FastQCFastQC Report
Sat 31 Dec 2022
SRR5844125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209040
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT10870.5199961729812477TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9010.4310179869881362No Hit
GCGTAAGGGACAAGACTGAAAAATATTCTTTCTGTACTTTCATTTGAAATAA3510.16791044776119401No Hit
TATAACGGGATATTGAAACTTAAAACTTAGCTGGTTTTTCTTCATATTTTTA3170.15164561806352853No Hit
TACGCTGGGATATTTAAAATAGCTGATTTAAAATCTTTGTAAGGTAAGTTCA3010.14399158055874473No Hit
TTAGCCGGGGGCACAGAAAGATGAAGCTGTTGGCCAAAGGATGCCTTGTTCA2820.134902411021814No Hit
ACTAACGGGATAGAAGGGGCATTACAAAAATTGAAGCTTGTGACATATTTAT2790.13346727898966704No Hit
TGCCGGGAGAAGAAAATAAAATGGAGTGATAAGCAAAAGACAGTGGCCAGTC2650.12676999617298124No Hit
CAGAAAGGGCACTGTGATAGAACCTGGACTGCTTTTTGAGATAATAGAGATG2620.1253348641408343No Hit
AACGCCGGGAGTGCGTGATGACCCCAGAGCGTGCGTTCGGTGGCCCATAGGG2600.12437810945273632No Hit
CTCCACAGGGTCACAAGTCATTTATGAGATGAAAGCCATATTTTAGGAACTA2600.12437810945273632No Hit
CTAACTGGGGATCTAAGCAAAGGCATGAAACAATTTAAATACACCCAGAATA2570.12294297742058936No Hit
ACACCGGAGTGTAAATTGTGTCAACTGGACCCTGAAATAGAAAATTATTTCA2560.12246460007654038No Hit
ACTGCAGGTTTTGGCCAGCCCTCCAGCACAACAGCAGGATGGTCTGGAAGCT2510.12007271335629545No Hit
TTCGTTGGGATTGGCCTGTGACTAATAAAGAATTTGTTGAAGAATGTTAACT2490.11911595866819748No Hit
TTATATGGGACCACACAAAAAATCACTTATATGGAGGTTATTCTCTTAGTAG2490.11911595866819748No Hit
TAAAAGGGGAGGGAAACACCCTTGACATTCTGTATCTCCATCCCCTAGATCG2490.11911595866819748No Hit
AGTTTAGGGCTGCACTAATTTTGACTTATGAAAAGATTCAGCAGTAAGAATA2480.11863758132414849No Hit
CATTACGGGGTGGTGAATAATATTTAAAAGTCCAAATGTTTACTTTTTCTTG2470.11815920398009949No Hit
TTACGCGGGGACAGGAGTATATATCATAAAGCAAACACAAGGGAATTTAAAA2440.11672407194795253No Hit
GCGATGGGATCTGCATACGGGCATATTGTACATGCAGTTTCACATTTTGCTT2410.11528893991580558No Hit
ATGGTAGGGAGTTGGCTTCAGTTTGGAGATCTTCCATGCTCCTAAAATCAGC2380.11385380788365862No Hit
TAGCCTGGGGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAA2360.11289705319556065No Hit
GGATTAGGGGCGCGCTCCCGGGCCGAGCGCCGCGCGCCATGCTGGCCAAGTT2360.11289705319556065No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG2360.11289705319556065No Hit
TGACCAGGGTCTCTTAGTAAATAAATTAGTAACCTGCTTTCTAAAAATAACC2320.11098354381936472No Hit
AGCATTGGGCGCTGGTTCATGTGTGACAGCTGTTGAAGGCTTTAAAAATAAT2320.11098354381936472No Hit
TCTGCTGGGATTACAGGCGTAGCCACTATGCCCGGCTTGGCTCTCACTTTCG2320.11098354381936472No Hit
TAATGCGGGATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGCTA2320.11098354381936472No Hit
TAGCATGGGCTTTGCAGGCATACAGCAACAGTGGAGCTCCTTTTGAAGGAAA2320.11098354381936472No Hit
GAAACAGGGATCAGACCCAAGCACATTCTCCCAACATCAGCCAATGGAGAGA2310.11050516647531573No Hit
CAATGAGGGAATATATAACAATTTAAAAATGGGTAAAAGACTTGGCGTGTTG2290.10954841178721776No Hit
CACTATGGGTGATTTAAAAAAGAAAAGTGCTGAGTGGACGGAGTAATCAGAG2290.10954841178721776No Hit
TAGACTAGGGCAGGTTAAAATCTCTGTGAGGCAAAGGGATAATATTCTCTAG2250.1076349024110218No Hit
AAGGCAGGAGAATTGCTTGAATCCGGGAAGCAGAGGTTGTAGTGAGCCAAGA2230.10667814772292385No Hit
GATCCTGGGAGGTCATTCAAGGAAGTCAAGTTAACTTAAACATGTCACCTAA2230.10667814772292385No Hit
TGCAATGGGATAAAATTTAATTTTCAAAACAAGATAAACTCTTACCTGATTA2220.10619977037887485No Hit
AGAGTTGGGGGCGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCAG2210.10572139303482588No Hit
GCAGTCCGGGCGATCTACCTAGGGGTGCTCCAGCCCAGCCGGGCTTCAGTTC2190.10476463834672789No Hit
CGTTAAGGGAGTTGGTCGAAGACAGAGGTTCAGGTAAGGATGACTGATAGGA2190.10476463834672789No Hit
TATCCTGGGAATTCCACTCCTGGGTATACACCCAAGAGAACTGAAAACATAT2180.10428626100267892No Hit
AGAATGGGGATTACGATCCTTGGAATGGTCCCTTATTCATTTCATGTACTAT2180.10428626100267892No Hit
AAAATGGGGACCAGAGTAAAAAATTACTTTTGATTGCCACACTTCTAAAATA2170.10380788365862993No Hit
TCGATAGGGGATGTTTCAGGGACTGTAATTTTAGTAAAAATACTATTTCTGT2170.10380788365862993No Hit
ACACACAGGGGATCTTTCTTGTTTTTGATGTAGGTGTTTATTACTAAATACT2160.10332950631458093No Hit
TACGCAGGGAAGTACTTCCGATGGAGGAACTGACTCACCTGGTACTGCGTCC2160.10332950631458093No Hit
AAGCCAGGGGCTTATATGAAGAAGAAAGGTTTAAAATCAGTGACCTAAGCTG2150.10285112897053196No Hit
ATGTATGGGGAACAACATGGGTTTGAACTGCATGGGCACACTTACATGCAGA2150.10285112897053196No Hit
TTGCCAGGGGGACATGCCCAACTTCAAAGGAGATGAAAATTACAATCTTACC2150.10285112897053196No Hit
GGTCTGGGATTACAGCTGTGAGCCACCAAGCCCTGTCGATTTCCATTCTTTA2150.10285112897053196No Hit
GAACTAGGGAACGATGTTGCCACCATCCTGTCTCGCCGTATTCCTGTTGAAT2140.10237275162648296No Hit
GGCAAGTTGTCCTGGGTTCTGTGAGTCCTGAGATGGATTTAATTCTTGATGC2140.10237275162648296No Hit
TTTAATGGGCACCAGATTATTTTTTCTTCAACTGTTTTGACTTTTATTGTTG2140.10237275162648296No Hit
GCGAGGGGGTGAGGCCGCCAGCAGCAATTTGTACCCTGCGTCATTTGGGTGG2130.10189437428243397No Hit
GCTATTGGGGGTATGAGAGGAGAGAGGTCAAGATGGAAAAAAGAAAGAAGCA2130.10189437428243397No Hit
GCAACAGGGCCTGACATCACTGTGGAAGTGGTATTTGAGTTGTGGATCTTTC2120.101415996938385No Hit
CTGGCTGGGGGAGGCCGTGGAGCACAAAGCAGATTCTGATTGGGAGCAACCG2120.101415996938385No Hit
GGCATGGGGAAAAAGTGAGGGTTTATAATTGTCCCTGGGAAAATGTTAAACT2120.101415996938385No Hit
CACATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2120.101415996938385No Hit
TAATGAGGGATAGCAGAAGGACATAGGAGTCAGCTTGAAGAGGCTCCTGCTA2110.100937619594336No Hit
CTTACAGGGACATTGTGGACCAGCAGAAATTTAATAGGGAAATCCTAGAAAT2110.100937619594336No Hit
TACGCTGGGATTTGCCTTTCTACTAAAAATGCTTTTATTCTGTCATTTCAAA2100.10045924225028703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)