.27472527472527475No Hit
CTTATAGGGATTGTGAACGTACTAAATGTCATAAAGTTGTTCATTTTAAAAT490.22435897435897434No Hit
CTTTAGGGGGTAAGTACATAAGGGCATGTTGATTTTGATATATTGATTGATT450.20604395604395606No Hit
GCCAAAGGGAGGGCGCGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGG440.2014652014652015No Hit
AACCAGGGGACAGTGACCTGACTGGGGGTGAGGGAGAAGGAGGAGAGAGCCC440.2014652014652015No Hit
GGTGAGGGGATACACCAAGGGTAAAAGCTTTCCCATAATTCAAAGTAATTTT420.19230769230769232No Hit
GCTTGCGGGCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAGCCGT400.18315018315018314No Hit
TAAAACGGGGGTACTGTAACTGAGCCCTTTCCCACATGTCTAGGCTCCAATG390.17857142857142858No Hit
CAATGAGGGGTACGTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT360.16483516483516483No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTAT350.16025641025641024No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCT340.15567765567765568No Hit
CGTTTAGGGATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCA340.15567765567765568No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTAT300.13736263736263737No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCTTATAC280.1282051282051282No Hit
CCAGACACAAAGTAGGAAACATCAAATCCTACCCTAATACAGGCAGAAGGAT260.11904761904761905No Hit
AGCAGAGGGCGATCTAGCTCCGGGTACATGGGCTGTATGTTATATAAGAGAA260.11904761904761905No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCC250.11446886446886448No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGT230.10531135531135531No Hit
CTATTAGGGGAGAGAGTAACATGGAAACTTATATTACCATATGTAAAATAGA220.10073260073260075No Hit
AGTCCTGGGTACTGCTTTTGAAAGAAGAAAATCAACAGCCCCTGCCTCTATT220.10073260073260075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)