FastQCFastQC Report
Sat 31 Dec 2022
SRR5844120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232806
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC10030.43083082051149885No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT9550.4102127952028728TruSeq Adapter, Index 2 (95% over 22bp)
GTGGTGGGGATACACATTTATTCAGACTGTATTTTCCTAAGTATTGATATAT2550.10953325945207597No Hit
TGCCAGGGGTCTCTCTCAAATTGTTAGCCTGGGGAACAAACCACTGAGAGAT2540.10910371725814626No Hit
AGCACTGGGATGAGGAGTGACAGAATATCAAAATAATTTGTGGCTGTGGATT2480.10652646409456801No Hit
ACGTTTGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT2470.10609692190063831No Hit
TTGCTAGGGGAGACAGGGAAAAAGAACGGGAACGAGAAAGAGAAGAGAGAGA2450.10523783751277888No Hit
CAATCAGGGCAAGCATTTATCCTTTTTAAAGCGAACTGTTGACAATTAGATT2450.10523783751277888No Hit
TAGCTGAGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG2430.10437875312491945No Hit
TCTAATGGGTCGAGTGTTTTCTCAGTATTGGAGAAATGCTTAGGTCCTATGA2420.10394921093098974No Hit
AGCCAGGACCCAATAGCTTCACTGCTGAATTTTACCAAATTCAGTTGTTGTT2410.10351966873706005No Hit
TAAATCGGGAACCATTGTGATGGCTGAGGACAAAGATTGGCTGACATTCATT2390.10266058434920061No Hit
CTTGGTGGGAATCAGAATTCGAAGGATTTTCCTTTGTTAACTCTGAATTTTT2390.10266058434920061No Hit
CCATTCCCACTAAAAATTCACAGCATTCTAGGAATAGAAAAGAATGTCCCTA2380.10223104215527092No Hit
AACTTGGGTACATTTTTTAGGAAAAAGTAGCAGTAAAGTGCCTGCTATGATC2380.10223104215527092No Hit
TAATCGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA2370.1018014999613412No Hit
TGCTACGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2360.10137195776741148No Hit
GTTGCCGGGGTTTGCATTGCAATAAGATCTACTGGCAGAATAATTTTTGCTT2360.10137195776741148No Hit
ACAAAAGGGCAGCTGAAGGTTCATGGGTTTTAAGTGCTTGTGGCTCACTGAA2350.1009424155734818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)