FastQCFastQC Report
Sat 31 Dec 2022
SRR5844081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11203
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3493.11523699009194No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC2902.588592341337142No Hit
CAAAAGGGGGACCTTCCGCCATTTTCCTGCCTGTGAGGGTGGACAGATCGCG1281.1425511023832902No Hit
TGGCTAGGGAGTGCAGCGAGCTGAGGCACTTGTCCGTTCGTCTTACCATCTA1020.9104704097116844No Hit
GCACCCAGCATGCAGCTCTGCCTCCGCAGCCTCAGTGCGCTTTCCTGCGCGC810.7230206194769259No Hit
AGCGTAGGGGGAGGACCCGGGTCTGGCGCTGTGGGCCGGGAGCCGTGGGGCG790.7051682585021869No Hit
TGACCTGGGGGTTGGAGGACTTGGTTTGTGATGTGGTAGATCGAATATTGAA760.6783897170400786No Hit
GTCAACGGGCTAGCCACGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAG760.6783897170400786No Hit
ATAGCTAGGGGAGCTGGAGATGAACTCGGACCTCAAGGCTCAGCTCAGGGAG730.6516111755779702No Hit
AACATGGGGGGTTTCTAGGGTTGTTACGAAGCTGCAGGAGCGAGATGGAGGT650.5802017316790146No Hit
AGAATTGGGTGCGGAAGAGTCAAGGGAATGTCATCAACATCTCCAGCCTGGT640.5712755511916451No Hit
CAAATTGGGGAGATGGTGCTGCAGATGCTGGTTGGTTCGGTATGTGGGGACC630.5623493707042756No Hit
ATTTGTGGGAGAGAAAGATGTTGTTAATGTCCTACAGGCAGTCGGTGAATCC620.5534231902169062No Hit
CTCTTAGGGCAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGG600.5355708292421673No Hit
GCTCACGGGGAGCAATAAGTCCATGGAGATCGAGGTGTTGGTGGACGCCGAC560.49986610729268943No Hit
ATTGCAGGGTGTACTTGTTTTCTTGGTAGCAGTTTTGAAATATTCTGTACAG540.4820137463179506No Hit
CATATGGGAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC510.4552352048558422No Hit
CCCACCTCAGTGTGGGTTCCATCTTACTTGTCACGTTTGCATCACTGGTTGC470.41953048290636435No Hit
AGGCCAGGGCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCAC470.41953048290636435No Hit
TAGATAGGGAGAGAGAATGCCATTAAATGGAAGAATGTACTGATTGTAGTGA440.392751941444256No Hit
GGCTGTTTGGTTAGCATCTCATCTTGCATTCTGATCAATTGGCAAGAAAGGG430.38382576095688653No Hit
TCTAACAATGGGGTGTTACAATTTATTTTCTGGTAATATCTTGTGTTTTCAG430.38382576095688653No Hit
ATGTATGGGGATTACTGAACTGTGTTGTGACTTCTTTTGTTCTAGGTGCCTT420.3748995804695171No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT410.3659733999821477No Hit
AAGCCGGGACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGG400.3570472194947782No Hit
GGCTGTGGGCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTT400.3570472194947782No Hit
GAAGCTGGGAAGACATTACAATATAATTTTTTTAAAAACAGGAGACAATACT400.3570472194947782No Hit
AGCCGGGGGCATGGAGTGAGTGTGGGTGGCCAGCTCCCCCCGCGCGCCCACC390.34812103900740876No Hit
TAATGTGGGTGTGGGTGATGACCTGACAGTGACCAACCCAAAACGTATTGAG360.32134249754530037No Hit
CAGTTGGGGAGAGGGACTGAAAGCAGAGACATAACGGCACAAAACGAGATGG350.3124163170579309No Hit
AACACCGGGTGAATGAAGAATGCATACCAGTGTTTTAAAAGGTATTTTTATG310.2767115951084531No Hit
TTTGGTGGGATCTTATGTAGAAATATTTTGATAATATTTTTACAGTGAGCTT300.26778541462108363No Hit
CAAGCTGGGGCTACTCAGGAGGCAAAGATGGGAGGATTACTTGAGCCCAGGA290.2588592341337142No Hit
TCGTGCGGGCGATCTTTCCTAGGGATGCCGGTGACCTGAGGACTCCACTCAC250.22315451218423635No Hit
ATTTTAGGGGGATAAAGGGTGCCCAAGCCTCTCCTGATGCATTTGTAAACAA240.21422833169686692No Hit
GTTAATGGGTAGGCCTCTCTTTTTATTTTGCTTTGGTTTTGTTTGTTTTTCT210.18744979023475855No Hit
GCATATGGGACTAATCATTTAAAATGTATACTAATCTTCAATTTAAATAAAA210.18744979023475855No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTACTGTCTCTTA210.18744979023475855No Hit
TGCAGTGGAGATTTAGATCTCTCAGAAATATAAAAGACAACAAAAGGAAGCA200.1785236097473891No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT200.1785236097473891No Hit
ACATCTGGGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCCC180.16067124877265018No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA180.16067124877265018No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCTTATAC180.16067124877265018No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTAT160.14281888779791127No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTACTGTCTCTTATACAC160.14281888779791127No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT150.13389270731054181No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCT150.13389270731054181No Hit
GAAATTGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA140.12496652682317236No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCC140.12496652682317236No Hit
AGTCTGGGGGATCTGAACCGGGAAGTTCGAAGCATTCAGCAAACAATGGCAA140.12496652682317236No Hit
AGTGCCGGGGAACGACTGGTTGTATGCCTTCAACCCACTTCTAGCTGGCACA140.12496652682317236No Hit
AGCAGAGGGAGCCAAGCTAGTGTCAGTGTCGTCACCTAATCCTGGAGCACAC140.12496652682317236No Hit
GCCTTGGGGGACGAGGGCGCCCGCCCAGTGACGGCCACTCCTGCGGTGAACA130.11604034633580292No Hit
TCAACAGGGCAAGACATTTGCTAAGACTCTCATGCTAACTTGGGAGGCAGGT130.11604034633580292No Hit
GCTATAGGGAATTTTTCCTGCAACGTTGCCTTTTTATATTCATAGTTTCTAT120.10711416584843346No Hit
ACATCTGGGGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCC120.10711416584843346No Hit
TGTACAGGGACTGGCGCGGGTTGAGTTCCCTGTTGCCCTTGGTCTCGGGGTC120.10711416584843346No Hit
ACATTTGGGGATGACTTAGGTTGGACATTCAATGTTGTGCTATGAATTTCCT120.10711416584843346No Hit
AGCCAGGGGTTACAAGAGAGGAGACACATTCTTCGTCCTTACAGCGTGATGG120.10711416584843346No Hit
CCTAGGGGTAGCAATAGTAAAGAACGGATCAAAGCCACCGTTTTTCTGTCAA120.10711416584843346No Hit
GCATTAGGGGGCAGAAGATTTGTTGCCGTCATGTCGGCTGCGATTGCAGCTC120.10711416584843346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)