FastQCFastQC Report
Sat 31 Dec 2022
SRR5844126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211990
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT11040.5207792820416057TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9030.4259634888438134No Hit
GCGTAAGGGACAAGACTGAAAAATATTCTTTCTGTACTTTCATTTGAAATAA3290.15519599981131185No Hit
AGTTTAGGGCTGCACTAATTTTGACTTATGAAAAGATTCAGCAGTAAGAATA2880.13585546488041889No Hit
TACGCTGGGATATTTAAAATAGCTGATTTAAAATCTTTGTAAGGTAAGTTCA2820.13302514269541016No Hit
TTAGCCGGGGGCACAGAAAGATGAAGCTGTTGGCCAAAGGATGCCTTGTTCA2710.12783621868956083No Hit
AAAATGGGGACCAGAGTAAAAAATTACTTTTGATTGCCACACTTCTAAAATA2700.1273644983253927No Hit
CAGAAAGGGCACTGTGATAGAACCTGGACTGCTTTTTGAGATAATAGAGATG2680.12642105759705646No Hit
CTCTAACAGGGAGGATGGTACTGGCATAAAAACAGACACACAGACGAATAGA2670.12594933723288834No Hit
TCCCTTGGGACCAGGCTGGTGAAGAAGCCAGACCAAGCCCGGGTTCGGAAAC2670.12594933723288834No Hit
TGCCGGGAGAAGAAAATAAAATGGAGTGATAAGCAAAAGACAGTGGCCAGTC2670.12594933723288834No Hit
AACGCCGGGAGTGCGTGATGACCCCAGAGCGTGCGTTCGGTGGCCCATAGGG2660.12547761686872022No Hit
CATCTTGGGAGGAAGGAACTAACTTGTTTTTGATTTTACAGGCTTATAGGCA2640.12453417614038398No Hit
TTGGTGGGGACTGCTAGGGGCTTAAGCGGAGGGAGTCGAGCCAGCGTCGCCG2620.12359073541204774No Hit
CATTACGGGGTGGTGAATAATATTTAAAAGTCCAAATGTTTACTTTTTCTTG2610.12311901504787962No Hit
CCTAAGGGGAGCGCAGGAGTCCCCGGCGCACCCGCTGCGCTCGCCCCGCAGC2600.1226472946837115No Hit
ACTGCAGGTTTTGGCCAGCCCTCCAGCACAACAGCAGGATGGTCTGGAAGCT2580.12170385395537525No Hit
GGATTAGGGGCGCGCTCCCGGGCCGAGCGCCGCGCGCCATGCTGGCCAAGTT2570.12123213359120713No Hit
TAAAAGGGGAGGGAAACACCCTTGACATTCTGTATCTCCATCCCCTAGATCG2570.12123213359120713No Hit
TTATATGGGACCACACAAAAAATCACTTATATGGAGGTTATTCTCTTAGTAG2540.11981697249870277No Hit
TATAACGGGATATTGAAACTTAAAACTTAGCTGGTTTTTCTTCATATTTTTA2520.11887353177036653No Hit
CTAACTGGGGATCTAAGCAAAGGCATGAAACAATTTAAATACACCCAGAATA2520.11887353177036653No Hit
TGACCAGGGTCTCTTAGTAAATAAATTAGTAACCTGCTTTCTAAAAATAACC2500.11793009104203028No Hit
ATGTATGGGGAACAACATGGGTTTGAACTGCATGGGCACACTTACATGCAGA2490.11745837067786216No Hit
ACACCGGAGTGTAAATTGTGTCAACTGGACCCTGAAATAGAAAATTATTTCA2480.11698665031369404No Hit
CACTATGGGTGATTTAAAAAAGAAAAGTGCTGAGTGGACGGAGTAATCAGAG2480.11698665031369404No Hit
TTACGCGGGGACAGGAGTATATATCATAAAGCAAACACAAGGGAATTTAAAA2470.11651492994952592No Hit
TTTAATGGGCACCAGATTATTTTTTCTTCAACTGTTTTGACTTTTATTGTTG2450.11557148922118968No Hit
CTTACAGGGACATTGTGGACCAGCAGAAATTTAATAGGGAAATCCTAGAAAT2440.11509976885702156No Hit
TAGTACTGGGCAATGTAAACAGAAGAAGTATACACCAATTCCAGGTAGAAAC2420.11415632812868531No Hit
TGAGGGGGGGTCCCTCAATGGATTGGATGGTGCCTGCTCACATTGGTGAGGG2410.11368460776451719No Hit
GTAGCGAGGGAGATGTTCTGATAGAAGAATGTAAAGAATCCAGCAAAGGCAA2390.11274116703618095No Hit
GCTAAAGGGACTAATTAAATAATGTGTGGCATGGAAATAAATGAAATACTGT2380.11226944667201283No Hit
TACGCTGGGATTTGCCTTTCTACTAAAAATGCTTTTATTCTGTCATTTCAAA2380.11226944667201283No Hit
TCAAACGGGTGTGAATGAACTGCTTTCAGAATAAGAACATTTATTTACAAGT2370.11179772630784471No Hit
TATGCAGGGTAATTTCCTTAAGCATGACAATGACTAAAGAGAAAATATTCAT2370.11179772630784471No Hit
TTCGTTGGGATTGGCCTGTGACTAATAAAGAATTTGTTGAAGAATGTTAACT2350.11085428557950847No Hit
ACAGCGGGAGAAGATCTTCTCTTTATTTGAGACTCAGAATTCCTTCTAGAAG2350.11085428557950847No Hit
ATTGGAGGGGAGACCAGCCTGACCAACATGGACATACCCCATCTCTACTAAA2340.11038256521534034No Hit
CTCCACAGGGTCACAAGTCATTTATGAGATGAAAGCCATATTTTAGGAACTA2340.11038256521534034No Hit
TCTGCTGGGATTACAGGCGTAGCCACTATGCCCGGCTTGGCTCTCACTTTCG2330.10991084485117222No Hit
GCGATGGGATCTGCATACGGGCATATTGTACATGCAGTTTCACATTTTGCTT2320.1094391244870041No Hit
GCGAGGGGGTGAGGCCGCCAGCAGCAATTTGTACCCTGCGTCATTTGGGTGG2320.1094391244870041No Hit
GAAAAAGGGGTTGTATCTGGAAAATCCTTGACAGAAGAGGCCTGATGAAAGG2300.10849568375866786No Hit
TAGCCTGGGATTGAAACCAGAAAACGTGAAAGTGTTAGGAGGTGAAGTAGAT2300.10849568375866786No Hit
AGAGTTGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCAG2300.10849568375866786No Hit
GCTTTTGGGTAAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAATGA2290.10802396339449974No Hit
AACAGCGGGACCTGCAAATGGCGCTTCCAGTACTGTGTTCAGTGATCCACAT2290.10802396339449974No Hit
ACAAGCGGGAGATGTAGGCATTTGCAAAAATAATTAATATACCATTGAATAT2280.10755224303033162No Hit
ACACACAGGGGATCTTTCTTGTTTTTGATGTAGGTGTTTATTACTAAATACT2280.10755224303033162No Hit
CAATGAGGGAATATATAACAATTTAAAAATGGGTAAAAGACTTGGCGTGTTG2280.10755224303033162No Hit
AAGCCAGGGGCTTATATGAAGAAGAAAGGTTTAAAATCAGTGACCTAAGCTG2270.1070805226661635No Hit
GAGCATGGGAGAGTGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAA2270.1070805226661635No Hit
TTCAGCGGGACTGTTTTATTTTTAGCATGTAGTATTGTAGATACAAATACGC2270.1070805226661635No Hit
TAGCATGGGCTTTGCAGGCATACAGCAACAGTGGAGCTCCTTTTGAAGGAAA2270.1070805226661635No Hit
TTAAAAGGGCAGCTTGCAAATTCTTGGACACAGCGCTTTCTTTTCCACCTGA2260.10660880230199538No Hit
AATCCCGGGCTTAAAGATTGCAGTGAACTTACACCAAATTAGGCATTCATTG2260.10660880230199538No Hit
ACTAACGGGATAGAAGGGGCATTACAAAAATTGAAGCTTGTGACATATTTAT2260.10660880230199538No Hit
GAAACAGGGATCAGACCCAAGCACATTCTCCCAACATCAGCCAATGGAGAGA2250.10613708193782725No Hit
GCAACAGGGCCTGACATCACTGTGGAAGTGGTATTTGAGTTGTGGATCTTTC2240.10566536157365913No Hit
CTGGCTGGGGGAGGCCGTGGAGCACAAAGCAGATTCTGATTGGGAGCAACCG2240.10566536157365913No Hit
TGTCAGGGGCATGCAGGGGAAATGATTCATATGTGGACACATGCTGATGGCA2240.10566536157365913No Hit
TTTTTACGGGGCTAGTAAAACCAATGAATGTTTAACTCCTTCATAAGCAAAA2230.10519364120949101No Hit
AGTACCGGGACTCCAGCAGGAAATCGGGTCAGGGGAGGGTGGTCCTTTTCTC2230.10519364120949101No Hit
ACGCCAGGCTATATTTTTTAAGGTAGTAATTTCATCACAAGAACCATTAAGA2230.10519364120949101No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2230.10519364120949101No Hit
ATTTATGGGTATGTTTAAAAAAGCAAGTAACTGGACATGAAACTTTTCTCAT2220.10472192084532289No Hit
TAATGCGGGATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCTA2220.10472192084532289No Hit
AAGTACGGGCAGTTCACAGCAGTGCCTCATCCCAACGTCAAGCCCATGGCCT2200.10377848011698665No Hit
AATTAAGGGCTTCTGAAAAGGGAGGAGTATTTTTAGTTTGACAATTTAATAA2200.10377848011698665No Hit
GAACTAGGGAACGATGTTGCCACCATCCTGTCTCGCCGTATTCCTGTTGAAT2190.10330675975281853No Hit
TAGCGAGGGTGAAAAAGCAAAATTTAACCTGTGCCTGAATGAGTAGAAGGTA2190.10330675975281853No Hit
ATGGTAGGGAGTTGGCTTCAGTTTGGAGATCTTCCATGCTCCTAAAATCAGC2180.1028350393886504No Hit
TTTGCAGGGAACCAGGTAGGTGTGTTTTCTGTCCTTCTATGGTAATTGTATT2180.1028350393886504No Hit
GCAGTCCGGGCGATCTACCTAGGGGTGCTCCAGCCCAGCCGGGCTTCAGTTC2170.10236331902448229No Hit
TAGCCTGGGGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAA2160.10189159866031416No Hit
CGTTAAGGGAGTTGGTCGAAGACAGAGGTTCAGGTAAGGATGACTGATAGGA2150.10141987829614604No Hit
AATCATGGGGGGCTAATTATTTGCTAGTCTCACTGTGTTTCAGAGTATCTGA2140.10094815793197792No Hit
TATCGAAGGGACTCGTTGAGACTTATAGTTCAGGGGAGGGAAATGGTAAACA2140.10094815793197792No Hit
ATTGGCGGGACAGAAAAATAAGGCCTTCATAAATGAGTAGAGAGCCACAGCA2130.1004764375678098No Hit
GCTATTGGGGGTATGAGAGGAGAGAGGTCAAGATGGAAAAAAGAAAGAAGCA2130.1004764375678098No Hit
AGCGATGGGTAAGCTGCCAATTGATAGGCATTATTCTTTTTAAATAGCCATT2120.10000471720364168No Hit
CTCAACGGGATATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAG2120.10000471720364168No Hit
TTAGCCGGGTACCTAGGTAACCTTCAATTTATTCTCTGTTGTTATATCTTAT2120.10000471720364168No Hit
TAGACTAGGGCAGGTTAAAATCTCTGTGAGGCAAAGGGATAATATTCTCTAG2120.10000471720364168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)