FastQCFastQC Report
Sat 31 Dec 2022
SRR5844127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212511
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT10510.4945626344048073TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9120.4291542555444189No Hit
TATAACGGGATATTGAAACTTAAAACTTAGCTGGTTTTTCTTCATATTTTTA3070.14446311014488664No Hit
GCGTAAGGGACAAGACTGAAAAATATTCTTTCTGTACTTTCATTTGAAATAA3050.14352198239149974No Hit
TTAGCCGGGGGCACAGAAAGATGAAGCTGTTGGCCAAAGGATGCCTTGTTCA2880.13552239648771122No Hit
TTACGCGGGGACAGGAGTATATATCATAAAGCAAACACAAGGGAATTTAAAA2840.13364014098093746No Hit
ACTGCAGGTTTTGGCCAGCCCTCCAGCACAACAGCAGGATGGTCTGGAAGCT2780.1308167577207768No Hit
CAGAAAGGGCACTGTGATAGAACCTGGACTGCTTTTTGAGATAATAGAGATG2680.1261111189538424No Hit
AGTTTAGGGCTGCACTAATTTTGACTTATGAAAAGATTCAGCAGTAAGAATA2680.1261111189538424No Hit
TGCCGGGAGAAGAAAATAAAATGGAGTGATAAGCAAAAGACAGTGGCCAGTC2660.1251699912004555No Hit
ACACCGGAGTGTAAATTGTGTCAACTGGACCCTGAAATAGAAAATTATTTCA2630.12375829957037518No Hit
ACTAACGGGATAGAAGGGGCATTACAAAAATTGAAGCTTGTGACATATTTAT2590.12187604406360142No Hit
GGATTAGGGGCGCGCTCCCGGGCCGAGCGCCGCGCGCCATGCTGGCCAAGTT2580.12140548018690799No Hit
TCTGCTGGGATTACAGGCGTAGCCACTATGCCCGGCTTGGCTCTCACTTTCG2570.12093491631021452No Hit
CATTACGGGGTGGTGAATAATATTTAAAAGTCCAAATGTTTACTTTTTCTTG2550.11999378855682763No Hit
GCGAGGGGGTGAGGCCGCCAGCAGCAATTTGTACCCTGCGTCATTTGGGTGG2540.1195232246801342No Hit
CTAACTGGGGATCTAAGCAAAGGCATGAAACAATTTAAATACACCCAGAATA2520.11858209692674732No Hit
ATGGTAGGGAGTTGGCTTCAGTTTGGAGATCTTCCATGCTCCTAAAATCAGC2520.11858209692674732No Hit
GGCAAGTTGTCCTGGGTTCTGTGAGTCCTGAGATGGATTTAATTCTTGATGC2520.11858209692674732No Hit
CTCCACAGGGTCACAAGTCATTTATGAGATGAAAGCCATATTTTAGGAACTA2510.11811153305005388No Hit
GCGATGGGATCTGCATACGGGCATATTGTACATGCAGTTTCACATTTTGCTT2500.11764096917336045No Hit
TACGCTGGGATATTTAAAATAGCTGATTTAAAATCTTTGTAAGGTAAGTTCA2500.11764096917336045No Hit
CCTAAGGGGAGCGCAGGAGTCCCCGGCGCACCCGCTGCGCTCGCCCCGCAGC2490.11717040529666699No Hit
GCAGTCCGGGCGATCTACCTAGGGGTGCTCCAGCCCAGCCGGGCTTCAGTTC2470.11622927754328012No Hit
TAGACTAGGGCAGGTTAAAATCTCTGTGAGGCAAAGGGATAATATTCTCTAG2470.11622927754328012No Hit
TAATGCGGGATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCTA2440.1148175859131998No Hit
TTATATGGGACCACACAAAAAATCACTTATATGGAGGTTATTCTCTTAGTAG2430.11434702203650633No Hit
TTGGTGGGGACTGCTAGGGGCTTAAGCGGAGGGAGTCGAGCCAGCGTCGCCG2430.11434702203650633No Hit
AATCCCGGGCTTAAAGATTGCAGTGAACTTACACCAAATTAGGCATTCATTG2410.11340589428311947No Hit
TAATTTGGGATATTGTGAAAGAAAGATGACAAGGAGTTTATTTTATAATATG2390.11246476652973258No Hit
AAAATGGGGACCAGAGTAAAAAATTACTTTTGATTGCCACACTTCTAAAATA2390.11246476652973258No Hit
AGTATGGGTCATTACTGCTGAAATGTCCTCACATCTCTTCCACTGTTCTTCA2380.11199420265303915No Hit
GAAACAGGGATCAGACCCAAGCACATTCTCCCAACATCAGCCAATGGAGAGA2380.11199420265303915No Hit
TAAAAGGGGAGGGAAACACCCTTGACATTCTGTATCTCCATCCCCTAGATCG2380.11199420265303915No Hit
GAGCATGGGAGAGTGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAA2370.11152363877634569No Hit
CACTATGGGTGATTTAAAAAAGAAAAGTGCTGAGTGGACGGAGTAATCAGAG2320.1091708193928785No Hit
ATGTATGGGGAACAACATGGGTTTGAACTGCATGGGCACACTTACATGCAGA2310.10870025551618503No Hit
GCTTTTGGGTAAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAATGA2300.1082296916394916No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2300.1082296916394916No Hit
CAATAGGGGACCTGAATAGTCTATTTATTGTAATTACATTTTTCTGAAATGG2290.10775912776279815No Hit
AATACCTGGGAGGGAATAATATGGACTCAAGAATGGAGCTTTATCAGTGGCA2290.10775912776279815No Hit
CTCTAACAGGGAGGATGGTACTGGCATAAAAACAGACACACAGACGAATAGA2280.10728856388610472No Hit
AGAGTTGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCAG2280.10728856388610472No Hit
GCTAAAGGGACTAATTAAATAATGTGTGGCATGGAAATAAATGAAATACTGT2280.10728856388610472No Hit
TACGCAGGGAAGTACTTCCGATGGAGGAACTGACTCACCTGGTACTGCGTCC2270.10681800000941129No Hit
CAATGAGGGAATATATAACAATTTAAAAATGGGTAAAAGACTTGGCGTGTTG2270.10681800000941129No Hit
TTCGTTGGGATTGGCCTGTGACTAATAAAGAATTTGTTGAAGAATGTTAACT2260.10634743613271783No Hit
ACAGCGGGAGAAGATCTTCTCTTTATTTGAGACTCAGAATTCCTTCTAGAAG2260.10634743613271783No Hit
GTAGCGAGGGAGATGTTCTGATAGAAGAATGTAAAGAATCCAGCAAAGGCAA2250.10587687225602439No Hit
TTTGCAGGGAACCAGGTAGGTGTGTTTTCTGTCCTTCTATGGTAATTGTATT2250.10587687225602439No Hit
CTCAACGGGATATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAG2240.10540630837933096No Hit
TTATGTGGGTATTCAGTAAATGTTACTGAGCATTCATTATGTGCCAGGCATC2220.10446518062594407No Hit
CATCCAGGGACTGTTACAGAGAAATATATTTGCATAAAATGGAAATTTTTGT2220.10446518062594407No Hit
ATCGTCGGGAGCAACTTCAAAGCTGCAAAAATAACTGGCTCTAACACGTACC2220.10446518062594407No Hit
TGTCAGGGGCATGCAGGGGAAATGATTCATATGTGGACACATGCTGATGGCA2220.10446518062594407No Hit
GCGAACGGACAGCTTTCTCCTTTCCATTGAACTGTAACCTCATTCAAAACTT2220.10446518062594407No Hit
ATTTATGGGTATGTTTAAAAAAGCAAGTAACTGGACATGAAACTTTTCTCAT2200.10352405287255717No Hit
TTCAGCGGGACTGTTTTATTTTTAGCATGTAGTATTGTAGATACAAATACGC2200.10352405287255717No Hit
CTTACAGGGACATTGTGGACCAGCAGAAATTTAATAGGGAAATCCTAGAAAT2200.10352405287255717No Hit
TTACATGGGGATGGCTCCGGGTTAATGCTAAAACAGTAGCAGAAAGTAGTCA2190.10305348899586374No Hit
AAAGCGGGGATAACACCTTCTGGTCAAAAACTCTTGCCTAATACCCTGACAG2170.10211236124247686No Hit
GAACTAGGGAACGATGTTGCCACCATCCTGTCTCGCCGTATTCCTGTTGAAT2160.10164179736578342No Hit
TTCAGGGGGATTGTTCAACATTTTTATTTCAAGGTGTATGACTACGATGCTG2150.10117123348908999No Hit
GGGTAATAATATACTATCGCATTGTCTCACTCAATAGTTTTATTATTTTATT2150.10117123348908999No Hit
TTTTTACGGGGCTAGTAAAACCAATGAATGTTTAACTCCTTCATAAGCAAAA2140.10070066961239653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)