FastQCFastQC Report
Sat 31 Dec 2022
SRR5844082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10977
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3192.9060763414411954No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC2732.487018311013938No Hit
TGGCTAGGGAGTGCAGCGAGCTGAGGCACTTGTCCGTTCGTCTTACCATCTA1070.9747654186025325No Hit
CAAAAGGGGGACCTTCCGCCATTTTCCTGCCTGTGAGGGTGGACAGATCGCG1000.9109957183201239No Hit
TGACCTGGGGGTTGGAGGACTTGGTTTGTGATGTGGTAGATCGAATATTGAA820.7470164890225016No Hit
GTCAACGGGCTAGCCACGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAG760.6923567459232942No Hit
AGCGTAGGGGGAGGACCCGGGTCTGGCGCTGTGGGCCGGGAGCCGTGGGGCG680.6194770884576842No Hit
AGAATTGGGTGCGGAAGAGTCAAGGGAATGTCATCAACATCTCCAGCCTGGT660.6012571740912818No Hit
GCACCCAGCATGCAGCTCTGCCTCCGCAGCCTCAGTGCGCTTTCCTGCGCGC650.5921472169080806No Hit
GCTCACGGGGAGCAATAAGTCCATGGAGATCGAGGTGTTGGTGGACGCCGAC650.5921472169080806No Hit
ATTGCAGGGTGTACTTGTTTTCTTGGTAGCAGTTTTGAAATATTCTGTACAG610.5557073881752755No Hit
ATAGCTAGGGGAGCTGGAGATGAACTCGGACCTCAAGGCTCAGCTCAGGGAG590.5374874738088731No Hit
ATGTATGGGGATTACTGAACTGTGTTGTGACTTCTTTTGTTCTAGGTGCCTT580.5283775166256719No Hit
ATTTGTGGGAGAGAAAGATGTTGTTAATGTCCTACAGGCAGTCGGTGAATCC570.5192675594424706No Hit
CAAATTGGGGAGATGGTGCTGCAGATGCTGGTTGGTTCGGTATGTGGGGACC540.4919376878928669No Hit
AACATGGGGGGTTTCTAGGGTTGTTACGAAGCTGCAGGAGCGAGATGGAGGT510.46460781634326315No Hit
CTCTTAGGGCAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGG490.44638790197686073No Hit
CATATGGGAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC450.4099480732440558No Hit
TCTAACAATGGGGTGTTACAATTTATTTTCTGGTAATATCTTGTGTTTTCAG430.3917281588776533No Hit
GGCTGTGGGCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTT410.3735082445112508No Hit
TAATGTGGGTGTGGGTGATGACCTGACAGTGACCAACCCAAAACGTATTGAG410.3735082445112508No Hit
GGCTGTTTGGTTAGCATCTCATCTTGCATTCTGATCAATTGGCAAGAAAGGG410.3735082445112508No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT410.3735082445112508No Hit
CCCACCTCAGTGTGGGTTCCATCTTACTTGTCACGTTTGCATCACTGGTTGC390.3552883301448483No Hit
TAGATAGGGAGAGAGAATGCCATTAAATGGAAGAATGTACTGATTGTAGTGA390.3552883301448483No Hit
TTTGGTGGGATCTTATGTAGAAATATTTTGATAATATTTTTACAGTGAGCTT340.3097385442288421No Hit
AGGCCAGGGCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCAC340.3097385442288421No Hit
AAGCCGGGACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGG330.3006285870456409No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA320.29151862986243965No Hit
CAGTTGGGGAGAGGGACTGAAAGCAGAGACATAACGGCACAAAACGAGATGG320.29151862986243965No Hit
ATTTTAGGGGGATAAAGGGTGCCCAAGCCTCTCCTGATGCATTTGTAAACAA310.2824086726792384No Hit
GAAGCTGGGAAGACATTACAATATAATTTTTTTAAAAACAGGAGACAATACT300.2732987154960372No Hit
AGCCGGGGGCATGGAGTGAGTGTGGGTGGCCAGCTCCCCCCGCGCGCCCACC250.22774892958003098No Hit
AACACCGGGTGAATGAAGAATGCATACCAGTGTTTTAAAAGGTATTTTTATG240.21863897239682972No Hit
GTTAATGGGTAGGCCTCTCTTTTTATTTTGCTTTGGTTTTGTTTGTTTTTCT230.2095290152136285No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCTTATAC220.20041905803042723No Hit
ACATCTGGGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCCC210.19130910084722602No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT200.1821991436640248No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTACTGTCTCTTA180.16397922929762232No Hit
CAAGCTGGGGCTACTCAGGAGGCAAAGATGGGAGGATTACTTGAGCCCAGGA170.15486927211442106No Hit
TCAACAGGGCAAGACATTTGCTAAGACTCTCATGCTAACTTGGGAGGCAGGT170.15486927211442106No Hit
GCATATGGGACTAATCATTTAAAATGTATACTAATCTTCAATTTAAATAAAA160.14575931493121982No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT160.14575931493121982No Hit
AACTGGGGAAATTCATCATATGTTATTAAAGGGAAACTAGGATGTTTTCTTG150.1366493577480186No Hit
TCGTGCGGGCGATCTTTCCTAGGGATGCCGGTGACCTGAGGACTCCACTCAC140.12753940056481736No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTAACTCGTATGC140.12753940056481736No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTACTGTCTCTTATACAC140.12753940056481736No Hit
ACATCTGGGGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCC130.1184294433816161No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTC130.1184294433816161No Hit
AAAACTGGGGGTGGGGGCAGACTTTGCACTTACTGCAGTGCAACACTTGCAC130.1184294433816161No Hit
TGTTGCGGGGGTGAAAAAAGACAAGCCAGTAAAAACAGAGACCAAACCTTCA130.1184294433816161No Hit
ATGTACGGGAAGTGGTCACCTGGGCATTGAGATTTGTAAAACACCTTCTGGT130.1184294433816161No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTAT120.10931948619841486No Hit
CAAGCAGGGACTGAGGATTGGGTAAATACAAAACCACTGTAGAAAAACTGAA120.10931948619841486No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTAT120.10931948619841486No Hit
ATCCTTGGGGGCTCACTGAAACCTCCGCCTCCCTGGTTCAAGCGATTCTCCT120.10931948619841486No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCT120.10931948619841486No Hit
CACAGTGAAGCTAAAAAAGTTTGAGTTTCGGGGCCCCTTTCTTGCCCGGGCC120.10931948619841486No Hit
ATTAGAGGGGGCCAACCTGGGGACTCTGGCCGGACGGCCAATGGGCTGCCTG120.10931948619841486No Hit
TTCCTAGGGAAATCTTGCTGCTATAAAGACACATGCACATGTATGTTTATTG110.10020952901521361No Hit
GTCGATGGGATGAATATATTTGACATCTACCTCAGCAAACAAATTAATGCTA110.10020952901521361No Hit
TGTACAGGGACTGGCGCGGGTTGAGTTCCCTGTTGCCCTTGGTCTCGGGGTC110.10020952901521361No Hit
TGACCTGGGATTCTACTTGATTTTTTCTGTAATTAACAGATCTTTTCATCTT110.10020952901521361No Hit
ACGCTTTGGGGAAGTTGACTTACTGAAGAATGGAGAGAGAATTGAAAAAGTG110.10020952901521361No Hit
CACGCTGGGGTTTCTTTATCAATTTAGAAAGACTTTAAAAAGCCATTTACTC110.10020952901521361No Hit
AGTCTGGGGGATCTGAACCGGGAAGTTCGAAGCATTCAGCAAACAATGGCAA110.10020952901521361No Hit
TTATTAGGGCGGTTTTTTTGTAGCTATGTGGCTCTTGTGAATGGGTAGAATG110.10020952901521361No Hit
TCGTTGGGGAAGTATTAGTTGTTTCATACAAAAGTCAGATTTCCTTTGGGGT110.10020952901521361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)