FastQCFastQC Report
Sat 31 Dec 2022
SRR5844119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230202
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9640.4187626519317817No Hit
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT9510.4131154377459796TruSeq Adapter, Index 2 (95% over 22bp)
GTGGTGGGGATACACATTTATTCAGACTGTATTTTCCTAAGTATTGATATAT2590.11250988262482516No Hit
TGCCAGGGGTCTCTCTCAAATTGTTAGCCTGGGGAACAAACCACTGAGAGAT2580.11207548153360962No Hit
AATTTTGGGAATGTAATGACTGTCAAACTGGATACTTGGCGAACAATCCTTT2560.11120667935117852No Hit
ACGTTTGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT2530.10990347607753191No Hit
TGCTACGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT2430.1055594651653765No Hit
CTTGGTGGGAATCAGAATTCGAAGGATTTTCCTTTGTTAACTCTGAATTTTT2380.10338745970929879No Hit
AGCTTTGGGGCGCCTGCAGAGAGTCTGAAAGGACGTTCACTGCCTGCAGGCA2380.10338745970929879No Hit
CTAGGTTTGGGAAGAGACTGAATAAAAGGTAGATATTCTCTCCATGTGAAAC2360.1025186575268677No Hit
TAGCTGAGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG2360.1025186575268677No Hit
TTGCTAGGGGAGACAGGGAAAAAGAACGGGAACGAGAAAGAGAAGAGAGAGA2330.1012154542532211No Hit
ACTTATGGGGAAGAGAATGGGAGAAGTGGAGGAAGGGCTCTCAGGAAGACAG2320.10078105316200556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)