FastQCFastQC Report
Sat 31 Dec 2022
SRR5844132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21816
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5792.6540154015401543No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT4652.131463146314631TruSeq Adapter, Index 2 (95% over 22bp)
CTAGGATGGGCTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA2251.0313531353135312No Hit
ACAGACGGGGCTACCGGAGGGAGGAGGGAGGATGCGCTGTGGGGTTGTTTTT2251.0313531353135312No Hit
TTCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2231.0221855518885221No Hit
ACTGCAGGGAGAATTGATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG2201.0084341767510085No Hit
TAGCAAGGGCTGGTTTAGGGTAGGTTGGGCGCTCGATATCCAGGTTTCCACA2120.9717638430509717No Hit
CCAGCGGGACTAAATAACCAGCTGGAAACCAGATTATCAATAAAAGACATAA2080.9534286762009535No Hit
GCCACTGGGATGTTACTGCAATGGCATGAAAAGGCACTTGCTGCTGGAGGAG2000.9167583425009167No Hit
TGTGGAGGGAAACAAAACACATCCCAGACATTAGGAGTTCATAAGTATATTT1960.8984231756508985No Hit
GAACAAGGGTAGTCCAACTGTTACAACCACTCTAAAAAACCTTGGGGCACTT1880.8617528419508618No Hit
CGCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1750.8021635496883022No Hit
TCACTTGGGAGTGCTGGTGTGTTGACAGATATGGAAATGAAGTCATGGGATC1680.77007700770077No Hit
CAAGGCGGGTCTGCAGCCAGCAAGCTGGGACTGGCAGGAAATAACCCACAAA1580.7242390905757242No Hit
AGTAAAGGGAATTTTATCAAACCTAACTGTGACAGCTGTGATTTGACACGTT1510.6921525485881922No Hit
TAGCCTGGGGTGATTCTCCCTGTTTTTTCCCTGTGTTTATATCACCAACAGT1500.6875687568756876No Hit
TGCTTAGGGGAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAGCGGGTTCT1420.6508984231756508No Hit
ATAAAGGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATA1320.605060506050605No Hit
AAGCCTGGGCATAAAATTAGCTGTGGTACATACTGTACTACTGTAATAATTT1100.5042170883755043No Hit
CAGTTAGGGGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC1080.49504950495049505No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGT990.45379537953795385No Hit
TCTCTTGGGCTAAAAAACTTTGGAAATGGAAAATAATGTATTAGTGCAACAG940.4308764209754309No Hit
TGTTGCGGGAGCTTCACTGGTGCAGAAAATTTTCCTCTAGATCAGAATCTTC800.3667033370003667No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTA770.35295196186285294No Hit
TTCATAGGGATTTGAAACAAATGAAAAACAGTCTCAGCAAAGAAATGAAAGA760.3483681701503484No Hit
CTCTAGGCGGGGCTTTGGAGGATTTGTATTGAGCTTTTACAGTATTCATTTT700.32086541987532086No Hit
GCTGATGGGATACCAGAATGTACCAAAAAGTGCACAAGGTAAAGGTAATTTA660.3025302530253025No Hit
TGTTTCGGGAATTTTAACATTCACAAATGTAAGTATTATAAATTAAGATAAA580.26585991932526587No Hit
AACCAGGGGACAGTGACCTGACTGGGGGTGAGGGAGAAGGAGGAGAGAGCCC560.2566923359002567No Hit
GTAAAGGGGATTTAAACTATAATAATAATAAAACTGGTCATTAGAGTACTTT540.24752475247524752No Hit
CTTTAGGGGGTAAGTACATAAGGGCATGTTGATTTTGATATATTGATTGATT510.23377337733773376No Hit
GCCAAAGGGAGGGCGCGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGG480.22002200220022No Hit
GCTTGCGGGCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAGCCGT450.20627062706270624No Hit
CTTATAGGGATTGTGAACGTACTAAATGTCATAAAGTTGTTCATTTTAAAAT440.20168683535020168No Hit
TAAAACGGGGGTACTGTAACTGAGCCCTTTCCCACATGTCTAGGCTCCAATG430.1971030436376971No Hit
GGTGAGGGGATACACCAAGGGTAAAAGCTTTCCCATAATTCAAAGTAATTTT360.16501650165016502No Hit
CGTTTAGGGATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCA340.15584891822515584No Hit
CAATGAGGGGTACGTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT310.1420975430876421No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTAT290.13292995966263293No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGT280.12834616795012835No Hit
CCAGACACAAAGTAGGAAACATCAAATCCTACCCTAATACAGGCAGAAGGAT280.12834616795012835No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCTTATAC280.12834616795012835No Hit
CATATTGGGCGATCTTTACGGGGGACTCCCACTTCATCCTGGCTGAAAGCAG270.12376237623762376No Hit
AGCAGAGGGCGATCTAGCTCCGGGTACATGGGCTGTATGTTATATAAGAGAA260.11917858452511919No Hit
AAAGCAGGGACTCGGGGAATTCCAGGCCCACCAGGACACGTGCCAATGGCCC250.11459479281261459No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCT230.10542720938760543No Hit
ATTAAAGGGACATTCATAGAGACAGAAATTACGTCGGTGTTTACCAGGGCCT230.10542720938760543No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGT230.10542720938760543No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCC230.10542720938760543No Hit
AATTTGGGGAAGGAAAAGTACATCGATCAAGAAGAGCTCAACAAAACAAAGC230.10542720938760543No Hit
AATGTCGGGCTGCTTTTCCTGGCAGGCATTTGCCGGCCTATGTGCTTTAACC220.10084341767510084No Hit
ATATATGGGGCTAGGAAAGGTTTCTCCTATTTGGTAACTGGAGTAACTACTG220.10084341767510084No Hit
ATAGCTGGGGGGAAGCTGAGGTGGGCGGATCACTTGAGGTCAGGAGTTTGAG220.10084341767510084No Hit
ACTTAAGGGCTGAGTTCTAGTTTCATCATTTATAATGGATAAAAATAATAGT220.10084341767510084No Hit
GATAGGGGGGTGCTGGTTTGCGTCGTAGTCTCCTGCAGCGTCTGGGGTTTCC220.10084341767510084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)