FastQCFastQC Report
Sat 31 Dec 2022
SRR5844075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132022
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC6370.4824953416854767No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT5850.44310796685400916No Hit
AGCTACAGGGGAAGATGGGAAATGTAGTTTCTGGCTAAATATCAGTGTATAA1660.12573661965430005No Hit
GCTTCTGGGATACATGGAAAATGTTTTTCTGATCCATTATACTCTTTCTTTA1630.12346427110633076No Hit
CGTAAAGGGGTATAAATAGATTTAAAAAATAAAAGTTTTAAAAAATGTACAT1600.12119192255836148No Hit
ACTTCCGGGGTATTATGATGGGATAAAAATGGAAGTTTCCTTCTGAAGTCTT1600.12119192255836148No Hit
CACATGGGGGATTACTGCTCCTCTGCTCTAAAATTGGTGTTTGGGTGATCAG1550.1174046749784127No Hit
CATAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1540.11664722546242293No Hit
AGATCGGGGAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCC1510.11437487691445365No Hit
TTTAATGGGAGGATAAGAACCTCACTTCCTTACTCCTCCAAAAAGAAGTGGG1490.11285997788247414No Hit
ACAACTGGGATTTTATTTCTGTCCTTAAAACACACATACAAAATTACAATAT1460.11058762933450486No Hit
AAGAATGGGGAACACGGATAGATTCAAGGCATTTAGTAGTAATCTAGTTCTT1460.11058762933450486No Hit
TAAGCTGGGTCAGCTCATGGTTGCACTGCTATCAAGAAATACCCAAGACTGG1460.11058762933450486No Hit
AACTATGGGTCTCAGCAAGGCTTTTCCTGTTGGGAGTCACAGTAAACAGAAA1450.10983017981851509No Hit
CTACCTGGGTGATCACTGTTAGAGCATAGCCAAGCGTACTAACGCAATATGG1430.10831528078653557No Hit
CTAAAGGGGCCATGGAAGAATGGAGATAGATTCTTTACAGATCAAGAGATTG1420.10755783127054581No Hit
GAACAAGGGGGAAAACAAGACTTTTCATTTTCAGTTTAATTCCTCAGGGAAC1420.10755783127054581No Hit
TTAGGGGGGGTGCTCGTTTTGTTGAATTGGTACATCAACAGAAAACTCCAAA1410.10680038175455607No Hit
GCTGATGGGATGAACCAGAAATCCTTACATTTATTTTCGGTTGATTTTGACA1400.1060429322385663No Hit
GTTGCCGGGGTATCCTTTTGACAACTTGTTTTCAACAATGGCAAATAAATAA1400.1060429322385663No Hit
GATTGAGGGAGAATGGTAAATTAATATTGGCTTCAACTTAAAGTCACCAGCT1390.10528548272257653No Hit
ATGAAAGGGAGGCTGAGGCAGAGAGGATTTGTGAAATTAGAGAGACGGTGTG1390.10528548272257653No Hit
GCCTCTGGGCACTGCTTGGGTGATGGATGCACCAAAATCTCAGAAATGATCA1380.10452803320658678No Hit
AAACTGGGGGGAGCCGTGGCGCGCGGGTGGCCGAAAGGCGAAAGTGAGATTT1380.10452803320658678No Hit
GCCATAGGGCTTGATTCAAACCTCACAAGGCAATATTCTGAATTGTTAACTA1370.10377058369059702No Hit
TCTGTAGGGGCCCCTCCATCGTCCACCGCAAATGCTTCTAGGCGGACTATGA1360.10301313417460728No Hit
GTGAAGGGCAAGTAGCTGGGACTTCAGACGCCCGCCATCATGCCCGTTTAAT1360.10301313417460728No Hit
CTAAATGGGAGGAGAATACAAGAAGCTGAATTGAATTTACACAAAACTGACA1340.10149823514262774No Hit
CGTCATGGGATCATTTTTGAGAGTGAAAGACCTGAAATACTAAGGAACTGAG1340.10149823514262774No Hit
TCAAGGCGGGCTCTTCCGATCTACAACTGGGGATGCACTGTTGCCAAGGAAG1330.10074078562663799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)