FastQCFastQC Report
Sat 31 Dec 2022
SRR5844080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10963
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3172.8915442853233606No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC3102.827693149685305No Hit
TGGCTAGGGAGTGCAGCGAGCTGAGGCACTTGTCCGTTCGTCTTACCATCTA1131.030739761014321No Hit
TGACCTGGGGGTTGGAGGACTTGGTTTGTGATGTGGTAGATCGAATATTGAA790.7206056736294809No Hit
CAAAAGGGGGACCTTCCGCCATTTTCCTGCCTGTGAGGGTGGACAGATCGCG750.6841193104077351No Hit
GCACCCAGCATGCAGCTCTGCCTCCGCAGCCTCAGTGCGCTTTCCTGCGCGC750.6841193104077351No Hit
GTCAACGGGCTAGCCACGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAG750.6841193104077351No Hit
GCTCACGGGGAGCAATAAGTCCATGGAGATCGAGGTGTTGGTGGACGCCGAC730.6658761287968622No Hit
CTCTTAGGGCAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGG730.6658761287968622No Hit
ATTTGTGGGAGAGAAAGATGTTGTTAATGTCCTACAGGCAGTCGGTGAATCC720.6567545379914257No Hit
AGAATTGGGTGCGGAAGAGTCAAGGGAATGTCATCAACATCTCCAGCCTGGT710.6476329471859893No Hit
ATTGCAGGGTGTACTTGTTTTCTTGGTAGCAGTTTTGAAATATTCTGTACAG690.6293897655751163No Hit
AGCGTAGGGGGAGGACCCGGGTCTGGCGCTGTGGGCCGGGAGCCGTGGGGCG690.6293897655751163No Hit
GGCTGTGGGCAATTACCTGCTGATGAGTGCAACAAGCTGAAAGAAGAGATTT650.5929034023533705No Hit
ATAGCTAGGGGAGCTGGAGATGAACTCGGACCTCAAGGCTCAGCTCAGGGAG640.583781811547934No Hit
AACATGGGGGGTTTCTAGGGTTGTTACGAAGCTGCAGGAGCGAGATGGAGGT560.5108090851044422No Hit
ATGTATGGGGATTACTGAACTGTGTTGTGACTTCTTTTGTTCTAGGTGCCTT560.5108090851044422No Hit
CAAATTGGGGAGATGGTGCTGCAGATGCTGGTTGGTTCGGTATGTGGGGACC540.4925659034935693No Hit
TCTAACAATGGGGTGTTACAATTTATTTTCTGGTAATATCTTGTGTTTTCAG470.428714767855514No Hit
AGGCCAGGGCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCAC450.41047158624464103No Hit
TAGATAGGGAGAGAGAATGCCATTAAATGGAAGAATGTACTGATTGTAGTGA430.39222840463376807No Hit
CCCACCTCAGTGTGGGTTCCATCTTACTTGTCACGTTTGCATCACTGGTTGC390.35574204141202226No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT390.35574204141202226No Hit
TTTGGTGGGATCTTATGTAGAAATATTTTGATAATATTTTTACAGTGAGCTT360.32837726899571285No Hit
GGCTGTTTGGTTAGCATCTCATCTTGCATTCTGATCAATTGGCAAGAAAGGG360.32837726899571285No Hit
AAGCCGGGACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGG350.3192556781902764No Hit
CATATGGGAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC350.3192556781902764No Hit
GAAGCTGGGAAGACATTACAATATAATTTTTTTAAAAACAGGAGACAATACT340.31013408738483994No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA320.291890905773967No Hit
AACACCGGGTGAATGAAGAATGCATACCAGTGTTTTAAAAGGTATTTTTATG300.273647724163094No Hit
CAGTTGGGGAGAGGGACTGAAAGCAGAGACATAACGGCACAAAACGAGATGG290.2645261333576576No Hit
AGCCGGGGGCATGGAGTGAGTGTGGGTGGCCAGCTCCCCCCGCGCGCCCACC290.2645261333576576No Hit
CAAGCTGGGGCTACTCAGGAGGCAAAGATGGGAGGATTACTTGAGCCCAGGA290.2645261333576576No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT260.23716136094134818No Hit
TCGTGCGGGCGATCTTTCCTAGGGATGCCGGTGACCTGAGGACTCCACTCAC230.20979658852503877No Hit
ATTTTAGGGGGATAAAGGGTGCCCAAGCCTCTCCTGATGCATTTGTAAACAA230.20979658852503877No Hit
ACATCTGGGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCCC220.2006749977196023No Hit
GTTAATGGGTAGGCCTCTCTTTTTATTTTGCTTTGGTTTTGTTTGTTTTTCT210.19155340691416584No Hit
AGCAGAGGGAGCCAAGCTAGTGTCAGTGTCGTCACCTAATCCTGGAGCACAC210.19155340691416584No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCTTATAC190.17331022530329288No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTAACTCGTATGC190.17331022530329288No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTACTGTCTCTTATACAC180.16418863449785642No Hit
GTTACTGGGAGCTAACAACCCAATAAGGGTGACTGAGAAAAGGCATGAGAAC170.15506704369241997No Hit
GCTACAGGGATCAAGGACATTTTAAAGCTTTGTTTAAAAACTGTAATTCATA160.1459454528869835No Hit
GATAATGGGCGATCTAACACGGGGATGTTTAACGGCCGCGGTACCCTAACCG160.1459454528869835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCCTGTCTCT160.1459454528869835No Hit
GCATATGGGACTAATCATTTAAAATGTATACTAATCTTCAATTTAAATAAAA160.1459454528869835No Hit
TAATGTGGGTGTGGGTGATGACCTGACAGTGACCAACCCAAAACGTATTGAG150.136823862081547No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGT150.136823862081547No Hit
GTTATAGGGAAGACCAGGAAGCAGAAGCACAGCAAAATAAGAAGTCTTCAGG150.136823862081547No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTAT140.12770227127611056No Hit
GTTGCGGGCGATCTCAAATAGGGCTGTTTTGTCCTAGTACATGCTTGGGGCC140.12770227127611056No Hit
GTCCATGGGATAGAGGACAAATAAACATTTATTCTTTATAGTTATTTTATGG140.12770227127611056No Hit
GGCATGAGGGCGTCGCCATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGT140.12770227127611056No Hit
GCCTTGGGGGACGAGGGCGCCCGCCCAGTGACGGCCACTCCTGCGGTGAACA130.11858068047067409No Hit
ACCGAGGGGGTAAATGTTGAAAATATATCAAAATCCAGTATTTGTAAAGCAT120.10945908966523761No Hit
GCTCGTGGGACCCACTGCTTCCTGACTCGCTCTACTCCGTGGAAGCACGCTC120.10945908966523761No Hit
AGTCTGGGGGATCTGAACCGGGAAGTTCGAAGCATTCAGCAAACAATGGCAA120.10945908966523761No Hit
ATAGTGGGGAAGCCTTCATTCCTTCAAAAGGTCCTGACAAAATACTTAGGTT120.10945908966523761No Hit
GGTCTGGGCAATGTGATACTCTCCCTCTATTGCTAGATCTTCGCATCAGAAG120.10945908966523761No Hit
ATTAAAGGGAACAGTGTTGCAGTAAGCTGTTGATAGTGATTCTTTTTAATTA120.10945908966523761No Hit
ATGATTGGGTTGTGTATATCTATATTTGATTATTTAATCTTATGTGAAGATA120.10945908966523761No Hit
AACCAGGGAATGAAATTTTGATGTGTTGAACTGCTTTCCTGGTGTCAGAGAA110.10033749885980114No Hit
ACAACTGGGTAGACATCCACAGAACTCTCCACCCCAAATCCACAGAATATAC110.10033749885980114No Hit
ATACAAGGGCAAGGCATGAAACATGATACCTCACTTTGCTGTCATATACACT110.10033749885980114No Hit
AAAATGGGGCAAAAAGACTTTCCTATACAGATACTTTCTATTGACACTCTTG110.10033749885980114No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCC110.10033749885980114No Hit
TACCTAGGGCGCGGGGGGAACTCATTTGTCCTTCTAGATTTTAGCTTAAAGG110.10033749885980114No Hit
ACCTGGGATTGTAATCAAATAAGTTGATTCTCTTTCAAAGGTGTATAAATGT110.10033749885980114No Hit
GATTCTGGGGGGCATATGAAAAAGATCTCATCATCACTGGTCATTGGAGAAA110.10033749885980114No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTC110.10033749885980114No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTAT110.10033749885980114No Hit
TGCAACGGGCAGGACCAATCGTGAAGTTCACTTTCTCTCTTTGTATTCATAG110.10033749885980114No Hit
TCAACAGGGCAAGACATTTGCTAAGACTCTCATGCTAACTTGGGAGGCAGGT110.10033749885980114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)