FastQCFastQC Report
Sat 31 Dec 2022
SRR5844131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21316
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC5212.44417339088009No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT5052.3691124038281104TruSeq Adapter, Index 2 (95% over 22bp)
CCAGCGGGACTAAATAACCAGCTGGAAACCAGATTATCAATAAAAGACATAA2351.1024582473259523No Hit
ACAGACGGGGCTACCGGAGGGAGGAGGGAGGATGCGCTGTGGGGTTGTTTTT2281.0696190654907112No Hit
ACTGCAGGGAGAATTGATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG2161.0133233252017264No Hit
CTAGGATGGGCTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA2120.9945580784387315No Hit
TAGCAAGGGCTGGTTTAGGGTAGGTTGGGCGCTCGATATCCAGGTTTCCACA2070.9711015199849878No Hit
GAACAAGGGTAGTCCAACTGTTACAACCACTCTAAAAAACCTTGGGGCACTT2050.9617188966034904No Hit
TGTGGAGGGAAACAAAACACATCCCAGACATTAGGAGTTCATAAGTATATTT1840.8632013510977669No Hit
GCCACTGGGATGTTACTGCAATGGCATGAAAAGGCACTTGCTGCTGGAGGAG1790.8397447926440232No Hit
TGCTTAGGGGAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAGCGGGTTCT1620.7599924939012949No Hit
AGTAAAGGGAATTTTATCAAACCTAACTGTGACAGCTGTGATTTGACACGTT1580.7412272471382999No Hit
TTCCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1520.7130793769938075No Hit
CAAGGCGGGTCTGCAGCCAGCAAGCTGGGACTGGCAGGAAATAACCCACAAA1470.6896228185400638No Hit
CGCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1380.6474010133233252No Hit
ATAAAGGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATA1350.633327078251079No Hit
TAGCCTGGGGTGATTCTCCCTGTTTTTTCCCTGTGTTTATATCACCAACAGT1340.6286357665603303No Hit
TCTCTTGGGCTAAAAAACTTTGGAAATGGAAAATAATGTATTAGTGCAACAG1210.5676487145805967No Hit
TCACTTGGGAGTGCTGGTGTGTTGACAGATATGGAAATGAAGTCATGGGATC1130.5301182210546069No Hit
CAGTTAGGGGGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGC1070.5019703509101144No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGT1030.4832051041471195No Hit
AAGCCTGGGCATAAAATTAGCTGTGGTACATACTGTACTACTGTAATAATTT950.4456746106211297No Hit
CTCTAGGCGGGGCTTTGGAGGATTTGTATTGAGCTTTTACAGTATTCATTTT850.39876149371364233No Hit
TGTTGCGGGAGCTTCACTGGTGCAGAAAATTTTCCTCTAGATCAGAATCTTC750.351848376806155No Hit
GCTGATGGGATACCAGAATGTACCAAAAAGTGCACAAGGTAAAGGTAATTTA670.31431788328016513No Hit
GTAAAGGGGATTTAAACTATAATAATAATAAAACTGGTCATTAGAGTACTTT650.30493525989866765No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTA630.2955526365171702No Hit
GGTGAGGGGATACACCAAGGGTAAAAGCTTTCCCATAATTCAAAGTAATTTT620.29086132482642146No Hit
CTTTAGGGGGTAAGTACATAAGGGCATGTTGATTTTGATATATTGATTGATT570.2674047663726778No Hit
AACCAGGGGACAGTGACCTGACTGGGGGTGAGGGAGAAGGAGGAGAGAGCCC570.2674047663726778No Hit
TTCATAGGGATTTGAAACAAATGAAAAACAGTCTCAGCAAAGAAATGAAAGA570.2674047663726778No Hit
TGTTTCGGGAATTTTAACATTCACAAATGTAAGTATTATAAATTAAGATAAA540.2533308313004316No Hit
GCCAAAGGGAGGGCGCGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGG520.24394820791893415No Hit
GCTTGCGGGCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAGCCGT470.22049164946519048No Hit
CTTATAGGGATTGTGAACGTACTAAATGTCATAAAGTTGTTCATTTTAAAAT470.22049164946519048No Hit
CAATGAGGGGTACGTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT410.19234377932069807No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTAACTCGTATGC370.17357853255770314No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCT360.1688872208669544No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGT350.16419590917620566No Hit
CGTTTAGGGATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCA350.16419590917620566No Hit
TAAAACGGGGGTACTGTAACTGAGCCCTTTCCCACATGTCTAGGCTCCAATG340.15950459748545692No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGT300.140739350722462No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCCTGTCTCTTATAC290.13604803903171325No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTAT280.1313567273409645No Hit
TGAACTGGGTGAAAAAGTCATGAATTACTAAAAACTCTGGGAATATAAACTA260.12197410395946708No Hit
AGCAGAGGGCGATCTAGCTCCGGGTACATGGGCTGTATGTTATATAAGAGAA260.12197410395946708No Hit
CATATTGGGCGATCTTTACGGGGGACTCCCACTTCATCCTGGCTGAAAGCAG250.11728279226871834No Hit
GATCTGCGGGAAGGCAGAAAAATAAAGATATTCTTAGAAACCAATGAGAACA230.10790016888722086No Hit
GGCCGCGGGCAAGAGGGCATAACCAAAGCTTAAATTTGTCAAGATTTCCTTT220.10320885719647213No Hit
AAAGCAGGGACTCGGGGAATTCCAGGCCCACCAGGACACGTGCCAATGGCCC220.10320885719647213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)