FastQCFastQC Report
Sat 31 Dec 2022
SRR5844066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30317
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC4411.454629415839298No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT4301.4183461424283406No Hit
TCGCCAGGGAGCCTTTAATTAGAATCCTAGTTTTCTCTCTAATCCAGTGGCA1590.5244582247583864No Hit
TCTAAAGGGCAGCTACTATGGGTGATCTCAACATGATTTTTATTTGAAAGTG1540.5079658277534056No Hit
AGTTTTGGGAAGCCAACAACAGGGTCTTGAGATAACCTTTGAAGCTTATTGT1450.47827951314444045No Hit
ACACAAGGGATGTTAAGTTTAAGATGGAGGTGCCAGGTAATTGGTTACAAGA1380.4551901573374675No Hit
TGGTGGGACCTAGAAATCCCTCTGGACACTTGTGGGTTCTTTAGGGTTTGAG1330.4386977603324867No Hit
AACACAGGGGCCCCATAGTTCAGCTTGTGAAACTTTCACGTCGTTGTGTTGG1330.4386977603324867No Hit
CGATATGGGCACCTCAGGCCGCTGCTCAGATGCCTCTGCTGGAGCTGGCACT1320.4353992809314906No Hit
CGGCGGCGCGTCTGGAGGAGGCTGCAGCAGCGGAAGACCCCAGTCCAGATCC1310.4321008015304944No Hit
TTATGGGGCACCCGCCATCCACCAGTCCTAACACCTCCCGACCAGGAAGTCA1300.4288023221294983No Hit
AGTCCAGGGTGTTGGAAATAAAATATAAGACATTGATCTAGATATGAGGTTT1290.42550384272850217No Hit
TTGAGCCGGGGCAGAGCTCGAGGAAGAACAGTCCCAGGGCTCCACCACAAAT1280.42220536332750597No Hit
CGCTATGGGGGGCGCACGCAGCCGCACTGGGGGTGCAAGACTACCTCCTGTT1260.41560840452551373No Hit
AGCTGGGACTCAGAGGGTGTCTCTGCAGTCTGGTGGATCTTGTCCATTGGGC1240.4090114457235215No Hit
GAACGAGGGATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGC1230.4057129663225253No Hit
ATCCAAGGGGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGC1230.4057129663225253No Hit
AGACACGGGGATTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAG1200.39581752811953685No Hit
TTAGTGGGGGGGTGCGGGTGCCCGGGTGAGGGGCGGAGCTGGGGGCATGGCG1180.3892205693175446No Hit
CATGCGGGCAACGACACAAGAAACACAACTTCAGACTTGCAGAAAACCAGTG1170.38592208991654847No Hit
CGGGATGGGCAGATGCTTTTTCGTGTACATGATACTAGTAGACACTTTTCTC1160.3826236105155523No Hit
AATTGGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGT1140.37602665171356003No Hit
GTTACAGGGTCCTATAATGGGAGCATCACCACTTATTAAAACACACATAGAA1130.3727281723125639No Hit
TTGATGGGGCAGCTGATGGACACTTGCGTTGCTTCCACCTTTGGGTTCTTGT1120.3694296929115678No Hit
ATGTGCGGGCTTGAGAGGGGGTTCTAAGCAATGCAAATGTCTAATTGTCCCC1100.36283273410957545No Hit
TTGCTTGGGAAGAAACTGAAAGCTGTATGTCAACTTTATAAACATAATTCAA1090.35953425470857936No Hit
GACGCGGAGCCTTGAAGACTGTGCTGGTGAGAAGAAGTCTGGGTGGGAGATG1070.35293729590658707No Hit
CAATCTGGGGGGCTCGTGGGGCGCCTGGAGTGAGGGTTCTGGTTCCCGCCGG1070.35293729590658707No Hit
CCGTTAGGGATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTC1070.35293729590658707No Hit
ATTTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1060.3496388165055909No Hit
CAAACCGGGACCCACACCCAGACCTCTTGAGTAAGGACAGAAACGTTATCAT1060.3496388165055909No Hit
TGAACCCGGGAGGCAGAGGTTGCAGTGGGCTGAGATCGCACCACTGCACTCC1050.3463403371045948No Hit
AGTTTGGGAGCACCTAGTGCGAGACAGTGTGTTCCAGCGCTGCGGCCTCCGC1050.3463403371045948No Hit
ACGAAGGGGCTGGCTGCCAATCCCATATACACACATTATAAGCAAAGATGCC1040.34304185770359863No Hit
GATCACGGGTGTGGAAAAAAACAAGCTCACTTTGGGATTACCCACCCCGAAA1030.33974337830260254No Hit
CTCCAGGGGAGGCAATGATTACTTCGACACAGTGCCACATGGCTTTGAAACC1020.33644489890160634No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA1010.3331464195006102No Hit
CTTCATGGGGACGCTCTTCCGATTTGATACGGGGGCTCTTCCACAGGAGGCC980.32325098129762175No Hit
ATCAAGGGGATGAGGTCTTTTTTTTAAAATACCACTTTTAGTGTCAGTGAAC980.32325098129762175No Hit
AATCATGGGCGATCTTAGCTTGGGATGAGGTCTTTTTTTAAAATACCACTTT980.32325098129762175No Hit
AAGTGGGTGGTGGCTCCTCTGACAATATTAGGGGGTCCTCTTCTCATCAGAG980.32325098129762175No Hit
CGTTAGGGGAAGATAGAACCTTTAGATACTCCAACTCACCAAAATGTAAAAA970.31995250189662566No Hit
CTTAAAGGGAACTCCCTGAATGACGACATCAGCCTCTACAAGAGCTTCCTCC970.31995250189662566No Hit
GAAACGGGGAGTACAGGGAAACTTGCATATCGTCAAACAAATTTAGAAGCCC970.31995250189662566No Hit
CTCTGCCGGGGGCGACGGCGGCGGCCGTCCTCATCCCGGCGCTTGAGAGGAC960.3166540224956295No Hit
AGAATGGGGTGTCATTTTTTATTAATTTACTGTGAAAACTATTACTGTTCAA930.3067585842926411No Hit
TCTGCGGGGCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT930.3067585842926411No Hit
CTTCTAGGGCCAGTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTG920.30346010489164493No Hit
TTACCGGGAACTCTGGAAATCACGTGTGTGGGGAGATGGGGACGCTTCCCAT910.30016162549064884No Hit
GGCTGTTAAAAGGGGAAAGTTATTCTGAGACACAATCCAAAAGAGAATGGTA910.30016162549064884No Hit
GCTACTGGGGAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGAGCGAG890.2935646666886565No Hit
ATCCTAGGGGGGAAGCGTTGGAGGACATTCCCTGTTGACTGCGTCGCGATGT880.2902661872876604No Hit
CGTAGCGGGCAGAGAAACCAGCTTCCTTCAGAGAGCAGTGCTTTTGGCGGGG870.28696770788666426No Hit
GGCACTGGGAGAACCGGTGGAGCAGCGACCCCTGAGCAGTGTTCTCTGTGCT840.2770722696836758No Hit
TGTCATGGGAAGTCAACCTAGAACTATACACAAATACAAACATGCCTAAAAT830.2737737902826797No Hit
GGACTCGGGGGGAACGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA830.2737737902826797No Hit
CTTACGCGGGGGAGGAAGGGGAGCTGAGGTCCGGGGCCCAGTTTTCCGGAGT820.2704753108816836No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT820.2704753108816836No Hit
ACATACGGGGGCTGACTCTGGTTCAGTATTCTCTTAGGTGATTATATTCTCT820.2704753108816836No Hit
TCAGTTGGGGGTTGGCTGAGGCGGGAGGATTACCTGTGTTTAGGAGTTTGAG810.2671768314806874No Hit
GGTCACGGGATATAGGAATGTCCTCATGTTTCTCACTGAACTTTTGAAGTAG800.26387835207969124No Hit
TACGAAGGGATTTATTAATATGTAAAAGAGTGATAAACAAGGGAAAATAAAG800.26387835207969124No Hit
TCAATGGGGTCACTCCCTGAAGTTCATTGCCACCCTTCAGTACATCGTGGGC800.26387835207969124No Hit
TGTGCAGTGAACAAGACAAAGTCCCTGCTGTCATGGAACTTTCCATCTAGTG790.26057987267869515No Hit
GCACATGGGGTGACGGGGGCTGTTGGGGGCCAGCAGGAGGATGAGGACAGCA780.257281393277699No Hit
GGTATAGGTCTCAGGAATTTGTCAGAAAAAAGAATAAAATCACCTGAGACTC760.2506844344757067No Hit
TCGCATGGGACCAGGGGTCTAATCAGGCCTGTGTCTGCCTCCTTCTTGAATA760.2506844344757067No Hit
AGTTTGGGGTGACACTGGCTATGAACACTATGATGCTTACAACCTCTCTGCC760.2506844344757067No Hit
TGCAACGGGAGTTCCGCCATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCA750.24738595507471056No Hit
TCAATCGGGTGAATCTAACTTTGTTTTTGTTTTGTTTTTTTCCTTATGATTA730.24078899627271827No Hit
GCCAATGGGATGCAATTTCCACTATCAATAATTTAACTTCTTTGCTGCAGAA730.24078899627271827No Hit
ACTGCAGGGATGGCCGCTACTTCAGGAGCTGCCCCATCTCAGGCAGGGCCGT730.24078899627271827No Hit
TAGATAGGGGCTGGGATTACAGGCTTGAGCCACTCCGCCCTGCCTGTTAATT720.23749051687172212No Hit
ATTCATGGGAATTTTACAAATGGCTTTTTACAATTGGTTTCTCATAATCATG700.23089355806972986No Hit
CGCCATGGGAACTTCTGACCTTGTGATCCACCCACCTTGGTCTCCCAAAGTG700.23089355806972986No Hit
CACCCCGGCCCACTCCAGCGGCGACTTTGAGGGATTCCCTCTCTGGCGGCCT690.22759507866873374No Hit
AGGCGTGGGGGGTGAAATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCA690.22759507866873374No Hit
GCCTCAAGGGGTGAAGTAATTCCAGAAAGCACCAGTGCAGTAACAAACATTC690.22759507866873374No Hit
GGCAGGGCACCATTTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTA690.22759507866873374No Hit
GCGCTGGGGTATGTTTCCTTCCTGGGAACAAGATGCTAGCGCGGTGCACCGG680.22429659926773757No Hit
CTGCACTAGGGCAGTGTATCAGCGACAAACAGTTTCAGCACCTCAGTGTCTC680.22429659926773757No Hit
ATACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA670.22099811986674142No Hit
TATGCTGGGTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAAC670.22099811986674142No Hit
CAGCCTGGGCAACAAGAGCGGGACTCCATCTCAAAAAAAATTAAAAAAAAGA670.22099811986674142No Hit
TTTAGGGGGACTATGCACCCTATCCAAATGGCTAGGTCACAACAGTGATTAA650.21440116106474916No Hit
GAATCTGTTTCTATGTCCAAACCTGGTAAATTTTATAATGTCATATTGTTTG650.21440116106474916No Hit
TAGTATGGGATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAA640.21110268166375298No Hit
GTAGGGGGGAACCGGATCGGTGACTGTGGAGGGCGAGCTGAGCCCTGGCCGC630.20780420226275687No Hit
ACTGCTGGGGGCAGACAATGATTGAAGCTCACGTTGATGTCAAGACTACCGA630.20780420226275687No Hit
GACGATGGGGTTAAAAAGAAACATAGTGTTGCCCTTTTTCTTAAAGCATCAG620.20450572286176075No Hit
TATTCCGGGGTGGGCTTTCTGAGGCGATGCCCGTTGGGCAAACTTAGGTTTA620.20450572286176075No Hit
TCAGGGGGCTGGGGATCCTTGGATGGATTTCAGAGATTCTGTTGAATCCCCT600.19790876405976843No Hit
GAGGCAGGAAAATCGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGA590.1946102846587723No Hit
GTAGCCAGGCCATATAGGTATCCACAGAAAGGAGATCAGATTTCACTCTGAG590.1946102846587723No Hit
AAACTTGGGGCCTGATGCTAAAGTTTTTAAAAATTACTTAAAGGTGTTCTTC590.1946102846587723No Hit
TTCATAGGGAAGTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCAC590.1946102846587723No Hit
TTATTGGGGAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCT590.1946102846587723No Hit
TGGCTTGGGCGAGGAGCGCTGATGACGTGTGCTGTGGTCCTGTGGGTGCTGC580.19131180525777616No Hit
CGTGCAGGGAAATGGGAAATGTTATGACACAATCGAGGAGCTGAATAGAAGC570.18801332585678002No Hit
CAGCAGTGGTATCAACGCAGAGTACGGGTGGCCCATCGCCATTGCAACGGCT560.1847148464557839Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGATTAGGGAATGCTTATTCTGAAAATCCTAGGAACCTTAAGAATGATGCTA550.18141636705478772No Hit
GCCCATGGGTACAATGGCTCCAAAGAGATGCAATAAGCAACATTTAAGACTT550.18141636705478772No Hit
GAGAAAGGGATGTAGTCAATATAACAAACAAGAGATAGCCTTTGAAATGGCA540.1781178876537916No Hit
AATAGGGGGAACTTTTCTAAGGAATGATACATAGAAAAGATTTTATTTTAAA530.17481940825279546No Hit
CATTTAGGGACTTTGGTTTAAAAACAAAAACCCTTATATACATGGAATAGTT510.16822244945080317No Hit
GTCAAGGGGGTTTCATATTAAAGAATGTATTTGTAAAATGTAACATGATCAG500.16492397004980705No Hit
AAGGCCGAGGCAGGTGGATCACAAGGTCAGGAGTTGGAGACCAGCCTGACCA500.16492397004980705No Hit
TCTTGAGGGGGAGGAGGAGTTGAGAGAACGGAGCGGACGCCATGGCGACCAA500.16492397004980705No Hit
GCCATAGGGAGTTTCTGATGTTTCAGGCAGGAGTGGGGTAAATAAGTGTGAC490.16162549064881088No Hit
TGAGGGTGGCATTCTGCTGACCACAGCACTCCAACCAAAAGCCAAAAACCAA490.16162549064881088No Hit
TTTCAGGGGTCCTAAAAACAGACCATTTTCCTTAACTTGCATCAGTTTTGGT490.16162549064881088No Hit
TTTGACGGGACTGGGGAAAAACCATACAAATGTGAAGAATGTGGCAAAGCCT490.16162549064881088No Hit
GATCTTGAGGGGGGCGTCTCCAATATGACTATTAAAACTGTCACCTCTGGAA480.15832701124781476No Hit
ATCACCGGGAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCA480.15832701124781476No Hit
TTGTCAGGGACAAGTATACTTATTTCTTGGTGCGTAATCTTTACATGATGTC470.15502853184681864No Hit
GAATAAGGGTTGGTCACCAGTTGATATGTTGTCTTTGTAGAAAAGAATTCAA470.15502853184681864No Hit
GTTGTAGGGGCATGATCAAGCTAGGTCTAGGTATTGATGAAGATGAAGTGGC450.14843157304482635No Hit
ACATGGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCGCT440.1451330936438302No Hit
GCATCAGGGCATCTTGATCTGTGCACTAGTCTGTGATTATTAGGGTGTTAGT430.14183461424283406No Hit
GATAAAGGGGAGGAGCTGGTGGCGTCGGCAGGTTCGAGGCGATTCGAGCTCC430.14183461424283406No Hit
AGTTATGGGCACCAATGACATGTTCAGGCTCGCCCTGCACAACTTTGGCTGA430.14183461424283406No Hit
TTGCATGGGAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTC420.1385361348418379No Hit
CAGACAGGGATGTCCTTGACTACAACAGTAAAAAAATTGGAAACAACCTAAG420.1385361348418379No Hit
ATAGGGGGGGCCGAGCCACCTCGGATCCCACTGATTGGCCAGCCGAGCGAGA420.1385361348418379No Hit
GCGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA400.13193917603984562No Hit
AACAAGGGCCGATCTGGCCACGGGCACTAAATTTATAACTTTTTGAAACAAA400.13193917603984562No Hit
TAACAGGGGCTTGTTTTACCTTGGCTCATTAGTGTTTAAAAATGTACTGATG390.1286406966388495No Hit
TCTGCAGGGGTAGACTCAGTTACCCAGTATGCCTTAATTTTTTAATGTGTGA380.12534221723785335No Hit
CGGTTGGGGATTCTTGTATGTGTAAAATGTTAACCTAGCCATAAACATTAGC370.12204373783685721No Hit
TCGACGGGGTAAATAAATCCTGCACCAAATCCCCAGGGGGAGAAATTAACAA370.12204373783685721No Hit
TCTTGGGGGAGGACACAACTTATACAGTATAGGAAGCATTATACTTTTTTGA370.12204373783685721No Hit
GTAGGTGGGAGGGCTTGGGGAGAGGGAGGACACATCTGGAGGAAATGGCCTT360.11874525843586106No Hit
TAAATTGGGACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA360.11874525843586106No Hit
TTTGTAGGGGGGTCCCGGTGCGTGGGGCCCGGAACCAGCTGCGCATGTACCT360.11874525843586106No Hit
TACACTGGGGTGTCCGGGATGGATGGACAGCTTCTTTGCCCAAAATGCAGTG350.11544677903486493No Hit
AAGGCTGGGACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGA350.11544677903486493No Hit
ATTAGCGGGTAACACCAAAAGTGAACCCAAATGTAAACTTAGGACTTTGAAG350.11544677903486493No Hit
TTTATGGGGAACAATGTATGTTTGGAGTTCCAAATCTGCAAGTGGCCAACCT340.11214829963386878No Hit
TTTCTCGGGCTAGCAGCTGCTGAGAAATTCAAAGTTCAAGGTGAAGCTGTCT340.11214829963386878No Hit
GAAATGGGGCGATCTTGCTTGCCTTCATCATCACTTGGACCCCATACAACAT340.11214829963386878No Hit
TGTATAGGGCGCTCTTCCGATCTTTGCAGGGGTGCAAAAATCTATGAAGTTA340.11214829963386878No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA330.10884982023287265No Hit
TGCTCTGTTGCCCGGGCTGGAGTGCAGTTGTGTGATCTTGGCTCACTGCAAC330.10884982023287265No Hit
CAATAAGGGTATGCATATGATCTTTAATTATTGAAGAAAATAAAGTGAGGAC330.10884982023287265No Hit
TCAAGTGGGGAGGCCACTTCCGGCGTAGCCATGGCGGCTAACGCTACTACCA330.10884982023287265No Hit
TTTTTTGGGATCTGTGACTATTATATAGGGATCACAAAAGTGTCACATATTA330.10884982023287265No Hit
TTATATGGGTGAATCCAAATGAGATAATTATACCACCCACTGAGGCACCTTA320.10555134083187649No Hit
ATTAATGGGAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACT320.10555134083187649No Hit
CTAGTGGGGGATTCAACCAGGTGTTGAAGGCACGACAGCCACTGCTCTACGA310.10225286143088037No Hit
TCTGCTGGGTCTTCCGATCTGCATGTGGGCAAAAGGTGAACTATTGGACACA310.10225286143088037No Hit
GCTAAATACGGGTTATGGAAATCTTTAGCTTTTAATCATTTTTACCAATTTG310.10225286143088037No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)