FastQCFastQC Report
Sat 31 Dec 2022
SRR5844121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232968
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC10070.43224820576216477No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT8920.38288520311802476TruSeq Adapter, Index 2 (95% over 22bp)
AGCCAGGACCCAATAGCTTCACTGCTGAATTTTACCAAATTCAGTTGTTGTT2740.11761271934342915No Hit
GTGGTGGGGATACACATTTATTCAGACTGTATTTTCCTAAGTATTGATATAT2680.1150372583359088No Hit
TGCCAGGGGTCTCTCTCAAATTGTTAGCCTGGGGAACAAACCACTGAGAGAT2630.11289104082964185No Hit
TTGCTAGGGGAGACAGGGAAAAAGAACGGGAACGAGAAAGAGAAGAGAGAGA2600.11160331032588167No Hit
ATGCTTGGGGAAGGAATAGCCTGAACTCAAACAGATGGTAAGAATAGTACAA2550.10945709281961472No Hit
TAGCTGAGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG2490.10688163181209437No Hit
AATCCAGGGATATGTCAGGAACATTATTGTGAAGAAAATTGAGCTCTCTGAA2430.10430617080457402No Hit
AACTTGGGTACATTTTTTAGGAAAAAGTAGCAGTAAAGTGCCTGCTATGATC2430.10430617080457402No Hit
AGCTTTGGGGCGCCTGCAGAGAGTCTGAAAGGACGTTCACTGCCTGCAGGCA2420.10387692730332063No Hit
AAGTTTGGGCAGAATATCATATAATGTTTATTTTTTGAGGTGTTTTTGTTTT2380.10215995329830707No Hit
TTTCGCGGACCCAATCATCCATGCAGGAATTCAGGCCTGGCATGGCCAGAGT2380.10215995329830707No Hit
AGCACTGGGATGAGGAGTGACAGAATATCAAAATAATTTGTGGCTGTGGATT2350.10087222279454688No Hit
GAATAAGGGAATTGCTTCAAAGAGAATAAAATACCTAGGAATCCAGCTTACA2350.10087222279454688No Hit
TTTGCTGGGGACCGCGAAGGGAGGAGTGGCAACATGGCGTCTTCGGGAGCTG2330.1000137357920401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)