FastQCFastQC Report
Sat 31 Dec 2022
SRR5844110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210539
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGCCGTCT12290.5837398296752621TruSeq Adapter, Index 2 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACGGTGTATCTCGTATGC9220.4379236151021901No Hit
AGCCTGGGTGATATAGAGTGATACCCTGTTTCTTTAAAAAATAAAAATAAAG2450.11636798882867308No Hit
GTGCTAGGGAACTGGGTTTGAATCAAGGTCCCGTCCCTTGCTGATTGTATCA2330.11066833223298297No Hit
TGCTTTGGGAGACAAACAGATAAACAAACGGAATCTGGGAGAGGAAGCATTA2200.10449370425431867No Hit
TTCCTGGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG2200.10449370425431867No Hit
TTCGTAGGGATTCAAATTTCTTGTAATTTAGTGAGGTGAACGACTTCAGATT2110.1002189618075511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)