FastQCFastQC Report
Sat 31 Dec 2022
SRR5844112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213107
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT13510.6339538353972418TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9910.4650246120493461No Hit
GGCTAAGGGGAGGAATAAATATTCGATGTTACTAAGAGACGTGAAGAAATGA2400.11261948223193043No Hit
TGTGCCGGGTTTCACTGAAAATGAAGAATGATAATGAGAAGAGAGCCTAGTT2280.10698850812033392No Hit
TATAAAGGGAGGTGCTGAGTGTGGCCACTGACGGGAAGGTGCTACTCTGGCA2190.10276527753663653No Hit
ATTGTAGGGATCCAAACAAGATTTCTGAGGAGGAAAAAAAATAAAAATTGTA2170.10182678185137042No Hit
AATCTTGGGACTACATTTAAGCTCTGATTCTATATAAAATGTCACCAAAAAG2160.10135753400873738No Hit
GTGCTAGGGAACTGGGTTTGAATCAAGGTCCCGTCCCTTGCTGATTGTATCA2150.10088828616610435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)