FastQCFastQC Report
Sat 31 Dec 2022
SRR5844111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214194
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT13070.6101944965778687TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9350.43652016396350973No Hit
CTCAAATCGGGTGGGGCACTGAGTGACTCCAAGTCTGTGAGCAACCTTTCTG2660.1241864851489771No Hit
TGTGCCGGGTTTCACTGAAAATGAAGAATGATAATGAGAAGAGAGCCTAGTT2460.11484915543852768No Hit
TATAAAGGGAGGTGCTGAGTGTGGCCACTGACGGGAAGGTGCTACTCTGGCA2380.11111422355434793No Hit
ATTGTAGGGATCCAAACAAGATTTCTGAGGAGGAAAAAAAATAAAAATTGTA2310.10784615815569064No Hit
TACGCAGGGGGGGCGGATCGTGGTGGCTGCGCTTGCGAGGACCCTGAGGTAG2310.10784615815569064No Hit
GTGCTAGGGAACTGGGTTTGAATCAAGGTCCCGTCCCTTGCTGATTGTATCA2300.10737929167016816No Hit
ATCGTGGGGGGCGATCCCGCAGGGCAGCGACGCGACTCTGGTGCGGGCCGTC2230.10411122627151087No Hit
TCGCTACGGGCACTTACAAAATTGCTACTCTTCATCGATGCCGTATTTACAT2220.10364435978598839No Hit
TGCTTTGGGAGACAAACAGATAAACAAACGGAATCTGGGAGAGGAAGCATTA2200.10271062681494346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)