FastQCFastQC Report
Sat 31 Dec 2022
SRR5844067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30242
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC4311.4251702929700416No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3951.3061305469214999No Hit
TCTAAAGGGCAGCTACTATGGGTGATCTCAACATGATTTTTATTTGAAAGTG1640.5422921764433569No Hit
ATTTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1490.4926922822564645No Hit
TGGTGGGACCTAGAAATCCCTCTGGACACTTGTGGGTTCTTTAGGGTTTGAG1450.4794656438066265No Hit
CGATATGGGCACCTCAGGCCGCTGCTCAGATGCCTCTGCTGGAGCTGGCACT1420.4695456649692481No Hit
AGACACGGGGATTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAG1410.4662390053567886No Hit
AGTTTTGGGAAGCCAACAACAGGGTCTTGAGATAACCTTTGAAGCTTATTGT1390.4596256861318696No Hit
CGGCGGCGCGTCTGGAGGAGGCTGCAGCAGCGGAAGACCCCAGTCCAGATCC1380.4563190265194101No Hit
AGCTGGGACTCAGAGGGTGTCTCTGCAGTCTGGTGGATCTTGTCCATTGGGC1370.4530123669069506No Hit
TTGCTTGGGAAGAAACTGAAAGCTGTATGTCAACTTTATAAACATAATTCAA1310.4331724092321936No Hit
TTGAGCCGGGGCAGAGCTCGAGGAAGAACAGTCCCAGGGCTCCACCACAAAT1280.4232524303948152No Hit
AATTGGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGT1280.4232524303948152No Hit
TTGATGGGGCAGCTGATGGACACTTGCGTTGCTTCCACCTTTGGGTTCTTGT1260.4166391111698962No Hit
CGCTATGGGGGGCGCACGCAGCCGCACTGGGGGTGCAAGACTACCTCCTGTT1250.4133324515574367No Hit
TCGCCAGGGAGCCTTTAATTAGAATCCTAGTTTTCTCTCTAATCCAGTGGCA1250.4133324515574367No Hit
TGAACCCGGGAGGCAGAGGTTGCAGTGGGCTGAGATCGCACCACTGCACTCC1180.39018583427022024No Hit
GATCACGGGTGTGGAAAAAAACAAGCTCACTTTGGGATTACCCACCCCGAAA1150.38026585543284175No Hit
CTTAAAGGGAACTCCCTGAATGACGACATCAGCCTCTACAAGAGCTTCCTCC1140.37695919582038223No Hit
GTTACAGGGTCCTATAATGGGAGCATCACCACTTATTAAAACACACATAGAA1130.3736525362079227No Hit
GACGCGGAGCCTTGAAGACTGTGCTGGTGAGAAGAAGTCTGGGTGGGAGATG1120.37034587659546325No Hit
CGGGATGGGCAGATGCTTTTTCGTGTACATGATACTAGTAGACACTTTTCTC1120.37034587659546325No Hit
TTAGTGGGGGGGTGCGGGTGCCCGGGTGAGGGGCGGAGCTGGGGGCATGGCG1090.36042589775808476No Hit
AGTTTGGGAGCACCTAGTGCGAGACAGTGTGTTCCAGCGCTGCGGCCTCCGC1090.36042589775808476No Hit
TGTCATGGGAAGTCAACCTAGAACTATACACAAATACAAACATGCCTAAAAT1080.3571192381456253No Hit
AACACAGGGGCCCCATAGTTCAGCTTGTGAAACTTTCACGTCGTTGTGTTGG1080.3571192381456253No Hit
TACGAAGGGATTTATTAATATGTAAAAGAGTGATAAACAAGGGAAAATAAAG1030.3405859400833278No Hit
CTCCAGGGGAGGCAATGATTACTTCGACACAGTGCCACATGGCTTTGAAACC1030.3405859400833278No Hit
GAAACGGGGAGTACAGGGAAACTTGCATATCGTCAAACAAATTTAGAAGCCC1010.33397262085840884No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA970.3207459824085709No Hit
CATGCGGGCAACGACACAAGAAACACAACTTCAGACTTGCAGAAAACCAGTG970.3207459824085709No Hit
AGTCCAGGGTGTTGGAAATAAAATATAAGACATTGATCTAGATATGAGGTTT970.3207459824085709No Hit
CTCTGCCGGGGGCGACGGCGGCGGCCGTCCTCATCCCGGCGCTTGAGAGGAC960.31743932279611137No Hit
CTTCATGGGGACGCTCTTCCGATTTGATACGGGGGCTCTTCCACAGGAGGCC950.31413266318365185No Hit
ATCCAAGGGGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGC950.31413266318365185No Hit
TTATGGGGCACCCGCCATCCACCAGTCCTAACACCTCCCGACCAGGAAGTCA940.3108260035711924No Hit
ACACAAGGGATGTTAAGTTTAAGATGGAGGTGCCAGGTAATTGGTTACAAGA940.3108260035711924No Hit
TAGATAGGGGCTGGGATTACAGGCTTGAGCCACTCCGCCCTGCCTGTTAATT940.3108260035711924No Hit
TTACCGGGAACTCTGGAAATCACGTGTGTGGGGAGATGGGGACGCTTCCCAT920.3042126843462734No Hit
ACGAAGGGGCTGGCTGCCAATCCCATATACACACATTATAAGCAAAGATGCC920.3042126843462734No Hit
CTTACGCGGGGGAGGAAGGGGAGCTGAGGTCCGGGGCCCAGTTTTCCGGAGT920.3042126843462734No Hit
TCAGTTGGGGGTTGGCTGAGGCGGGAGGATTACCTGTGTTTAGGAGTTTGAG910.3009060247338139No Hit
CAATCTGGGGGGCTCGTGGGGCGCCTGGAGTGAGGGTTCTGGTTCCCGCCGG910.3009060247338139No Hit
ATCCTAGGGGGGAAGCGTTGGAGGACATTCCCTGTTGACTGCGTCGCGATGT900.29759936512135443No Hit
AATCATGGGCGATCTTAGCTTGGGATGAGGTCTTTTTTTAAAATACCACTTT890.2942927055088949No Hit
GGCACTGGGAGAACCGGTGGAGCAGCGACCCCTGAGCAGTGTTCTCTGTGCT890.2942927055088949No Hit
GAACGAGGGATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGC880.2909860458964354No Hit
GGCTGTTAAAAGGGGAAAGTTATTCTGAGACACAATCCAAAAGAGAATGGTA870.28767938628397594No Hit
AATAGGGGGAACTTTTCTAAGGAATGATACATAGAAAAGATTTTATTTTAAA860.2843727266715164No Hit
GCCAATGGGATGCAATTTCCACTATCAATAATTTAACTTCTTTGCTGCAGAA860.2843727266715164No Hit
AAGTGGGTGGTGGCTCCTCTGACAATATTAGGGGGTCCTCTTCTCATCAGAG860.2843727266715164No Hit
CCGTTAGGGATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTC860.2843727266715164No Hit
TGCAACGGGAGTTCCGCCATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCA850.28106606705905696No Hit
CGTTAGGGGAAGATAGAACCTTTAGATACTCCAACTCACCAAAATGTAAAAA840.27775940744659744No Hit
ACTGCAGGGATGGCCGCTACTTCAGGAGCTGCCCCATCTCAGGCAGGGCCGT840.27775940744659744No Hit
TCAATCGGGTGAATCTAACTTTGTTTTTGTTTTGTTTTTTTCCTTATGATTA820.27114608822167846No Hit
TCTGCGGGGCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT820.27114608822167846No Hit
CGTAGCGGGCAGAGAAACCAGCTTCCTTCAGAGAGCAGTGCTTTTGGCGGGG820.27114608822167846No Hit
GACGATGGGGTTAAAAAGAAACATAGTGTTGCCCTTTTTCTTAAAGCATCAG810.26783942860921894No Hit
TCTTGAGGGGGAGGAGGAGTTGAGAGAACGGAGCGGACGCCATGGCGACCAA810.26783942860921894No Hit
GGTCACGGGATATAGGAATGTCCTCATGTTTCTCACTGAACTTTTGAAGTAG800.2645327689967595No Hit
ATCAAGGGGATGAGGTCTTTTTTTTAAAATACCACTTTTAGTGTCAGTGAAC800.2645327689967595No Hit
AGAATGGGGTGTCATTTTTTATTAATTTACTGTGAAAACTATTACTGTTCAA790.26122610938429996No Hit
ATGTGCGGGCTTGAGAGGGGGTTCTAAGCAATGCAAATGTCTAATTGTCCCC790.26122610938429996No Hit
AGTTTGGGGTGACACTGGCTATGAACACTATGATGCTTACAACCTCTCTGCC780.2579194497718405No Hit
ATTCATGGGAATTTTACAAATGGCTTTTTACAATTGGTTTCTCATAATCATG770.254612790159381No Hit
CAAACCGGGACCCACACCCAGACCTCTTGAGTAAGGACAGAAACGTTATCAT760.2513061305469215No Hit
GGTATAGGTCTCAGGAATTTGTCAGAAAAAAGAATAAAATCACCTGAGACTC750.247999470934462No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT750.247999470934462No Hit
GCACATGGGGTGACGGGGGCTGTTGGGGGCCAGCAGGAGGATGAGGACAGCA740.24469281132200252No Hit
GCCCATGGGTACAATGGCTCCAAAGAGATGCAATAAGCAACATTTAAGACTT740.24469281132200252No Hit
AGGCGTGGGGGGTGAAATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCA720.2380794920970835No Hit
TCGCATGGGACCAGGGGTCTAATCAGGCCTGTGTCTGCCTCCTTCTTGAATA720.2380794920970835No Hit
TCAATGGGGTCACTCCCTGAAGTTCATTGCCACCCTTCAGTACATCGTGGGC710.23477283248462405No Hit
TGTGCAGTGAACAAGACAAAGTCCCTGCTGTCATGGAACTTTCCATCTAGTG700.23146617287216456No Hit
ATCACCGGGAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCA690.22815951325970504No Hit
TTTAGGGGGACTATGCACCCTATCCAAATGGCTAGGTCACAACAGTGATTAA680.22485285364724555No Hit
TATGCTGGGTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAAC680.22485285364724555No Hit
CTTCTAGGGCCAGTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTG680.22485285364724555No Hit
GAGGCAGGAAAATCGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGA670.22154619403478604No Hit
GAATCTGTTTCTATGTCCAAACCTGGTAAATTTTATAATGTCATATTGTTTG670.22154619403478604No Hit
CAGCCTGGGCAACAAGAGCGGGACTCCATCTCAAAAAAAATTAAAAAAAAGA670.22154619403478604No Hit
CACCCCGGCCCACTCCAGCGGCGACTTTGAGGGATTCCCTCTCTGGCGGCCT660.21823953442232655No Hit
GCTACTGGGGAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGAGCGAG660.21823953442232655No Hit
GGACTCGGGGGGAACGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA660.21823953442232655No Hit
AAACTTGGGGCCTGATGCTAAAGTTTTTAAAAATTACTTAAAGGTGTTCTTC660.21823953442232655No Hit
CTGCACTAGGGCAGTGTATCAGCGACAAACAGTTTCAGCACCTCAGTGTCTC650.21493287480986706No Hit
CGCCATGGGAACTTCTGACCTTGTGATCCACCCACCTTGGTCTCCCAAAGTG620.2050128959724886No Hit
GCCTCAAGGGGTGAAGTAATTCCAGAAAGCACCAGTGCAGTAACAAACATTC620.2050128959724886No Hit
GCGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA610.2017062363600291No Hit
GTAGCCAGGCCATATAGGTATCCACAGAAAGGAGATCAGATTTCACTCTGAG610.2017062363600291No Hit
TCAGGGGGCTGGGGATCCTTGGATGGATTTCAGAGATTCTGTTGAATCCCCT610.2017062363600291No Hit
GGCAGGGCACCATTTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTA610.2017062363600291No Hit
GAGAAAGGGATGTAGTCAATATAACAAACAAGAGATAGCCTTTGAAATGGCA600.19839957674756958No Hit
TATTCCGGGGTGGGCTTTCTGAGGCGATGCCCGTTGGGCAAACTTAGGTTTA590.19509291713511012No Hit
ATACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.1917862575226506No Hit
CAGCAGTGGTATCAACGCAGAGTACGGGTGGCCCATCGCCATTGCAACGGCT580.1917862575226506Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGATTAGGGAATGCTTATTCTGAAAATCCTAGGAACCTTAAGAATGATGCTA560.18517293829773163No Hit
CGTGCAGGGAAATGGGAAATGTTATGACACAATCGAGGAGCTGAATAGAAGC560.18517293829773163No Hit
ACTGCTGGGGGCAGACAATGATTGAAGCTCACGTTGATGTCAAGACTACCGA550.18186627868527214No Hit
TAGTATGGGATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAA540.17855961907281265No Hit
GCGCTGGGGTATGTTTCCTTCCTGGGAACAAGATGCTAGCGCGGTGCACCGG540.17855961907281265No Hit
GCCATAGGGAGTTTCTGATGTTTCAGGCAGGAGTGGGGTAAATAAGTGTGAC530.17525295946035313No Hit
TCGACGGGGTAAATAAATCCTGCACCAAATCCCCAGGGGGAGAAATTAACAA530.17525295946035313No Hit
GTAGGGGGGAACCGGATCGGTGACTGTGGAGGGCGAGCTGAGCCCTGGCCGC520.17194629984789367No Hit
TTATATGGGTGAATCCAAATGAGATAATTATACCACCCACTGAGGCACCTTA520.17194629984789367No Hit
TTCATAGGGAAGTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCAC520.17194629984789367No Hit
GAATAAGGGTTGGTCACCAGTTGATATGTTGTCTTTGTAGAAAAGAATTCAA510.16863964023543415No Hit
TTATTGGGGAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCT510.16863964023543415No Hit
AAGGCTGGGACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGA510.16863964023543415No Hit
ACATACGGGGGCTGACTCTGGTTCAGTATTCTCTTAGGTGATTATATTCTCT510.16863964023543415No Hit
GATAAAGGGGAGGAGCTGGTGGCGTCGGCAGGTTCGAGGCGATTCGAGCTCC480.15871966139805568No Hit
CATTTAGGGACTTTGGTTTAAAAACAAAAACCCTTATATACATGGAATAGTT480.15871966139805568No Hit
TGGCTTGGGCGAGGAGCGCTGATGACGTGTGCTGTGGTCCTGTGGGTGCTGC470.1554130017855962No Hit
ATAGGGGGGGCCGAGCCACCTCGGATCCCACTGATTGGCCAGCCGAGCGAGA460.1521063421731367No Hit
ACATGGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCGCT450.14879968256067722No Hit
CAATAAGGGTATGCATATGATCTTTAATTATTGAAGAAAATAAAGTGAGGAC440.1454930229482177No Hit
TGAGGGTGGCATTCTGCTGACCACAGCACTCCAACCAAAAGCCAAAAACCAA440.1454930229482177No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA430.1421863633357582No Hit
GTCAAGGGGGTTTCATATTAAAGAATGTATTTGTAAAATGTAACATGATCAG430.1421863633357582No Hit
TCTTGGGGGAGGACACAACTTATACAGTATAGGAAGCATTATACTTTTTTGA430.1421863633357582No Hit
TTTGACGGGACTGGGGAAAAACCATACAAATGTGAAGAATGTGGCAAAGCCT420.13887970372329872No Hit
GCATCAGGGCATCTTGATCTGTGCACTAGTCTGTGATTATTAGGGTGTTAGT410.13557304411083923No Hit
TCTGCAGGGGTAGACTCAGTTACCCAGTATGCCTTAATTTTTTAATGTGTGA410.13557304411083923No Hit
TTGTCAGGGACAAGTATACTTATTTCTTGGTGCGTAATCTTTACATGATGTC400.13226638449837974No Hit
TCGCTAGGGAGGATGGCTAGGAAAGGGAAGCCTGACTGCTCGGTCAGGAGGG400.13226638449837974No Hit
TCAAGTGGGGAGGCCACTTCCGGCGTAGCCATGGCGGCTAACGCTACTACCA400.13226638449837974No Hit
TCTGCTGGGTCTTCCGATCTGCATGTGGGCAAAAGGTGAACTATTGGACACA400.13226638449837974No Hit
AAGGCCGAGGCAGGTGGATCACAAGGTCAGGAGTTGGAGACCAGCCTGACCA390.12895972488592025No Hit
TATAGTGGGTGAGATACCTTTTACAATGATTTAAGAAAAATCAATTCTGTTA380.12565306527346076No Hit
CTAGTGGGGGATTCAACCAGGTGTTGAAGGCACGACAGCCACTGCTCTACGA380.12565306527346076No Hit
TAACAGGGGCTTGTTTTACCTTGGCTCATTAGTGTTTAAAAATGTACTGATG380.12565306527346076No Hit
TTTTTTGGGATCTGTGACTATTATATAGGGATCACAAAAGTGTCACATATTA380.12565306527346076No Hit
GTAGGTGGGAGGGCTTGGGGAGAGGGAGGACACATCTGGAGGAAATGGCCTT370.12234640566100126No Hit
TACACTGGGGTGTCCGGGATGGATGGACAGCTTCTTTGCCCAAAATGCAGTG370.12234640566100126No Hit
TCAGAAGGGAAGTATTTTTTCATCACTGAAAGGTTTTTTTTTTTTATCACTA370.12234640566100126No Hit
AGTTATGGGCACCAATGACATGTTCAGGCTCGCCCTGCACAACTTTGGCTGA370.12234640566100126No Hit
TTTCAGGGGTCCTAAAAACAGACCATTTTCCTTAACTTGCATCAGTTTTGGT370.12234640566100126No Hit
GATCTTGAGGGGGGCGTCTCCAATATGACTATTAAAACTGTCACCTCTGGAA360.11903974604854176No Hit
GCTAAATACGGGTTATGGAAATCTTTAGCTTTTAATCATTTTTACCAATTTG360.11903974604854176No Hit
ACCCTGGTGTTAGAAACCGTTTCATCTCTTATTGTAAATTTGAGTGCTTGTT350.11573308643608228No Hit
CGGTTGGGGATTCTTGTATGTGTAAAATGTTAACCTAGCCATAAACATTAGC350.11573308643608228No Hit
TAAATTGGGACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA340.11242642682362278No Hit
GTTGTAGGGGCATGATCAAGCTAGGTCTAGGTATTGATGAAGATGAAGTGGC340.11242642682362278No Hit
TTTGTAGGGGGGTCCCGGTGCGTGGGGCCCGGAACCAGCTGCGCATGTACCT340.11242642682362278No Hit
CCCCAGGGTACCTTTTCAGTAACATTTTATACATCTACTTGTCAATGTATTT330.10911976721116327No Hit
TCACAGGGGGCACTGGGAGCCGTGCCATGTTTCAGGCCCTGTCCTGGCTCTG320.1058131075987038No Hit
ATAAGTGGGGGTGGGGGAGAAGGGGGTGGGTGCTGCTTGTGGTTTAGTCTTT320.1058131075987038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)